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The map label for this gene is proS [H]

Identifier: 85859447

GI number: 85859447

Start: 1651552

End: 1653345

Strand: Direct

Name: proS [H]

Synonym: SYN_00905

Alternate gene names: 85859447

Gene position: 1651552-1653345 (Clockwise)

Preceding gene: 85859446

Following gene: 85859448

Centisome position: 51.95

GC content: 51.56

Gene sequence:

>1794_bases
GTGCCCAACCCGAGCGGCTCGCTGTTTTTATCATTTATTAAAAATCAAGGAGGCCAAATGCGATATTCGGAAATGTTCCT
GCCTACAGAACGGGAAATACCTTCAGATGCAGAGGTTGTCAGTCACCAGCTCATGCTCAGGGCGGGGATGATCAGAAAAT
TGTCAGCAGGAATATACTCCTATCTTCCACTTGGTTACCGGGTTCTTCGCAAGGTGGAACAGATTATCAGAGAGGAAATG
AACCGGGCGGGCGCTCAGGAAGTATATCTGCCTATGGTTCAGCCGGCGGAACTCTGGCAGGAGTCGGGACGCTGGGAACA
TTATGGAAAGGAACTTCTGCGCTTCCGGGATCGGCATGATCGGGAGTATTGTCTGGGTCCCACTCATGAAGAAGTCATTA
CGGATCTGGTCCGTCATGAAATCAAGACGTACCGGCAGCTTCCCAAAAACCTTTACCAGATCCAGACCAAATTCCGGGAT
GAGATTCGTCCCCGTTTCGGAGTGATGCGCGGTCGGGAATTCGGAATGAAAGACGCGTACAGTTTCGATGCCGATGAAGA
AGGAGCAGAAATCAGCTATCAGAAGATGTTTGAAGCCTATAAACGAATCTTCGCCCGCTGCGGTCTTCGCTTTCGTCCAG
TCGAAGCGGATTCGGGGACGATTGGAGGAAGCTTTTCCCACGAATTCATGGTGATGGCCGATTCAGGCGAAGACGGACTG
GTATTCTGCGCAACATGCTCTTATGCGGCCAACCTGGAAAAGGCAGAGGTCCATCCTCCAGAAAAGATGGAAATCTCCAA
TGATGAAATGCTGCTGCTGACGGAGGTTCATACGCCCAATGCGCGGACAATTGACGAAGTCTGTGCGTTTCTTAATGTGA
CGCCGCAGGAGATTGTCAAGACGCTGATTTTCAATGCAGACGGAAATCCTGTAGCTGTTCTGGTTCGCGGAGATGAAGAG
GTCAACGAGATCAAGGTCAAGAATTATCTCAAATGCCAGGAGCTTGAACTGGCCATGGATGACATGATTCAGGACGTCAC
GGGCGCCCCGCGCGGGTTTGCCGGGGCCATCGGCATAAAAAAGGCCAGAATTCTGGCGGATTATTCGCTGCTGAACATGA
AAAACGTCGTCATGGGCGCCAACAAAGAAGACTATCACCTGCGGAATGTCAACGAAGGCCGAGATTTCCAGATATCGGCT
TTTGCGGATCTGAAGGTTGCCAGGGAAACGGATTCCTGCCCACGCTGCCAGGGGGCGCTTAAATTCGCCAGGGGAATCGA
AGTCGGACATGTATTCAAACTGGGAACAAAATACAGCAAGGCCATGGGGGCTTCCTATCTGGATAAAAATGGAAAAGAGC
AGATCATGATCATGGGTTGTTACGGGATTGGCACCGGCCGGACCGTGGCGGCCTGCATTGAACAGAACCACGACGAGAAC
GGCATTGTCTGGCCGATGCCGATCTCCCCTTATCAGGTGATCATCACACCGGTAAACATGAATGATAAGGCCCTCGCCGA
GACAGCGGAACGTCTCTATGAAAGTCTGGCCGGCAAGGGCGCGGAAGTGCTTCTCGATGACCGGGACGAACGGGCCGGCG
TCAAATTCAAGGATGCAGACCTCATCGGAATACCGATCCGAGTGACGGTCGGGCCCAAAAAACTGGCCGAAGGTAAAGTG
GAGGTTCGTTTGCGGGATACAGGCGAGACATCCGATGTCCAGGTGGATTCCGTTGAAAAGATGATCCTTCGCGTCATTGA
AGGAAAAATGAGCGAATCATATTTAATCTTTTGA

Upstream 100 bases:

>100_bases
TAAGAGAGGAAGAATTTGTCAAGGCTTTGCTTCAGGAAATACAGGAGATGACAGGGGAATAACTCGTATATTGAAGTGAG
CGCCGGGATCGGCAGGGTGT

Downstream 100 bases:

>100_bases
ATGTATTGTGAATATCAGAAATATTCGATTCCATACATTCCCATACATCAACAATTGATTGTTATATTATGTATCATTGG
CAAAACCGGCTTCATCAGCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MPNPSGSLFLSFIKNQGGQMRYSEMFLPTEREIPSDAEVVSHQLMLRAGMIRKLSAGIYSYLPLGYRVLRKVEQIIREEM
NRAGAQEVYLPMVQPAELWQESGRWEHYGKELLRFRDRHDREYCLGPTHEEVITDLVRHEIKTYRQLPKNLYQIQTKFRD
EIRPRFGVMRGREFGMKDAYSFDADEEGAEISYQKMFEAYKRIFARCGLRFRPVEADSGTIGGSFSHEFMVMADSGEDGL
VFCATCSYAANLEKAEVHPPEKMEISNDEMLLLTEVHTPNARTIDEVCAFLNVTPQEIVKTLIFNADGNPVAVLVRGDEE
VNEIKVKNYLKCQELELAMDDMIQDVTGAPRGFAGAIGIKKARILADYSLLNMKNVVMGANKEDYHLRNVNEGRDFQISA
FADLKVARETDSCPRCQGALKFARGIEVGHVFKLGTKYSKAMGASYLDKNGKEQIMIMGCYGIGTGRTVAACIEQNHDEN
GIVWPMPISPYQVIITPVNMNDKALAETAERLYESLAGKGAEVLLDDRDERAGVKFKDADLIGIPIRVTVGPKKLAEGKV
EVRLRDTGETSDVQVDSVEKMILRVIEGKMSESYLIF

Sequences:

>Translated_597_residues
MPNPSGSLFLSFIKNQGGQMRYSEMFLPTEREIPSDAEVVSHQLMLRAGMIRKLSAGIYSYLPLGYRVLRKVEQIIREEM
NRAGAQEVYLPMVQPAELWQESGRWEHYGKELLRFRDRHDREYCLGPTHEEVITDLVRHEIKTYRQLPKNLYQIQTKFRD
EIRPRFGVMRGREFGMKDAYSFDADEEGAEISYQKMFEAYKRIFARCGLRFRPVEADSGTIGGSFSHEFMVMADSGEDGL
VFCATCSYAANLEKAEVHPPEKMEISNDEMLLLTEVHTPNARTIDEVCAFLNVTPQEIVKTLIFNADGNPVAVLVRGDEE
VNEIKVKNYLKCQELELAMDDMIQDVTGAPRGFAGAIGIKKARILADYSLLNMKNVVMGANKEDYHLRNVNEGRDFQISA
FADLKVARETDSCPRCQGALKFARGIEVGHVFKLGTKYSKAMGASYLDKNGKEQIMIMGCYGIGTGRTVAACIEQNHDEN
GIVWPMPISPYQVIITPVNMNDKALAETAERLYESLAGKGAEVLLDDRDERAGVKFKDADLIGIPIRVTVGPKKLAEGKV
EVRLRDTGETSDVQVDSVEKMILRVIEGKMSESYLIF
>Mature_596_residues
PNPSGSLFLSFIKNQGGQMRYSEMFLPTEREIPSDAEVVSHQLMLRAGMIRKLSAGIYSYLPLGYRVLRKVEQIIREEMN
RAGAQEVYLPMVQPAELWQESGRWEHYGKELLRFRDRHDREYCLGPTHEEVITDLVRHEIKTYRQLPKNLYQIQTKFRDE
IRPRFGVMRGREFGMKDAYSFDADEEGAEISYQKMFEAYKRIFARCGLRFRPVEADSGTIGGSFSHEFMVMADSGEDGLV
FCATCSYAANLEKAEVHPPEKMEISNDEMLLLTEVHTPNARTIDEVCAFLNVTPQEIVKTLIFNADGNPVAVLVRGDEEV
NEIKVKNYLKCQELELAMDDMIQDVTGAPRGFAGAIGIKKARILADYSLLNMKNVVMGANKEDYHLRNVNEGRDFQISAF
ADLKVARETDSCPRCQGALKFARGIEVGHVFKLGTKYSKAMGASYLDKNGKEQIMIMGCYGIGTGRTVAACIEQNHDENG
IVWPMPISPYQVIITPVNMNDKALAETAERLYESLAGKGAEVLLDDRDERAGVKFKDADLIGIPIRVTVGPKKLAEGKVE
VRLRDTGETSDVQVDSVEKMILRVIEGKMSESYLIF

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=223, Percent_Identity=50.6726457399103, Blast_Score=239, Evalue=5e-63,
Organism=Escherichia coli, GI1786392, Length=576, Percent_Identity=51.2152777777778, Blast_Score=573, Evalue=1e-164,
Organism=Escherichia coli, GI1788013, Length=124, Percent_Identity=28.2258064516129, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115532348, Length=233, Percent_Identity=40.343347639485, Blast_Score=175, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI71991342, Length=204, Percent_Identity=28.4313725490196, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=37.0786516853933, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71991346, Length=176, Percent_Identity=30.1136363636364, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=561, Percent_Identity=32.9768270944742, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6321808, Length=189, Percent_Identity=27.5132275132275, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24656200, Length=214, Percent_Identity=39.2523364485981, Blast_Score=178, Evalue=8e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 67361; Mature: 67230

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNPSGSLFLSFIKNQGGQMRYSEMFLPTEREIPSDAEVVSHQLMLRAGMIRKLSAGIYS
CCCCCHHHHHHHHHCCCCCEEEHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLPLGYRVLRKVEQIIREEMNRAGAQEVYLPMVQPAELWQESGRWEHYGKELLRFRDRHD
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCC
REYCLGPTHEEVITDLVRHEIKTYRQLPKNLYQIQTKFRDEIRPRFGVMRGREFGMKDAY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCC
SFDADEEGAEISYQKMFEAYKRIFARCGLRFRPVEADSGTIGGSFSHEFMVMADSGEDGL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCE
VFCATCSYAANLEKAEVHPPEKMEISNDEMLLLTEVHTPNARTIDEVCAFLNVTPQEIVK
EEEEECCHHCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCHHHHHHHHCCCHHHHHH
TLIFNADGNPVAVLVRGDEEVNEIKVKNYLKCQELELAMDDMIQDVTGAPRGFAGAIGIK
HHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCH
KARILADYSLLNMKNVVMGANKEDYHLRNVNEGRDFQISAFADLKVARETDSCPRCQGAL
HHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCEEEEEEECEEEECCCCCCCHHHHHH
KFARGIEVGHVFKLGTKYSKAMGASYLDKNGKEQIMIMGCYGIGTGRTVAACIEQNHDEN
HHHHCCCCCCEEECCCHHHHHHCCHHHCCCCCCEEEEEEEEECCCCCHHHHHHHCCCCCC
GIVWPMPISPYQVIITPVNMNDKALAETAERLYESLAGKGAEVLLDDRDERAGVKFKDAD
CEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCC
LIGIPIRVTVGPKKLAEGKVEVRLRDTGETSDVQVDSVEKMILRVIEGKMSESYLIF
EEEEEEEEEECHHHHCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure 
PNPSGSLFLSFIKNQGGQMRYSEMFLPTEREIPSDAEVVSHQLMLRAGMIRKLSAGIYS
CCCCHHHHHHHHHCCCCCEEEHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLPLGYRVLRKVEQIIREEMNRAGAQEVYLPMVQPAELWQESGRWEHYGKELLRFRDRHD
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCC
REYCLGPTHEEVITDLVRHEIKTYRQLPKNLYQIQTKFRDEIRPRFGVMRGREFGMKDAY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCC
SFDADEEGAEISYQKMFEAYKRIFARCGLRFRPVEADSGTIGGSFSHEFMVMADSGEDGL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCE
VFCATCSYAANLEKAEVHPPEKMEISNDEMLLLTEVHTPNARTIDEVCAFLNVTPQEIVK
EEEEECCHHCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCHHHHHHHHCCCHHHHHH
TLIFNADGNPVAVLVRGDEEVNEIKVKNYLKCQELELAMDDMIQDVTGAPRGFAGAIGIK
HHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCH
KARILADYSLLNMKNVVMGANKEDYHLRNVNEGRDFQISAFADLKVARETDSCPRCQGAL
HHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCEEEEEEECEEEECCCCCCCHHHHHH
KFARGIEVGHVFKLGTKYSKAMGASYLDKNGKEQIMIMGCYGIGTGRTVAACIEQNHDEN
HHHHCCCCCCEEECCCHHHHHHCCHHHCCCCCCEEEEEEEEECCCCCHHHHHHHCCCCCC
GIVWPMPISPYQVIITPVNMNDKALAETAERLYESLAGKGAEVLLDDRDERAGVKFKDAD
CEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCC
LIGIPIRVTVGPKKLAEGKVEVRLRDTGETSDVQVDSVEKMILRVIEGKMSESYLIF
EEEEEEEEEECHHHHCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA