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The map label for this gene is ispE

Identifier: 85859729

GI number: 85859729

Start: 1958567

End: 1959406

Strand: Direct

Name: ispE

Synonym: SYN_03046

Alternate gene names: 85859729

Gene position: 1958567-1959406 (Clockwise)

Preceding gene: 85859728

Following gene: 85859730

Centisome position: 61.6

GC content: 50.71

Gene sequence:

>840_bases
ATGTTGAAAATATTATCGCCGGCAAAGGTTAACCTTCATCTTCGGGTCTTAAGAAAAAGGGAAGACAACTATCATGATTT
AGCCACACTGATGCAGAAAGTCAGTTTGTACGATGAACTGGAATTCCAGCCATCCAACCGGGGCATCCAACTGCGATGTC
CCGGTTTTTTCCTTCCTGAAAACGAGGAGAACATCGTTTTCAGGGCCGCAAAGAAAATTCTCTCATTCTCCGGCTGCCCC
ACCGGCGTCACCATCACGCTTCGCAAGAACATCCCTCTGGCCGCCGGTCTGGGCGGCGGCAGTTCCAACGCCGCAATGAC
CCTGCTGGCCCTCAACAAATTATTCCGCCTGAACTTCAGTACTGGTGAACTGATGAAAATCGGCGCAACGCTGGGAGCCG
ACGTTCCCTTTTTCCTGTTTCATGGTCCCGCTTGGGCCTACGGCATCGGAGACCGCCTGAAAGCGGCGCTCGATGTCCCT
AAGGTGTGGTTTGTACTGATTAATCCCGGTTTTGAAGTCTCAACCAGAGTGATTTATGAAAAGCTGAATTTAGGATTGAC
AAAAGAAATAATCCATTATAGCATTCCGCGGTTTTTAAGCATGGAGGACATGGCTGCAGGGCTCCATAACGATCTTGAAA
GCGTCACGTTGAAGATGCACCCATCCCTGCAGGAGATCAAGGATCTGCTCTGTTCGTATGGCGCTGCGGGTGCTTTGATG
TCAGGGAGCGGGCCGACCGTTTTCGGGATTTTTGAAAACGAAGACAAGGCAAAAGAGGCGGAAACCGTACTGGGGAAACC
CGGGACATGGGCCGTTTTTTGTGTACGCTCCATCGATTGA

Upstream 100 bases:

>100_bases
AGGATAAGACGAAGGGCAATCTCGATGACCAGGAAAATTCCGTTCTTGAAGAGATTCTCTATGAACTGAAAATGAGGTAT
GTGAAAGAAACATCCTGACC

Downstream 100 bases:

>100_bases
TTTTTTCCCGGTCAATCCTTTAGAGTTCAGCCGATCAGACTGCATCGTCTCTTATCAGCGCGAAATCAAATGGGGCGTCG
TCAAGCGGTAAGACACAAGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MLKILSPAKVNLHLRVLRKREDNYHDLATLMQKVSLYDELEFQPSNRGIQLRCPGFFLPENEENIVFRAAKKILSFSGCP
TGVTITLRKNIPLAAGLGGGSSNAAMTLLALNKLFRLNFSTGELMKIGATLGADVPFFLFHGPAWAYGIGDRLKAALDVP
KVWFVLINPGFEVSTRVIYEKLNLGLTKEIIHYSIPRFLSMEDMAAGLHNDLESVTLKMHPSLQEIKDLLCSYGAAGALM
SGSGPTVFGIFENEDKAKEAETVLGKPGTWAVFCVRSID

Sequences:

>Translated_279_residues
MLKILSPAKVNLHLRVLRKREDNYHDLATLMQKVSLYDELEFQPSNRGIQLRCPGFFLPENEENIVFRAAKKILSFSGCP
TGVTITLRKNIPLAAGLGGGSSNAAMTLLALNKLFRLNFSTGELMKIGATLGADVPFFLFHGPAWAYGIGDRLKAALDVP
KVWFVLINPGFEVSTRVIYEKLNLGLTKEIIHYSIPRFLSMEDMAAGLHNDLESVTLKMHPSLQEIKDLLCSYGAAGALM
SGSGPTVFGIFENEDKAKEAETVLGKPGTWAVFCVRSID
>Mature_279_residues
MLKILSPAKVNLHLRVLRKREDNYHDLATLMQKVSLYDELEFQPSNRGIQLRCPGFFLPENEENIVFRAAKKILSFSGCP
TGVTITLRKNIPLAAGLGGGSSNAAMTLLALNKLFRLNFSTGELMKIGATLGADVPFFLFHGPAWAYGIGDRLKAALDVP
KVWFVLINPGFEVSTRVIYEKLNLGLTKEIIHYSIPRFLSMEDMAAGLHNDLESVTLKMHPSLQEIKDLLCSYGAAGALM
SGSGPTVFGIFENEDKAKEAETVLGKPGTWAVFCVRSID

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=272, Percent_Identity=34.5588235294118, Blast_Score=121, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_SYNAS (Q2LUJ9)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461931.1
- ProteinModelPortal:   Q2LUJ9
- SMR:   Q2LUJ9
- STRING:   Q2LUJ9
- GeneID:   3883604
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_03046
- NMPDR:   fig|56780.10.peg.1875
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- PhylomeDB:   Q2LUJ9
- BioCyc:   SACI56780:SYN_03046-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30689; Mature: 30689

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKILSPAKVNLHLRVLRKREDNYHDLATLMQKVSLYDELEFQPSNRGIQLRCPGFFLPE
CCEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCEECCCCCCEEEECCEEECCC
NEENIVFRAAKKILSFSGCPTGVTITLRKNIPLAAGLGGGSSNAAMTLLALNKLFRLNFS
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHEEECC
TGELMKIGATLGADVPFFLFHGPAWAYGIGDRLKAALDVPKVWFVLINPGFEVSTRVIYE
CCCEEEECHHHCCCCCEEEEECCEEEECCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
KLNLGLTKEIIHYSIPRFLSMEDMAAGLHNDLESVTLKMHPSLQEIKDLLCSYGAAGALM
HHCCCHHHHHHHHHCCHHCCHHHHHHHHHCCHHHEEEEECCCHHHHHHHHHCCCCCCEEE
SGSGPTVFGIFENEDKAKEAETVLGKPGTWAVFCVRSID
CCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MLKILSPAKVNLHLRVLRKREDNYHDLATLMQKVSLYDELEFQPSNRGIQLRCPGFFLPE
CCEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCEECCCCCCEEEECCEEECCC
NEENIVFRAAKKILSFSGCPTGVTITLRKNIPLAAGLGGGSSNAAMTLLALNKLFRLNFS
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHEEECC
TGELMKIGATLGADVPFFLFHGPAWAYGIGDRLKAALDVPKVWFVLINPGFEVSTRVIYE
CCCEEEECHHHCCCCCEEEEECCEEEECCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
KLNLGLTKEIIHYSIPRFLSMEDMAAGLHNDLESVTLKMHPSLQEIKDLLCSYGAAGALM
HHCCCHHHHHHHHHCCHHCCHHHHHHHHHCCHHHEEEEECCCHHHHHHHHHCCCCCCEEE
SGSGPTVFGIFENEDKAKEAETVLGKPGTWAVFCVRSID
CCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA