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The map label for this gene is leuB [H]

Identifier: 85860413

GI number: 85860413

Start: 2580078

End: 2581160

Strand: Direct

Name: leuB [H]

Synonym: SYN_00088

Alternate gene names: 85860413

Gene position: 2580078-2581160 (Clockwise)

Preceding gene: 85860411

Following gene: 85860414

Centisome position: 81.15

GC content: 57.99

Gene sequence:

>1083_bases
ATGCAGGAGGATTCCATGGGGACAGAATTTCATGTCGGTGTGATTCCCGGTGACGGAACAGGGCCGGAAGTCGTTGCGGA
AGGCATCAAAGTGCTCAATGCCGCGGCCAGTCGATACGGCTTCGGCCTGAAATATACCACCTACGACCTGGGCGGGGAAC
GCTACAAGCGGACAGGCGAAACCCTTCCGGACAGCGTGATCCGGGAACTGAAACAGCATAAGGCCATCTTTCTCGGCGCG
ATCGGTCATCCCGAAGTAGCACCGGGCATCCTCGAAAAGGGCATCCTGTTGAGGGCCCGCTTTGAACTGGATCAGTACAT
CAATCTGCGCCCCGTGAAACTTTATGAAGGCGTCGATACGCCTCTGAAAAACAAGGGACCGGAGGAAATCGATTTCGTGG
TCGTCCGGGAAAACACCGAAGGGCTCTATGCCGGCGCGGGCGGCGTCCTTAAAAGGGGAACCGCCGATGAAGTGGCCATC
CAGGAATCCATCAATACACGCAAGGGTGTGGAGCGCTGCATCCGCTTCGCCTTCGACATCACCCGAAAACGAAACAAGGG
AAAGAAACTGACCCTGTGCGGCAAAACCAACGTCCTGACCTTCGCCTTCGATCTCTGGGAACGAACCTTCTATGAAGTCG
CGAAGGACTATCCCGACATCGCGACGGATTACGCCCACGTGGACGCCACCTGCATGTGGATGGTCAAGAATCCCGAATGG
TTCGACGTCATTGTCACGGACAACATGTTCGGCGACATCATCACCGATCTGGGCGCCATGATCCAGGGCGGCATGGGCGT
TGCCGCCGGCGGCAACATCAATCCCGAGGGCGTCTCCATGTTCGAGCCCATCGGCGGCTCCGCGCCCAAATACACGGGCA
GGAACGTCATCAATCCCCTGGCCGCCATCATGGCCGGCGCCATGATGCTGGATTTTCTCGGTCAGGAGGAGGCGGCCCAG
TGCATCGAAACGTCGGTGCAGAAGGCCATCCGGAACGATCTCACCTCCATGGACGCCGGAAAGATGGGCATGGGGACCAA
GGAAGTGGGCGACCTGATCAGCCGGTACGTTCTGGAAAAATAA

Upstream 100 bases:

>100_bases
CACCGCTACCCCCTGCTTTATCCATGATCATTTTAATCACCCCTTAACTGAGAATTTCCTTGACATCGGACGGTAAGCCT
ATTAACGAATTCAGAAAAAA

Downstream 100 bases:

>100_bases
GGAAAATCAGGGCTTGACAAGCTCCGCTTTTTATTTTAAGGAGAGAAACGTTTTTAGAAAGGATCTGACTTCCGTGGCAA
ATAGAGAAAGGGCCGATATC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MQEDSMGTEFHVGVIPGDGTGPEVVAEGIKVLNAAASRYGFGLKYTTYDLGGERYKRTGETLPDSVIRELKQHKAIFLGA
IGHPEVAPGILEKGILLRARFELDQYINLRPVKLYEGVDTPLKNKGPEEIDFVVVRENTEGLYAGAGGVLKRGTADEVAI
QESINTRKGVERCIRFAFDITRKRNKGKKLTLCGKTNVLTFAFDLWERTFYEVAKDYPDIATDYAHVDATCMWMVKNPEW
FDVIVTDNMFGDIITDLGAMIQGGMGVAAGGNINPEGVSMFEPIGGSAPKYTGRNVINPLAAIMAGAMMLDFLGQEEAAQ
CIETSVQKAIRNDLTSMDAGKMGMGTKEVGDLISRYVLEK

Sequences:

>Translated_360_residues
MQEDSMGTEFHVGVIPGDGTGPEVVAEGIKVLNAAASRYGFGLKYTTYDLGGERYKRTGETLPDSVIRELKQHKAIFLGA
IGHPEVAPGILEKGILLRARFELDQYINLRPVKLYEGVDTPLKNKGPEEIDFVVVRENTEGLYAGAGGVLKRGTADEVAI
QESINTRKGVERCIRFAFDITRKRNKGKKLTLCGKTNVLTFAFDLWERTFYEVAKDYPDIATDYAHVDATCMWMVKNPEW
FDVIVTDNMFGDIITDLGAMIQGGMGVAAGGNINPEGVSMFEPIGGSAPKYTGRNVINPLAAIMAGAMMLDFLGQEEAAQ
CIETSVQKAIRNDLTSMDAGKMGMGTKEVGDLISRYVLEK
>Mature_360_residues
MQEDSMGTEFHVGVIPGDGTGPEVVAEGIKVLNAAASRYGFGLKYTTYDLGGERYKRTGETLPDSVIRELKQHKAIFLGA
IGHPEVAPGILEKGILLRARFELDQYINLRPVKLYEGVDTPLKNKGPEEIDFVVVRENTEGLYAGAGGVLKRGTADEVAI
QESINTRKGVERCIRFAFDITRKRNKGKKLTLCGKTNVLTFAFDLWERTFYEVAKDYPDIATDYAHVDATCMWMVKNPEW
FDVIVTDNMFGDIITDLGAMIQGGMGVAAGGNINPEGVSMFEPIGGSAPKYTGRNVINPLAAIMAGAMMLDFLGQEEAAQ
CIETSVQKAIRNDLTSMDAGKMGMGTKEVGDLISRYVLEK

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=322, Percent_Identity=32.2981366459627, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI28178816, Length=315, Percent_Identity=32.3809523809524, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI28178821, Length=315, Percent_Identity=32.3809523809524, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4758582, Length=354, Percent_Identity=28.8135593220339, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI28178838, Length=333, Percent_Identity=30.3303303303303, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI28178819, Length=161, Percent_Identity=36.0248447204969, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788101, Length=362, Percent_Identity=43.3701657458564, Blast_Score=293, Evalue=8e-81,
Organism=Escherichia coli, GI87081683, Length=364, Percent_Identity=35.4395604395604, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1787381, Length=388, Percent_Identity=28.3505154639175, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71986051, Length=352, Percent_Identity=31.5340909090909, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17550882, Length=333, Percent_Identity=32.4324324324324, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=326, Percent_Identity=29.4478527607362, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=326, Percent_Identity=28.2208588957055, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6322097, Length=354, Percent_Identity=34.180790960452, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=33.6065573770492, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=325, Percent_Identity=28.6153846153846, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=354, Percent_Identity=31.638418079096, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24643270, Length=327, Percent_Identity=30.5810397553517, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24643268, Length=327, Percent_Identity=30.5810397553517, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24661184, Length=336, Percent_Identity=30.0595238095238, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI161078633, Length=345, Percent_Identity=28.695652173913, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24650122, Length=345, Percent_Identity=28.695652173913, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161078635, Length=345, Percent_Identity=28.695652173913, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078637, Length=345, Percent_Identity=28.695652173913, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078639, Length=343, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281362242, Length=320, Percent_Identity=31.25, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24648872, Length=320, Percent_Identity=31.25, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI20130355, Length=339, Percent_Identity=21.5339233038348, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39368; Mature: 39368

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEDSMGTEFHVGVIPGDGTGPEVVAEGIKVLNAAASRYGFGLKYTTYDLGGERYKRTGE
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCC
TLPDSVIRELKQHKAIFLGAIGHPEVAPGILEKGILLRARFELDQYINLRPVKLYEGVDT
CCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCEEEEEEECHHHHCCCCEEEEECCCCC
PLKNKGPEEIDFVVVRENTEGLYAGAGGVLKRGTADEVAIQESINTRKGVERCIRFAFDI
CCCCCCCCCEEEEEEEECCCCEEECCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TRKRNKGKKLTLCGKTNVLTFAFDLWERTFYEVAKDYPDIATDYAHVDATCMWMVKNPEW
HHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEEECCCE
FDVIVTDNMFGDIITDLGAMIQGGMGVAAGGNINPEGVSMFEPIGGSAPKYTGRNVINPL
EEEEEECCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AAIMAGAMMLDFLGQEEAAQCIETSVQKAIRNDLTSMDAGKMGMGTKEVGDLISRYVLEK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQEDSMGTEFHVGVIPGDGTGPEVVAEGIKVLNAAASRYGFGLKYTTYDLGGERYKRTGE
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCC
TLPDSVIRELKQHKAIFLGAIGHPEVAPGILEKGILLRARFELDQYINLRPVKLYEGVDT
CCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCEEEEEEECHHHHCCCCEEEEECCCCC
PLKNKGPEEIDFVVVRENTEGLYAGAGGVLKRGTADEVAIQESINTRKGVERCIRFAFDI
CCCCCCCCCEEEEEEEECCCCEEECCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TRKRNKGKKLTLCGKTNVLTFAFDLWERTFYEVAKDYPDIATDYAHVDATCMWMVKNPEW
HHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEEECCCE
FDVIVTDNMFGDIITDLGAMIQGGMGVAAGGNINPEGVSMFEPIGGSAPKYTGRNVINPL
EEEEEECCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AAIMAGAMMLDFLGQEEAAQCIETSVQKAIRNDLTSMDAGKMGMGTKEVGDLISRYVLEK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA