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The map label for this gene is gltB [C]

Identifier: 85860894

GI number: 85860894

Start: 3061802

End: 3063436

Strand: Direct

Name: gltB [C]

Synonym: SYN_01630

Alternate gene names: 85860894

Gene position: 3061802-3063436 (Clockwise)

Preceding gene: 85860893

Following gene: 85860895

Centisome position: 96.3

GC content: 57.74

Gene sequence:

>1635_bases
ATGGAATCAATGGAAAAACTTACCCCTTCAGCATTAAATACAAAAGACCTTCCCTGGCTGATTCTCTGGGATAAGGAAAA
ATGCGCCTTATGCGGCCGGTGTACGGCCGTATGTCCGGTTCAGGCCATCGAGCCCGCTGTCTTCCGGAAGCGGGTTGTTT
CGGTGCAGCCGGGACTCGCCGAAAAACCGGGGCATCTGTATTCGATCTATCACGGAATCCGCCAGAAAACCGATCCGGCC
CATTTCTGCATCGGCTGCGGAATGTGCGATTTCGTCTGTCCCAATGGGGCTATCCAGCCGGTACGCAATGAAGAGCAGGA
TAAACTCCGATTTCATCTCAATTCAGGCGGAGCGCCCCGCCGTCGCGGTGGACGCCGCAATGTGCCGAGAAGTCTTCTCG
ATGAAATCAAATTTATCAGGATTTCCATGCTCACCGACCCGGCCCTCGATGCCGGACGACACGAATTTGAACTGCGAACG
CTGCTGGGCCGCATTCTTCCTCCCGAGGAGATTCTGAAGGTCTCCCGGGAAAACGGCTGGATTCCCCCCGTCCGGGAAAT
CTACCCCCTCGTGGTGGGCAGCATGTCCTTCGGGGCTCTTTCCCCCAACATGTGGGAGGGTCTCCAGATGGGTGTGGCCT
ATCTGAATGAGGAACTGGGCATGCCCGTGCGCATGGCCACCGGAGAAGGGGGCTGTCCGCCGCGTCTGCTCCGTTCGCGG
TTTCTGAAATACGTGATCCTTCAGATTGCAAGCGGCTATTTCGGCTGGGATGAAATTATCCGCACTCTGCCGGAAATGAA
GGAGGATCCCTGCGCCATCGAAATCAAATATGGACAGGGCGCAAAACCGGGCGATGGCGGACTGCTGATGTGGTATAAGG
TCAATAAACTGATTGCCGCGATTCGGGGTGTGCCGTCGGGCATCAATCTGCCCAGCCCCCCGACACACCAGACGCAGTAT
TCCATCGAAGAATCCGTGGCCAAGATGATTCAGTCCATGTCCATGGCCTGGGGATTCCGGGTTCCCGTCTATCCGAAGAT
CTCGGCGACGAGCACGGCCCTGGCGGTTCTCAACAATCTGACGCGCAATCCCTATGCTTCGGCACTGGCGATTGACGGCG
AAGACGGGGGCACCGGCGCGGCCTACCATGTTTCGATGAATTCCATGGGACACCCCATTGCCAGCAATATCCGGGATGCC
TACCTTACCCTGGTCAAACTCGGCAAACAGAACGAAATTCCTCTCTTTGCCGGCGGCGGCATCGGCAAGAACGGAAATCT
GGCGGCCAATGCCGCGGCCCTGATCATGCTGGGGGCCAGCGGTGTGCAGGTGGGCAAAGTCCTTATGCAGGCAGCGGCGG
GATGCGTCGGTTCAGAGACGGACCGGTGCAACATCTGTAATATCGGCCTCTGCCCGAAAGGGATCACCTCGCAGGATCCC
CGATTATACCGGCGGCTTGATCCGGAAAAAGTGGCGGAGCGTCTCGTGGATGTCTTTGTCAGCTTCGACACGGAATTGAA
AAAGATTGTCGCGCCGCTGGGACGGTCCACTTCCCTGCCCATCGGCATGTCCGACGCCCTGGGCATCGCGGACAACCATG
CCGCCCGGCGGCTGGGGATTTCATATGTCATTTGA

Upstream 100 bases:

>100_bases
GTCTTGATGAGGCCATTCCCGAACGGGATAAAAGTCGGGATTTTCAGCCCATGGGAACGGATCTGGTTTCTGTCGGACCG
GACCGGCGGGAGGTGAAACG

Downstream 100 bases:

>100_bases
AAAAAGATAAGAAGGTGCTTCCATGTCCTTTGAACCGACACAAATTCTCTCCGGAATGGAAAACGGGCTTCGCGTAGATT
CCCGGATCCTCGAGGAGCGC

Product: glutamate synthase (NADPH)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MESMEKLTPSALNTKDLPWLILWDKEKCALCGRCTAVCPVQAIEPAVFRKRVVSVQPGLAEKPGHLYSIYHGIRQKTDPA
HFCIGCGMCDFVCPNGAIQPVRNEEQDKLRFHLNSGGAPRRRGGRRNVPRSLLDEIKFIRISMLTDPALDAGRHEFELRT
LLGRILPPEEILKVSRENGWIPPVREIYPLVVGSMSFGALSPNMWEGLQMGVAYLNEELGMPVRMATGEGGCPPRLLRSR
FLKYVILQIASGYFGWDEIIRTLPEMKEDPCAIEIKYGQGAKPGDGGLLMWYKVNKLIAAIRGVPSGINLPSPPTHQTQY
SIEESVAKMIQSMSMAWGFRVPVYPKISATSTALAVLNNLTRNPYASALAIDGEDGGTGAAYHVSMNSMGHPIASNIRDA
YLTLVKLGKQNEIPLFAGGGIGKNGNLAANAAALIMLGASGVQVGKVLMQAAAGCVGSETDRCNICNIGLCPKGITSQDP
RLYRRLDPEKVAERLVDVFVSFDTELKKIVAPLGRSTSLPIGMSDALGIADNHAARRLGISYVI

Sequences:

>Translated_544_residues
MESMEKLTPSALNTKDLPWLILWDKEKCALCGRCTAVCPVQAIEPAVFRKRVVSVQPGLAEKPGHLYSIYHGIRQKTDPA
HFCIGCGMCDFVCPNGAIQPVRNEEQDKLRFHLNSGGAPRRRGGRRNVPRSLLDEIKFIRISMLTDPALDAGRHEFELRT
LLGRILPPEEILKVSRENGWIPPVREIYPLVVGSMSFGALSPNMWEGLQMGVAYLNEELGMPVRMATGEGGCPPRLLRSR
FLKYVILQIASGYFGWDEIIRTLPEMKEDPCAIEIKYGQGAKPGDGGLLMWYKVNKLIAAIRGVPSGINLPSPPTHQTQY
SIEESVAKMIQSMSMAWGFRVPVYPKISATSTALAVLNNLTRNPYASALAIDGEDGGTGAAYHVSMNSMGHPIASNIRDA
YLTLVKLGKQNEIPLFAGGGIGKNGNLAANAAALIMLGASGVQVGKVLMQAAAGCVGSETDRCNICNIGLCPKGITSQDP
RLYRRLDPEKVAERLVDVFVSFDTELKKIVAPLGRSTSLPIGMSDALGIADNHAARRLGISYVI
>Mature_544_residues
MESMEKLTPSALNTKDLPWLILWDKEKCALCGRCTAVCPVQAIEPAVFRKRVVSVQPGLAEKPGHLYSIYHGIRQKTDPA
HFCIGCGMCDFVCPNGAIQPVRNEEQDKLRFHLNSGGAPRRRGGRRNVPRSLLDEIKFIRISMLTDPALDAGRHEFELRT
LLGRILPPEEILKVSRENGWIPPVREIYPLVVGSMSFGALSPNMWEGLQMGVAYLNEELGMPVRMATGEGGCPPRLLRSR
FLKYVILQIASGYFGWDEIIRTLPEMKEDPCAIEIKYGQGAKPGDGGLLMWYKVNKLIAAIRGVPSGINLPSPPTHQTQY
SIEESVAKMIQSMSMAWGFRVPVYPKISATSTALAVLNNLTRNPYASALAIDGEDGGTGAAYHVSMNSMGHPIASNIRDA
YLTLVKLGKQNEIPLFAGGGIGKNGNLAANAAALIMLGASGVQVGKVLMQAAAGCVGSETDRCNICNIGLCPKGITSQDP
RLYRRLDPEKVAERLVDVFVSFDTELKKIVAPLGRSTSLPIGMSDALGIADNHAARRLGISYVI

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=387, Percent_Identity=28.6821705426357, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17570289, Length=359, Percent_Identity=27.5766016713092, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6320030, Length=385, Percent_Identity=26.7532467532467, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574881, Length=367, Percent_Identity=28.0653950953678, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24665539, Length=367, Percent_Identity=28.0653950953678, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 59177; Mature: 59177

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESMEKLTPSALNTKDLPWLILWDKEKCALCGRCTAVCPVQAIEPAVFRKRVVSVQPGLA
CCCHHHCCCCCCCCCCCCEEEEECCCHHHHHCCCHHCCCHHHCCHHHHHHHHHHCCCCCC
EKPGHLYSIYHGIRQKTDPAHFCIGCGMCDFVCPNGAIQPVRNEEQDKLRFHLNSGGAPR
CCCCHHHHHHHHHHCCCCCCCEEEECCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCC
RRGGRRNVPRSLLDEIKFIRISMLTDPALDAGRHEFELRTLLGRILPPEEILKVSRENGW
CCCCCCCCCHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCC
IPPVREIYPLVVGSMSFGALSPNMWEGLQMGVAYLNEELGMPVRMATGEGGCPPRLLRSR
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
FLKYVILQIASGYFGWDEIIRTLPEMKEDPCAIEIKYGQGAKPGDGGLLMWYKVNKLIAA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEHHHHHHHH
IRGVPSGINLPSPPTHQTQYSIEESVAKMIQSMSMAWGFRVPVYPKISATSTALAVLNNL
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
TRNPYASALAIDGEDGGTGAAYHVSMNSMGHPIASNIRDAYLTLVKLGKQNEIPLFAGGG
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCC
IGKNGNLAANAAALIMLGASGVQVGKVLMQAAAGCVGSETDRCNICNIGLCPKGITSQDP
CCCCCCCHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCC
RLYRRLDPEKVAERLVDVFVSFDTELKKIVAPLGRSTSLPIGMSDALGIADNHAARRLGI
HHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCC
SYVI
EECC
>Mature Secondary Structure
MESMEKLTPSALNTKDLPWLILWDKEKCALCGRCTAVCPVQAIEPAVFRKRVVSVQPGLA
CCCHHHCCCCCCCCCCCCEEEEECCCHHHHHCCCHHCCCHHHCCHHHHHHHHHHCCCCCC
EKPGHLYSIYHGIRQKTDPAHFCIGCGMCDFVCPNGAIQPVRNEEQDKLRFHLNSGGAPR
CCCCHHHHHHHHHHCCCCCCCEEEECCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCC
RRGGRRNVPRSLLDEIKFIRISMLTDPALDAGRHEFELRTLLGRILPPEEILKVSRENGW
CCCCCCCCCHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCC
IPPVREIYPLVVGSMSFGALSPNMWEGLQMGVAYLNEELGMPVRMATGEGGCPPRLLRSR
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
FLKYVILQIASGYFGWDEIIRTLPEMKEDPCAIEIKYGQGAKPGDGGLLMWYKVNKLIAA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEHHHHHHHH
IRGVPSGINLPSPPTHQTQYSIEESVAKMIQSMSMAWGFRVPVYPKISATSTALAVLNNL
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
TRNPYASALAIDGEDGGTGAAYHVSMNSMGHPIASNIRDAYLTLVKLGKQNEIPLFAGGG
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCC
IGKNGNLAANAAALIMLGASGVQVGKVLMQAAAGCVGSETDRCNICNIGLCPKGITSQDP
CCCCCCCHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCC
RLYRRLDPEKVAERLVDVFVSFDTELKKIVAPLGRSTSLPIGMSDALGIADNHAARRLGI
HHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCC
SYVI
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]