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The map label for this gene is thlA [H]

Identifier: 85860483

GI number: 85860483

Start: 2660566

End: 2661858

Strand: Direct

Name: thlA [H]

Synonym: SYN_02642

Alternate gene names: 85860483

Gene position: 2660566-2661858 (Clockwise)

Preceding gene: 85860482

Following gene: 85860484

Centisome position: 83.68

GC content: 59.16

Gene sequence:

>1293_bases
ATGAAAGATGTCGTCATCGTAAGCGGCGCCAGAACCGCCGTGGGTGCTTTTGGCGGATCGCTGAAAGGCGTGAGAGTTAC
GGATTTGGGAGCGCTGGTCATCAAAGAGGCCATCAAGAGAGCGGGGCTGCGGCCGGCCATCAGTGAAGAAGTGAAAGGCT
GCCGTTGCGATACCTTCGGAGAATTCGACAAGACCGAAATCAACAAGAAATATTATGATTACGATGAATCCCTGACCCCC
GTTTATTTCGACGAGTGCATCATGGGGAACTGCCTGATCGCCGGCCTGGGACAGAATCCCGGCCGTCAGTCCAGCATCTA
TGCCGGTCTGCCCGAAGAAACGAACACCATCACAGTGAACAAGGTCTGCGCATCCGGCATGAAAGCCATCACCCTGGCCG
CCCAGATCATCAAAGCCGGCGATGCCGACATCATGGTGGCCGGCGGCATGGAAAACATGAGCAATGTACCCTACGCCCTG
CCCGACGCCCGCTGGGGATACCGGATGAACATGCCTACGGGTTCCATCATCGACCTCATGGTTCATGATGGTCTCTGGGA
AATCTTCAACGGCTATCACATGGGATTCACGGCGGAAAATATCGCCTCCCGTTATGGAATCAGCCGTCAGGCCCAGGACG
AGCTGGCCCTCATGAGCCATCAGCGCGCCCGTGCGGCCATCGCCAGCGGCGCCGTCGCCGATGAAATCATCCCCGTTCCG
CTGCCCGTGAAGAAAGGCGCGGCTCCGCAGTTTTTCTCCGTCGACGAGCGTCCCATGGACACCAGCCTGGAAAAGATGGC
GAAGCTGGCCCCAGTCTTCAAGAAGGACGGAACCGTCACGGCGGCCAACGCCTCGGGTATCAATGACGGTGCGGCGGCTG
TCGTCGTGATGAGCGCCGACAAGGCAAAGGAACTGGGCCTCAAACCGCTGGCGAAGATCCTCGGCTATGCCTCCGGCGGC
GTCGATCCGGCATACATGGGTCTGGGTCCGATTCCGGCAACCCGCAAGGTCTTCAAGAAACTCGGCCTGACCATGAAGGA
CATGGACATCGTGGAACTGAACGAGGCCTTTGCATCCCAGGCCCTGGGCTGCGTCCAGGAAATGGGTGTGGATCTGGACA
AAACCAATCTCAACGGCAGCGGGATCTCCATCGGTCACCCCGTCGGCTGCACCGGCGCCCGGATCACCTACAGCTTGGCC
ATGCAGCTGCAGAAGAAGAACGCGCACCTCGGACTCGCCACGCTGTGTATCGGTGGCGGACAGGGGATGGCCATTGTCCT
GGAAAGAGTGTAA

Upstream 100 bases:

>100_bases
CCGCGCGAAGTGGAATTCGTGGAATCTCTTCAGGAAAAGGATAAGATTCACGCATCAAATTAAATCAAATATCAACATCC
AAGAAAGGAGATCGTTAACA

Downstream 100 bases:

>100_bases
GAACAGGTATTCAACCAGATAACTGATTAACGATTGACCCGAGGGCGGGGACGGTTCTGTATTTTCCGTCCCCGCTCCCT
GGATTCAGGAAGAGATCATG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKDVVIVSGARTAVGAFGGSLKGVRVTDLGALVIKEAIKRAGLRPAISEEVKGCRCDTFGEFDKTEINKKYYDYDESLTP
VYFDECIMGNCLIAGLGQNPGRQSSIYAGLPEETNTITVNKVCASGMKAITLAAQIIKAGDADIMVAGGMENMSNVPYAL
PDARWGYRMNMPTGSIIDLMVHDGLWEIFNGYHMGFTAENIASRYGISRQAQDELALMSHQRARAAIASGAVADEIIPVP
LPVKKGAAPQFFSVDERPMDTSLEKMAKLAPVFKKDGTVTAANASGINDGAAAVVVMSADKAKELGLKPLAKILGYASGG
VDPAYMGLGPIPATRKVFKKLGLTMKDMDIVELNEAFASQALGCVQEMGVDLDKTNLNGSGISIGHPVGCTGARITYSLA
MQLQKKNAHLGLATLCIGGGQGMAIVLERV

Sequences:

>Translated_430_residues
MKDVVIVSGARTAVGAFGGSLKGVRVTDLGALVIKEAIKRAGLRPAISEEVKGCRCDTFGEFDKTEINKKYYDYDESLTP
VYFDECIMGNCLIAGLGQNPGRQSSIYAGLPEETNTITVNKVCASGMKAITLAAQIIKAGDADIMVAGGMENMSNVPYAL
PDARWGYRMNMPTGSIIDLMVHDGLWEIFNGYHMGFTAENIASRYGISRQAQDELALMSHQRARAAIASGAVADEIIPVP
LPVKKGAAPQFFSVDERPMDTSLEKMAKLAPVFKKDGTVTAANASGINDGAAAVVVMSADKAKELGLKPLAKILGYASGG
VDPAYMGLGPIPATRKVFKKLGLTMKDMDIVELNEAFASQALGCVQEMGVDLDKTNLNGSGISIGHPVGCTGARITYSLA
MQLQKKNAHLGLATLCIGGGQGMAIVLERV
>Mature_430_residues
MKDVVIVSGARTAVGAFGGSLKGVRVTDLGALVIKEAIKRAGLRPAISEEVKGCRCDTFGEFDKTEINKKYYDYDESLTP
VYFDECIMGNCLIAGLGQNPGRQSSIYAGLPEETNTITVNKVCASGMKAITLAAQIIKAGDADIMVAGGMENMSNVPYAL
PDARWGYRMNMPTGSIIDLMVHDGLWEIFNGYHMGFTAENIASRYGISRQAQDELALMSHQRARAAIASGAVADEIIPVP
LPVKKGAAPQFFSVDERPMDTSLEKMAKLAPVFKKDGTVTAANASGINDGAAAVVVMSADKAKELGLKPLAKILGYASGG
VDPAYMGLGPIPATRKVFKKLGLTMKDMDIVELNEAFASQALGCVQEMGVDLDKTNLNGSGISIGHPVGCTGARITYSLA
MQLQKKNAHLGLATLCIGGGQGMAIVLERV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=430, Percent_Identity=42.3255813953488, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI4557237, Length=433, Percent_Identity=41.3394919168591, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI167614485, Length=435, Percent_Identity=38.8505747126437, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI4501853, Length=433, Percent_Identity=37.1824480369515, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4504327, Length=461, Percent_Identity=31.8872017353579, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI194353979, Length=428, Percent_Identity=27.803738317757, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI87082165, Length=431, Percent_Identity=46.6357308584687, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1788554, Length=432, Percent_Identity=49.537037037037, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI1787663, Length=435, Percent_Identity=38.3908045977011, Blast_Score=297, Evalue=8e-82,
Organism=Escherichia coli, GI48994986, Length=441, Percent_Identity=37.6417233560091, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1788683, Length=458, Percent_Identity=30.5676855895196, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI133906874, Length=430, Percent_Identity=39.5348837209302, Blast_Score=307, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI25147385, Length=431, Percent_Identity=35.4988399071926, Blast_Score=258, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI17535921, Length=433, Percent_Identity=37.6443418013857, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17551802, Length=461, Percent_Identity=30.8026030368764, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17535917, Length=437, Percent_Identity=27.9176201372998, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=431, Percent_Identity=38.2830626450116, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6322031, Length=429, Percent_Identity=34.4988344988345, Blast_Score=219, Evalue=7e-58,
Organism=Drosophila melanogaster, GI24655093, Length=431, Percent_Identity=40.8352668213457, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24640423, Length=434, Percent_Identity=40.7834101382488, Blast_Score=304, Evalue=7e-83,
Organism=Drosophila melanogaster, GI17648125, Length=436, Percent_Identity=38.302752293578, Blast_Score=267, Evalue=9e-72,
Organism=Drosophila melanogaster, GI17137578, Length=408, Percent_Identity=33.3333333333333, Blast_Score=185, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 45463; Mature: 45463

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVVIVSGARTAVGAFGGSLKGVRVTDLGALVIKEAIKRAGLRPAISEEVKGCRCDTFG
CCCEEEECCCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCC
EFDKTEINKKYYDYDESLTPVYFDECIMGNCLIAGLGQNPGRQSSIYAGLPEETNTITVN
CCCHHHHCCHHCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCCCEEEEH
KVCASGMKAITLAAQIIKAGDADIMVAGGMENMSNVPYALPDARWGYRMNMPTGSIIDLM
HHHHCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEECCCCCCCEEECCCCCCEEHHH
VHDGLWEIFNGYHMGFTAENIASRYGISRQAQDELALMSHQRARAAIASGAVADEIIPVP
HHCCHHHHHCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCEECCC
LPVKKGAAPQFFSVDERPMDTSLEKMAKLAPVFKKDGTVTAANASGINDGAAAVVVMSAD
CCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECC
KAKELGLKPLAKILGYASGGVDPAYMGLGPIPATRKVFKKLGLTMKDMDIVELNEAFASQ
CHHHCCHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ALGCVQEMGVDLDKTNLNGSGISIGHPVGCTGARITYSLAMQLQKKNAHLGLATLCIGGG
HHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECC
QGMAIVLERV
CCEEEEEECC
>Mature Secondary Structure
MKDVVIVSGARTAVGAFGGSLKGVRVTDLGALVIKEAIKRAGLRPAISEEVKGCRCDTFG
CCCEEEECCCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCC
EFDKTEINKKYYDYDESLTPVYFDECIMGNCLIAGLGQNPGRQSSIYAGLPEETNTITVN
CCCHHHHCCHHCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCCCEEEEH
KVCASGMKAITLAAQIIKAGDADIMVAGGMENMSNVPYALPDARWGYRMNMPTGSIIDLM
HHHHCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEECCCCCCCEEECCCCCCEEHHH
VHDGLWEIFNGYHMGFTAENIASRYGISRQAQDELALMSHQRARAAIASGAVADEIIPVP
HHCCHHHHHCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCEECCC
LPVKKGAAPQFFSVDERPMDTSLEKMAKLAPVFKKDGTVTAANASGINDGAAAVVVMSAD
CCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECC
KAKELGLKPLAKILGYASGGVDPAYMGLGPIPATRKVFKKLGLTMKDMDIVELNEAFASQ
CHHHCCHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ALGCVQEMGVDLDKTNLNGSGISIGHPVGCTGARITYSLAMQLQKKNAHLGLATLCIGGG
HHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECC
QGMAIVLERV
CCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]