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The map label for this gene is ykfA [H]

Identifier: 85859683

GI number: 85859683

Start: 1912661

End: 1913527

Strand: Direct

Name: ykfA [H]

Synonym: SYN_01084

Alternate gene names: 85859683

Gene position: 1912661-1913527 (Clockwise)

Preceding gene: 85859682

Following gene: 85859684

Centisome position: 60.16

GC content: 50.52

Gene sequence:

>867_bases
TTGAAGAATGTCTTCATTATCACCCCAAGTTATCTGATCCGGAAAAAGCGCGACTTCAACCGCGGGATCAGACAGCTCTT
TTCACTGGGTTTTAATGTCCTGAATCCGGAATTCCCGATCGCTCCTTCATCGCCGCAAGAAAAAGCGAATCAGATACACA
GGGCTTTTTCCAACCCCGATGTCGATGTGATTCTGGCTCAGAGGGGTGGATACAGCGCAATGAAAACCCTGCCGTTCATC
GATTTCGATTTCCTGAAGAAACATCCCGGAATTCTTGCCGGTTTCAGTGATGTAAGCGCTTTGCTCAATCCCATTTCCGA
GCACACCGGACTGGTTACGCTCCATGCCCCGATGGTAGCCAATCTTGGAACTCCGACAGCCTTTACGGTCAGGTCCTTTA
TGAACGCCGTTAACGGGTGGCCCGAAAAAAACCTCTTCAAAGGCGCCCCGTTGAAGGTCTTCAATCCCGGTTTTGCCAGG
GGCATTTTGAAGGGCGGCAATCTCGTCACCCTGACGGCTCTGATGAATACAAAATGGGAAATCGACACGGATCGATCGAT
TCTTTTTTTTGAAGACGTCGACGAAAAACTCCATCGGGTCGATCGCTGTCTGACCCAGTGGATCCTCGCCGGAAAATTCG
ACGGCGTTCAGGCTGTCATTCTCGGAAATTTCCGTGGAATCAGAAGCAGGCAGGTTTATGAGATTCTTGCCTCACAGATG
GAAATAACGATTCCTGTTATTTCCTGTCCCTGGATCGGCCACGTTCAGAACAAGATCACCCTTCCGGTCGGAGCACAGGT
GGAGTTGAATACGGAGAGGAAGAGTCTCGTTATCACCGAGCTCTCCCTGCCTGGAGTCCGATCATGA

Upstream 100 bases:

>100_bases
TCGATCTGCCAAGATTATCGCACATGATCATACAGGCTGCTGTTGCCCTGATAACGACATGATCTCTCGAGCGTCATTTT
AATTTTCCGAGGTCCTCTTT

Downstream 100 bases:

>100_bases
ACATGATCTGGTTGATCCTGATCTCGGTCAGCATCGTTTTCGCCGTTTTTACAGGAAACTTGGAACCCTTTACCAAAGCG
ATATTCGATGGCGCCAAATC

Product: muramoyltetrapeptide carboxypeptidase

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKNVFIITPSYLIRKKRDFNRGIRQLFSLGFNVLNPEFPIAPSSPQEKANQIHRAFSNPDVDVILAQRGGYSAMKTLPFI
DFDFLKKHPGILAGFSDVSALLNPISEHTGLVTLHAPMVANLGTPTAFTVRSFMNAVNGWPEKNLFKGAPLKVFNPGFAR
GILKGGNLVTLTALMNTKWEIDTDRSILFFEDVDEKLHRVDRCLTQWILAGKFDGVQAVILGNFRGIRSRQVYEILASQM
EITIPVISCPWIGHVQNKITLPVGAQVELNTERKSLVITELSLPGVRS

Sequences:

>Translated_288_residues
MKNVFIITPSYLIRKKRDFNRGIRQLFSLGFNVLNPEFPIAPSSPQEKANQIHRAFSNPDVDVILAQRGGYSAMKTLPFI
DFDFLKKHPGILAGFSDVSALLNPISEHTGLVTLHAPMVANLGTPTAFTVRSFMNAVNGWPEKNLFKGAPLKVFNPGFAR
GILKGGNLVTLTALMNTKWEIDTDRSILFFEDVDEKLHRVDRCLTQWILAGKFDGVQAVILGNFRGIRSRQVYEILASQM
EITIPVISCPWIGHVQNKITLPVGAQVELNTERKSLVITELSLPGVRS
>Mature_288_residues
MKNVFIITPSYLIRKKRDFNRGIRQLFSLGFNVLNPEFPIAPSSPQEKANQIHRAFSNPDVDVILAQRGGYSAMKTLPFI
DFDFLKKHPGILAGFSDVSALLNPISEHTGLVTLHAPMVANLGTPTAFTVRSFMNAVNGWPEKNLFKGAPLKVFNPGFAR
GILKGGNLVTLTALMNTKWEIDTDRSILFFEDVDEKLHRVDRCLTQWILAGKFDGVQAVILGNFRGIRSRQVYEILASQM
EITIPVISCPWIGHVQNKITLPVGAQVELNTERKSLVITELSLPGVRS

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=309, Percent_Identity=32.0388349514563, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 32069; Mature: 32069

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVFIITPSYLIRKKRDFNRGIRQLFSLGFNVLNPEFPIAPSSPQEKANQIHRAFSNPD
CCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
VDVILAQRGGYSAMKTLPFIDFDFLKKHPGILAGFSDVSALLNPISEHTGLVTLHAPMVA
EEEEEECCCCCHHHHHCCCCCHHHHHCCCCEEECHHHHHHHHHHHHHHCCEEEEECCHHH
NLGTPTAFTVRSFMNAVNGWPEKNLFKGAPLKVFNPGFARGILKGGNLVTLTALMNTKWE
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHCCCCEEEEEEECCCCEE
IDTDRSILFFEDVDEKLHRVDRCLTQWILAGKFDGVQAVILGNFRGIRSRQVYEILASQM
ECCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCE
EITIPVISCPWIGHVQNKITLPVGAQVELNTERKSLVITELSLPGVRS
EEEEEEEECCCCCCCCCEEEECCCCEEEECCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MKNVFIITPSYLIRKKRDFNRGIRQLFSLGFNVLNPEFPIAPSSPQEKANQIHRAFSNPD
CCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
VDVILAQRGGYSAMKTLPFIDFDFLKKHPGILAGFSDVSALLNPISEHTGLVTLHAPMVA
EEEEEECCCCCHHHHHCCCCCHHHHHCCCCEEECHHHHHHHHHHHHHHCCEEEEECCHHH
NLGTPTAFTVRSFMNAVNGWPEKNLFKGAPLKVFNPGFARGILKGGNLVTLTALMNTKWE
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHCCCCEEEEEEECCCCEE
IDTDRSILFFEDVDEKLHRVDRCLTQWILAGKFDGVQAVILGNFRGIRSRQVYEILASQM
ECCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCE
EITIPVISCPWIGHVQNKITLPVGAQVELNTERKSLVITELSLPGVRS
EEEEEEEECCCCCCCCCEEEECCCCEEEECCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]