Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 86360743

GI number: 86360743

Start: 9305

End: 10216

Strand: Direct

Name: gcvA [H]

Synonym: RHE_PF00009

Alternate gene names: 86360743

Gene position: 9305-10216 (Clockwise)

Preceding gene: 86360740

Following gene: 86360744

Centisome position: 1.45

GC content: 66.67

Gene sequence:

>912_bases
ATGACCCAATCCCGCCGCACGCTGCCGCCTCTGAATGCCCTGCGCGCCTTCGAGGCCTCGGGCCGCAAGCTGAATTTCCG
CGGCGCCGCCGAAGAGCTGGGCGTCACGCAAGGGGCGGTCGCGCAGCAGGTTCGCGCGCTGGAAGACCAGCTCGGCCTCA
GACTGTTTCAACGCCTTGCCCGCGGCCTTGCGCTCACTGCCTATGGCACGGCCTATCTGGCGGATGTGACCCGCGCCTTC
GACACGCTTGCCGAAGCGACCGGGCGGCTTCTCGACCGGCCTGAAATGGTGACGATCAGCGCCACCCCCACGGTCGCCAC
CAGGCTGTTGATCCCGCGGCTCGCCGAGCTCTATGCCGCTCTTCCCGACATCGATCTGAGGACGGTCGCGACCGAGGCAC
TTGCCGATTTCGACCGCGACCAGGTTGATATCGCGGTGCGCCTCACCCGCCCGCCTTTTCCGGCCAACCTGCAGGCGCAG
ATGTTGTTCCGCCAGGAGCTGGTCGCGGTCGCAAGCCCGCATCTGGTTCAAGGCCTGACCTTGCCCTTAACCATCGAAGA
ACTTCGCAAACTGCCGCTTCTGCACGACTCGCACGATCACTGGCCGGTATTCTTGCGCACGCGCGAGAAGCTGCCCGGCG
CGGCCTTCAACCATACGACACTGGCGCTCGACGCCGCGCTTGCCGGCCAGGGCGTCGTCCTCGCCTGCCGGGCCTTCGTC
ATGGCCGATCTCGAAGCAGGGCGGCTGGTGCGGGTGACGGATGCGACGGCGACCATCGGACCCGATTACTTCCTCGTACG
CAAACGCTCGTCATCGCCGCGAAAGGCCGTCGATGCCGTCTGGGACTGGTGTGCGGCGCAGTTGTCGCTGCCGCCGGCTT
CGTCGTCGGGGAGAGAGCACCATGGACGATGA

Upstream 100 bases:

>100_bases
CTCCGATAGCTGATTTTGCACAGCATTATGACAGATGCCGGCGGGACTTTCGCGCGAGAAAGAAAGCTGCCAGACTGTAG
AAAATCTACTGGATATGCCG

Downstream 100 bases:

>100_bases
TATCGAAGACTTGAAACGCATTTACGACCGCTTCAATGCACGGGATATTGATGGCGTGTTGACAGTCCTTGCCGCGGACG
TCGCTTGGGCCAACGGGATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTQSRRTLPPLNALRAFEASGRKLNFRGAAEELGVTQGAVAQQVRALEDQLGLRLFQRLARGLALTAYGTAYLADVTRAF
DTLAEATGRLLDRPEMVTISATPTVATRLLIPRLAELYAALPDIDLRTVATEALADFDRDQVDIAVRLTRPPFPANLQAQ
MLFRQELVAVASPHLVQGLTLPLTIEELRKLPLLHDSHDHWPVFLRTREKLPGAAFNHTTLALDAALAGQGVVLACRAFV
MADLEAGRLVRVTDATATIGPDYFLVRKRSSSPRKAVDAVWDWCAAQLSLPPASSSGREHHGR

Sequences:

>Translated_303_residues
MTQSRRTLPPLNALRAFEASGRKLNFRGAAEELGVTQGAVAQQVRALEDQLGLRLFQRLARGLALTAYGTAYLADVTRAF
DTLAEATGRLLDRPEMVTISATPTVATRLLIPRLAELYAALPDIDLRTVATEALADFDRDQVDIAVRLTRPPFPANLQAQ
MLFRQELVAVASPHLVQGLTLPLTIEELRKLPLLHDSHDHWPVFLRTREKLPGAAFNHTTLALDAALAGQGVVLACRAFV
MADLEAGRLVRVTDATATIGPDYFLVRKRSSSPRKAVDAVWDWCAAQLSLPPASSSGREHHGR
>Mature_302_residues
TQSRRTLPPLNALRAFEASGRKLNFRGAAEELGVTQGAVAQQVRALEDQLGLRLFQRLARGLALTAYGTAYLADVTRAFD
TLAEATGRLLDRPEMVTISATPTVATRLLIPRLAELYAALPDIDLRTVATEALADFDRDQVDIAVRLTRPPFPANLQAQM
LFRQELVAVASPHLVQGLTLPLTIEELRKLPLLHDSHDHWPVFLRTREKLPGAAFNHTTLALDAALAGQGVVLACRAFVM
ADLEAGRLVRVTDATATIGPDYFLVRKRSSSPRKAVDAVWDWCAAQLSLPPASSSGREHHGR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=38.2758620689655, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=30.8219178082192, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788706, Length=270, Percent_Identity=27.4074074074074, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=25.0871080139373, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786401, Length=238, Percent_Identity=27.7310924369748, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI145693193, Length=257, Percent_Identity=27.2373540856031, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081978, Length=274, Percent_Identity=27.7372262773723, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI157672245, Length=190, Percent_Identity=30, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33135; Mature: 33003

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSRRTLPPLNALRAFEASGRKLNFRGAAEELGVTQGAVAQQVRALEDQLGLRLFQRLA
CCCCCCCCCCHHHHHHHHCCCCEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGLALTAYGTAYLADVTRAFDTLAEATGRLLDRPEMVTISATPTVATRLLIPRLAELYAA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
LPDIDLRTVATEALADFDRDQVDIAVRLTRPPFPANLQAQMLFRQELVAVASPHLVQGLT
CCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHCCCC
LPLTIEELRKLPLLHDSHDHWPVFLRTREKLPGAAFNHTTLALDAALAGQGVVLACRAFV
CCEEHHHHHCCCCCCCCCCCCEEEEEEHHCCCCCCCCCHHHEEHHHHCCCCHHHHHHHHH
MADLEAGRLVRVTDATATIGPDYFLVRKRSSSPRKAVDAVWDWCAAQLSLPPASSSGREH
HHCCCCCCEEEEECCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HGR
CCC
>Mature Secondary Structure 
TQSRRTLPPLNALRAFEASGRKLNFRGAAEELGVTQGAVAQQVRALEDQLGLRLFQRLA
CCCCCCCCCHHHHHHHHCCCCEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGLALTAYGTAYLADVTRAFDTLAEATGRLLDRPEMVTISATPTVATRLLIPRLAELYAA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
LPDIDLRTVATEALADFDRDQVDIAVRLTRPPFPANLQAQMLFRQELVAVASPHLVQGLT
CCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHCCCC
LPLTIEELRKLPLLHDSHDHWPVFLRTREKLPGAAFNHTTLALDAALAGQGVVLACRAFV
CCEEHHHHHCCCCCCCCCCCCEEEEEEHHCCCCCCCCCHHHEEHHHHCCCCHHHHHHHHH
MADLEAGRLVRVTDATATIGPDYFLVRKRSSSPRKAVDAVWDWCAAQLSLPPASSSGREH
HHCCCCCCEEEEECCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HGR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]