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The map label for this gene is epsN [H]

Identifier: 85860110

GI number: 85860110

Start: 2317670

End: 2318827

Strand: Direct

Name: epsN [H]

Synonym: SYN_00827

Alternate gene names: 85860110

Gene position: 2317670-2318827 (Clockwise)

Preceding gene: 85860109

Following gene: 85860111

Centisome position: 72.9

GC content: 56.99

Gene sequence:

>1158_bases
GTGATTCCCGTCAACGAACCCCTGATCGGAGAACGGGAAGAGGACTATGTCCGGGAATGCCTCCGCAGCGGCTGGATTTC
CTCATCCGGCCGTTTTATTGAAGACTTTGAAGAGAAATGGGCGGGCTATTGCGGCAGGAAATTCGGGATCGCCGTCAGCA
GCGGTACGGCAGCCCTGCAGGTGGCTTTGGGCTGCATCGGCCTGGAACCGGGGGATCGGGTCATTCTCCCGACCTTCACC
ATTATATCCTGCGCGCTGGCTGTCATTTATAACGGCGGCGTTCCTGTCCTGGCGGATTGCGACCCGCGAACCTGGTGCCT
GTCTCCCGAAGGGGTAAAAGATGCCATCCAACGGGAAGTCCAAAAGGGGCATGGCAAGGAGTTGAAGGCTGTTATGCCCG
TTCACATTTACGGCCACCCTGTGGATATGGACTCTCTTCTGGAACTGGCGGACGCTTATTCCCTCACAGTCATTGAAGAC
GCGGCTGAAGCCCACGGCGCGGAGTATCTTACGGGTCGTTCCGGAAGCAATCCGTCATGGAAGCGATGCGGCGGACTGGG
AGACCTGGGGGTGTTCAGCTTTTACGCCAACAAGGCGATTACGACCGGGGAAGGCGGCATGGTGGTTACCGACGACGCGA
TGTTCGCCGAAAAGGCCCGCAGCCTGCGCAATCTCTGCTTTCGCCCGGAACAGCGATTTCTGCATACCGAAGCAGGTCAT
AACTTCCGCATGACCAACATCCAGGCAGCCATCGGGCTGGCCCAACTGGAGCGGATCGAGGCCATCCTGGAGAAAAAAAG
GGCTATTGGAAAAGCTTACAAGGAACGCCTGAGAGATCTGCCCGGCATGCAGCTGCCCGCGGAAGAAGCCTGGGCAAGAT
CGATTTACTGGATGTTCGGCATCGTGCTGGAGGAAATTACCGGCATGAACGCCGCCCAACTGGCAGAGCGGTTGCGGGAC
CAGGGCATTGATACCCGGCCGTTTTTTCTCGGAATGCACGAACAGCCCGTTTTTCAATCAATGGGACTTTTCCGGAATGA
AACCTATCCAGTGGCGGAAAGGCTGTCCCGTCAGGGACTTTATCTCCCCTCAGGACTGACGTTGACGGAATCACAGATTG
ACGAGGTTGCAGAGGCGGTAAAGAATGCCTTGTGTTGA

Upstream 100 bases:

>100_bases
CCTTTGAAATGCTGGAGGAAACCTCGATGATCGAAGTCAAGCAGGGGCCCTATACCGGCGAGGGCGATAAGACTCGTTTT
GATGACCAGGAGCCGGACGA

Downstream 100 bases:

>100_bases
AAGCTAATCTGATCCACGCGGGGAAGAAAACCGGCACCAGAAATAATCTTTCGAAATTCTTGTTTAATTTTTATGGTTGA
TACACCTTTTCAGAAACAAT

Product: perosamine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MIPVNEPLIGEREEDYVRECLRSGWISSSGRFIEDFEEKWAGYCGRKFGIAVSSGTAALQVALGCIGLEPGDRVILPTFT
IISCALAVIYNGGVPVLADCDPRTWCLSPEGVKDAIQREVQKGHGKELKAVMPVHIYGHPVDMDSLLELADAYSLTVIED
AAEAHGAEYLTGRSGSNPSWKRCGGLGDLGVFSFYANKAITTGEGGMVVTDDAMFAEKARSLRNLCFRPEQRFLHTEAGH
NFRMTNIQAAIGLAQLERIEAILEKKRAIGKAYKERLRDLPGMQLPAEEAWARSIYWMFGIVLEEITGMNAAQLAERLRD
QGIDTRPFFLGMHEQPVFQSMGLFRNETYPVAERLSRQGLYLPSGLTLTESQIDEVAEAVKNALC

Sequences:

>Translated_385_residues
MIPVNEPLIGEREEDYVRECLRSGWISSSGRFIEDFEEKWAGYCGRKFGIAVSSGTAALQVALGCIGLEPGDRVILPTFT
IISCALAVIYNGGVPVLADCDPRTWCLSPEGVKDAIQREVQKGHGKELKAVMPVHIYGHPVDMDSLLELADAYSLTVIED
AAEAHGAEYLTGRSGSNPSWKRCGGLGDLGVFSFYANKAITTGEGGMVVTDDAMFAEKARSLRNLCFRPEQRFLHTEAGH
NFRMTNIQAAIGLAQLERIEAILEKKRAIGKAYKERLRDLPGMQLPAEEAWARSIYWMFGIVLEEITGMNAAQLAERLRD
QGIDTRPFFLGMHEQPVFQSMGLFRNETYPVAERLSRQGLYLPSGLTLTESQIDEVAEAVKNALC
>Mature_385_residues
MIPVNEPLIGEREEDYVRECLRSGWISSSGRFIEDFEEKWAGYCGRKFGIAVSSGTAALQVALGCIGLEPGDRVILPTFT
IISCALAVIYNGGVPVLADCDPRTWCLSPEGVKDAIQREVQKGHGKELKAVMPVHIYGHPVDMDSLLELADAYSLTVIED
AAEAHGAEYLTGRSGSNPSWKRCGGLGDLGVFSFYANKAITTGEGGMVVTDDAMFAEKARSLRNLCFRPEQRFLHTEAGH
NFRMTNIQAAIGLAQLERIEAILEKKRAIGKAYKERLRDLPGMQLPAEEAWARSIYWMFGIVLEEITGMNAAQLAERLRD
QGIDTRPFFLGMHEQPVFQSMGLFRNETYPVAERLSRQGLYLPSGLTLTESQIDEVAEAVKNALC

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=401, Percent_Identity=31.6708229426434, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI2367285, Length=375, Percent_Identity=28.2666666666667, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42513; Mature: 42513

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVNEPLIGEREEDYVRECLRSGWISSSGRFIEDFEEKWAGYCGRKFGIAVSSGTAALQ
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHCEEECCCHHHHH
VALGCIGLEPGDRVILPTFTIISCALAVIYNGGVPVLADCDPRTWCLSPEGVKDAIQREV
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCCCEEECCCHHHHHHHHHHH
QKGHGKELKAVMPVHIYGHPVDMDSLLELADAYSLTVIEDAAEAHGAEYLTGRSGSNPSW
HHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHEEEEHHHHHHCCCCEECCCCCCCCCH
KRCGGLGDLGVFSFYANKAITTGEGGMVVTDDAMFAEKARSLRNLCFRPEQRFLHTEAGH
HHCCCCCCHHHHHHHHCCEEEECCCCEEEECHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
NFRMTNIQAAIGLAQLERIEAILEKKRAIGKAYKERLRDLPGMQLPAEEAWARSIYWMFG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
IVLEEITGMNAAQLAERLRDQGIDTRPFFLGMHEQPVFQSMGLFRNETYPVAERLSRQGL
HHHHHHCCCCHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHHCCC
YLPSGLTLTESQIDEVAEAVKNALC
CCCCCCEECHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPVNEPLIGEREEDYVRECLRSGWISSSGRFIEDFEEKWAGYCGRKFGIAVSSGTAALQ
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHCEEECCCHHHHH
VALGCIGLEPGDRVILPTFTIISCALAVIYNGGVPVLADCDPRTWCLSPEGVKDAIQREV
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCCCEEECCCHHHHHHHHHHH
QKGHGKELKAVMPVHIYGHPVDMDSLLELADAYSLTVIEDAAEAHGAEYLTGRSGSNPSW
HHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHEEEEHHHHHHCCCCEECCCCCCCCCH
KRCGGLGDLGVFSFYANKAITTGEGGMVVTDDAMFAEKARSLRNLCFRPEQRFLHTEAGH
HHCCCCCCHHHHHHHHCCEEEECCCCEEEECHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
NFRMTNIQAAIGLAQLERIEAILEKKRAIGKAYKERLRDLPGMQLPAEEAWARSIYWMFG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
IVLEEITGMNAAQLAERLRDQGIDTRPFFLGMHEQPVFQSMGLFRNETYPVAERLSRQGL
HHHHHHCCCCHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHHCCC
YLPSGLTLTESQIDEVAEAVKNALC
CCCCCCEECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]