Click here to switch to the map view.

The map label for this gene is pbpG [H]

Identifier: 85860813

GI number: 85860813

Start: 2978134

End: 2980158

Strand: Direct

Name: pbpG [H]

Synonym: SYN_00304

Alternate gene names: 85860813

Gene position: 2978134-2980158 (Clockwise)

Preceding gene: 85860812

Following gene: 85860815

Centisome position: 93.67

GC content: 56.1

Gene sequence:

>2025_bases
ATGGTCAAAACTTCTCAACATCCAGTGACAAGAAACCGGTGTTCTGCCGGCAGTATTCTGATCGTGCTTGTTTTCTTTCT
CCTGCTGGCCCTGCTGGTGGGCAGCAGCATCCTCTATTATGTCGTTCTTCGGGATCTGCCCAGCATTGCCGCATTGAAGG
AATACCGGCCAAGCATCACCACCCGGGTTTACGCGGACAATAATGAACTGATCGACGAATTCTACATGGAAAATCGTAAG
CTGGTCAGATTCGAGGAAATCCCATCCCTGGTCATCAAAGCCTTTCTGGCCGCTGAGGATGCCCGGTTTTTTGAACACCG
CGGTTTTGACGTGCAGAGCATTTCCCGGGCCTTTTTCAGAAATCTCAGGGCCGGAAGGATTGTCCAGGGGGGCAGCACCA
TTACGCAACAGGTCGCCAAGGCCCTCTATCTGTCCTCGGAAAAAAGTTACAGCCGCAAAATCAAGGAAGCCGTCCTGGCC
TATAAGATCGACCGTTCCCTTTCGAAAAAGGAAATTCTGAATCTCTATCTCAATCATATTTATCTTGGCCACGGCGCTTA
TGGGATTGAAGCGGCCTCTCAGGTTTATTTCGGAAAAAGAACGCGGGATCTTAAACTTTCCGAAGCCTCTCTCCTGGCCG
GACTGCCCAAGGCGCCCTCCAGTTATGATCCTTATACCAGTTATCAGCGGGCCCGGCAGCGTCAAACCTACGTCCTGAGC
CGGATGGTGGAGGATGGCCACATCACCGAGGAAGAGAAGAACCATGTGCTGCAGACGCCTCTGCCCCTAAGATCGATCCG
GCCCAGAGACCGGGTTGCCGCCTACTTTATCGAAAATATCCGCCGTTACATCCAGGGAAAGTACGGCAGTGACGCCTTGT
ATAAGGAGGGCCTTGAAGTATATACCACGCTGGACATCCGCACTCAGCAGGCAGCACGGGACGCCGTCGAACGGGGGCTC
AAGGAACTGGAAAGTCGGCAGGGCTACTCAAGGGATATGGTTCAGGGCGCCCTCCTTTGCATGGACGCCCGCACAGGGGC
CATCCGGGCCATGGTCGGCGGCCGGGACTTCAGCAAAAGCGAATTCAACCGTGCCACTCAGTCGCGAAGGCAGCCGGGTT
CCGCCTTCAAACCCATTATCTATGCGGCGGCTTTCGATCGGGGAATGACCCCGGCCACGGAGATTGTCGATGCTCCGCTT
GCTTTCGAGGATGGGATGGGACATGCCTGGACACCTCAAAACTATGACGAGGAATTTCTGGGACCGATCTCCCTGCGCAC
GGCCCTCGTTCAATCCCGCAACGTTGTCACGATCAAACTGCTGCAGGAAATCGGTGTGGATTACGCCATTGCCTATGCCG
CCAACATGGGGATTTCTTCACGGCTGGCCCGGGACCTGTCGCTTGCCCTGGGTTCCTCGGCGGTCACCCTGCAGGAGCTG
GTACGGTCCTATGCCGTCCTGGCCAATGGAGGGAAAATCGTGGAACCGTTCATGATCAAGAAGATCGTGGATCGGACGGG
CTATGTCTTTGAAGAGCACACAATTAAAGTGGAGCAGGCCATCGATCCCCGCATCGCCTTTCTGACGACTTCCATTCTGC
AGGATGCTGTGGAAAGCGGGACGGGGCAGCGCGTTAAAAGTATCGGCCGGCCTGTAGCAGGAAAAACAGGAACAACCAAC
GACGTGCATGATGCCTGGTTTGTCGGTTTCACCCCCTCTCTTGTCACCGGCGTCTGGGTCGGCTTTGACCAGCAGCGCCC
TCTGGGCAACCAGGAAGTCGGTGGCCTGGCCGCTGCGCCGATCTGGCTCTACTGTATGGAAAGGGCTTTAGCCAACAAGC
CGGTGGAGAGTTTTCCCGTTCCGGATGGAATTGTCTTTGTCCGGCGAAACGAAGGCGCGCTTCCCGAGCCTTTTATTGAC
GGGACCGCCCCTCCCGACGCGATCCCCGCCGACGCGGAGGGAAGCACCTCAACGGAGGAGGAGGGTGAAGAGGATCCCGG
CTGGAAGTTCAGATTTCCTTTTTAG

Upstream 100 bases:

>100_bases
GCGGGGCGCAAGCGTCGCGGCACTCTGGAGGAAACCGGCAACGAGATCGACCGAAAAGAAGAAGAGGAAGACATCCCGGA
AACGCCCAAGGCTGAATAAG

Downstream 100 bases:

>100_bases
GAGTTAAAAAACGACAAGGAAGTGCTTATTTATTTCAAGAATTTTCTTGACAAGGGTTTTCTTTTTCGATAGAAAATACT
GATAGTTTTTTGTGTGAACA

Product: multimodular transpeptidase-transglycosylase

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MVKTSQHPVTRNRCSAGSILIVLVFFLLLALLVGSSILYYVVLRDLPSIAALKEYRPSITTRVYADNNELIDEFYMENRK
LVRFEEIPSLVIKAFLAAEDARFFEHRGFDVQSISRAFFRNLRAGRIVQGGSTITQQVAKALYLSSEKSYSRKIKEAVLA
YKIDRSLSKKEILNLYLNHIYLGHGAYGIEAASQVYFGKRTRDLKLSEASLLAGLPKAPSSYDPYTSYQRARQRQTYVLS
RMVEDGHITEEEKNHVLQTPLPLRSIRPRDRVAAYFIENIRRYIQGKYGSDALYKEGLEVYTTLDIRTQQAARDAVERGL
KELESRQGYSRDMVQGALLCMDARTGAIRAMVGGRDFSKSEFNRATQSRRQPGSAFKPIIYAAAFDRGMTPATEIVDAPL
AFEDGMGHAWTPQNYDEEFLGPISLRTALVQSRNVVTIKLLQEIGVDYAIAYAANMGISSRLARDLSLALGSSAVTLQEL
VRSYAVLANGGKIVEPFMIKKIVDRTGYVFEEHTIKVEQAIDPRIAFLTTSILQDAVESGTGQRVKSIGRPVAGKTGTTN
DVHDAWFVGFTPSLVTGVWVGFDQQRPLGNQEVGGLAAAPIWLYCMERALANKPVESFPVPDGIVFVRRNEGALPEPFID
GTAPPDAIPADAEGSTSTEEEGEEDPGWKFRFPF

Sequences:

>Translated_674_residues
MVKTSQHPVTRNRCSAGSILIVLVFFLLLALLVGSSILYYVVLRDLPSIAALKEYRPSITTRVYADNNELIDEFYMENRK
LVRFEEIPSLVIKAFLAAEDARFFEHRGFDVQSISRAFFRNLRAGRIVQGGSTITQQVAKALYLSSEKSYSRKIKEAVLA
YKIDRSLSKKEILNLYLNHIYLGHGAYGIEAASQVYFGKRTRDLKLSEASLLAGLPKAPSSYDPYTSYQRARQRQTYVLS
RMVEDGHITEEEKNHVLQTPLPLRSIRPRDRVAAYFIENIRRYIQGKYGSDALYKEGLEVYTTLDIRTQQAARDAVERGL
KELESRQGYSRDMVQGALLCMDARTGAIRAMVGGRDFSKSEFNRATQSRRQPGSAFKPIIYAAAFDRGMTPATEIVDAPL
AFEDGMGHAWTPQNYDEEFLGPISLRTALVQSRNVVTIKLLQEIGVDYAIAYAANMGISSRLARDLSLALGSSAVTLQEL
VRSYAVLANGGKIVEPFMIKKIVDRTGYVFEEHTIKVEQAIDPRIAFLTTSILQDAVESGTGQRVKSIGRPVAGKTGTTN
DVHDAWFVGFTPSLVTGVWVGFDQQRPLGNQEVGGLAAAPIWLYCMERALANKPVESFPVPDGIVFVRRNEGALPEPFID
GTAPPDAIPADAEGSTSTEEEGEEDPGWKFRFPF
>Mature_674_residues
MVKTSQHPVTRNRCSAGSILIVLVFFLLLALLVGSSILYYVVLRDLPSIAALKEYRPSITTRVYADNNELIDEFYMENRK
LVRFEEIPSLVIKAFLAAEDARFFEHRGFDVQSISRAFFRNLRAGRIVQGGSTITQQVAKALYLSSEKSYSRKIKEAVLA
YKIDRSLSKKEILNLYLNHIYLGHGAYGIEAASQVYFGKRTRDLKLSEASLLAGLPKAPSSYDPYTSYQRARQRQTYVLS
RMVEDGHITEEEKNHVLQTPLPLRSIRPRDRVAAYFIENIRRYIQGKYGSDALYKEGLEVYTTLDIRTQQAARDAVERGL
KELESRQGYSRDMVQGALLCMDARTGAIRAMVGGRDFSKSEFNRATQSRRQPGSAFKPIIYAAAFDRGMTPATEIVDAPL
AFEDGMGHAWTPQNYDEEFLGPISLRTALVQSRNVVTIKLLQEIGVDYAIAYAANMGISSRLARDLSLALGSSAVTLQEL
VRSYAVLANGGKIVEPFMIKKIVDRTGYVFEEHTIKVEQAIDPRIAFLTTSILQDAVESGTGQRVKSIGRPVAGKTGTTN
DVHDAWFVGFTPSLVTGVWVGFDQQRPLGNQEVGGLAAAPIWLYCMERALANKPVESFPVPDGIVFVRRNEGALPEPFID
GTAPPDAIPADAEGSTSTEEEGEEDPGWKFRFPF

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=563, Percent_Identity=33.9253996447602, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI87082258, Length=312, Percent_Identity=42.3076923076923, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788867, Length=605, Percent_Identity=30.2479338842975, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789601, Length=172, Percent_Identity=40.1162790697674, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 75083; Mature: 75083

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKTSQHPVTRNRCSAGSILIVLVFFLLLALLVGSSILYYVVLRDLPSIAALKEYRPSIT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCE
TRVYADNNELIDEFYMENRKLVRFEEIPSLVIKAFLAAEDARFFEHRGFDVQSISRAFFR
EEEEECCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NLRAGRIVQGGSTITQQVAKALYLSSEKSYSRKIKEAVLAYKIDRSLSKKEILNLYLNHI
CCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHE
YLGHGAYGIEAASQVYFGKRTRDLKLSEASLLAGLPKAPSSYDPYTSYQRARQRQTYVLS
EECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
RMVEDGHITEEEKNHVLQTPLPLRSIRPRDRVAAYFIENIRRYIQGKYGSDALYKEGLEV
HHHHCCCCCCHHCCCEECCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEE
YTTLDIRTQQAARDAVERGLKELESRQGYSRDMVQGALLCMDARTGAIRAMVGGRDFSKS
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCEEEEECCCCCCHH
EFNRATQSRRQPGSAFKPIIYAAAFDRGMTPATEIVDAPLAFEDGMGHAWTPQNYDEEFL
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHC
GPISLRTALVQSRNVVTIKLLQEIGVDYAIAYAANMGISSRLARDLSLALGSSAVTLQEL
CHHHHHHHHHHCCCEEEEHHHHHHCCCEEEHHHHCCCHHHHHHHHHHHHHCCCHHHHHHH
VRSYAVLANGGKIVEPFMIKKIVDRTGYVFEEHTIKVEQAIDPRIAFLTTSILQDAVESG
HHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCEEEHHHHCCCHHHHHHHHHHHHHHHCC
TGQRVKSIGRPVAGKTGTTNDVHDAWFVGFTPSLVTGVWVGFDQQRPLGNQEVGGLAAAP
CCHHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
IWLYCMERALANKPVESFPVPDGIVFVRRNEGALPEPFIDGTAPPDAIPADAEGSTSTEE
HHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EGEEDPGWKFRFPF
CCCCCCCEEEECCC
>Mature Secondary Structure
MVKTSQHPVTRNRCSAGSILIVLVFFLLLALLVGSSILYYVVLRDLPSIAALKEYRPSIT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCE
TRVYADNNELIDEFYMENRKLVRFEEIPSLVIKAFLAAEDARFFEHRGFDVQSISRAFFR
EEEEECCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NLRAGRIVQGGSTITQQVAKALYLSSEKSYSRKIKEAVLAYKIDRSLSKKEILNLYLNHI
CCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHE
YLGHGAYGIEAASQVYFGKRTRDLKLSEASLLAGLPKAPSSYDPYTSYQRARQRQTYVLS
EECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
RMVEDGHITEEEKNHVLQTPLPLRSIRPRDRVAAYFIENIRRYIQGKYGSDALYKEGLEV
HHHHCCCCCCHHCCCEECCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEE
YTTLDIRTQQAARDAVERGLKELESRQGYSRDMVQGALLCMDARTGAIRAMVGGRDFSKS
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCEEEEECCCCCCHH
EFNRATQSRRQPGSAFKPIIYAAAFDRGMTPATEIVDAPLAFEDGMGHAWTPQNYDEEFL
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHC
GPISLRTALVQSRNVVTIKLLQEIGVDYAIAYAANMGISSRLARDLSLALGSSAVTLQEL
CHHHHHHHHHHCCCEEEEHHHHHHCCCEEEHHHHCCCHHHHHHHHHHHHHCCCHHHHHHH
VRSYAVLANGGKIVEPFMIKKIVDRTGYVFEEHTIKVEQAIDPRIAFLTTSILQDAVESG
HHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCEEEHHHHCCCHHHHHHHHHHHHHHHCC
TGQRVKSIGRPVAGKTGTTNDVHDAWFVGFTPSLVTGVWVGFDQQRPLGNQEVGGLAAAP
CCHHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
IWLYCMERALANKPVESFPVPDGIVFVRRNEGALPEPFIDGTAPPDAIPADAEGSTSTEE
HHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EGEEDPGWKFRFPF
CCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]