Click here to switch to the map view.

The map label for this gene is pfp [H]

Identifier: 85859467

GI number: 85859467

Start: 1672743

End: 1674134

Strand: Direct

Name: pfp [H]

Synonym: SYN_00887

Alternate gene names: 85859467

Gene position: 1672743-1674134 (Clockwise)

Preceding gene: 85859466

Following gene: 85859469

Centisome position: 52.61

GC content: 45.4

Gene sequence:

>1392_bases
TTGCGGCACGGGAGGATTAATGGTTTCATGAAAAAAGTTCAGCAGGAAGATTATATTATACAAAACTTGGGTGAATGTGC
GATCCAGTCTCCGGTAATCGTTAATGATTTTACGCCCGACAGCAAGCGTTTGCTGCTGAAACAATATCTTGACTATAGTA
ATACCGGAAAAAATAAGGACGAAAAAGTGAAAAGGGAGATTCCCCTTTCTGTGGAAGTGGCAGGTCCACGGGAAAAAATA
TTTTTCGATCCTTCCAAAACAAAAGCGGCGATTGTCACTTGCGGCGGCTTGTGCCCGGGAATCAATGACGTGATTCGCGC
CATCGTCATGCAATTATATCACCGTTACGGTGTTCGCAACATAATTGGAATAAAGTATGGATTCCAAGGACTTATACCAT
CATACGGACATGAAATTGTTGAATTGACGCCTGAATCGGTTGAAAATATCCATCGCGAAGGTGGCAGTATATTATCTTCC
TCACGAGGTCAGCAGAATATTGGAGAAATGGTTGACGCCCTTTCGCGAATGAATGTCAATATTTTTTTCTGTATCGGCGG
TGATGGAACCATGCGCGCGGCTGAACGCATTACAGAGGAAATTACAAGACGAAAATTAAGCATAAGCCTAATCGGTATCC
CGAAAACGATCGATAACGATCTGAATTTTGTTCAGAAAACATTCGGGTTTGATACAGCAATCGCTGAATCCGAAAACGCA
ATCTCCTGCGCCCATGTTGAAGCGAAAGGTGCGCCTATGGGAATAGGACTCGTCAAGATTATGGGACGGCTCTCCGGTCA
GATCGCTGTTGGTGCGGCACTGGCACAGAATGACGTCAATTTTGTCCTGATTCCCGAAGTACCTTTTGATCTTGAAGGCG
AAAACGGCCTCTTCAAAGCCTTGGAAAAACGTCTCATACGCAGAAAACATTGTGTTATTCTTGTGGCAGAAGGCGCCGGT
CAGGAACTGATGCGCACAAATAATACGCCTCAGGAAACAGATGCTTCCGGCAATATCCGTCTGCTGGATATCGGCTATTT
TTTAAAGGTGCGGATTGAAGACTACTTTAAAAAAGCGGGCATCCAGATCAATCTGAAATATATTGACCCCAGCTATATGA
TTCGCAGTGTTCGCGCAAGTGCCAGCGACAGTATCTATTGCTCCGCTCTTGGCCAGTACGCCGTTCATGCCGGGATGGCC
GGAAAAACCGGGATGCTGGTCGGTTTATTGAAAGATGAATATGTCCACTTGCCAATCCGGGTTGTTACTTCAGGGAAACA
GATCGATCCGGAAGGCAATTTATGGATGCGGGTTCTTGAATCGACGGGTCAGCCTCCTTCCTTGCTTAATAAACCTTTGG
ACCCTCCTGTTGAGGAACATGTTGTTATTTGA

Upstream 100 bases:

>100_bases
AAACTTTATATAATATTATACTATCAATTACATATATAAAGGGCTGTTCTGTTCTTTTTTTTGTGTTATACATAAATTAA
AACATATTTTAGTCTCCAGC

Downstream 100 bases:

>100_bases
ATTTTTCCATGGGTGAGAAAAGCCCGCCAGTTAAACCTTACCGGCGGGCTAAGCATAAATCCAACACCTATAGAAAAATC
CAGGTTAAATATCTAACTTT

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRHGRINGFMKKVQQEDYIIQNLGECAIQSPVIVNDFTPDSKRLLLKQYLDYSNTGKNKDEKVKREIPLSVEVAGPREKI
FFDPSKTKAAIVTCGGLCPGINDVIRAIVMQLYHRYGVRNIIGIKYGFQGLIPSYGHEIVELTPESVENIHREGGSILSS
SRGQQNIGEMVDALSRMNVNIFFCIGGDGTMRAAERITEEITRRKLSISLIGIPKTIDNDLNFVQKTFGFDTAIAESENA
ISCAHVEAKGAPMGIGLVKIMGRLSGQIAVGAALAQNDVNFVLIPEVPFDLEGENGLFKALEKRLIRRKHCVILVAEGAG
QELMRTNNTPQETDASGNIRLLDIGYFLKVRIEDYFKKAGIQINLKYIDPSYMIRSVRASASDSIYCSALGQYAVHAGMA
GKTGMLVGLLKDEYVHLPIRVVTSGKQIDPEGNLWMRVLESTGQPPSLLNKPLDPPVEEHVVI

Sequences:

>Translated_463_residues
MRHGRINGFMKKVQQEDYIIQNLGECAIQSPVIVNDFTPDSKRLLLKQYLDYSNTGKNKDEKVKREIPLSVEVAGPREKI
FFDPSKTKAAIVTCGGLCPGINDVIRAIVMQLYHRYGVRNIIGIKYGFQGLIPSYGHEIVELTPESVENIHREGGSILSS
SRGQQNIGEMVDALSRMNVNIFFCIGGDGTMRAAERITEEITRRKLSISLIGIPKTIDNDLNFVQKTFGFDTAIAESENA
ISCAHVEAKGAPMGIGLVKIMGRLSGQIAVGAALAQNDVNFVLIPEVPFDLEGENGLFKALEKRLIRRKHCVILVAEGAG
QELMRTNNTPQETDASGNIRLLDIGYFLKVRIEDYFKKAGIQINLKYIDPSYMIRSVRASASDSIYCSALGQYAVHAGMA
GKTGMLVGLLKDEYVHLPIRVVTSGKQIDPEGNLWMRVLESTGQPPSLLNKPLDPPVEEHVVI
>Mature_463_residues
MRHGRINGFMKKVQQEDYIIQNLGECAIQSPVIVNDFTPDSKRLLLKQYLDYSNTGKNKDEKVKREIPLSVEVAGPREKI
FFDPSKTKAAIVTCGGLCPGINDVIRAIVMQLYHRYGVRNIIGIKYGFQGLIPSYGHEIVELTPESVENIHREGGSILSS
SRGQQNIGEMVDALSRMNVNIFFCIGGDGTMRAAERITEEITRRKLSISLIGIPKTIDNDLNFVQKTFGFDTAIAESENA
ISCAHVEAKGAPMGIGLVKIMGRLSGQIAVGAALAQNDVNFVLIPEVPFDLEGENGLFKALEKRLIRRKHCVILVAEGAG
QELMRTNNTPQETDASGNIRLLDIGYFLKVRIEDYFKKAGIQINLKYIDPSYMIRSVRASASDSIYCSALGQYAVHAGMA
GKTGMLVGLLKDEYVHLPIRVVTSGKQIDPEGNLWMRVLESTGQPPSLLNKPLDPPVEEHVVI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=359, Percent_Identity=25.9052924791086, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI266453768, Length=359, Percent_Identity=25.9052924791086, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI266453748, Length=359, Percent_Identity=25.9052924791086, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI4505749, Length=359, Percent_Identity=25.9052924791086, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI11321601, Length=309, Percent_Identity=27.1844660194175, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI48762920, Length=369, Percent_Identity=24.6612466124661, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790350, Length=338, Percent_Identity=27.2189349112426, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25147584, Length=254, Percent_Identity=29.5275590551181, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17558788, Length=299, Percent_Identity=28.0936454849498, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321679, Length=359, Percent_Identity=23.3983286908078, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323861, Length=254, Percent_Identity=26.7716535433071, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28573326, Length=257, Percent_Identity=28.7937743190661, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17647809, Length=261, Percent_Identity=28.735632183908, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652337, Length=257, Percent_Identity=28.7937743190661, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 51087; Mature: 51087

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHGRINGFMKKVQQEDYIIQNLGECAIQSPVIVNDFTPDSKRLLLKQYLDYSNTGKNKD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCHH
EKVKREIPLSVEVAGPREKIFFDPSKTKAAIVTCGGLCPGINDVIRAIVMQLYHRYGVRN
HHHHHHCCEEEEECCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
IIGIKYGFQGLIPSYGHEIVELTPESVENIHREGGSILSSSRGQQNIGEMVDALSRMNVN
EEEEECCCCCCCCCCCCCEEEECHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCCE
IFFCIGGDGTMRAAERITEEITRRKLSISLIGIPKTIDNDLNFVQKTFGFDTAIAESENA
EEEEECCCCHHHHHHHHHHHHHHHEEEEEEEECCCHHCHHHHHHHHHHCCCHHHCCCCCC
ISCAHVEAKGAPMGIGLVKIMGRLSGQIAVGAALAQNDVNFVLIPEVPFDLEGENGLFKA
EEEEEEECCCCCCCHHHHHHHHHHCCCEEEEHHHHCCCCCEEEECCCCCCCCCCCCHHHH
LEKRLIRRKHCVILVAEGAGQELMRTNNTPQETDASGNIRLLDIGYFLKVRIEDYFKKAG
HHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCEEEEEECEEEEEEEHHHHHHCC
IQINLKYIDPSYMIRSVRASASDSIYCSALGQYAVHAGMAGKTGMLVGLLKDEYVHLPIR
CEEEEEEECHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEE
VVTSGKQIDPEGNLWMRVLESTGQPPSLLNKPLDPPVEEHVVI
EEECCCEECCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHCCCC
>Mature Secondary Structure
MRHGRINGFMKKVQQEDYIIQNLGECAIQSPVIVNDFTPDSKRLLLKQYLDYSNTGKNKD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCHH
EKVKREIPLSVEVAGPREKIFFDPSKTKAAIVTCGGLCPGINDVIRAIVMQLYHRYGVRN
HHHHHHCCEEEEECCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
IIGIKYGFQGLIPSYGHEIVELTPESVENIHREGGSILSSSRGQQNIGEMVDALSRMNVN
EEEEECCCCCCCCCCCCCEEEECHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCCE
IFFCIGGDGTMRAAERITEEITRRKLSISLIGIPKTIDNDLNFVQKTFGFDTAIAESENA
EEEEECCCCHHHHHHHHHHHHHHHEEEEEEEECCCHHCHHHHHHHHHHCCCHHHCCCCCC
ISCAHVEAKGAPMGIGLVKIMGRLSGQIAVGAALAQNDVNFVLIPEVPFDLEGENGLFKA
EEEEEEECCCCCCCHHHHHHHHHHCCCEEEEHHHHCCCCCEEEECCCCCCCCCCCCHHHH
LEKRLIRRKHCVILVAEGAGQELMRTNNTPQETDASGNIRLLDIGYFLKVRIEDYFKKAG
HHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCEEEEEECEEEEEEEHHHHHHCC
IQINLKYIDPSYMIRSVRASASDSIYCSALGQYAVHAGMAGKTGMLVGLLKDEYVHLPIR
CEEEEEEECHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEE
VVTSGKQIDPEGNLWMRVLESTGQPPSLLNKPLDPPVEEHVVI
EEECCCEECCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]