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The map label for this gene is epsE [H]

Identifier: 85859742

GI number: 85859742

Start: 1973829

End: 1976183

Strand: Direct

Name: epsE [H]

Synonym: SYN_01404

Alternate gene names: 85859742

Gene position: 1973829-1976183 (Clockwise)

Preceding gene: 85859738

Following gene: 85859746

Centisome position: 62.08

GC content: 49.17

Gene sequence:

>2355_bases
TTGGCTCATCTTATTATTTATTTGCACACCCGAGAACACGGACCGCCGTGCAGGGAGAAAGTCATGACAGACATTCTGAC
AAAAAATCATCAAATTTCCGAACTCGAGGAAAAACTCCTGTTACGGAAGAAATTGCAGGACATTACCAACCAGATTCATG
CCGCCCGGAATATCAAACAGATCCTTGTAGACCTCAAAGAAGGCATACTGAACCTTTTCGACGCGTATTCCATTACAATC
TATGTCGTCGATCGTTTAAGGAACGAGATTTTCTCCCTGCTGCTCTCCGCCTCCCAGATAAAAGAAATCCGTGTGCCCAT
CAACAATAAAAGCATTGCCGGCTACGTGGCCAATAACAAAAAAACCGTCAATATCGCCGATGCTTATGATGTCGCGGAAC
TGAGACAAATTGATCCCGAGTTGAGCTTCGACTTGAGCTGGGACAAAAAGACCGGGTTCCGCTCTCGACAGATTCTCTGC
TCTCCAGTTTTCTACAACGGCAAGCTCTCCGGTGTCATTCAGATTCTCAACAAGAAAGGCGGGGGTAAATTTTCGGATGA
AGAAGTGGGTTTTGTCCAGGAAATCGCGGAAGTTCTCGGTGTCGCTTTTTTCAACCAGGAACGATATGCCCGCCGTCGGA
AAACCCGTTATGACTACCTCATCAGCAGAGATCTGCTGACGGAAGAAGAACTTGACAACGCGTGGGAGGAAGCACGGGAC
AAGAAGGAACCGATGGAATCTTTCCTCATGAAAAAGTACGACGTTTCCAAGGAAGACATCGGAAAGTCTCTGTCGGAATT
TTATCACTGTAAATTCATACAGTTCGATGACAAATTCCCCATCCCCGGCGATTTGCTGAAAAACCTCAAGCGGGAATATC
TGCGTCGTGAATTATGGGTTCCTCTCTGCAAAACCGATGACAAAATCCAGGTTATCGTTGATGATCCGAACAATATCCTG
AAACGGGATATGATAGAGAGTCTTTTAAAAACAAAGGCCGTCAAATACGACGTTGCCTTTCCGGAAGACATCATAAGGTA
TATCAACTATTTCTTTCAGATGCCTGCAGATGAATCCTCCTTTACCGAACTTCTCGGCAAACTGGACGAAGACGAAGAAA
CGCCGGAAGAAGACGGTGAAATCGTTACGGAATCGGACAGCGTCATTATGCAGCTCGTTAACAAGATCATCAACGATGCC
TATTTTCGACGCAGTTCCGATATCCACATTGAACCGAACGTCGCGAAAAAAAACGTGGATATCCGCTTCCGCGTCGACGG
CGACTGTTCGCTTTATCAGACGGTGCCCTTTAATTACCGGGCTGCCCTCGTCTCACGCATAAAGATCATGTCCAACCTGG
ATATTACGGTAAAAAGAATCCCCCAGGATGGAAAAATCAAGTTCAAAAGTGTCAATGGAGATGAAATTGAACTCCGGGTT
GCCACCATACCTACACAGGGCAACGTGGAAGACGTTGTCATGCGTATCCTGGCAAAAGGTGAAACTCTGCCTCTTGAAGC
TATGGGCATGATGGATCGCAATTACGGTGAAATGCTCAAGATCCTGGAAAAGCCCTATGGCATGGTCCTTGTTGTCGGCC
CGACCGGATCGGGAAAGACCACCACGCTGCATGCAGCCCTTCGCCACATCAACACCCCGGAAAGGAAGATATGGACGGCT
GAAGATCCGGTCGAAATAACGCAATACGGATTGCGGCAGGTCCAGGTTCAGCCAAAGATCGGTTTTGATTTTGCCGCCGC
GATGCGAGCCTTTCTCCGTGCCGACCCGGATGTCATCATGGTCGGTGAAATGCGAGATTTTGAAACGGCGAAGACCGGTG
TCGAGGCGTCCCTGACGGGGCATCTTGTCTTCAGCACCCTGCATACAAACAGCGCCCCGGAAACCATTACCCGGTTGCTG
GATATGGGCATTGACCCTCTCAATTTTTCCGATGCCCTGCTCTGCATTCTCGCCCAGCGACTGGTCAGGACTCTGTGCAA
AAAGTGCAAGGAAGCCTATCATCCAACCCGGGCCGAATATGACGAAATAGCAGAAAGTTACGGTCTGGAGCACTTTGCCC
GACTGAATCTTCCTTACACGGATGACTTTGTCCTCTACCGCCCCAAGGGCTGCGACGCCTGCGACCGGACCGGCTACAAA
GGAAGGATGGGCATTCACGAACTGCTGATCGGTTCGGATTCGATCAAGCGCCTGATCCAGAAACATGCCGATATCGATAC
GATGCGGGAGACTGCTATAGTCGAAGGCATGACAACCCTTCTGCAGGATGGCATCAGAAAGGCGATTCAGGGGATGACCG
ATTTCAGACAGGTTCGCAGGGTCTGCATCAAATAG

Upstream 100 bases:

>100_bases
TATATAAAATAATTCACATTTACTTACTTTATTCTTGATCTTTAAAAAGGATTATATTAGTGTCCAGCGTTAAATTGATC
AACCTGCCAATATCCGTTTA

Downstream 100 bases:

>100_bases
GACGGTTCAGCCTTCTTTAAGCCTTTCCTGCCAGTTCAGCATCATGTTGAGGGCATCATGCGGCTCCACGCGGGAAATGT
TCAACGCCGCAAGCTCTTCG

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 784; Mature: 783

Protein sequence:

>784_residues
MAHLIIYLHTREHGPPCREKVMTDILTKNHQISELEEKLLLRKKLQDITNQIHAARNIKQILVDLKEGILNLFDAYSITI
YVVDRLRNEIFSLLLSASQIKEIRVPINNKSIAGYVANNKKTVNIADAYDVAELRQIDPELSFDLSWDKKTGFRSRQILC
SPVFYNGKLSGVIQILNKKGGGKFSDEEVGFVQEIAEVLGVAFFNQERYARRRKTRYDYLISRDLLTEEELDNAWEEARD
KKEPMESFLMKKYDVSKEDIGKSLSEFYHCKFIQFDDKFPIPGDLLKNLKREYLRRELWVPLCKTDDKIQVIVDDPNNIL
KRDMIESLLKTKAVKYDVAFPEDIIRYINYFFQMPADESSFTELLGKLDEDEETPEEDGEIVTESDSVIMQLVNKIINDA
YFRRSSDIHIEPNVAKKNVDIRFRVDGDCSLYQTVPFNYRAALVSRIKIMSNLDITVKRIPQDGKIKFKSVNGDEIELRV
ATIPTQGNVEDVVMRILAKGETLPLEAMGMMDRNYGEMLKILEKPYGMVLVVGPTGSGKTTTLHAALRHINTPERKIWTA
EDPVEITQYGLRQVQVQPKIGFDFAAAMRAFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETITRLL
DMGIDPLNFSDALLCILAQRLVRTLCKKCKEAYHPTRAEYDEIAESYGLEHFARLNLPYTDDFVLYRPKGCDACDRTGYK
GRMGIHELLIGSDSIKRLIQKHADIDTMRETAIVEGMTTLLQDGIRKAIQGMTDFRQVRRVCIK

Sequences:

>Translated_784_residues
MAHLIIYLHTREHGPPCREKVMTDILTKNHQISELEEKLLLRKKLQDITNQIHAARNIKQILVDLKEGILNLFDAYSITI
YVVDRLRNEIFSLLLSASQIKEIRVPINNKSIAGYVANNKKTVNIADAYDVAELRQIDPELSFDLSWDKKTGFRSRQILC
SPVFYNGKLSGVIQILNKKGGGKFSDEEVGFVQEIAEVLGVAFFNQERYARRRKTRYDYLISRDLLTEEELDNAWEEARD
KKEPMESFLMKKYDVSKEDIGKSLSEFYHCKFIQFDDKFPIPGDLLKNLKREYLRRELWVPLCKTDDKIQVIVDDPNNIL
KRDMIESLLKTKAVKYDVAFPEDIIRYINYFFQMPADESSFTELLGKLDEDEETPEEDGEIVTESDSVIMQLVNKIINDA
YFRRSSDIHIEPNVAKKNVDIRFRVDGDCSLYQTVPFNYRAALVSRIKIMSNLDITVKRIPQDGKIKFKSVNGDEIELRV
ATIPTQGNVEDVVMRILAKGETLPLEAMGMMDRNYGEMLKILEKPYGMVLVVGPTGSGKTTTLHAALRHINTPERKIWTA
EDPVEITQYGLRQVQVQPKIGFDFAAAMRAFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETITRLL
DMGIDPLNFSDALLCILAQRLVRTLCKKCKEAYHPTRAEYDEIAESYGLEHFARLNLPYTDDFVLYRPKGCDACDRTGYK
GRMGIHELLIGSDSIKRLIQKHADIDTMRETAIVEGMTTLLQDGIRKAIQGMTDFRQVRRVCIK
>Mature_783_residues
AHLIIYLHTREHGPPCREKVMTDILTKNHQISELEEKLLLRKKLQDITNQIHAARNIKQILVDLKEGILNLFDAYSITIY
VVDRLRNEIFSLLLSASQIKEIRVPINNKSIAGYVANNKKTVNIADAYDVAELRQIDPELSFDLSWDKKTGFRSRQILCS
PVFYNGKLSGVIQILNKKGGGKFSDEEVGFVQEIAEVLGVAFFNQERYARRRKTRYDYLISRDLLTEEELDNAWEEARDK
KEPMESFLMKKYDVSKEDIGKSLSEFYHCKFIQFDDKFPIPGDLLKNLKREYLRRELWVPLCKTDDKIQVIVDDPNNILK
RDMIESLLKTKAVKYDVAFPEDIIRYINYFFQMPADESSFTELLGKLDEDEETPEEDGEIVTESDSVIMQLVNKIINDAY
FRRSSDIHIEPNVAKKNVDIRFRVDGDCSLYQTVPFNYRAALVSRIKIMSNLDITVKRIPQDGKIKFKSVNGDEIELRVA
TIPTQGNVEDVVMRILAKGETLPLEAMGMMDRNYGEMLKILEKPYGMVLVVGPTGSGKTTTLHAALRHINTPERKIWTAE
DPVEITQYGLRQVQVQPKIGFDFAAAMRAFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETITRLLD
MGIDPLNFSDALLCILAQRLVRTLCKKCKEAYHPTRAEYDEIAESYGLEHFARLNLPYTDDFVLYRPKGCDACDRTGYKG
RMGIHELLIGSDSIKRLIQKHADIDTMRETAIVEGMTTLLQDGIRKAIQGMTDFRQVRRVCIK

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Homo sapiens, GI5729972, Length=203, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI194595488, Length=203, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789723, Length=416, Percent_Identity=40.1442307692308, Blast_Score=297, Evalue=2e-81,
Organism=Escherichia coli, GI1786296, Length=401, Percent_Identity=39.1521197007481, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI87082188, Length=152, Percent_Identity=41.4473684210526, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193202333, Length=226, Percent_Identity=26.1061946902655, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 89861; Mature: 89730

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHLIIYLHTREHGPPCREKVMTDILTKNHQISELEEKLLLRKKLQDITNQIHAARNIKQ
CCEEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILVDLKEGILNLFDAYSITIYVVDRLRNEIFSLLLSASQIKEIRVPINNKSIAGYVANNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCC
KTVNIADAYDVAELRQIDPELSFDLSWDKKTGFRSRQILCSPVFYNGKLSGVIQILNKKG
EEEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHCCC
GGKFSDEEVGFVQEIAEVLGVAFFNQERYARRRKTRYDYLISRDLLTEEELDNAWEEARD
CCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
KKEPMESFLMKKYDVSKEDIGKSLSEFYHCKFIQFDDKFPIPGDLLKNLKREYLRRELWV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
PLCKTDDKIQVIVDDPNNILKRDMIESLLKTKAVKYDVAFPEDIIRYINYFFQMPADESS
HHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCCHH
FTELLGKLDEDEETPEEDGEIVTESDSVIMQLVNKIINDAYFRRSSDIHIEPNVAKKNVD
HHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCE
IRFRVDGDCSLYQTVPFNYRAALVSRIKIMSNLDITVKRIPQDGKIKFKSVNGDEIELRV
EEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCEEEEEE
ATIPTQGNVEDVVMRILAKGETLPLEAMGMMDRNYGEMLKILEKPYGMVLVVGPTGSGKT
EECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCCH
TTLHAALRHINTPERKIWTAEDPVEITQYGLRQVQVQPKIGFDFAAAMRAFLRADPDVIM
HHHHHHHHHCCCCCCEEECCCCCHHHHHHCCHHEEECCCCCCHHHHHHHHHHHCCCCEEE
VGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETITRLLDMGIDPLNFSDALLCILAQR
ECCCCCHHHHHCCCCCEEEHEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LVRTLCKKCKEAYHPTRAEYDEIAESYGLEHFARLNLPYTDDFVLYRPKGCDACDRTGYK
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCEEEECCCCCCHHHCCCCC
GRMGIHELLIGSDSIKRLIQKHADIDTMRETAIVEGMTTLLQDGIRKAIQGMTDFRQVRR
CCCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCIK
HHCC
>Mature Secondary Structure 
AHLIIYLHTREHGPPCREKVMTDILTKNHQISELEEKLLLRKKLQDITNQIHAARNIKQ
CEEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILVDLKEGILNLFDAYSITIYVVDRLRNEIFSLLLSASQIKEIRVPINNKSIAGYVANNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCC
KTVNIADAYDVAELRQIDPELSFDLSWDKKTGFRSRQILCSPVFYNGKLSGVIQILNKKG
EEEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHCCC
GGKFSDEEVGFVQEIAEVLGVAFFNQERYARRRKTRYDYLISRDLLTEEELDNAWEEARD
CCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
KKEPMESFLMKKYDVSKEDIGKSLSEFYHCKFIQFDDKFPIPGDLLKNLKREYLRRELWV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
PLCKTDDKIQVIVDDPNNILKRDMIESLLKTKAVKYDVAFPEDIIRYINYFFQMPADESS
HHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCCHH
FTELLGKLDEDEETPEEDGEIVTESDSVIMQLVNKIINDAYFRRSSDIHIEPNVAKKNVD
HHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCE
IRFRVDGDCSLYQTVPFNYRAALVSRIKIMSNLDITVKRIPQDGKIKFKSVNGDEIELRV
EEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCEEEEEE
ATIPTQGNVEDVVMRILAKGETLPLEAMGMMDRNYGEMLKILEKPYGMVLVVGPTGSGKT
EECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCCH
TTLHAALRHINTPERKIWTAEDPVEITQYGLRQVQVQPKIGFDFAAAMRAFLRADPDVIM
HHHHHHHHHCCCCCCEEECCCCCHHHHHHCCHHEEECCCCCCHHHHHHHHHHHCCCCEEE
VGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETITRLLDMGIDPLNFSDALLCILAQR
ECCCCCHHHHHCCCCCEEEHEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LVRTLCKKCKEAYHPTRAEYDEIAESYGLEHFARLNLPYTDDFVLYRPKGCDACDRTGYK
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCEEEECCCCCCHHHCCCCC
GRMGIHELLIGSDSIKRLIQKHADIDTMRETAIVEGMTTLLQDGIRKAIQGMTDFRQVRR
CCCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCIK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]