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The map label for this gene is cwlS [H]

Identifier: 85859746

GI number: 85859746

Start: 1980901

End: 1982019

Strand: Direct

Name: cwlS [H]

Synonym: SYN_01408

Alternate gene names: 85859746

Gene position: 1980901-1982019 (Clockwise)

Preceding gene: 85859742

Following gene: 85859747

Centisome position: 62.31

GC content: 48.7

Gene sequence:

>1119_bases
ATGTGGCCACCCCCGATCAGACTAAGGAGGTACTTCATGACAAAACGGCGGATTTTCTTTTGGATGGGATTGGCGCTCCT
GTTCGTGTTTGGTCTTTTTTCTCAGGAAGGACTTGCAAAGGAAACCTACAAAGTAAAACGCGGCGATACACTCAGCGGAA
TCGCCGACCATCTCGGCGTCTCCGTCAAGGAATTGAAGCGGGCCAATGGTTTGAAAAAAACAGCTCTCAAGCCCAGTCAA
ATTCTCACCATTCCCCAGAAATCCAAAAAACAGACAGCAGAAAAACCCATAAAGACGTCCCGAAAGACTTCAGAGAAGGC
TTCCGAGTCGAAATCTGAGTATTATACAGTCCGGAAAGGAGACACTCTGGGCGGCATTTCCAAAAAGACGGGAGTTTCCA
TCGGTGAGCTTCAAGCACTGAATCGAGTCCAGGTCCGCGCTTTGAAGCCTGGCCAGAAGCTTACATTATCCAAACGGGAA
TCCGTCCCGACGGCAAGAAAAGAGAGCATACGCCATCAGGCGCCTCCGGCAGTGGAAACGTTTGAAGAGGAATTCTATCT
GGAAGATGGCCAGGAAGAAGATGAAATTGTCGATGAGGATTGGGCATCCGTCGAGAACGACAGGGCAGTCAGTTCCAACT
TGCTTGGCAAATGGAACAGCCCTCATGAAAGGCAGATTTTTATCCGTGTCGCAAAAGGATTCCTGGGGACTCCATACCGT
TTTGGCGGCTCATCCGTCCGCGGCATTGATTGTTCGGCCTTTGTTGCAAAAGTTTATCAATTTTTTGATGTGAATTTGCC
CCGGACGGCGCGGGAACAGTCCCGCATGGGCGTGCGGGTGGCAAAAAATGAACTTCAGGAAGGCGATCTGGTCTTTTTCA
ACACGAGGCGCGCCTTCGGCCATGTCGGGATTTACATCGGGAACAATGAATTCATTCATGCCTCTTCAGGACGCAGCTCT
GGAAAGAACGTCAGAATAGACAGTCTTGACAAGCCATATTATAATAAACGATTTATCAAGGCTGTTCGTGTTAAGGGCAT
GAAGGAAAAGGTTGCGGAAGAACGTGATGTTTCCTTATTAGAATCATCACTTCAGCTGTCAGCAAATGCGGTAAACTGA

Upstream 100 bases:

>100_bases
TAAAAAAATAAGTTTGACTTATTGGAATAATTTTTATAAATATTCGCCGATTTTGTGTACAGTTTGTCATCCAATGTCAG
AATCTGTGCACCGTTAGACA

Downstream 100 bases:

>100_bases
ATTTTCCAAAAATGATCTGACATTTACGGCATCGAATCCCGGCCGGGATTCGATGCCGTTTTCTTTGGGAATTTTTCAAC
TCCCTTTTTCTTTCCTCATC

Product: cell wall-associated hydrolase

Products: NA

Alternate protein names: Cell wall lytic enzyme associated with cell separation [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MWPPPIRLRRYFMTKRRIFFWMGLALLFVFGLFSQEGLAKETYKVKRGDTLSGIADHLGVSVKELKRANGLKKTALKPSQ
ILTIPQKSKKQTAEKPIKTSRKTSEKASESKSEYYTVRKGDTLGGISKKTGVSIGELQALNRVQVRALKPGQKLTLSKRE
SVPTARKESIRHQAPPAVETFEEEFYLEDGQEEDEIVDEDWASVENDRAVSSNLLGKWNSPHERQIFIRVAKGFLGTPYR
FGGSSVRGIDCSAFVAKVYQFFDVNLPRTAREQSRMGVRVAKNELQEGDLVFFNTRRAFGHVGIYIGNNEFIHASSGRSS
GKNVRIDSLDKPYYNKRFIKAVRVKGMKEKVAEERDVSLLESSLQLSANAVN

Sequences:

>Translated_372_residues
MWPPPIRLRRYFMTKRRIFFWMGLALLFVFGLFSQEGLAKETYKVKRGDTLSGIADHLGVSVKELKRANGLKKTALKPSQ
ILTIPQKSKKQTAEKPIKTSRKTSEKASESKSEYYTVRKGDTLGGISKKTGVSIGELQALNRVQVRALKPGQKLTLSKRE
SVPTARKESIRHQAPPAVETFEEEFYLEDGQEEDEIVDEDWASVENDRAVSSNLLGKWNSPHERQIFIRVAKGFLGTPYR
FGGSSVRGIDCSAFVAKVYQFFDVNLPRTAREQSRMGVRVAKNELQEGDLVFFNTRRAFGHVGIYIGNNEFIHASSGRSS
GKNVRIDSLDKPYYNKRFIKAVRVKGMKEKVAEERDVSLLESSLQLSANAVN
>Mature_372_residues
MWPPPIRLRRYFMTKRRIFFWMGLALLFVFGLFSQEGLAKETYKVKRGDTLSGIADHLGVSVKELKRANGLKKTALKPSQ
ILTIPQKSKKQTAEKPIKTSRKTSEKASESKSEYYTVRKGDTLGGISKKTGVSIGELQALNRVQVRALKPGQKLTLSKRE
SVPTARKESIRHQAPPAVETFEEEFYLEDGQEEDEIVDEDWASVENDRAVSSNLLGKWNSPHERQIFIRVAKGFLGTPYR
FGGSSVRGIDCSAFVAKVYQFFDVNLPRTAREQSRMGVRVAKNELQEGDLVFFNTRRAFGHVGIYIGNNEFIHASSGRSS
GKNVRIDSLDKPYYNKRFIKAVRVKGMKEKVAEERDVSLLESSLQLSANAVN

Specific function: Probably functions as a cell separation enzyme in addition to lytE and lytF [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cell surface. Note=Localized at cell separation sites and cell poles during late vegetative phase [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1788501, Length=110, Percent_Identity=49.0909090909091, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788001, Length=112, Percent_Identity=47.3214285714286, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787944, Length=116, Percent_Identity=40.5172413793103, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786421, Length=154, Percent_Identity=33.7662337662338, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786405, Length=119, Percent_Identity=42.0168067226891, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: =3.4.19.11 [H]

Molecular weight: Translated: 42002; Mature: 42002

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWPPPIRLRRYFMTKRRIFFWMGLALLFVFGLFSQEGLAKETYKVKRGDTLSGIADHLGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHCC
SVKELKRANGLKKTALKPSQILTIPQKSKKQTAEKPIKTSRKTSEKASESKSEYYTVRKG
CHHHHHHHCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECC
DTLGGISKKTGVSIGELQALNRVQVRALKPGQKLTLSKRESVPTARKESIRHQAPPAVET
CCCCCCCHHCCCCHHHHHHHHHHEEEEECCCCCEECCHHHCCCCHHHHHHHHCCCCHHHH
FEEEFYLEDGQEEDEIVDEDWASVENDRAVSSNLLGKWNSPHERQIFIRVAKGFLGTPYR
HHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHEEHHHHCCCCEE
FGGSSVRGIDCSAFVAKVYQFFDVNLPRTAREQSRMGVRVAKNELQEGDLVFFNTRRAFG
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCC
HVGIYIGNNEFIHASSGRSSGKNVRIDSLDKPYYNKRFIKAVRVKGMKEKVAEERDVSLL
EEEEEECCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
ESSLQLSANAVN
HHHHHHHCCCCC
>Mature Secondary Structure
MWPPPIRLRRYFMTKRRIFFWMGLALLFVFGLFSQEGLAKETYKVKRGDTLSGIADHLGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHCC
SVKELKRANGLKKTALKPSQILTIPQKSKKQTAEKPIKTSRKTSEKASESKSEYYTVRKG
CHHHHHHHCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECC
DTLGGISKKTGVSIGELQALNRVQVRALKPGQKLTLSKRESVPTARKESIRHQAPPAVET
CCCCCCCHHCCCCHHHHHHHHHHEEEEECCCCCEECCHHHCCCCHHHHHHHHCCCCHHHH
FEEEFYLEDGQEEDEIVDEDWASVENDRAVSSNLLGKWNSPHERQIFIRVAKGFLGTPYR
HHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHEEHHHHCCCCEE
FGGSSVRGIDCSAFVAKVYQFFDVNLPRTAREQSRMGVRVAKNELQEGDLVFFNTRRAFG
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCC
HVGIYIGNNEFIHASSGRSSGKNVRIDSLDKPYYNKRFIKAVRVKGMKEKVAEERDVSLL
EEEEEECCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
ESSLQLSANAVN
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377 [H]