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The map label for this gene is cspA [H]

Identifier: 85860950

GI number: 85860950

Start: 3116024

End: 3116422

Strand: Direct

Name: cspA [H]

Synonym: SYN_01297

Alternate gene names: 85860950

Gene position: 3116024-3116422 (Clockwise)

Preceding gene: 85860949

Following gene: 85860953

Centisome position: 98.01

GC content: 46.37

Gene sequence:

>399_bases
ATGAGAGCTCATGTTTGTGCAGTACCCGTTTGTGACATAAAAATACAGAATAGAGAACCGCACAATCCTCATAAAGAGGG
CGTGCGGTTTTTTTGTTCTGAAAAAGACGGGCTGCCGGTTCGCTTGATTCGTGGATCATCCGGACCTATTAAAGAAATGG
GTTTCCAGAAAGGAAACCTCCAAAAGGAGTCGTACAAGATGTCAGAAGGAACAGTCAAATGGTTCAACGCGTCCAAGGGA
TTTGGTTTTATCGCACAGGACAATGGAAATGATGTATTCGTTCATTTTTCCGCCATTAAGATGGAGGGGTACAAAGCCCT
CGAAGAGGGTGCCAGAGTCCGGTTTGATGTTGTCAAGGGGAACAAAGGACCCGCCGCGGACAACGTTGAACTGCTGTAA

Upstream 100 bases:

>100_bases
GTCATTCGTCCCCTCTGAAGGCTGAGGGTGACGAATAAGTTTCAGAAAAAGAATAAAAAAAAAGTAAAAAAATTGTTTGC
ATTGGAGTTTTCATCATGGT

Downstream 100 bases:

>100_bases
TACCCGATTGAATTCAAAAGGATAACAAGACGGCAAGGAGGCATACACCTTGCCGTCTTTTCTTCTGTCGCACAGAGCAG
CAGTTTTCTGCCCTGTCGTC

Product: cold shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MRAHVCAVPVCDIKIQNREPHNPHKEGVRFFCSEKDGLPVRLIRGSSGPIKEMGFQKGNLQKESYKMSEGTVKWFNASKG
FGFIAQDNGNDVFVHFSAIKMEGYKALEEGARVRFDVVKGNKGPAADNVELL

Sequences:

>Translated_132_residues
MRAHVCAVPVCDIKIQNREPHNPHKEGVRFFCSEKDGLPVRLIRGSSGPIKEMGFQKGNLQKESYKMSEGTVKWFNASKG
FGFIAQDNGNDVFVHFSAIKMEGYKALEEGARVRFDVVKGNKGPAADNVELL
>Mature_132_residues
MRAHVCAVPVCDIKIQNREPHNPHKEGVRFFCSEKDGLPVRLIRGSSGPIKEMGFQKGNLQKESYKMSEGTVKWFNASKG
FGFIAQDNGNDVFVHFSAIKMEGYKALEEGARVRFDVVKGNKGPAADNVELL

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=70, Percent_Identity=62.8571428571429, Blast_Score=84, Evalue=4e-18,
Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=60, Blast_Score=80, Evalue=5e-17,
Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=58.4615384615385, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI2367114, Length=70, Percent_Identity=54.2857142857143, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=59.7014925373134, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=51.5625, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1787834, Length=70, Percent_Identity=54.2857142857143, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 14684; Mature: 14684

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAHVCAVPVCDIKIQNREPHNPHKEGVRFFCSEKDGLPVRLIRGSSGPIKEMGFQKGNL
CCCEEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHCCCCCCC
QKESYKMSEGTVKWFNASKGFGFIAQDNGNDVFVHFSAIKMEGYKALEEGARVRFDVVKG
CCCCEECCCCEEEEEECCCCCEEEEECCCCEEEEEEEEEEECCHHHHHCCCEEEEEEEEC
NKGPAADNVELL
CCCCCCCCEEEC
>Mature Secondary Structure
MRAHVCAVPVCDIKIQNREPHNPHKEGVRFFCSEKDGLPVRLIRGSSGPIKEMGFQKGNL
CCCEEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHCCCCCCC
QKESYKMSEGTVKWFNASKGFGFIAQDNGNDVFVHFSAIKMEGYKALEEGARVRFDVVKG
CCCCEECCCCEEEEEECCCCCEEEEECCCCEEEEEEEEEEECCHHHHHCCCEEEEEEEEC
NKGPAADNVELL
CCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]