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The map label for this gene is ilvB [H]

Identifier: 85860815

GI number: 85860815

Start: 2980600

End: 2982273

Strand: Direct

Name: ilvB [H]

Synonym: SYN_01713

Alternate gene names: 85860815

Gene position: 2980600-2982273 (Clockwise)

Preceding gene: 85860813

Following gene: 85860817

Centisome position: 93.75

GC content: 56.93

Gene sequence:

>1674_bases
ATGGAAACCATCGGTGCGGACATTGTCCTGCAAATCCTTCAGGAGGAAGGTGTGGAGGCCATTTTCGGCTATCCCGGGGG
AAACTCTCTGCCTCTGCACGACCGGCTGCTGGGCAGCCCCATCCGTCATTACCTGACGCGGCATGAACAGGCCGCCGCGC
ATGCCGCTGACGGCTATGCCCGGGCTACCGGCAGAGTAGGTGTCTGCCTTTCCACGTCAGGACCGGGAGCGACCAACCTG
GTGACGGGCATCGCGACCGCCCATATGGATTCATCCCCCATTGTCGCTCTGACCTGCCAGGTCAACACCAGCCTCATGGG
ACGGGACGCTTTTCAGGAAACAGACATAATCGGGATTACACTGCCGATAACGAAGCACAGCTATCTGGTGCATGATGTGA
ACAATCTGGCCACCATCCTTCGCCAGTCCTTCAAACTGGCACGCTCGGGACGACCGGGACCGGTCCTGGTGGATATGCCC
CGGGATATCCTGGCTGCACGGTGTGAACGGGCGGCTCCCCGACAGGAAGCACCCGAAGCCACAGTGGAGCAGAGTCCCTC
GGATCGGCAACAACTGGAAAAAATTGCCAGGGCTCTGAATCAGAGTGAACGTCCCGTGATCATCATGGGCGGCGGCGTCA
AGTTGAGCAACTCCTGCGATCTCATGCTTGGACTCATTGAAAAAGGCGGTATTCCCTTTACCACCACGATGATGGGCGTG
GGTTCGATCACTTCCGGAAACGGCTATGATCTGGGTTTCATCGGCACTCACGGCAATCAGCTGGCGAACAGCATCGTTCA
TCAGTCTGATTTCATTCTGGCTGTCGGCATGCGCTTCTCGGATCGCAGCACTTCCCTCATTGATGAATTCGCCCCTCTGG
CCACCATTGCCCAGACTGACATCGATCCCACATCCATCGGCAAGAACGTTAAGGTTGCTTTCCCCTTTGTGGGTGAAATC
CGGGAAACGATATCCGCCCTGGTAAAACTGGTGGAACGGAAAGAAAGGACGCCATGGATGGAGCGCATACAGCAGGAGCG
GGAAACAACGGATAACTGCCTGCCCTACAAAGGGTGCGGAAAGGCAGGGCATTTTATCAGCCTGGTTCAGGACGCCATGC
CCAGGGAAACCATCGCGATTTCCGATGTGGGCTTGAACCAGATCTGGACGATGAGAACCTGGAAGGCGCGCACCCCACGC
AGCCTGATCACCAGCGGCGGCATGGGAACGATGGGATTCAGTGTACCGGCGGCAATGGGCGCCAAAATCGGCGCTCCTGA
TCGGTCGGTCGTAGCGATCTGTGGAGATGGCGGCTTCTACATGAATATTCAGGAACTGGCCACAATCAGCTATTACAATA
TCCCCGTGAAAATCATCGTCCTCAACAACGGCCACCTGGGGATGGTTCGTCAACTTCAGGATCTGTTTTATGAATCCCGT
TTCAGCACCGTGGATCTCGGCACGCATGTCGATCTGGTGACGGTTGCCAAAGGTTTCGGCATCCCAGCCCGCAGAATTTC
CGTGGACGACAGCCCCGAAGCGGCCCTTGCCGCCATGGCAGGCGCGGAAGGGCCTTATATGCTGGAGGTGATGGTTGACC
CGAACGATTATGTGTTCCCCATCATCCCTCCCGGCCATCCGAATATATCGATGATCCACGGCAAGATCTGCTGA

Upstream 100 bases:

>100_bases
ACTTAAAAAAATTTGCTTCCTCAAAAGGCTCCCCGCGGTGAAACGCCTTGCGTGGCCCTGAACAGGGAAGTGGTCCAGTC
TGAAAGGAGTATTAAAAATC

Downstream 100 bases:

>100_bases
GAAGAAAGCGTCTGGTTTTCTTATCCGACAGGAACAAACCAGGCAGGGCGGTCACAGTGAAATCAATAGAGGAGAAAGAT
CAGAAATGAGCATTGAAGAA

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
METIGADIVLQILQEEGVEAIFGYPGGNSLPLHDRLLGSPIRHYLTRHEQAAAHAADGYARATGRVGVCLSTSGPGATNL
VTGIATAHMDSSPIVALTCQVNTSLMGRDAFQETDIIGITLPITKHSYLVHDVNNLATILRQSFKLARSGRPGPVLVDMP
RDILAARCERAAPRQEAPEATVEQSPSDRQQLEKIARALNQSERPVIIMGGGVKLSNSCDLMLGLIEKGGIPFTTTMMGV
GSITSGNGYDLGFIGTHGNQLANSIVHQSDFILAVGMRFSDRSTSLIDEFAPLATIAQTDIDPTSIGKNVKVAFPFVGEI
RETISALVKLVERKERTPWMERIQQERETTDNCLPYKGCGKAGHFISLVQDAMPRETIAISDVGLNQIWTMRTWKARTPR
SLITSGGMGTMGFSVPAAMGAKIGAPDRSVVAICGDGGFYMNIQELATISYYNIPVKIIVLNNGHLGMVRQLQDLFYESR
FSTVDLGTHVDLVTVAKGFGIPARRISVDDSPEAALAAMAGAEGPYMLEVMVDPNDYVFPIIPPGHPNISMIHGKIC

Sequences:

>Translated_557_residues
METIGADIVLQILQEEGVEAIFGYPGGNSLPLHDRLLGSPIRHYLTRHEQAAAHAADGYARATGRVGVCLSTSGPGATNL
VTGIATAHMDSSPIVALTCQVNTSLMGRDAFQETDIIGITLPITKHSYLVHDVNNLATILRQSFKLARSGRPGPVLVDMP
RDILAARCERAAPRQEAPEATVEQSPSDRQQLEKIARALNQSERPVIIMGGGVKLSNSCDLMLGLIEKGGIPFTTTMMGV
GSITSGNGYDLGFIGTHGNQLANSIVHQSDFILAVGMRFSDRSTSLIDEFAPLATIAQTDIDPTSIGKNVKVAFPFVGEI
RETISALVKLVERKERTPWMERIQQERETTDNCLPYKGCGKAGHFISLVQDAMPRETIAISDVGLNQIWTMRTWKARTPR
SLITSGGMGTMGFSVPAAMGAKIGAPDRSVVAICGDGGFYMNIQELATISYYNIPVKIIVLNNGHLGMVRQLQDLFYESR
FSTVDLGTHVDLVTVAKGFGIPARRISVDDSPEAALAAMAGAEGPYMLEVMVDPNDYVFPIIPPGHPNISMIHGKIC
>Mature_557_residues
METIGADIVLQILQEEGVEAIFGYPGGNSLPLHDRLLGSPIRHYLTRHEQAAAHAADGYARATGRVGVCLSTSGPGATNL
VTGIATAHMDSSPIVALTCQVNTSLMGRDAFQETDIIGITLPITKHSYLVHDVNNLATILRQSFKLARSGRPGPVLVDMP
RDILAARCERAAPRQEAPEATVEQSPSDRQQLEKIARALNQSERPVIIMGGGVKLSNSCDLMLGLIEKGGIPFTTTMMGV
GSITSGNGYDLGFIGTHGNQLANSIVHQSDFILAVGMRFSDRSTSLIDEFAPLATIAQTDIDPTSIGKNVKVAFPFVGEI
RETISALVKLVERKERTPWMERIQQERETTDNCLPYKGCGKAGHFISLVQDAMPRETIAISDVGLNQIWTMRTWKARTPR
SLITSGGMGTMGFSVPAAMGAKIGAPDRSVVAICGDGGFYMNIQELATISYYNIPVKIIVLNNGHLGMVRQLQDLFYESR
FSTVDLGTHVDLVTVAKGFGIPARRISVDDSPEAALAAMAGAEGPYMLEVMVDPNDYVFPIIPPGHPNISMIHGKIC

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=485, Percent_Identity=26.5979381443299, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI21361361, Length=505, Percent_Identity=26.7326732673267, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=37.3865698729583, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=39.3501805054152, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1786717, Length=554, Percent_Identity=33.5740072202166, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=27.9279279279279, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788716, Length=555, Percent_Identity=25.7657657657658, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=488, Percent_Identity=28.0737704918033, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=488, Percent_Identity=28.0737704918033, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=594, Percent_Identity=24.2424242424242, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=569, Percent_Identity=36.2038664323374, Blast_Score=342, Evalue=7e-95,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=22.9674796747967, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320123, Length=291, Percent_Identity=24.0549828178694, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922626, Length=573, Percent_Identity=24.956369982548, Blast_Score=149, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59994; Mature: 59994

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00599 AA_TRANSFER_CLASS_2 ; PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIGADIVLQILQEEGVEAIFGYPGGNSLPLHDRLLGSPIRHYLTRHEQAAAHAADGYA
CCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCHH
RATGRVGVCLSTSGPGATNLVTGIATAHMDSSPIVALTCQVNTSLMGRDAFQETDIIGIT
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCHHCCCCCCCCCEEEEE
LPITKHSYLVHDVNNLATILRQSFKLARSGRPGPVLVDMPRDILAARCERAAPRQEAPEA
EECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCC
TVEQSPSDRQQLEKIARALNQSERPVIIMGGGVKLSNSCDLMLGLIEKGGIPFTTTMMGV
HHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCEEECCCCCHHHHHHHCCCCCEEEHHEEC
GSITSGNGYDLGFIGTHGNQLANSIVHQSDFILAVGMRFSDRSTSLIDEFAPLATIAQTD
CCCCCCCCEEEEEEECCHHHHHHHHHHCCCEEEEECCEECCCCHHHHHHHHHHHHHHHCC
IDPTSIGKNVKVAFPFVGEIRETISALVKLVERKERTPWMERIQQERETTDNCLPYKGCG
CCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
KAGHFISLVQDAMPRETIAISDVGLNQIWTMRTWKARTPRSLITSGGMGTMGFSVPAAMG
CCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCHHHHC
AKIGAPDRSVVAICGDGGFYMNIQELATISYYNIPVKIIVLNNGHLGMVRQLQDLFYESR
CCCCCCCCCEEEEECCCCEEEEHHHHEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHCC
FSTVDLGTHVDLVTVAKGFGIPARRISVDDSPEAALAAMAGAEGPYMLEVMVDPNDYVFP
CCEEECCCCEEEEEHHHCCCCCCEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCEEEE
IIPPGHPNISMIHGKIC
EECCCCCCEEEEEECCC
>Mature Secondary Structure
METIGADIVLQILQEEGVEAIFGYPGGNSLPLHDRLLGSPIRHYLTRHEQAAAHAADGYA
CCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCHH
RATGRVGVCLSTSGPGATNLVTGIATAHMDSSPIVALTCQVNTSLMGRDAFQETDIIGIT
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCHHCCCCCCCCCEEEEE
LPITKHSYLVHDVNNLATILRQSFKLARSGRPGPVLVDMPRDILAARCERAAPRQEAPEA
EECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCC
TVEQSPSDRQQLEKIARALNQSERPVIIMGGGVKLSNSCDLMLGLIEKGGIPFTTTMMGV
HHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCEEECCCCCHHHHHHHCCCCCEEEHHEEC
GSITSGNGYDLGFIGTHGNQLANSIVHQSDFILAVGMRFSDRSTSLIDEFAPLATIAQTD
CCCCCCCCEEEEEEECCHHHHHHHHHHCCCEEEEECCEECCCCHHHHHHHHHHHHHHHCC
IDPTSIGKNVKVAFPFVGEIRETISALVKLVERKERTPWMERIQQERETTDNCLPYKGCG
CCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
KAGHFISLVQDAMPRETIAISDVGLNQIWTMRTWKARTPRSLITSGGMGTMGFSVPAAMG
CCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCHHHHC
AKIGAPDRSVVAICGDGGFYMNIQELATISYYNIPVKIIVLNNGHLGMVRQLQDLFYESR
CCCCCCCCCEEEEECCCCEEEEHHHHEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHCC
FSTVDLGTHVDLVTVAKGFGIPARRISVDDSPEAALAAMAGAEGPYMLEVMVDPNDYVFP
CCEEECCCCEEEEEHHHCCCCCCEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCEEEE
IIPPGHPNISMIHGKIC
EECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]