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The map label for this gene is ispG [H]

Identifier: 85859446

GI number: 85859446

Start: 1650446

End: 1651513

Strand: Direct

Name: ispG [H]

Synonym: SYN_00906

Alternate gene names: 85859446

Gene position: 1650446-1651513 (Clockwise)

Preceding gene: 85859445

Following gene: 85859447

Centisome position: 51.91

GC content: 51.97

Gene sequence:

>1068_bases
TTGAATAACCAGGTCAAGAGAAGAAAGACCAAAGCGGTCAATGTCGGAAAGGTTCAGGTTGGTGGAGGGGCCCCGGTTGC
CGTTCAGTCCATGACCTGCACGGATACACGCAACGTCCGCGAAACGATAGAGCAGATTGAAGCCCTGGAAAAGGCCGGCT
GTGAAATTGTCCGGGTGGCAGTGCCTGATGCTGAAGCCGCTTCCGTATTGGGAAAAATAAAAAAGTCCATTTCCATTCCC
CTGATTGCCGATATCCATTTCAATCATCATTATGCCCTCATGGCCATCGAGCAGGATGTTGACGGGATTCGGATCAATCC
GGGAAATGTGGGTAAAAAGAATATTGCGGAAATTGTAAGGGCTGCAAAGGATCACAATACGGTCATCCGGATCGGTGTGA
ATGCCGGATCGCTCGAAAAGGAACTGCAGGAAAAGCACGGCGGCCCAACAGCCCATGCGCTGACAGAAAGCGCCTTGAAC
AATCTTGCCTTTGTTGAGGATATGGGTTTTGACCGGATCAAACTCTCCCTCAAATCATCCCATGTTCCCACCATGATTGA
CGCTTACCGGCTTGTCGCGGATAAAACCGACTATCCCCTTCACCTGGGTGTGACCGAAGCGGGCACTCTTGTCACTTCCG
CTGTCAAGTCCTCCATCGGCATGGGACTTCTCCTGCACGAAGGGATAGGCGATACCATCCGTGTTTCAGTCACCGGCAGT
CCGGTTTTGGAAATTGGAATCGCTTACGACATTCTGCGCGCCCTGGATATTCGCAGAGTAGGGCCGGATATCATTTCCTG
TCCCACCTGCGGACGCTGTGAGATCGATCTTTTTGGCCTGGTTGAGCAGGTCGAGCACTCCTTGTCCGGAATGAAAGATT
ACATGAAGATAGCCCTCATGGGATGTGTCGTGAACGGTCCAGGCGAGGCGGCTGAAGCTGATGTCGGCATTGCGGGCGGC
AGGGGAATGGGCCTGCTTTTCAAAAAGGGGCAGGTGATCCGTAAGATAAGAGAGGAAGAATTTGTCAAGGCTTTGCTTCA
GGAAATACAGGAGATGACAGGGGAATAA

Upstream 100 bases:

>100_bases
CGTAATCATTTCATTCCAGGAACTTCGGATATTCCTCATTGAAGGTGTTTATGCCTTTGTGCTATACCGATTAGCAAATA
TCAAAGTATAAGGAATTTCA

Downstream 100 bases:

>100_bases
CTCGTATATTGAAGTGAGCGCCGGGATCGGCAGGGTGTGTGCCCAACCCGAGCGGCTCGCTGTTTTTATCATTTATTAAA
AATCAAGGAGGCCAAATGCG

Product: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MNNQVKRRKTKAVNVGKVQVGGGAPVAVQSMTCTDTRNVRETIEQIEALEKAGCEIVRVAVPDAEAASVLGKIKKSISIP
LIADIHFNHHYALMAIEQDVDGIRINPGNVGKKNIAEIVRAAKDHNTVIRIGVNAGSLEKELQEKHGGPTAHALTESALN
NLAFVEDMGFDRIKLSLKSSHVPTMIDAYRLVADKTDYPLHLGVTEAGTLVTSAVKSSIGMGLLLHEGIGDTIRVSVTGS
PVLEIGIAYDILRALDIRRVGPDIISCPTCGRCEIDLFGLVEQVEHSLSGMKDYMKIALMGCVVNGPGEAAEADVGIAGG
RGMGLLFKKGQVIRKIREEEFVKALLQEIQEMTGE

Sequences:

>Translated_355_residues
MNNQVKRRKTKAVNVGKVQVGGGAPVAVQSMTCTDTRNVRETIEQIEALEKAGCEIVRVAVPDAEAASVLGKIKKSISIP
LIADIHFNHHYALMAIEQDVDGIRINPGNVGKKNIAEIVRAAKDHNTVIRIGVNAGSLEKELQEKHGGPTAHALTESALN
NLAFVEDMGFDRIKLSLKSSHVPTMIDAYRLVADKTDYPLHLGVTEAGTLVTSAVKSSIGMGLLLHEGIGDTIRVSVTGS
PVLEIGIAYDILRALDIRRVGPDIISCPTCGRCEIDLFGLVEQVEHSLSGMKDYMKIALMGCVVNGPGEAAEADVGIAGG
RGMGLLFKKGQVIRKIREEEFVKALLQEIQEMTGE
>Mature_355_residues
MNNQVKRRKTKAVNVGKVQVGGGAPVAVQSMTCTDTRNVRETIEQIEALEKAGCEIVRVAVPDAEAASVLGKIKKSISIP
LIADIHFNHHYALMAIEQDVDGIRINPGNVGKKNIAEIVRAAKDHNTVIRIGVNAGSLEKELQEKHGGPTAHALTESALN
NLAFVEDMGFDRIKLSLKSSHVPTMIDAYRLVADKTDYPLHLGVTEAGTLVTSAVKSSIGMGLLLHEGIGDTIRVSVTGS
PVLEIGIAYDILRALDIRRVGPDIISCPTCGRCEIDLFGLVEQVEHSLSGMKDYMKIALMGCVVNGPGEAAEADVGIAGG
RGMGLLFKKGQVIRKIREEEFVKALLQEIQEMTGE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=340, Percent_Identity=49.7058823529412, Blast_Score=332, Evalue=3e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 38102; Mature: 38102

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQVKRRKTKAVNVGKVQVGGGAPVAVQSMTCTDTRNVRETIEQIEALEKAGCEIVRVA
CCCHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEE
VPDAEAASVLGKIKKSISIPLIADIHFNHHYALMAIEQDVDGIRINPGNVGKKNIAEIVR
CCCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEECCCCCEEECCCCCCHHHHHHHHH
AAKDHNTVIRIGVNAGSLEKELQEKHGGPTAHALTESALNNLAFVEDMGFDRIKLSLKSS
HHCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCEEEEEECCC
HVPTMIDAYRLVADKTDYPLHLGVTEAGTLVTSAVKSSIGMGLLLHEGIGDTIRVSVTGS
CCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCC
PVLEIGIAYDILRALDIRRVGPDIISCPTCGRCEIDLFGLVEQVEHSLSGMKDYMKIALM
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GCVVNGPGEAAEADVGIAGGRGMGLLFKKGQVIRKIREEEFVKALLQEIQEMTGE
HEEECCCCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNQVKRRKTKAVNVGKVQVGGGAPVAVQSMTCTDTRNVRETIEQIEALEKAGCEIVRVA
CCCHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEE
VPDAEAASVLGKIKKSISIPLIADIHFNHHYALMAIEQDVDGIRINPGNVGKKNIAEIVR
CCCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEECCCCCEEECCCCCCHHHHHHHHH
AAKDHNTVIRIGVNAGSLEKELQEKHGGPTAHALTESALNNLAFVEDMGFDRIKLSLKSS
HHCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCEEEEEECCC
HVPTMIDAYRLVADKTDYPLHLGVTEAGTLVTSAVKSSIGMGLLLHEGIGDTIRVSVTGS
CCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCC
PVLEIGIAYDILRALDIRRVGPDIISCPTCGRCEIDLFGLVEQVEHSLSGMKDYMKIALM
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GCVVNGPGEAAEADVGIAGGRGMGLLFKKGQVIRKIREEEFVKALLQEIQEMTGE
HEEECCCCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA