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The map label for this gene is leuC2 [H]

Identifier: 85859747

GI number: 85859747

Start: 1982480

End: 1984411

Strand: Direct

Name: leuC2 [H]

Synonym: SYN_01409

Alternate gene names: 85859747

Gene position: 1982480-1984411 (Clockwise)

Preceding gene: 85859746

Following gene: 85859748

Centisome position: 62.36

GC content: 53.42

Gene sequence:

>1932_bases
ATGAAAAAAACTCTGACTCAGAAAATTCTTGAATCCCATATGGTATCCGGAAGTTACGTTCCGGGAAATGAAATAGCCAT
CCGAATCGACCAGACTCTGACTCAGGACGCCACGGGAACAATGGCCTATCTCCAGTTCGAATCCCTGCGTATATCCAGAG
TTAAAACTGAACTATCCGTCAGTTATGTCGATCACAATACAGTGCAGATCGGATATGAAAATGCCGATGATCATCGATAC
CTTCAAAGCGTCGCTGAAAAATTCGGCATTTACTTTTCCCGCCCGGGTAACGGCATCTGTCACCAGGTTCATCTGGAACG
GTTCGGCAGGCCGGGAAAAACCCTGATCGGTTCAGACAGTCATACGCCCACTGGTGGCGCTCTGGGAATGATCGCCATCG
GCGCCGGTGGCCTCGATGTCGCCCTGGCCATGGCAGGAGAACCTTTCTATCTGACCTGCCCGAAAGTGATTAAAATTTAT
CTTCTGGGACGTCTTCAGCCCTGGGTGTCCGCGAAGGACATCATTCTGAAAGTGCTCGATCTCTTCGGCACCAAAGGAAA
TGTGGGGTATGTATTTGAGTATGACGGCCCCGGACTTGAAACTCTGACAGTTCCTGAACGGGCAACGATCGCGAATATGG
GGGCGGAGTGCGGCGTGACGACCTCGGTTTTCCCCAGCGATGCCATGACCCGGCGTTTCCTGAAAGCGCAGCAGCGTGAA
GGAGACTGGATTGAAATTCGGGCCGACAGGGATTCCGAATACGACCGGGTTGTGGAGATCGATCTCTCCGAGTTGATCCC
GTTGACCGCGAAACCCAGCAGCCCGGGCAATATCTCCCCGATAAGGGAATGCGGCCCGGTCAAGATCCGGCAGGTCTGCC
TGGGGAGCTGCACCAATTCGTCATACAAGGATCTGGCTACAGTCGCGCAAATCCTGAAAGGAAAAGTAACTCATCCCGAC
GTCAGTTTCATTATCGCTCCCGGCTCCAAGCAGGTTCTTGAAAATCTCGCCCGTGACGGCCACCTTGCCGATCTGCTTTC
CGCCGGTGCGAGACTCATGGAAAATGCCTGTGGATTCTGCATTGGGAACAGTCAGAGTCCCCCGACTGGATCGGCCTCCC
TCAGAACATCCAATCGTAACTTTCCCGGACGGTCAGGCACGTTGGACGCAGAGGTCTATATCGCCAGTCCGGAAGCGGCT
GCCGCATCCATCATCACCGGTAAGATGACGGACCCGCGGGATCTGGGGATTCCCTACCCCGAGGTTTCCATGCCGGAAAC
CTTTTACGTCGATGACCGAATGATCCTCAAACCAAAGGATGCCGAGGCAGCCTCAGCGGTCATCATCTATCGCGGGCCGA
ATATCGGCCCGCCCCCGACCAACGATCCTCTCCCCATTCAACTGGATGGCGTAGTGACTCTGAAGGTCAGAGACAATATA
ACGACCGACCACATCATTCCCGCAGGTAAACGGATGAAGTACCGCTCCAACATCCCGAAATATGCGGAGTATCTCTTTGA
AATAGTGGACAATACATTTTATGAACGGGCGATGAAGATCAAGGAAAGCGGTCGCCACAATGTGATTGTGGCAGGACTCT
CCTACGGCCAGGGTTCTTCAAGGGAACATGCCGCACTATGCCCTATGTATGTGGGTGTGAAAGCCGTCCTTGCCAGATCC
TTTGAACGGATTCATGCCGCGAACCTGGTCAATTTCGGCATCCTCCCTCTTACTTTTCTCAACGAAGAAGACTATGACCG
ATTCGTACAGGGAGATGAAATTCACGTACCGGATATCCGGAACAGCCTCAGTGGCCCCGGTGTGCTGCAGATTCATAATG
TGAACAGGAACATCGTCATTGAAGTGTCCTTCCAACTCACCGAACGGCAGAAGCAGATCCTGCTGGCAGGCGGGGCCTTG
AACAGACGCTGA

Upstream 100 bases:

>100_bases
ATATACTGTCTGCCAATGGCTGCAGTTGAGAAATCCCATTTTGAAAGAATAAAAACCGATAAAAGGAAATTTGAAATCAC
CTCGAGGATAATGTCTTCTC

Downstream 100 bases:

>100_bases
TTCAAACATGATCTTTTGTACCCCATGATTTTATAAACACCGGTTCCGGCAGTCTGACATTGGAGGCCGCACTGAGAGGT
GAAAACAACAGGAGGGTTAT

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MKKTLTQKILESHMVSGSYVPGNEIAIRIDQTLTQDATGTMAYLQFESLRISRVKTELSVSYVDHNTVQIGYENADDHRY
LQSVAEKFGIYFSRPGNGICHQVHLERFGRPGKTLIGSDSHTPTGGALGMIAIGAGGLDVALAMAGEPFYLTCPKVIKIY
LLGRLQPWVSAKDIILKVLDLFGTKGNVGYVFEYDGPGLETLTVPERATIANMGAECGVTTSVFPSDAMTRRFLKAQQRE
GDWIEIRADRDSEYDRVVEIDLSELIPLTAKPSSPGNISPIRECGPVKIRQVCLGSCTNSSYKDLATVAQILKGKVTHPD
VSFIIAPGSKQVLENLARDGHLADLLSAGARLMENACGFCIGNSQSPPTGSASLRTSNRNFPGRSGTLDAEVYIASPEAA
AASIITGKMTDPRDLGIPYPEVSMPETFYVDDRMILKPKDAEAASAVIIYRGPNIGPPPTNDPLPIQLDGVVTLKVRDNI
TTDHIIPAGKRMKYRSNIPKYAEYLFEIVDNTFYERAMKIKESGRHNVIVAGLSYGQGSSREHAALCPMYVGVKAVLARS
FERIHAANLVNFGILPLTFLNEEDYDRFVQGDEIHVPDIRNSLSGPGVLQIHNVNRNIVIEVSFQLTERQKQILLAGGAL
NRR

Sequences:

>Translated_643_residues
MKKTLTQKILESHMVSGSYVPGNEIAIRIDQTLTQDATGTMAYLQFESLRISRVKTELSVSYVDHNTVQIGYENADDHRY
LQSVAEKFGIYFSRPGNGICHQVHLERFGRPGKTLIGSDSHTPTGGALGMIAIGAGGLDVALAMAGEPFYLTCPKVIKIY
LLGRLQPWVSAKDIILKVLDLFGTKGNVGYVFEYDGPGLETLTVPERATIANMGAECGVTTSVFPSDAMTRRFLKAQQRE
GDWIEIRADRDSEYDRVVEIDLSELIPLTAKPSSPGNISPIRECGPVKIRQVCLGSCTNSSYKDLATVAQILKGKVTHPD
VSFIIAPGSKQVLENLARDGHLADLLSAGARLMENACGFCIGNSQSPPTGSASLRTSNRNFPGRSGTLDAEVYIASPEAA
AASIITGKMTDPRDLGIPYPEVSMPETFYVDDRMILKPKDAEAASAVIIYRGPNIGPPPTNDPLPIQLDGVVTLKVRDNI
TTDHIIPAGKRMKYRSNIPKYAEYLFEIVDNTFYERAMKIKESGRHNVIVAGLSYGQGSSREHAALCPMYVGVKAVLARS
FERIHAANLVNFGILPLTFLNEEDYDRFVQGDEIHVPDIRNSLSGPGVLQIHNVNRNIVIEVSFQLTERQKQILLAGGAL
NRR
>Mature_643_residues
MKKTLTQKILESHMVSGSYVPGNEIAIRIDQTLTQDATGTMAYLQFESLRISRVKTELSVSYVDHNTVQIGYENADDHRY
LQSVAEKFGIYFSRPGNGICHQVHLERFGRPGKTLIGSDSHTPTGGALGMIAIGAGGLDVALAMAGEPFYLTCPKVIKIY
LLGRLQPWVSAKDIILKVLDLFGTKGNVGYVFEYDGPGLETLTVPERATIANMGAECGVTTSVFPSDAMTRRFLKAQQRE
GDWIEIRADRDSEYDRVVEIDLSELIPLTAKPSSPGNISPIRECGPVKIRQVCLGSCTNSSYKDLATVAQILKGKVTHPD
VSFIIAPGSKQVLENLARDGHLADLLSAGARLMENACGFCIGNSQSPPTGSASLRTSNRNFPGRSGTLDAEVYIASPEAA
AASIITGKMTDPRDLGIPYPEVSMPETFYVDDRMILKPKDAEAASAVIIYRGPNIGPPPTNDPLPIQLDGVVTLKVRDNI
TTDHIIPAGKRMKYRSNIPKYAEYLFEIVDNTFYERAMKIKESGRHNVIVAGLSYGQGSSREHAALCPMYVGVKAVLARS
FERIHAANLVNFGILPLTFLNEEDYDRFVQGDEIHVPDIRNSLSGPGVLQIHNVNRNIVIEVSFQLTERQKQILLAGGAL
NRR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=721, Percent_Identity=33.5644937586685, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI8659555, Length=835, Percent_Identity=24.311377245509, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI41352693, Length=658, Percent_Identity=25.531914893617, Blast_Score=161, Evalue=2e-39,
Organism=Escherichia coli, GI87081781, Length=723, Percent_Identity=25.8644536652835, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1786259, Length=469, Percent_Identity=27.2921108742004, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1787531, Length=488, Percent_Identity=27.6639344262295, Blast_Score=130, Evalue=4e-31,
Organism=Escherichia coli, GI2367097, Length=454, Percent_Identity=24.4493392070485, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=719, Percent_Identity=33.3796940194715, Blast_Score=314, Evalue=8e-86,
Organism=Caenorhabditis elegans, GI32564738, Length=680, Percent_Identity=33.9705882352941, Blast_Score=310, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25149342, Length=566, Percent_Identity=33.2155477031802, Blast_Score=258, Evalue=8e-69,
Organism=Caenorhabditis elegans, GI17568399, Length=815, Percent_Identity=25.8895705521472, Blast_Score=167, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322261, Length=727, Percent_Identity=33.2874828060523, Blast_Score=354, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6323335, Length=702, Percent_Identity=32.9059829059829, Blast_Score=322, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6320440, Length=618, Percent_Identity=28.1553398058252, Blast_Score=193, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321429, Length=688, Percent_Identity=25, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI281365315, Length=722, Percent_Identity=34.0720221606648, Blast_Score=321, Evalue=1e-87,
Organism=Drosophila melanogaster, GI17864292, Length=722, Percent_Identity=34.0720221606648, Blast_Score=321, Evalue=1e-87,
Organism=Drosophila melanogaster, GI28571643, Length=715, Percent_Identity=33.2867132867133, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI161076999, Length=687, Percent_Identity=34.2066957787482, Blast_Score=311, Evalue=7e-85,
Organism=Drosophila melanogaster, GI24645686, Length=500, Percent_Identity=24.6, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17137564, Length=501, Percent_Identity=24.3512974051896, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 70338; Mature: 70338

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLTQKILESHMVSGSYVPGNEIAIRIDQTLTQDATGTMAYLQFESLRISRVKTELSV
CCHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHCCCCCCCEEEEEEEHHEEEEEHEEEEE
SYVDHNTVQIGYENADDHRYLQSVAEKFGIYFSRPGNGICHQVHLERFGRPGKTLIGSDS
EEECCCEEEEECCCCCHHHHHHHHHHHHCEEEECCCCCCEEEHHHHHCCCCCCEEECCCC
HTPTGGALGMIAIGAGGLDVALAMAGEPFYLTCPKVIKIYLLGRLQPWVSAKDIILKVLD
CCCCCCCEEEEEECCCCCEEEEEECCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHH
LFGTKGNVGYVFEYDGPGLETLTVPERATIANMGAECGVTTSVFPSDAMTRRFLKAQQRE
HHCCCCCEEEEEEECCCCCEEEECCCCHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCC
GDWIEIRADRDSEYDRVVEIDLSELIPLTAKPSSPGNISPIRECGPVKIRQVCLGSCTNS
CCEEEEEECCCCCCCEEEEEEHHHCCCCEECCCCCCCCCCHHHCCCHHHHHHHHCCCCCC
SYKDLATVAQILKGKVTHPDVSFIIAPGSKQVLENLARDGHLADLLSAGARLMENACGFC
CHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEE
IGNSQSPPTGSASLRTSNRNFPGRSGTLDAEVYIASPEAAAASIITGKMTDPRDLGIPYP
ECCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCHHHHHEEECCCCCCHHCCCCCC
EVSMPETFYVDDRMILKPKDAEAASAVIIYRGPNIGPPPTNDPLPIQLDGVVTLKVRDNI
CCCCCCEEEECCEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCEEEEEEECCC
TTDHIIPAGKRMKYRSNIPKYAEYLFEIVDNTFYERAMKIKESGRHNVIVAGLSYGQGSS
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
REHAALCPMYVGVKAVLARSFERIHAANLVNFGILPLTFLNEEDYDRFVQGDEIHVPDIR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCEECCHHH
NSLSGPGVLQIHNVNRNIVIEVSFQLTERQKQILLAGGALNRR
HCCCCCCEEEEEECCCEEEEEEEEEECCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKKTLTQKILESHMVSGSYVPGNEIAIRIDQTLTQDATGTMAYLQFESLRISRVKTELSV
CCHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHCCCCCCCEEEEEEEHHEEEEEHEEEEE
SYVDHNTVQIGYENADDHRYLQSVAEKFGIYFSRPGNGICHQVHLERFGRPGKTLIGSDS
EEECCCEEEEECCCCCHHHHHHHHHHHHCEEEECCCCCCEEEHHHHHCCCCCCEEECCCC
HTPTGGALGMIAIGAGGLDVALAMAGEPFYLTCPKVIKIYLLGRLQPWVSAKDIILKVLD
CCCCCCCEEEEEECCCCCEEEEEECCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHH
LFGTKGNVGYVFEYDGPGLETLTVPERATIANMGAECGVTTSVFPSDAMTRRFLKAQQRE
HHCCCCCEEEEEEECCCCCEEEECCCCHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCC
GDWIEIRADRDSEYDRVVEIDLSELIPLTAKPSSPGNISPIRECGPVKIRQVCLGSCTNS
CCEEEEEECCCCCCCEEEEEEHHHCCCCEECCCCCCCCCCHHHCCCHHHHHHHHCCCCCC
SYKDLATVAQILKGKVTHPDVSFIIAPGSKQVLENLARDGHLADLLSAGARLMENACGFC
CHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEE
IGNSQSPPTGSASLRTSNRNFPGRSGTLDAEVYIASPEAAAASIITGKMTDPRDLGIPYP
ECCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCHHHHHEEECCCCCCHHCCCCCC
EVSMPETFYVDDRMILKPKDAEAASAVIIYRGPNIGPPPTNDPLPIQLDGVVTLKVRDNI
CCCCCCEEEECCEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCEEEEEEECCC
TTDHIIPAGKRMKYRSNIPKYAEYLFEIVDNTFYERAMKIKESGRHNVIVAGLSYGQGSS
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
REHAALCPMYVGVKAVLARSFERIHAANLVNFGILPLTFLNEEDYDRFVQGDEIHVPDIR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCEECCHHH
NSLSGPGVLQIHNVNRNIVIEVSFQLTERQKQILLAGGALNRR
HCCCCCCEEEEEECCCEEEEEEEEEECCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]