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The map label for this gene is rnfC [H]

Identifier: 85860862

GI number: 85860862

Start: 3023605

End: 3024924

Strand: Direct

Name: rnfC [H]

Synonym: SYN_01664

Alternate gene names: 85860862

Gene position: 3023605-3024924 (Clockwise)

Preceding gene: 85860861

Following gene: 85860863

Centisome position: 95.1

GC content: 53.11

Gene sequence:

>1320_bases
ATGACTGCATCATTGTTGACTTTCGCTAAAGGCGGAGTTCATCCTCCAGAGTCCAAGGAGATTACTGAACATCTGGCTAT
TGAAAAGATGCCCTTGCCGGCACAGGTTGAAATTCCGCTTCTTCAGCATTTTGGCGCTCCCTGCCAGCCCCTGGTGAACA
AAAAGGACCGTGTGGAAGAAGGCGACCTGATCGGTCAGGTTCAGGGTCTGGGAGCAAATGTTCACGCCAGCATTACCGGC
ACGGTGACGAATGTCGGCACATCCATCGGACCGACCTCGCTCAACATTCCCTCCGTGACCATTGATCGGGATACTGAAGC
GCCTGATAAAATCTACACTCCCTCGGACTGGAGAGGTCTGTCCCCTCAGGAACTGCTTCAGAAGATCAAGGATGGCGGCA
TCGTCGGGATTGGCGGTGCAGGTTTCCCGGCTCATGTCAAGCTGTCTCCACCGCCGGATGCCAAGGTTGACACCCTGGTT
TTGAACGGCGCAGAATGTGAACCCTACCTGACTTGCGATCATCGCGTCATGCTCGAGAAACCTGAAGAGGTGGTTGAAGG
CGCAAAGATTCTTCTGCATGTTCTGGGCATCAAAGATTGTTATATCGGTGTTGAATCGAATAAAAATGACGCCATCGACG
CTCTTAGGAAAACCATTGAACAGCAGAAGCTTGACGGGGTAAAGATTCAGGTCAGCCCCCTGAAAGTCAAGTATCCTCAA
GGTTCCGAGAAACAATTGATCGAAAGCACCACCGGCAGAAAGGTGCCTGGCGGCGGATTGCCTTTCGATGTCGGCGTCAT
TGTGCAGAACGTCGGTACGGCCAAGGCGGTATTTGACGCGGTGGTTCTCAATAAACCGCTCTATGAAAAGGTCATCACCA
TATCCGGCAGAGGCATTTCCAGGCCCGCCAATCTACAGGTTCGGATCGGAACGCGCATTCGGGACATTGTTGAGTACCTG
GGAGGCACCAAGGCTGATCTGGCCAAGATCGTCATGGGAGGCCCCATGATGGGATTCGCCGTTTCGAGCTTGGACATTCC
AGTAACAAAAACCTGTTCCGGAATTCTCTTTCTGACTCGTGATGAAATTGATGAACAGCCCTATGGACCCTGCATACGGT
GCGGATGGTGCGTCGAAGCCTGCCCCATGGGGCTCTCTCCCAATGAGATCGGCCTGTACGTAGAAGCCGGTCGGGCTGCC
GACACCGCACCTTTCGGTGTGTTTGATTGTTTTGAGTGCGGTTCTTGCGCCTTTGTATGTCCAGCCAAGAGGCCGCTTGT
CCAATTTATCCGCCTGGCCAAGCAAAAGGCGAGAAAATAG

Upstream 100 bases:

>100_bases
CACATTGCGGCTACGGTAGACAGGCTAATGATCCGAAACATAGTGACCGGATCGTTAGGCTTGAAAAAGAAACTTTAATG
AAATCATCGGGTTATTAACT

Downstream 100 bases:

>100_bases
TTAAAAAGGAGTCAAGCCATCGTGGAGCAAGTAAAAGACACTCAGATTTCAGAAAATCCATTGCCGGCATTGACGGTCTC
CCTGTCGCCCCATGTCAAGA

Product: electron transport complex protein, NADH-binding subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTASLLTFAKGGVHPPESKEITEHLAIEKMPLPAQVEIPLLQHFGAPCQPLVNKKDRVEEGDLIGQVQGLGANVHASITG
TVTNVGTSIGPTSLNIPSVTIDRDTEAPDKIYTPSDWRGLSPQELLQKIKDGGIVGIGGAGFPAHVKLSPPPDAKVDTLV
LNGAECEPYLTCDHRVMLEKPEEVVEGAKILLHVLGIKDCYIGVESNKNDAIDALRKTIEQQKLDGVKIQVSPLKVKYPQ
GSEKQLIESTTGRKVPGGGLPFDVGVIVQNVGTAKAVFDAVVLNKPLYEKVITISGRGISRPANLQVRIGTRIRDIVEYL
GGTKADLAKIVMGGPMMGFAVSSLDIPVTKTCSGILFLTRDEIDEQPYGPCIRCGWCVEACPMGLSPNEIGLYVEAGRAA
DTAPFGVFDCFECGSCAFVCPAKRPLVQFIRLAKQKARK

Sequences:

>Translated_439_residues
MTASLLTFAKGGVHPPESKEITEHLAIEKMPLPAQVEIPLLQHFGAPCQPLVNKKDRVEEGDLIGQVQGLGANVHASITG
TVTNVGTSIGPTSLNIPSVTIDRDTEAPDKIYTPSDWRGLSPQELLQKIKDGGIVGIGGAGFPAHVKLSPPPDAKVDTLV
LNGAECEPYLTCDHRVMLEKPEEVVEGAKILLHVLGIKDCYIGVESNKNDAIDALRKTIEQQKLDGVKIQVSPLKVKYPQ
GSEKQLIESTTGRKVPGGGLPFDVGVIVQNVGTAKAVFDAVVLNKPLYEKVITISGRGISRPANLQVRIGTRIRDIVEYL
GGTKADLAKIVMGGPMMGFAVSSLDIPVTKTCSGILFLTRDEIDEQPYGPCIRCGWCVEACPMGLSPNEIGLYVEAGRAA
DTAPFGVFDCFECGSCAFVCPAKRPLVQFIRLAKQKARK
>Mature_438_residues
TASLLTFAKGGVHPPESKEITEHLAIEKMPLPAQVEIPLLQHFGAPCQPLVNKKDRVEEGDLIGQVQGLGANVHASITGT
VTNVGTSIGPTSLNIPSVTIDRDTEAPDKIYTPSDWRGLSPQELLQKIKDGGIVGIGGAGFPAHVKLSPPPDAKVDTLVL
NGAECEPYLTCDHRVMLEKPEEVVEGAKILLHVLGIKDCYIGVESNKNDAIDALRKTIEQQKLDGVKIQVSPLKVKYPQG
SEKQLIESTTGRKVPGGGLPFDVGVIVQNVGTAKAVFDAVVLNKPLYEKVITISGRGISRPANLQVRIGTRIRDIVEYLG
GTKADLAKIVMGGPMMGFAVSSLDIPVTKTCSGILFLTRDEIDEQPYGPCIRCGWCVEACPMGLSPNEIGLYVEAGRAAD
TAPFGVFDCFECGSCAFVCPAKRPLVQFIRLAKQKARK

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=431, Percent_Identity=39.4431554524362, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1788620, Length=267, Percent_Identity=28.0898876404494, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 47020; Mature: 46889

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASLLTFAKGGVHPPESKEITEHLAIEKMPLPAQVEIPLLQHFGAPCQPLVNKKDRVEE
CCCCCEEHHCCCCCCCCCHHHHHHHHHHCCCCCCEEECHHHHHCCCCCHHHCCCCCCCCC
GDLIGQVQGLGANVHASITGTVTNVGTSIGPTSLNIPSVTIDRDTEAPDKIYTPSDWRGL
CCCEEEEECCCCEEEEEEEEEHEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
SPQELLQKIKDGGIVGIGGAGFPAHVKLSPPPDAKVDTLVLNGAECEPYLTCDHRVMLEK
CHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCCCEEEEEEECCCCCCEEECCCEEEECC
PEEVVEGAKILLHVLGIKDCYIGVESNKNDAIDALRKTIEQQKLDGVKIQVSPLKVKYPQ
CHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCC
GSEKQLIESTTGRKVPGGGLPFDVGVIVQNVGTAKAVFDAVVLNKPLYEKVITISGRGIS
CCHHHHHHHCCCCCCCCCCCCEEHHHHHHCCCHHHHHHHHHHHCCCHHHEEEEEECCCCC
RPANLQVRIGTRIRDIVEYLGGTKADLAKIVMGGPMMGFAVSSLDIPVTKTCSGILFLTR
CCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHCCCCHHHEECCCCCCHHHCCCCEEEEEC
DEIDEQPYGPCIRCGWCVEACPMGLSPNEIGLYVEAGRAADTAPFGVFDCFECGSCAFVC
CCCCCCCCCCEEEECCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCEEEEC
PAKRPLVQFIRLAKQKARK
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TASLLTFAKGGVHPPESKEITEHLAIEKMPLPAQVEIPLLQHFGAPCQPLVNKKDRVEE
CCCCEEHHCCCCCCCCCHHHHHHHHHHCCCCCCEEECHHHHHCCCCCHHHCCCCCCCCC
GDLIGQVQGLGANVHASITGTVTNVGTSIGPTSLNIPSVTIDRDTEAPDKIYTPSDWRGL
CCCEEEEECCCCEEEEEEEEEHEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
SPQELLQKIKDGGIVGIGGAGFPAHVKLSPPPDAKVDTLVLNGAECEPYLTCDHRVMLEK
CHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCCCEEEEEEECCCCCCEEECCCEEEECC
PEEVVEGAKILLHVLGIKDCYIGVESNKNDAIDALRKTIEQQKLDGVKIQVSPLKVKYPQ
CHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCC
GSEKQLIESTTGRKVPGGGLPFDVGVIVQNVGTAKAVFDAVVLNKPLYEKVITISGRGIS
CCHHHHHHHCCCCCCCCCCCCEEHHHHHHCCCHHHHHHHHHHHCCCHHHEEEEEECCCCC
RPANLQVRIGTRIRDIVEYLGGTKADLAKIVMGGPMMGFAVSSLDIPVTKTCSGILFLTR
CCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHCCCCHHHEECCCCCCHHHCCCCEEEEEC
DEIDEQPYGPCIRCGWCVEACPMGLSPNEIGLYVEAGRAADTAPFGVFDCFECGSCAFVC
CCCCCCCCCCEEEECCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCEEEEC
PAKRPLVQFIRLAKQKARK
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]