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The map label for this gene is vnfA [H]

Identifier: 85860891

GI number: 85860891

Start: 3058831

End: 3060372

Strand: Direct

Name: vnfA [H]

Synonym: SYN_01633

Alternate gene names: 85860891

Gene position: 3058831-3060372 (Clockwise)

Preceding gene: 85860890

Following gene: 85860893

Centisome position: 96.21

GC content: 56.29

Gene sequence:

>1542_bases
TTGAGCAAACATAATTATGAGGAACTGGCGGCATTGCATGCCATTGCAAGGACTCTGGCTCAGCCCGGGGAACTGCGGGA
TCAACTGGAGCGGGTTCTTCAGGAGATGAACCAGAGGGTCGGAATGCAACGGGGAATGATCTCTCTGCTGGACAGGGAAA
GCGGTGAAGTCTGGCTGGATGTGGCTCACGGGGTTGACATCCAGGGGATGGAAGTCTCCTACCAGCCTGGAGAGGGGATT
ACCGGCAAGGTCGCTCAGACGGGACGCCCCATGGCCGTCGCCAATCTGGGGCAGGAAACCCATTTTCTCGACCGGACCGG
AGCGCGGCGATTTCTCAATCGCTCGGAACTGGCCTTTCTCTGTGTGCCGATTATTTATGACAATCGGGTGGTCGGGGTCC
TGTCCGCCGATAAGGCCGCCCGCCAGGTGGAGGACCTGGATCGGGAACTGGCCATGCTTTCCTCCATATCCGAACTCATT
GCCAAGAGCGTTCACATCCGCGCCCTGGAAGAGGAAAACCGGCGCCTCCGGCATATGCTTGGCCGCTCGACTGTGCCGGA
TTCCGAAATCATCGGTCATTCCAGGGTCATGCAGGAAGTCTTCGGCCTCATTGCCCAGGTGGCCGATTCCAATACGACCG
TGCTCATCAACGGCGAAACGGGGACAGGAAAGGAGCTGGTTGCCCGGGCGATCCACGACCGCAGCTCCCGGTGCAAGGGG
CCTCTGATTCAGGTGAATTGCGCCGCCATGCCGGATACCTTGATCGAAAGCGAACTTTTCGGCCACGAAAAAGGGGCGTT
TACGGGAGCGCTTCACGCGCGTCGGGGACGTTTCGAGGAGGCTCATGGCGGTACCATTTTCCTCGATGAGGTCGGCGAGC
TGTCCGCGGCGGCCCAGGCAAAACTGCTGAGGGTCATTCAGGAGAAGAAGTTTCAGCCTCTAGGCGCTTCGCGCGTGGTC
TCCGTAGATGTCCGCATTATCGCGGCAACCAACCGGAATCTGGAAGAGGATGTGGCAGTGGGTCACTTCCGGGCCGATCT
GTATTACCGGTTGAATGTCTTTCCGATTTACCTCCCGCCGCTTCGGGAGCGGGGAAGCGACATCATCCTCCTGGCCGATT
ATTTTGTATTGAAGTATAATAAAATAATGGGGAAATCCGTAAAGCGGATTTCGAATGCCGTTCTGGAAGCCTTTTTAAGC
CATTCCTGGCCGGGCAACGTCCGGGAACTTGAAAACTGCATAGAACGGGCGGTTCTGCTGGCAACAGGGGATTCCATCGA
AACACTCCATCTGCCTCCCTCACTCCAGATCAGGAGCCGGGAAGGAGAGAAGAAGGAATCAGGCAAATTTAATGCGATGA
TCGAATCCCAGGAAAGGGCGATGATTGTGGACGCACTCAAGGAAAGTCATGGCAACCAAAGCCAGGCAGCACGAATTCTC
GGGACAACAAAACGCATCATTCAATACAAGATCCAGAAATTCGGGATCGATCCCCGTCGGTTTAACCCCAAACATGCCGG
TTCATCCACGATTTCCGGCTGA

Upstream 100 bases:

>100_bases
CCCAGGAAGGGAACGGAACGGCCGGTGGATGAGATCGCGGGTTTCTTGAAAAGGGGGACAAAGGCAGTCGGAACGACGAG
GTAACGAAGGTGGTCCGTAA

Downstream 100 bases:

>100_bases
ATTGAGATGGGCCGGGAAAAGGTCACGGCTTTTCTTGAATTTTGATACAAATTTGTTCTTTATTTGGTCATTTGCACATT
TTTGTTTCTTTTCATTTTCA

Product: nif-specific regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSKHNYEELAALHAIARTLAQPGELRDQLERVLQEMNQRVGMQRGMISLLDRESGEVWLDVAHGVDIQGMEVSYQPGEGI
TGKVAQTGRPMAVANLGQETHFLDRTGARRFLNRSELAFLCVPIIYDNRVVGVLSADKAARQVEDLDRELAMLSSISELI
AKSVHIRALEEENRRLRHMLGRSTVPDSEIIGHSRVMQEVFGLIAQVADSNTTVLINGETGTGKELVARAIHDRSSRCKG
PLIQVNCAAMPDTLIESELFGHEKGAFTGALHARRGRFEEAHGGTIFLDEVGELSAAAQAKLLRVIQEKKFQPLGASRVV
SVDVRIIAATNRNLEEDVAVGHFRADLYYRLNVFPIYLPPLRERGSDIILLADYFVLKYNKIMGKSVKRISNAVLEAFLS
HSWPGNVRELENCIERAVLLATGDSIETLHLPPSLQIRSREGEKKESGKFNAMIESQERAMIVDALKESHGNQSQAARIL
GTTKRIIQYKIQKFGIDPRRFNPKHAGSSTISG

Sequences:

>Translated_513_residues
MSKHNYEELAALHAIARTLAQPGELRDQLERVLQEMNQRVGMQRGMISLLDRESGEVWLDVAHGVDIQGMEVSYQPGEGI
TGKVAQTGRPMAVANLGQETHFLDRTGARRFLNRSELAFLCVPIIYDNRVVGVLSADKAARQVEDLDRELAMLSSISELI
AKSVHIRALEEENRRLRHMLGRSTVPDSEIIGHSRVMQEVFGLIAQVADSNTTVLINGETGTGKELVARAIHDRSSRCKG
PLIQVNCAAMPDTLIESELFGHEKGAFTGALHARRGRFEEAHGGTIFLDEVGELSAAAQAKLLRVIQEKKFQPLGASRVV
SVDVRIIAATNRNLEEDVAVGHFRADLYYRLNVFPIYLPPLRERGSDIILLADYFVLKYNKIMGKSVKRISNAVLEAFLS
HSWPGNVRELENCIERAVLLATGDSIETLHLPPSLQIRSREGEKKESGKFNAMIESQERAMIVDALKESHGNQSQAARIL
GTTKRIIQYKIQKFGIDPRRFNPKHAGSSTISG
>Mature_512_residues
SKHNYEELAALHAIARTLAQPGELRDQLERVLQEMNQRVGMQRGMISLLDRESGEVWLDVAHGVDIQGMEVSYQPGEGIT
GKVAQTGRPMAVANLGQETHFLDRTGARRFLNRSELAFLCVPIIYDNRVVGVLSADKAARQVEDLDRELAMLSSISELIA
KSVHIRALEEENRRLRHMLGRSTVPDSEIIGHSRVMQEVFGLIAQVADSNTTVLINGETGTGKELVARAIHDRSSRCKGP
LIQVNCAAMPDTLIESELFGHEKGAFTGALHARRGRFEEAHGGTIFLDEVGELSAAAQAKLLRVIQEKKFQPLGASRVVS
VDVRIIAATNRNLEEDVAVGHFRADLYYRLNVFPIYLPPLRERGSDIILLADYFVLKYNKIMGKSVKRISNAVLEAFLSH
SWPGNVRELENCIERAVLLATGDSIETLHLPPSLQIRSREGEKKESGKFNAMIESQERAMIVDALKESHGNQSQAARILG
TTKRIIQYKIQKFGIDPRRFNPKHAGSSTISG

Specific function: Required for the expression of the V-dependent nitrogen fixation system in Azotobacter vinelandii. It is required for the regulation of nitrogenase 2 transcription. Interacts with sigma- 54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=351, Percent_Identity=44.4444444444444, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1789087, Length=398, Percent_Identity=43.7185929648241, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1790437, Length=304, Percent_Identity=48.6842105263158, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI87082117, Length=344, Percent_Identity=46.8023255813954, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1790299, Length=335, Percent_Identity=42.9850746268657, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI87082152, Length=340, Percent_Identity=45.5882352941176, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=42.9467084639498, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1788905, Length=312, Percent_Identity=41.025641025641, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1786524, Length=333, Percent_Identity=39.039039039039, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI87081872, Length=334, Percent_Identity=38.3233532934132, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1787583, Length=344, Percent_Identity=34.3023255813954, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=42.4778761061947, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=31.986531986532, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56962; Mature: 56831

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHNYEELAALHAIARTLAQPGELRDQLERVLQEMNQRVGMQRGMISLLDRESGEVWLD
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VAHGVDIQGMEVSYQPGEGITGKVAQTGRPMAVANLGQETHFLDRTGARRFLNRSELAFL
EECCCCCCCCEEEECCCCCCCCCHHCCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCEEEE
CVPIIYDNRVVGVLSADKAARQVEDLDRELAMLSSISELIAKSVHIRALEEENRRLRHML
EEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRSTVPDSEIIGHSRVMQEVFGLIAQVADSNTTVLINGETGTGKELVARAIHDRSSRCKG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
PLIQVNCAAMPDTLIESELFGHEKGAFTGALHARRGRFEEAHGGTIFLDEVGELSAAAQA
CEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHH
KLLRVIQEKKFQPLGASRVVSVDVRIIAATNRNLEEDVAVGHFRADLYYRLNVFPIYLPP
HHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHEEEEEEEEEEEEEEECCC
LRERGSDIILLADYFVLKYNKIMGKSVKRISNAVLEAFLSHSWPGNVRELENCIERAVLL
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEE
ATGDSIETLHLPPSLQIRSREGEKKESGKFNAMIESQERAMIVDALKESHGNQSQAARIL
EECCCCEEEECCCCCEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHH
GTTKRIIQYKIQKFGIDPRRFNPKHAGSSTISG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKHNYEELAALHAIARTLAQPGELRDQLERVLQEMNQRVGMQRGMISLLDRESGEVWLD
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VAHGVDIQGMEVSYQPGEGITGKVAQTGRPMAVANLGQETHFLDRTGARRFLNRSELAFL
EECCCCCCCCEEEECCCCCCCCCHHCCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCEEEE
CVPIIYDNRVVGVLSADKAARQVEDLDRELAMLSSISELIAKSVHIRALEEENRRLRHML
EEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRSTVPDSEIIGHSRVMQEVFGLIAQVADSNTTVLINGETGTGKELVARAIHDRSSRCKG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
PLIQVNCAAMPDTLIESELFGHEKGAFTGALHARRGRFEEAHGGTIFLDEVGELSAAAQA
CEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHH
KLLRVIQEKKFQPLGASRVVSVDVRIIAATNRNLEEDVAVGHFRADLYYRLNVFPIYLPP
HHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHEEEEEEEEEEEEEEECCC
LRERGSDIILLADYFVLKYNKIMGKSVKRISNAVLEAFLSHSWPGNVRELENCIERAVLL
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEE
ATGDSIETLHLPPSLQIRSREGEKKESGKFNAMIESQERAMIVDALKESHGNQSQAARIL
EECCCCEEEECCCCCEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHH
GTTKRIIQYKIQKFGIDPRRFNPKHAGSSTISG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]