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The map label for this gene is insH10 [H]

Identifier: 85860468

GI number: 85860468

Start: 2646383

End: 2647336

Strand: Direct

Name: insH10 [H]

Synonym: SYN_02657

Alternate gene names: 85860468

Gene position: 2646383-2647336 (Clockwise)

Preceding gene: 85860427

Following gene: 85860480

Centisome position: 83.24

GC content: 50.84

Gene sequence:

>954_bases
GTGACGAATGCCATCGACTGGAAACCCATCACCGCCATCCTGACCGAAAACTATCCGGTGGGCAAATCGGAATACGGAAA
CAAGGCTTATCCTCCTTTGATGCTGATGAAAGGGCTCCTCCTTCAGAAATGGTTCGGAATCAAGTCCGATCCCGAACTGG
AAAACCAGATCAACGACCGTTTATCCTTCAAAGCCTTTATCGGCTTACCCTTCAGTGACCCCTCACCGGATCATTCGATT
CTCTGCCGTTTCCGGGACAGAATCGGCAGGAAGGGGCTGGAAAAGGTTTTCAGCGAACTGCTGAAGCAATTCAAGAACCT
GGGCTTTTCCATCGAATCCGGTCTGGCTGTGGATGCCAGGATTATCCGCTCGGCCAGTCGTCCCGTAAGCAGAGACAAAC
TCGATGAGCTGCGGGAAAAGCGAAAACAGACAAAGAAGACGCCCCACTTCCAGCGGGATCTTGAATCCGACTGGACAGTG
AAGAACAAAAAACCCGTCTTCGGAATGAAGGAACATGCCTCCGTCGACGTAAAAAGCGGTCTCGTATTGTCCTCCTCGGT
TTCCAGAGCCTCCGAGCATGACACCAATTACTTCTCCTATGTGGTGGCCAACAGCCTTCAAGGGAAAGAGCTTCCTCCAA
GTGTTTACGCCGACAAGGGATACTGCAGCGAGGCCAATCGGGACTTCCTGAACATGAACGGCATTCGTGACGGAATCATG
CGGAAGGACCAGATCAATGCCAGGCTTACGGAAAATGAGATCAAGAGGAACAAGAAAATCTCCAAGGTTCGGTACAAGAT
CGAGCAGTATTTCGGAATTACCGAAAAGTATCATGGTGCAGGCAAGGCCCGCTTTACCTCTCTTGTCAAAGAGGGTTGGG
ACCATCTCTGCGGGGCGATGCCTTCAACATCAAAAAAATCGTTCTCGGCATCAGAAAGCAGGAAGCAACGGTAA

Upstream 100 bases:

>100_bases
CAGCATGTCATTCAAGAAATACGATTCTCATCCCTCTTTTTTTGATCTTGAAGTGAAGTAATCATTCGGAAACTCAAGAA
CTCAGCAATTCCTCTCCGAG

Downstream 100 bases:

>100_bases
CCCCATAGGGAAATTATGCAAAGAAACGCTTCCCCTGGTCATCCTGGGAGCTGAAAGATTTTGAAAGAGCAGTGTTAACG
CCGTAAAGGCGGCCGGAAGC

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTNAIDWKPITAILTENYPVGKSEYGNKAYPPLMLMKGLLLQKWFGIKSDPELENQINDRLSFKAFIGLPFSDPSPDHSI
LCRFRDRIGRKGLEKVFSELLKQFKNLGFSIESGLAVDARIIRSASRPVSRDKLDELREKRKQTKKTPHFQRDLESDWTV
KNKKPVFGMKEHASVDVKSGLVLSSSVSRASEHDTNYFSYVVANSLQGKELPPSVYADKGYCSEANRDFLNMNGIRDGIM
RKDQINARLTENEIKRNKKISKVRYKIEQYFGITEKYHGAGKARFTSLVKEGWDHLCGAMPSTSKKSFSASESRKQR

Sequences:

>Translated_317_residues
MTNAIDWKPITAILTENYPVGKSEYGNKAYPPLMLMKGLLLQKWFGIKSDPELENQINDRLSFKAFIGLPFSDPSPDHSI
LCRFRDRIGRKGLEKVFSELLKQFKNLGFSIESGLAVDARIIRSASRPVSRDKLDELREKRKQTKKTPHFQRDLESDWTV
KNKKPVFGMKEHASVDVKSGLVLSSSVSRASEHDTNYFSYVVANSLQGKELPPSVYADKGYCSEANRDFLNMNGIRDGIM
RKDQINARLTENEIKRNKKISKVRYKIEQYFGITEKYHGAGKARFTSLVKEGWDHLCGAMPSTSKKSFSASESRKQR
>Mature_316_residues
TNAIDWKPITAILTENYPVGKSEYGNKAYPPLMLMKGLLLQKWFGIKSDPELENQINDRLSFKAFIGLPFSDPSPDHSIL
CRFRDRIGRKGLEKVFSELLKQFKNLGFSIESGLAVDARIIRSASRPVSRDKLDELREKRKQTKKTPHFQRDLESDWTVK
NKKPVFGMKEHASVDVKSGLVLSSSVSRASEHDTNYFSYVVANSLQGKELPPSVYADKGYCSEANRDFLNMNGIRDGIMR
KDQINARLTENEIKRNKKISKVRYKIEQYFGITEKYHGAGKARFTSLVKEGWDHLCGAMPSTSKKSFSASESRKQR

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: COG3039

COG function: function code L; Transposase and inactivated derivatives, IS5 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=297, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788303, Length=297, Percent_Identity=26.9360269360269, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789921, Length=297, Percent_Identity=26.5993265993266, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789612, Length=297, Percent_Identity=26.5993265993266, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789355, Length=297, Percent_Identity=26.5993265993266, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788518, Length=297, Percent_Identity=26.5993265993266, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788342, Length=297, Percent_Identity=26.5993265993266, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787592, Length=297, Percent_Identity=26.5993265993266, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786877, Length=297, Percent_Identity=26.5993265993266, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786764, Length=297, Percent_Identity=26.5993265993266, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786453, Length=297, Percent_Identity=26.5993265993266, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 36168; Mature: 36037

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAIDWKPITAILTENYPVGKSEYGNKAYPPLMLMKGLLLQKWFGIKSDPELENQINDR
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LSFKAFIGLPFSDPSPDHSILCRFRDRIGRKGLEKVFSELLKQFKNLGFSIESGLAVDAR
HEEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
IIRSASRPVSRDKLDELREKRKQTKKTPHFQRDLESDWTVKNKKPVFGMKEHASVDVKSG
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCHHHCCEEHHCC
LVLSSSVSRASEHDTNYFSYVVANSLQGKELPPSVYADKGYCSEANRDFLNMNGIRDGIM
EEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCHHHCCCHHHHHC
RKDQINARLTENEIKRNKKISKVRYKIEQYFGITEKYHGAGKARFTSLVKEGWDHLCGAM
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCC
PSTSKKSFSASESRKQR
CCCCCCCCCCHHHHCCC
>Mature Secondary Structure 
TNAIDWKPITAILTENYPVGKSEYGNKAYPPLMLMKGLLLQKWFGIKSDPELENQINDR
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LSFKAFIGLPFSDPSPDHSILCRFRDRIGRKGLEKVFSELLKQFKNLGFSIESGLAVDAR
HEEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
IIRSASRPVSRDKLDELREKRKQTKKTPHFQRDLESDWTVKNKKPVFGMKEHASVDVKSG
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCHHHCCEEHHCC
LVLSSSVSRASEHDTNYFSYVVANSLQGKELPPSVYADKGYCSEANRDFLNMNGIRDGIM
EEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCHHHCCCHHHHHC
RKDQINARLTENEIKRNKKISKVRYKIEQYFGITEKYHGAGKARFTSLVKEGWDHLCGAM
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCC
PSTSKKSFSASESRKQR
CCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]