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The map label for this gene is rne [H]

Identifier: 85859580

GI number: 85859580

Start: 1811486

End: 1813378

Strand: Direct

Name: rne [H]

Synonym: SYN_02196

Alternate gene names: 85859580

Gene position: 1811486-1813378 (Clockwise)

Preceding gene: 85859579

Following gene: 85859582

Centisome position: 56.98

GC content: 49.5

Gene sequence:

>1893_bases
ATGAAACATATCATGTTGATCAATGCTGTTCACCATGAACAGAAGCGCATGGCTATCGTTGAAGACGGAAAGCTAGTGGA
GTTTAACATCCAGATGTCTCTGCGGGAACCGATCACAGGGAATATCTATAAAGGGATCGTTCTCAAAGTCGAACGAGGTC
TGCAGGCCGCATTTGTCAATTTCGGCGTCGGCAAGGACGGATTTCTTCCTTTGCGGGATGTAAGTCCTGCATACTTCACA
GAAAAAAAAGAGGCAGGGCGGGAAAGCGCACACTCCAGAGGGTCATTGAAAATCGGACAGGAAGTCATCGTTCAGGTCGC
ACGTGAGGTGAGTGGACGCAAAGGAGCCCTGCTGACCACATACTTATCCCTGCCTGGACGTTACCTTGTTCTGCTGCCCA
ATAAGCACAGCAGTGGAATTTCACGGAAAATCGAAGCTGAAGAAGACCGCCGTCTGTTGAAGACGCTAGTGGAACAGATT
AAAATCGACGAGGGCATGGGGTTCATTGTTCGCACCGCGGGCATAAACCGGACAAAGCAGGAACTGTCCCGGGATTATCA
GCACCTGCTTAGACTGTGGAAGGAAATACAGAAGCGGGCGGAAACCGATCCGGCACCGGCACTGATCTATCAGGAAAGCG
ACTTCGGCGTCAGAAGCCTGAGAGATTACTTCACTTCAGAAATTGAAGAAATACTGGTTGATGATCTCGAGACCTATCGC
AAGATGAGAGCCTACTGCAAGGCTGTTGCGCCGAGAAATCTCCGAATGATCAAGCAGTATAAGGAAAGATTGCCCATTTT
CAGCCAGTACAATCTTGAGGAGCAGATACGAGAGATTTATCAGGAGCGTGTGGAACTGAAGTCCGGGGGGTCGATCGTCA
TCAGTCCGACGGAAGCGATGATCACCATTGATGTCAACTCGGGACGTGCCTCAAATAAGAAAAATGTCGAAGATACGGCT
TTTAAAACAAACCTGGAAGCCGCTGAAGAAATTGGAAGACAGCTTCGACTGAGAGACTTGGGTGGATTGATCGTCATCGA
CTTTATTGACATGACGGATCGGAAGCATGAGGCTGAAGTGGAAAAAACCTTCAAGAAAGCACTTACCCTTGATCGCGCCC
GTATCCAGATGTCCCGGATTTCAAAATTCGGCATCCTGGAATTGTCACGCCAGAAGAAGCATTCCACGATTCAGGAAATC
AGTTATACCACTTGTCCCTTCTGCAGCGGTAGCGGTATCCGCCCCTCCCTGGAATATACGGCACTGAGCGCTTTCCGGAA
AATTGAAACGGAAGCCGCCAAGGAAAAAGCAAACGCCCTGAAAATAAGTGTTCCCTATGAAATTGCTGATTATCTTCTCA
ATCAGAAGCGCACCGACATTCAGAGAATAGAAACTCTGTATGATCTTTCCATTCATATTTCGGGAAAAGCGGATATGACT
TGGGATCATGCGGCCATTTCAGCGGTTGCCCGTGAACTTGCAACCGAAACACCGGAAACGGCGGAGGAAGAAAAGCCCTC
TCTTCCAGTTGAAGCGCTTCGAGGTCAGGAGCTGGAACCTGCCGCGTCGGGAAACGCTGGAGAATCTGTGGAAACAGCTT
CCGGCTCGGGCGGTCCGGAAGAGCTTTTACCTGCAACGGAACTGGCCAAGAAAAAAAGTCGGCGCCGCCCCCGAAAGAGG
AGAAGGAAATCGGAAGAGAAGGTAGAGGCTGCCGCGGCTCCTTCTTTGCCGGAGGGCATGGACGAATCTCCAATTTCCCC
CGAACAAGATGTTTTCGGAGAAATTCTTTCCTATCCACCTGTAATAACTCAAGAAAGCGTTGAAGAACATCCGGTTTTGT
CACCCGTCTCACAAGAATCGGATAACAGGAAGGAAGTCATCGAGGAAGAATAG

Upstream 100 bases:

>100_bases
GTCGAGGAAAAAAACGTTTTTTGATCGTTTGAATCTTTTTTCTCCAGCCCGCAGTCTGCACAGGCGGACACCTTACACAA
AAAGGACTATAAGGACTAAT

Downstream 100 bases:

>100_bases
GTCGTTTAATAATTACAGCCACATTTTATTCATCCCCGGCCAGGACCTTGGAACGCCGGGTGGCGGCCTCAACCGCCGTC
ATCAGAGTCGCTCTCAACCG

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MKHIMLINAVHHEQKRMAIVEDGKLVEFNIQMSLREPITGNIYKGIVLKVERGLQAAFVNFGVGKDGFLPLRDVSPAYFT
EKKEAGRESAHSRGSLKIGQEVIVQVAREVSGRKGALLTTYLSLPGRYLVLLPNKHSSGISRKIEAEEDRRLLKTLVEQI
KIDEGMGFIVRTAGINRTKQELSRDYQHLLRLWKEIQKRAETDPAPALIYQESDFGVRSLRDYFTSEIEEILVDDLETYR
KMRAYCKAVAPRNLRMIKQYKERLPIFSQYNLEEQIREIYQERVELKSGGSIVISPTEAMITIDVNSGRASNKKNVEDTA
FKTNLEAAEEIGRQLRLRDLGGLIVIDFIDMTDRKHEAEVEKTFKKALTLDRARIQMSRISKFGILELSRQKKHSTIQEI
SYTTCPFCSGSGIRPSLEYTALSAFRKIETEAAKEKANALKISVPYEIADYLLNQKRTDIQRIETLYDLSIHISGKADMT
WDHAAISAVARELATETPETAEEEKPSLPVEALRGQELEPAASGNAGESVETASGSGGPEELLPATELAKKKSRRRPRKR
RRKSEEKVEAAAAPSLPEGMDESPISPEQDVFGEILSYPPVITQESVEEHPVLSPVSQESDNRKEVIEEE

Sequences:

>Translated_630_residues
MKHIMLINAVHHEQKRMAIVEDGKLVEFNIQMSLREPITGNIYKGIVLKVERGLQAAFVNFGVGKDGFLPLRDVSPAYFT
EKKEAGRESAHSRGSLKIGQEVIVQVAREVSGRKGALLTTYLSLPGRYLVLLPNKHSSGISRKIEAEEDRRLLKTLVEQI
KIDEGMGFIVRTAGINRTKQELSRDYQHLLRLWKEIQKRAETDPAPALIYQESDFGVRSLRDYFTSEIEEILVDDLETYR
KMRAYCKAVAPRNLRMIKQYKERLPIFSQYNLEEQIREIYQERVELKSGGSIVISPTEAMITIDVNSGRASNKKNVEDTA
FKTNLEAAEEIGRQLRLRDLGGLIVIDFIDMTDRKHEAEVEKTFKKALTLDRARIQMSRISKFGILELSRQKKHSTIQEI
SYTTCPFCSGSGIRPSLEYTALSAFRKIETEAAKEKANALKISVPYEIADYLLNQKRTDIQRIETLYDLSIHISGKADMT
WDHAAISAVARELATETPETAEEEKPSLPVEALRGQELEPAASGNAGESVETASGSGGPEELLPATELAKKKSRRRPRKR
RRKSEEKVEAAAAPSLPEGMDESPISPEQDVFGEILSYPPVITQESVEEHPVLSPVSQESDNRKEVIEEE
>Mature_630_residues
MKHIMLINAVHHEQKRMAIVEDGKLVEFNIQMSLREPITGNIYKGIVLKVERGLQAAFVNFGVGKDGFLPLRDVSPAYFT
EKKEAGRESAHSRGSLKIGQEVIVQVAREVSGRKGALLTTYLSLPGRYLVLLPNKHSSGISRKIEAEEDRRLLKTLVEQI
KIDEGMGFIVRTAGINRTKQELSRDYQHLLRLWKEIQKRAETDPAPALIYQESDFGVRSLRDYFTSEIEEILVDDLETYR
KMRAYCKAVAPRNLRMIKQYKERLPIFSQYNLEEQIREIYQERVELKSGGSIVISPTEAMITIDVNSGRASNKKNVEDTA
FKTNLEAAEEIGRQLRLRDLGGLIVIDFIDMTDRKHEAEVEKTFKKALTLDRARIQMSRISKFGILELSRQKKHSTIQEI
SYTTCPFCSGSGIRPSLEYTALSAFRKIETEAAKEKANALKISVPYEIADYLLNQKRTDIQRIETLYDLSIHISGKADMT
WDHAAISAVARELATETPETAEEEKPSLPVEALRGQELEPAASGNAGESVETASGSGGPEELLPATELAKKKSRRRPRKR
RRKSEEKVEAAAAPSLPEGMDESPISPEQDVFGEILSYPPVITQESVEEHPVLSPVSQESDNRKEVIEEE

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=470, Percent_Identity=47.2340425531915, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI87082235, Length=426, Percent_Identity=36.6197183098592, Blast_Score=259, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 71065; Mature: 71065

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIMLINAVHHEQKRMAIVEDGKLVEFNIQMSLREPITGNIYKGIVLKVERGLQAAFVN
CCCEEEEEHHHHHHHCEEEEECCEEEEEEEEEEECCCCCCCHHHHHHEEECCCHHHHHHC
FGVGKDGFLPLRDVSPAYFTEKKEAGRESAHSRGSLKIGQEVIVQVAREVSGRKGALLTT
CCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEH
YLSLPGRYLVLLPNKHSSGISRKIEAEEDRRLLKTLVEQIKIDEGMGFIVRTAGINRTKQ
EECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
ELSRDYQHLLRLWKEIQKRAETDPAPALIYQESDFGVRSLRDYFTSEIEEILVDDLETYR
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KMRAYCKAVAPRNLRMIKQYKERLPIFSQYNLEEQIREIYQERVELKSGGSIVISPTEAM
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEE
ITIDVNSGRASNKKNVEDTAFKTNLEAAEEIGRQLRLRDLGGLIVIDFIDMTDRKHEAEV
EEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHH
EKTFKKALTLDRARIQMSRISKFGILELSRQKKHSTIQEISYTTCPFCSGSGIRPSLEYT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
ALSAFRKIETEAAKEKANALKISVPYEIADYLLNQKRTDIQRIETLYDLSIHISGKADMT
HHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHEEEEECCCCCCC
WDHAAISAVARELATETPETAEEEKPSLPVEALRGQELEPAASGNAGESVETASGSGGPE
HHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
ELLPATELAKKKSRRRPRKRRRKSEEKVEAAAAPSLPEGMDESPISPEQDVFGEILSYPP
HHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
VITQESVEEHPVLSPVSQESDNRKEVIEEE
CCCCCCHHCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MKHIMLINAVHHEQKRMAIVEDGKLVEFNIQMSLREPITGNIYKGIVLKVERGLQAAFVN
CCCEEEEEHHHHHHHCEEEEECCEEEEEEEEEEECCCCCCCHHHHHHEEECCCHHHHHHC
FGVGKDGFLPLRDVSPAYFTEKKEAGRESAHSRGSLKIGQEVIVQVAREVSGRKGALLTT
CCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEH
YLSLPGRYLVLLPNKHSSGISRKIEAEEDRRLLKTLVEQIKIDEGMGFIVRTAGINRTKQ
EECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
ELSRDYQHLLRLWKEIQKRAETDPAPALIYQESDFGVRSLRDYFTSEIEEILVDDLETYR
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KMRAYCKAVAPRNLRMIKQYKERLPIFSQYNLEEQIREIYQERVELKSGGSIVISPTEAM
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEE
ITIDVNSGRASNKKNVEDTAFKTNLEAAEEIGRQLRLRDLGGLIVIDFIDMTDRKHEAEV
EEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHH
EKTFKKALTLDRARIQMSRISKFGILELSRQKKHSTIQEISYTTCPFCSGSGIRPSLEYT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
ALSAFRKIETEAAKEKANALKISVPYEIADYLLNQKRTDIQRIETLYDLSIHISGKADMT
HHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHEEEEECCCCCCC
WDHAAISAVARELATETPETAEEEKPSLPVEALRGQELEPAASGNAGESVETASGSGGPE
HHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
ELLPATELAKKKSRRRPRKRRRKSEEKVEAAAAPSLPEGMDESPISPEQDVFGEILSYPP
HHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
VITQESVEEHPVLSPVSQESDNRKEVIEEE
CCCCCCHHCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]