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The map label for this gene is yhfT [H]

Identifier: 86360747

GI number: 86360747

Start: 12135

End: 13652

Strand: Direct

Name: yhfT [H]

Synonym: RHE_PF00013

Alternate gene names: 86360747

Gene position: 12135-13652 (Clockwise)

Preceding gene: 86360746

Following gene: 86360748

Centisome position: 1.89

GC content: 63.97

Gene sequence:

>1518_bases
ATGCCGATCCATCGAGAGCTTCTTCGACACGTCACGGAAAGACCGGAAAAGCCGGCGGTTGTGATCGACGGCGGCTCCCT
GAGCTATGGCGAACTCTATAGCCGCGCCTGCGCGATCCACCGGTTCATCCAAGCACTTCCGCGCTCGAAACGCCGTTCAT
TCGATCTGCCCGGCGTCGAGAAGCTTCTGGCGGTCAGCCTCGGCAATCACATCGGCTTTGCCGGGTATTTCGTTGCCGCC
ACGGCGCTGCCGAACGCCTGCGCGGTCATCGATCCAATGATGCCGGAAGCGAGAATCGAGCGCATCGTTGAGCGCCTGGC
GCCTGACGTGCTTGTCGTCAACGATGCTGCGTGCCCGAGCGCCGAGATCGGCCGCGGGCTCGGCATTCCTGTGATCGTCG
CCGGAACCGAGCCCTTCTCCCTTACAGCAAAAACAGACGCCTTTCCGGTCGATGCCGAGGGAATTTTCCTGATCGGCTTC
ACCTCGGGCACGACAGCCGAGCCGAAGGCCTATTATCGCTCGCGCCAGCAGTGGCGCCGGAGCCTTGAGCGGGGGCGTCT
CGTTTTCGAACTCCAGGACGCACCCTCGACGCTGTGCCCGGGAGCGCTCGCGCATGGGCTCGCGCTTTATGCGCTGGTCG
AGGCGCTCGATGCCGGAGCAACCTTCCATTCGGTGGGGAAATGGGATGCCGGCACGGTCGCGCAGATTCTGTCTTCCGGG
ATCGAACGGCTGGTCGCCGTGCCCACCCATGTAGCAGGCATCGCCCGGGCATGGGCGGGCGAGCCGATGACGTCCTTGCG
CGACGTGCTGACCGCCGGCTCCAAGCTGAACCTCGGCGAGATCGAATCCATGCGCCGCCTTTTTCCGAAGGCGCGCATCC
GGGAATATTACGGCGCCTCCGAGATCGGCTTCATGACGGTCTCGACGCTTGCCGGCGGCGCGAGCGATTTTCCGATCGAT
CGGGTCGGGCAAGCCTATCCCGGTATCAAGATCTCCATTCGCGACCCTGAGGAAAACGACGTCGGCACCGATGTGCCCGG
CACCATCTTCGTCGACAGCGACCTGATCGCCGACGGCTATCTCTGGGGCGACGACGGCCAGGCGTTCCGGGTCACCCCGG
CGGGCGCGACCGTCGGCGATCTCGGCGAGATCGATGCGAACGGCATGCTGCGGGTGATCGGTCGGGCAGGGGGCATGATG
ATCACGGGCGGCAACAACGTCTATCCCGCCGAGGTCGAGAGCGCACTCAAAAGCTGTCCCGACGTCGAGGATGCCGTCGT
CTTCGGGCTGCCGGATGCCTATTACGGCCAACGGATCGTCGCCGTCGTCTCGGGCGAGGTGACGGACGCGAAAATCCTCG
CCGACCATTGCGCGGGCAAGCTTGCAAGGTTCAAGATTCCCAGGCAGTTCTACCATATCGGCTCCTGGCCGATGACGAGC
AGCGGCAAGATCGCGCGCGGCCAGGTGGAAGCATCGGTCATTTCGGGAGATGATCTTGTCCTACGCCTATCAGCCTGA

Upstream 100 bases:

>100_bases
GCGCATCGGGCGGCCTCGTCTGGGTCTGCGTCGGCCTCTCCGACAGCCGCCCGACGCCGATCCCCGAGGCGACCAGGGCC
GCCTTGTCACAGGGCTGACC

Downstream 100 bases:

>100_bases
CGAGGATTGGCAGCCGGTCGTGGTCGCCGCCTACCGCACACCGATCGGCCGGGCCTTCGGCTCGCTGGCTACGGTTGCGG
CCGAAGACCTGCTTGCGCCC

Product: putative long-chain-fatty-acid--CoA ligase protein

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MPIHRELLRHVTERPEKPAVVIDGGSLSYGELYSRACAIHRFIQALPRSKRRSFDLPGVEKLLAVSLGNHIGFAGYFVAA
TALPNACAVIDPMMPEARIERIVERLAPDVLVVNDAACPSAEIGRGLGIPVIVAGTEPFSLTAKTDAFPVDAEGIFLIGF
TSGTTAEPKAYYRSRQQWRRSLERGRLVFELQDAPSTLCPGALAHGLALYALVEALDAGATFHSVGKWDAGTVAQILSSG
IERLVAVPTHVAGIARAWAGEPMTSLRDVLTAGSKLNLGEIESMRRLFPKARIREYYGASEIGFMTVSTLAGGASDFPID
RVGQAYPGIKISIRDPEENDVGTDVPGTIFVDSDLIADGYLWGDDGQAFRVTPAGATVGDLGEIDANGMLRVIGRAGGMM
ITGGNNVYPAEVESALKSCPDVEDAVVFGLPDAYYGQRIVAVVSGEVTDAKILADHCAGKLARFKIPRQFYHIGSWPMTS
SGKIARGQVEASVISGDDLVLRLSA

Sequences:

>Translated_505_residues
MPIHRELLRHVTERPEKPAVVIDGGSLSYGELYSRACAIHRFIQALPRSKRRSFDLPGVEKLLAVSLGNHIGFAGYFVAA
TALPNACAVIDPMMPEARIERIVERLAPDVLVVNDAACPSAEIGRGLGIPVIVAGTEPFSLTAKTDAFPVDAEGIFLIGF
TSGTTAEPKAYYRSRQQWRRSLERGRLVFELQDAPSTLCPGALAHGLALYALVEALDAGATFHSVGKWDAGTVAQILSSG
IERLVAVPTHVAGIARAWAGEPMTSLRDVLTAGSKLNLGEIESMRRLFPKARIREYYGASEIGFMTVSTLAGGASDFPID
RVGQAYPGIKISIRDPEENDVGTDVPGTIFVDSDLIADGYLWGDDGQAFRVTPAGATVGDLGEIDANGMLRVIGRAGGMM
ITGGNNVYPAEVESALKSCPDVEDAVVFGLPDAYYGQRIVAVVSGEVTDAKILADHCAGKLARFKIPRQFYHIGSWPMTS
SGKIARGQVEASVISGDDLVLRLSA
>Mature_504_residues
PIHRELLRHVTERPEKPAVVIDGGSLSYGELYSRACAIHRFIQALPRSKRRSFDLPGVEKLLAVSLGNHIGFAGYFVAAT
ALPNACAVIDPMMPEARIERIVERLAPDVLVVNDAACPSAEIGRGLGIPVIVAGTEPFSLTAKTDAFPVDAEGIFLIGFT
SGTTAEPKAYYRSRQQWRRSLERGRLVFELQDAPSTLCPGALAHGLALYALVEALDAGATFHSVGKWDAGTVAQILSSGI
ERLVAVPTHVAGIARAWAGEPMTSLRDVLTAGSKLNLGEIESMRRLFPKARIREYYGASEIGFMTVSTLAGGASDFPIDR
VGQAYPGIKISIRDPEENDVGTDVPGTIFVDSDLIADGYLWGDDGQAFRVTPAGATVGDLGEIDANGMLRVIGRAGGMMI
TGGNNVYPAEVESALKSCPDVEDAVVFGLPDAYYGQRIVAVVSGEVTDAKILADHCAGKLARFKIPRQFYHIGSWPMTSS
GKIARGQVEASVISGDDLVLRLSA

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=349, Percent_Identity=26.3610315186246, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI115511026, Length=357, Percent_Identity=25.4901960784314, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI13375727, Length=386, Percent_Identity=24.3523316062176, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI157311624, Length=361, Percent_Identity=25.7617728531856, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI157311622, Length=361, Percent_Identity=25.7617728531856, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI58082049, Length=351, Percent_Identity=25.0712250712251, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788107, Length=176, Percent_Identity=25, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI221142682, Length=366, Percent_Identity=25.6830601092896, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=358, Percent_Identity=26.2569832402235, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=410, Percent_Identity=25.3658536585366, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=348, Percent_Identity=24.4252873563218, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=348, Percent_Identity=24.4252873563218, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=348, Percent_Identity=24.4252873563218, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=355, Percent_Identity=24.5070422535211, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=369, Percent_Identity=25.2032520325203, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=372, Percent_Identity=24.7311827956989, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=362, Percent_Identity=26.2430939226519, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=353, Percent_Identity=26.0623229461756, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=343, Percent_Identity=26.530612244898, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24581924, Length=351, Percent_Identity=27.6353276353276, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI20130357, Length=236, Percent_Identity=24.5762711864407, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356947, Length=234, Percent_Identity=24.7863247863248, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24656500, Length=352, Percent_Identity=23.8636363636364, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24653035, Length=231, Percent_Identity=26.4069264069264, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI18859661, Length=355, Percent_Identity=21.1267605633803, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 53865; Mature: 53734

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIHRELLRHVTERPEKPAVVIDGGSLSYGELYSRACAIHRFIQALPRSKRRSFDLPGVE
CCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHH
KLLAVSLGNHIGFAGYFVAATALPNACAVIDPMMPEARIERIVERLAPDVLVVNDAACPS
HHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCC
AEIGRGLGIPVIVAGTEPFSLTAKTDAFPVDAEGIFLIGFTSGTTAEPKAYYRSRQQWRR
HHHCCCCCCCEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
SLERGRLVFELQDAPSTLCPGALAHGLALYALVEALDAGATFHSVGKWDAGTVAQILSSG
HHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IERLVAVPTHVAGIARAWAGEPMTSLRDVLTAGSKLNLGEIESMRRLFPKARIREYYGAS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
EIGFMTVSTLAGGASDFPIDRVGQAYPGIKISIRDPEENDVGTDVPGTIFVDSDLIADGY
CCCCEEHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEECCE
LWGDDGQAFRVTPAGATVGDLGEIDANGMLRVIGRAGGMMITGGNNVYPAEVESALKSCP
EECCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHCC
DVEDAVVFGLPDAYYGQRIVAVVSGEVTDAKILADHCAGKLARFKIPRQFYHIGSWPMTS
CCCCEEEEECCCHHHCCEEEEEEECCCCHHHHHHHHHHCHHHHHCCCHHHHCCCCCCCCC
SGKIARGQVEASVISGDDLVLRLSA
CCCEECCEEEEEEECCCCEEEEECC
>Mature Secondary Structure 
PIHRELLRHVTERPEKPAVVIDGGSLSYGELYSRACAIHRFIQALPRSKRRSFDLPGVE
CCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHH
KLLAVSLGNHIGFAGYFVAATALPNACAVIDPMMPEARIERIVERLAPDVLVVNDAACPS
HHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCC
AEIGRGLGIPVIVAGTEPFSLTAKTDAFPVDAEGIFLIGFTSGTTAEPKAYYRSRQQWRR
HHHCCCCCCCEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
SLERGRLVFELQDAPSTLCPGALAHGLALYALVEALDAGATFHSVGKWDAGTVAQILSSG
HHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IERLVAVPTHVAGIARAWAGEPMTSLRDVLTAGSKLNLGEIESMRRLFPKARIREYYGAS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
EIGFMTVSTLAGGASDFPIDRVGQAYPGIKISIRDPEENDVGTDVPGTIFVDSDLIADGY
CCCCEEHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEECCE
LWGDDGQAFRVTPAGATVGDLGEIDANGMLRVIGRAGGMMITGGNNVYPAEVESALKSCP
EECCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHCC
DVEDAVVFGLPDAYYGQRIVAVVSGEVTDAKILADHCAGKLARFKIPRQFYHIGSWPMTS
CCCCEEEEECCCHHHCCEEEEEEECCCCHHHHHHHHHHCHHHHHCCCHHHHCCCCCCCCC
SGKIARGQVEASVISGDDLVLRLSA
CCCEECCEEEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]