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The map label for this gene is thiE

Identifier: 85860553

GI number: 85860553

Start: 2728601

End: 2729299

Strand: Direct

Name: thiE

Synonym: SYN_00302

Alternate gene names: 85860553

Gene position: 2728601-2729299 (Clockwise)

Preceding gene: 85860552

Following gene: 85860554

Centisome position: 85.82

GC content: 60.09

Gene sequence:

>699_bases
ATGACCATGATCCAGCCCAGGGCTCATTCCCGGAAAAAAATTGCCGGTCTGTATCTGGTCACGGACCGCGCTCTCTGCGG
TAACAGGGGTCTCGAAGAGGTCGTTTCCCTGGCCGTCCAGGGCGGAGCGGCCTGCGTGCAACTCCGGGAAAAGGATCTGC
CGACCAGGCCTTTTGTCGAAGAAGCAAAACGACTGAAAGCCCTCCTTTCCCCACTGCGCGTTCCTCTGATCATCAATGAC
CGGCTCGATGTGGCCCTGGCTGCCGAAGCCGACGGCGTCCACATCGGCCAGGAGGATATGCCCTGTGACATTGCCCGCCG
TCTGCTCGGGCCGAACGCCGTCATCGGCCTTTCCGTGGAAACCTGGGAAGACGTGGAAGCGGCGGAACTTATGGATGTCG
ATTATCTGGGAGTGAGCCCTGTTTTTGCCACGCCGACAAAAACGGACACCAAAGCCCCCTGGGGCCTGGAAGGGCTGACT
CGAATCCGAGCTTACTCCAGTCATCCCCTCGTCGCCATCGGTGGGATTTCCCGGGACAATGCGGCCGATGTCATCCTGGC
CGGCGCTGACTGCGCCGCTGTCGTCTCGGCCATCTGCGCCGCTTCCGACCCCCGGATGGCTGCTGCAGATATCCAGGCGG
AAATATCATGGGCACTGAAGAAGAAACAGAGAAACAAGCAGCATCTCATCCACCCTTAG

Upstream 100 bases:

>100_bases
TCTCTCCCGGGCCGGGCAGTCTTCAGCTTCACTTCCTCGACATCCTTTACCGGCTTACGGAAGAGGACATTGCACGCCGT
CTGCGGATCGTAGCGGGAGA

Downstream 100 bases:

>100_bases
ATAACACTATCCCGCAGGTTCCGTAATCCATGAAACCGGACAAGCTGATCATCCTTGACTACTCGGGCACACTCAGTCTT
CAGGCCGTACTGTTCGGCCG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTMIQPRAHSRKKIAGLYLVTDRALCGNRGLEEVVSLAVQGGAACVQLREKDLPTRPFVEEAKRLKALLSPLRVPLIIND
RLDVALAAEADGVHIGQEDMPCDIARRLLGPNAVIGLSVETWEDVEAAELMDVDYLGVSPVFATPTKTDTKAPWGLEGLT
RIRAYSSHPLVAIGGISRDNAADVILAGADCAAVVSAICAASDPRMAAADIQAEISWALKKKQRNKQHLIHP

Sequences:

>Translated_232_residues
MTMIQPRAHSRKKIAGLYLVTDRALCGNRGLEEVVSLAVQGGAACVQLREKDLPTRPFVEEAKRLKALLSPLRVPLIIND
RLDVALAAEADGVHIGQEDMPCDIARRLLGPNAVIGLSVETWEDVEAAELMDVDYLGVSPVFATPTKTDTKAPWGLEGLT
RIRAYSSHPLVAIGGISRDNAADVILAGADCAAVVSAICAASDPRMAAADIQAEISWALKKKQRNKQHLIHP
>Mature_231_residues
TMIQPRAHSRKKIAGLYLVTDRALCGNRGLEEVVSLAVQGGAACVQLREKDLPTRPFVEEAKRLKALLSPLRVPLIINDR
LDVALAAEADGVHIGQEDMPCDIARRLLGPNAVIGLSVETWEDVEAAELMDVDYLGVSPVFATPTKTDTKAPWGLEGLTR
IRAYSSHPLVAIGGISRDNAADVILAGADCAAVVSAICAASDPRMAAADIQAEISWALKKKQRNKQHLIHP

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=210, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=207, Percent_Identity=39.1304347826087, Blast_Score=118, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24927; Mature: 24796

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMIQPRAHSRKKIAGLYLVTDRALCGNRGLEEVVSLAVQGGAACVQLREKDLPTRPFVE
CCCCCCCCCCCHHHEEEEEEECCHHHCCCCHHHHHHHHHCCCHHEEEEHHCCCCCCHHHH
EAKRLKALLSPLRVPLIINDRLDVALAAEADGVHIGQEDMPCDIARRLLGPNAVIGLSVE
HHHHHHHHHHHHCCCEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHCCCCEEEEEEEC
TWEDVEAAELMDVDYLGVSPVFATPTKTDTKAPWGLEGLTRIRAYSSHPLVAIGGISRDN
CCCCCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCC
AADVILAGADCAAVVSAICAASDPRMAAADIQAEISWALKKKQRNKQHLIHP
CCCEEEECCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TMIQPRAHSRKKIAGLYLVTDRALCGNRGLEEVVSLAVQGGAACVQLREKDLPTRPFVE
CCCCCCCCCCHHHEEEEEEECCHHHCCCCHHHHHHHHHCCCHHEEEEHHCCCCCCHHHH
EAKRLKALLSPLRVPLIINDRLDVALAAEADGVHIGQEDMPCDIARRLLGPNAVIGLSVE
HHHHHHHHHHHHCCCEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHCCCCEEEEEEEC
TWEDVEAAELMDVDYLGVSPVFATPTKTDTKAPWGLEGLTRIRAYSSHPLVAIGGISRDN
CCCCCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCC
AADVILAGADCAAVVSAICAASDPRMAAADIQAEISWALKKKQRNKQHLIHP
CCCEEEECCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA