Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 85859466

GI number: 85859466

Start: 1671960

End: 1672631

Strand: Direct

Name: purN [H]

Synonym: SYN_00888

Alternate gene names: 85859466

Gene position: 1671960-1672631 (Clockwise)

Preceding gene: 85859465

Following gene: 85859467

Centisome position: 52.59

GC content: 46.88

Gene sequence:

>672_bases
TTGATTATGAACAGAAAGCTGCCTATTGGTGTCCTTGTCTCCGGAAGCGGTTCAAACCTGCAGTCCATCATTGATCACAT
TGAGCGGGGTCTACTTGGTGCGGAGATCAAGGTGGTGATCAGCAACGTACCAGATGCCTATGCCTTGGAACGGGCCCGAA
AACATCACCTTCCCACTCTCGTTATTCGTCATGAAGATTTTGAAACAAGGGAAGCATTCGACGCGGAAATTGTCCGTGTC
TTTAAATCCGCAGATGTTGAGCTTGTCGTTATGGCCGGTTTTATGAGAATTATCACTCCTGTTTTACTTGATGCCTATCC
GTACAGGGTCATGAACATTCACCCTGCCCTTCTGCCTTCATTCCGGGGCATGAACGCGCAGCGTCAGGCTGTCGATTACG
GTGTCCGATTTTCAGGATGCACGGTTCATTTTGTCGACCAAGGCGTTGATTCCGGTCCTATCATCATTCAGGCGGTTGTC
CCCGTTCTTGATGAAGATACGGAAGAAACCCTTTCTGCAAGGATTTTAAAAGAGGAACACCGAATTTATCCCCAAGCCAT
TCAGTTTTTTGTCGAAGGACGAATTTCTGTCAATAATCGCAGGGTAAGAATTCGAGACGGAAAATGTGACGAATTCTTAA
CAGTTCATAACCCTGCCCTTTCCGGATTCTAA

Upstream 100 bases:

>100_bases
GGAACGCCTCAACATGCTGGGGGAAAAGGCCTATATTATCGGATCTATCGAAAAAGCTGACGGCGAGAAAAGCGCTGTTT
CTTTTACATAGCCTGATATA

Downstream 100 bases:

>100_bases
AAAAAGGCAAAAAACTTTATATAATATTATACTATCAATTACATATATAAAGGGCTGTTCTGTTCTTTTTTTTGTGTTAT
ACATAAATTAAAACATATTT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MIMNRKLPIGVLVSGSGSNLQSIIDHIERGLLGAEIKVVISNVPDAYALERARKHHLPTLVIRHEDFETREAFDAEIVRV
FKSADVELVVMAGFMRIITPVLLDAYPYRVMNIHPALLPSFRGMNAQRQAVDYGVRFSGCTVHFVDQGVDSGPIIIQAVV
PVLDEDTEETLSARILKEEHRIYPQAIQFFVEGRISVNNRRVRIRDGKCDEFLTVHNPALSGF

Sequences:

>Translated_223_residues
MIMNRKLPIGVLVSGSGSNLQSIIDHIERGLLGAEIKVVISNVPDAYALERARKHHLPTLVIRHEDFETREAFDAEIVRV
FKSADVELVVMAGFMRIITPVLLDAYPYRVMNIHPALLPSFRGMNAQRQAVDYGVRFSGCTVHFVDQGVDSGPIIIQAVV
PVLDEDTEETLSARILKEEHRIYPQAIQFFVEGRISVNNRRVRIRDGKCDEFLTVHNPALSGF
>Mature_223_residues
MIMNRKLPIGVLVSGSGSNLQSIIDHIERGLLGAEIKVVISNVPDAYALERARKHHLPTLVIRHEDFETREAFDAEIVRV
FKSADVELVVMAGFMRIITPVLLDAYPYRVMNIHPALLPSFRGMNAQRQAVDYGVRFSGCTVHFVDQGVDSGPIIIQAVV
PVLDEDTEETLSARILKEEHRIYPQAIQFFVEGRISVNNRRVRIRDGKCDEFLTVHNPALSGF

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=42.4870466321244, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=42.4870466321244, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=42.4870466321244, Blast_Score=171, Evalue=6e-43,
Organism=Escherichia coli, GI1788846, Length=202, Percent_Identity=40.5940594059406, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1787483, Length=196, Percent_Identity=29.0816326530612, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=183, Percent_Identity=38.7978142076503, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320616, Length=195, Percent_Identity=32.3076923076923, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24582400, Length=195, Percent_Identity=45.6410256410256, Blast_Score=190, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 25058; Mature: 25058

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMNRKLPIGVLVSGSGSNLQSIIDHIERGLLGAEIKVVISNVPDAYALERARKHHLPTL
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCEE
VIRHEDFETREAFDAEIVRVFKSADVELVVMAGFMRIITPVLLDAYPYRVMNIHPALLPS
EEECCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCEEEEECHHHCCH
FRGMNAQRQAVDYGVRFSGCTVHFVDQGVDSGPIIIQAVVPVLDEDTEETLSARILKEEH
HCCCCHHHHHHHCCEEECCEEEEEECCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHH
RIYPQAIQFFVEGRISVNNRRVRIRDGKCDEFLTVHNPALSGF
HHHHHHHHHHHHCEEEECCCEEEEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MIMNRKLPIGVLVSGSGSNLQSIIDHIERGLLGAEIKVVISNVPDAYALERARKHHLPTL
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCEE
VIRHEDFETREAFDAEIVRVFKSADVELVVMAGFMRIITPVLLDAYPYRVMNIHPALLPS
EEECCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCEEEEECHHHCCH
FRGMNAQRQAVDYGVRFSGCTVHFVDQGVDSGPIIIQAVVPVLDEDTEETLSARILKEEH
HCCCCHHHHHHHCCEEECCEEEEEECCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHH
RIYPQAIQFFVEGRISVNNRRVRIRDGKCDEFLTVHNPALSGF
HHHHHHHHHHHHCEEEECCCEEEEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]