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The map label for this gene is ygfK [H]

Identifier: 85860895

GI number: 85860895

Start: 3063459

End: 3065792

Strand: Direct

Name: ygfK [H]

Synonym: SYN_01629

Alternate gene names: 85860895

Gene position: 3063459-3065792 (Clockwise)

Preceding gene: 85860894

Following gene: 85860896

Centisome position: 96.36

GC content: 60.88

Gene sequence:

>2334_bases
ATGTCCTTTGAACCGACACAAATTCTCTCCGGAATGGAAAACGGGCTTCGCGTAGATTCCCGGATCCTCGAGGAGCGCGT
TCAGGCCGCGGTGCGCGACGGTCATCGGAGGATTGAAATTTCCGCCCATGGCCAGCACGGCATCGGCGGCCGCCTCTGGC
AGGCGGGCGAAGAAGAAATCCACCTCCGCGTTCGAGGCATGTCCGGTCAGCGTTTGGGGTCCATGGGTTTTCCCGGGACC
CGCATCGAGGTCTGCGGACCCGTTTCCGATGACGTCGGCTGGCTCAACGCGGGAGCCCGGATTATCGTCCACGGCCATGC
CACCAATGGAGCGGGAAACGCCATGGCCCAGGGAAAAATTTACATCGCCGGCAACATCGGCGCCCGGGGAATGACCATGA
CCAAGCACAATCCCCGCTTCGATCCCCCCGAGATGTGGATCCTAGGTTCCGCCGGCGATTCCTTCGCGGAATTCATGGCG
GGGGGAACAGCCGTAATCTGCGGGCATGATCCTCAGAACCCGTCCAATGTTCTGGGATATCGTCCCTGCGTGGGCATGGT
GGGCGGAAAGATTTACTTTCGCGGACTCCATCAGGGATTCAGCAAGCATAACGCCCGCCTGACGAATCTTACAGACGAGG
AATGGCAATGGCTGATTTCGGGATTGCGGGATTTTCTCTCCGAAATTGAGAGGATGAACCTTTTTGATCGCCTGACGGCG
GATCGGACCGCCTGGCAGCTTCTCGCCGCCCGGAAGCCCTATGAAAAAGTTGCCGTTGAATCCCGTCCCATAAACCGGTT
CCGCCGGGAAATCTGGGAAACCGAACTGGGTGCGGGCGGACTGATCGGGGATCTGACCGATCTGGACCGGAGTCCCATCG
ACGTGATTACGACGGGAGCCCTCCGCCGCTATGTTCCCCTGTGGGAAAATGGCCGCTATCTGCCCCCCTGTCAGGCAAAC
TGCCCGACAGGCATCCCGGTTCAGGAGCGCTGGCGGTTGATCCGGGAAGGGCATATTGCGGAAGCCGTCAATCTGTCCCT
GCGCTACACCCCTTTCCCGACGGCGGTCTGCGGCTATCTCTGTCCGAATCTCTGCATGGAGCACTGCACACGCCAGGCGG
AAAATCTTCCTCCCCTGGATGTCACGCTGCTGGGGAAAGCCAGCCTGCAGGCGGAAATGCCCCGGCCTGAGCCTTCCACG
AAACACCGGATTGCCGTGATCGGCGGCGGACCGGCGGGTCTTTCCGTGGCCTGGCAGCTCTGGATGAAGGGGCACGAGCC
GGTTGTCTATGAAAAAGAAGAAAAGCCGGGCGGCAAGATGACCGCCACGATTCCCCGCAGCCGCATACCTGACGAAGTTC
TGAACCATGAATTGCAGCGAATCCGGGAGGTGATCGAGTTCGTTTCTCTTGAGCAGCCCCTGACGGTGGAAAGATTCATG
CAGATTCGCGACTCTTCTGATTTTGTCGTGATTGCCGTGGGCGCTCAGAAACCAGCCACCCTGAATATTCCCGGCCATGA
ACGGGCCTTGACCGCCCTGGATTTCCTGCGGCTCTGCAAGCAGGACGGCATTCAGCCCGGTCGGCGGGTCGTCATTATCG
GCGCCGGCAACGTGGGGTGCGACGCGGCCACGGAGGCGGCCCGGCTGGGGGCAACGGACATCACCCTGATCGATATTCAG
GAACCGGCATCCTTTGGCCGGGAGCGGCAGCACGCCGAGGCCGCGGGGGCCCGTTTCCTCTGGCCGCGCTTTGCGAAGGC
CATTGCCAATGACGGCGTAGAGCTCGAAGATGGAGAATTCCTCCCGGCGGATACGGTGATTTTCGCCGTAGGGGATCGTC
CGGACCTGTCCTTCGTCCCGGACGAAATTATCCGGGAAAGGGGGTTCCTTCAGGTGGACGACCGTTTTCAGAGCAGCGCC
CCCCGGGTCTTTGCAGTGGGAGACGTCGTCCGTCCGGGATTGTTGACCGAGGCCATCGGGGCGGGTCGGATCGCAGCGGA
CACCATCGATGGACGAATTCGCGGCAGAAAAGAAACAATGGGCCAGTTGCAGCCGATGCCTGCTGAGCGGGTCAAGATGG
CCTATTTCGAACCCTGGCTGCCGGAAAACGCGGATGTGACAGCCTGGTCAAGAGTCTGCGCCTCCTGTGGCTCGTGCCGG
GATTGCGGACTCTGTGAAGCGATCTGTCCGCAGAATGCCATTTTCCGCCTGGATAAAAGCAGCGGGGCTTTTGAATACCG
GAGCCGGGCGGAGCGGTGCATCGGATGCGGTTTCTGCGCTGGGGTCTGTCCGACAGGCGTCTGGCGACTGGTGGAAAACG
AACCCCTGGAATAA

Upstream 100 bases:

>100_bases
CCCATCGGCATGTCCGACGCCCTGGGCATCGCGGACAACCATGCCGCCCGGCGGCTGGGGATTTCATATGTCATTTGAAA
AAAGATAAGAAGGTGCTTCC

Downstream 100 bases:

>100_bases
CATGATTTGATTCTTTGGAGAAAGGAGCCCTCTATGTTCAACTGTAAAACGAAAGAGGAAGTATTGCAGATCGTTAAAGA
ACAAAATGTCAGTTTCATTC

Product: glutamate synthase (NADPH)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 777; Mature: 776

Protein sequence:

>777_residues
MSFEPTQILSGMENGLRVDSRILEERVQAAVRDGHRRIEISAHGQHGIGGRLWQAGEEEIHLRVRGMSGQRLGSMGFPGT
RIEVCGPVSDDVGWLNAGARIIVHGHATNGAGNAMAQGKIYIAGNIGARGMTMTKHNPRFDPPEMWILGSAGDSFAEFMA
GGTAVICGHDPQNPSNVLGYRPCVGMVGGKIYFRGLHQGFSKHNARLTNLTDEEWQWLISGLRDFLSEIERMNLFDRLTA
DRTAWQLLAARKPYEKVAVESRPINRFRREIWETELGAGGLIGDLTDLDRSPIDVITTGALRRYVPLWENGRYLPPCQAN
CPTGIPVQERWRLIREGHIAEAVNLSLRYTPFPTAVCGYLCPNLCMEHCTRQAENLPPLDVTLLGKASLQAEMPRPEPST
KHRIAVIGGGPAGLSVAWQLWMKGHEPVVYEKEEKPGGKMTATIPRSRIPDEVLNHELQRIREVIEFVSLEQPLTVERFM
QIRDSSDFVVIAVGAQKPATLNIPGHERALTALDFLRLCKQDGIQPGRRVVIIGAGNVGCDAATEAARLGATDITLIDIQ
EPASFGRERQHAEAAGARFLWPRFAKAIANDGVELEDGEFLPADTVIFAVGDRPDLSFVPDEIIRERGFLQVDDRFQSSA
PRVFAVGDVVRPGLLTEAIGAGRIAADTIDGRIRGRKETMGQLQPMPAERVKMAYFEPWLPENADVTAWSRVCASCGSCR
DCGLCEAICPQNAIFRLDKSSGAFEYRSRAERCIGCGFCAGVCPTGVWRLVENEPLE

Sequences:

>Translated_777_residues
MSFEPTQILSGMENGLRVDSRILEERVQAAVRDGHRRIEISAHGQHGIGGRLWQAGEEEIHLRVRGMSGQRLGSMGFPGT
RIEVCGPVSDDVGWLNAGARIIVHGHATNGAGNAMAQGKIYIAGNIGARGMTMTKHNPRFDPPEMWILGSAGDSFAEFMA
GGTAVICGHDPQNPSNVLGYRPCVGMVGGKIYFRGLHQGFSKHNARLTNLTDEEWQWLISGLRDFLSEIERMNLFDRLTA
DRTAWQLLAARKPYEKVAVESRPINRFRREIWETELGAGGLIGDLTDLDRSPIDVITTGALRRYVPLWENGRYLPPCQAN
CPTGIPVQERWRLIREGHIAEAVNLSLRYTPFPTAVCGYLCPNLCMEHCTRQAENLPPLDVTLLGKASLQAEMPRPEPST
KHRIAVIGGGPAGLSVAWQLWMKGHEPVVYEKEEKPGGKMTATIPRSRIPDEVLNHELQRIREVIEFVSLEQPLTVERFM
QIRDSSDFVVIAVGAQKPATLNIPGHERALTALDFLRLCKQDGIQPGRRVVIIGAGNVGCDAATEAARLGATDITLIDIQ
EPASFGRERQHAEAAGARFLWPRFAKAIANDGVELEDGEFLPADTVIFAVGDRPDLSFVPDEIIRERGFLQVDDRFQSSA
PRVFAVGDVVRPGLLTEAIGAGRIAADTIDGRIRGRKETMGQLQPMPAERVKMAYFEPWLPENADVTAWSRVCASCGSCR
DCGLCEAICPQNAIFRLDKSSGAFEYRSRAERCIGCGFCAGVCPTGVWRLVENEPLE
>Mature_776_residues
SFEPTQILSGMENGLRVDSRILEERVQAAVRDGHRRIEISAHGQHGIGGRLWQAGEEEIHLRVRGMSGQRLGSMGFPGTR
IEVCGPVSDDVGWLNAGARIIVHGHATNGAGNAMAQGKIYIAGNIGARGMTMTKHNPRFDPPEMWILGSAGDSFAEFMAG
GTAVICGHDPQNPSNVLGYRPCVGMVGGKIYFRGLHQGFSKHNARLTNLTDEEWQWLISGLRDFLSEIERMNLFDRLTAD
RTAWQLLAARKPYEKVAVESRPINRFRREIWETELGAGGLIGDLTDLDRSPIDVITTGALRRYVPLWENGRYLPPCQANC
PTGIPVQERWRLIREGHIAEAVNLSLRYTPFPTAVCGYLCPNLCMEHCTRQAENLPPLDVTLLGKASLQAEMPRPEPSTK
HRIAVIGGGPAGLSVAWQLWMKGHEPVVYEKEEKPGGKMTATIPRSRIPDEVLNHELQRIREVIEFVSLEQPLTVERFMQ
IRDSSDFVVIAVGAQKPATLNIPGHERALTALDFLRLCKQDGIQPGRRVVIIGAGNVGCDAATEAARLGATDITLIDIQE
PASFGRERQHAEAAGARFLWPRFAKAIANDGVELEDGEFLPADTVIFAVGDRPDLSFVPDEIIRERGFLQVDDRFQSSAP
RVFAVGDVVRPGLLTEAIGAGRIAADTIDGRIRGRKETMGQLQPMPAERVKMAYFEPWLPENADVTAWSRVCASCGSCRD
CGLCEAICPQNAIFRLDKSSGAFEYRSRAERCIGCGFCAGVCPTGVWRLVENEPLE

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=431, Percent_Identity=24.1299303944316, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1789243, Length=500, Percent_Identity=27.4, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1788468, Length=406, Percent_Identity=28.0788177339901, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1789606, Length=422, Percent_Identity=28.6729857819905, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788811, Length=417, Percent_Identity=25.8992805755396, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI87082180, Length=424, Percent_Identity=26.6509433962264, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17570289, Length=445, Percent_Identity=23.3707865168539, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71984108, Length=416, Percent_Identity=26.6826923076923, Blast_Score=81, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=264, Percent_Identity=25, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24665547, Length=439, Percent_Identity=26.879271070615, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24665543, Length=439, Percent_Identity=26.879271070615, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28574881, Length=439, Percent_Identity=26.6514806378132, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24665539, Length=439, Percent_Identity=26.6514806378132, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640763, Length=424, Percent_Identity=26.1792452830189, Blast_Score=94, Evalue=5e-19,
Organism=Drosophila melanogaster, GI18858217, Length=424, Percent_Identity=26.1792452830189, Blast_Score=94, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR017701 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 85575; Mature: 85444

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFEPTQILSGMENGLRVDSRILEERVQAAVRDGHRRIEISAHGQHGIGGRLWQAGEEEI
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCEE
HLRVRGMSGQRLGSMGFPGTRIEVCGPVSDDVGWLNAGARIIVHGHATNGAGNAMAQGKI
EEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCEE
YIAGNIGARGMTMTKHNPRFDPPEMWILGSAGDSFAEFMAGGTAVICGHDPQNPSNVLGY
EEEECCCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHCCCEEEEECCCCCCCCCCCCC
RPCVGMVGGKIYFRGLHQGFSKHNARLTNLTDEEWQWLISGLRDFLSEIERMNLFDRLTA
CCCHHHHCCHHEEHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRTAWQLLAARKPYEKVAVESRPINRFRREIWETELGAGGLIGDLTDLDRSPIDVITTGA
HHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHEEHHH
LRRYVPLWENGRYLPPCQANCPTGIPVQERWRLIREGHIAEAVNLSLRYTPFPTAVCGYL
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHEEEEEEEECCCHHHHHHHH
CPNLCMEHCTRQAENLPPLDVTLLGKASLQAEMPRPEPSTKHRIAVIGGGPAGLSVAWQL
HHHHHHHHHHHHHCCCCCCCEEEECCCHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
WMKGHEPVVYEKEEKPGGKMTATIPRSRIPDEVLNHELQRIREVIEFVSLEQPLTVERFM
HHCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QIRDSSDFVVIAVGAQKPATLNIPGHERALTALDFLRLCKQDGIQPGRRVVIIGAGNVGC
HHCCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
DAATEAARLGATDITLIDIQEPASFGRERQHAEAAGARFLWPRFAKAIANDGVELEDGEF
CHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCCC
LPADTVIFAVGDRPDLSFVPDEIIRERGFLQVDDRFQSSAPRVFAVGDVVRPGLLTEAIG
CCCCEEEEEECCCCCCCCCCHHHHHHCCCEEECCHHHCCCCCEEEECCHHCCCHHHHHHC
AGRIAADTIDGRIRGRKETMGQLQPMPAERVKMAYFEPWLPENADVTAWSRVCASCGSCR
CCCEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
DCGLCEAICPQNAIFRLDKSSGAFEYRSRAERCIGCGFCAGVCPTGVWRLVENEPLE
CCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SFEPTQILSGMENGLRVDSRILEERVQAAVRDGHRRIEISAHGQHGIGGRLWQAGEEEI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCEE
HLRVRGMSGQRLGSMGFPGTRIEVCGPVSDDVGWLNAGARIIVHGHATNGAGNAMAQGKI
EEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCEE
YIAGNIGARGMTMTKHNPRFDPPEMWILGSAGDSFAEFMAGGTAVICGHDPQNPSNVLGY
EEEECCCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHCCCEEEEECCCCCCCCCCCCC
RPCVGMVGGKIYFRGLHQGFSKHNARLTNLTDEEWQWLISGLRDFLSEIERMNLFDRLTA
CCCHHHHCCHHEEHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRTAWQLLAARKPYEKVAVESRPINRFRREIWETELGAGGLIGDLTDLDRSPIDVITTGA
HHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHEEHHH
LRRYVPLWENGRYLPPCQANCPTGIPVQERWRLIREGHIAEAVNLSLRYTPFPTAVCGYL
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHEEEEEEEECCCHHHHHHHH
CPNLCMEHCTRQAENLPPLDVTLLGKASLQAEMPRPEPSTKHRIAVIGGGPAGLSVAWQL
HHHHHHHHHHHHHCCCCCCCEEEECCCHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
WMKGHEPVVYEKEEKPGGKMTATIPRSRIPDEVLNHELQRIREVIEFVSLEQPLTVERFM
HHCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QIRDSSDFVVIAVGAQKPATLNIPGHERALTALDFLRLCKQDGIQPGRRVVIIGAGNVGC
HHCCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
DAATEAARLGATDITLIDIQEPASFGRERQHAEAAGARFLWPRFAKAIANDGVELEDGEF
CHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCCC
LPADTVIFAVGDRPDLSFVPDEIIRERGFLQVDDRFQSSAPRVFAVGDVVRPGLLTEAIG
CCCCEEEEEECCCCCCCCCCHHHHHHCCCEEECCHHHCCCCCEEEECCHHCCCHHHHHHC
AGRIAADTIDGRIRGRKETMGQLQPMPAERVKMAYFEPWLPENADVTAWSRVCASCGSCR
CCCEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
DCGLCEAICPQNAIFRLDKSSGAFEYRSRAERCIGCGFCAGVCPTGVWRLVENEPLE
CCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]