Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 85859445

GI number: 85859445

Start: 1648771

End: 1650168

Strand: Direct

Name: purF [H]

Synonym: SYN_00907

Alternate gene names: 85859445

Gene position: 1648771-1650168 (Clockwise)

Preceding gene: 85859444

Following gene: 85859446

Centisome position: 51.86

GC content: 51.86

Gene sequence:

>1398_bases
ATGGGCGGATTATTCGGGGTTGTTTCCAAGGGAAACTGCATAAAGCCTCTTTTTTATGGAACAGATTATCATTCCCACCT
GGGAACGGAAAACGGGGGGCTTGCCGTAATCGGAGAAAAAAGTTTTCATAAAGCCATACACAGCATCAGTCAGGCGCAAT
TCAAATCGAAATTTATCGATCATTACCGTCAGATGAGAGGGACGGCCGGAATCGGAGCCATTGACGATGAATCGCCGCAG
CCTTTGATTATCCGCTCCAAATTCGGCACCTTTGCGATTGCTGCTTCGGGTCTGGTCACCAATAAGGATCGTCTCGCTCA
TGAACTGCTTCAAGAGGGAACCTCATTCAGTGAAGTGGCGGGAGGCGCCGTCAACACAGTCGATCTAGTGGCAAAGCTGA
TCAGCCGGAGTACAAGCCTCGTCGAGGGCATTGTGCAGATGCAGGAAGTCATCGAGGGCTCAATCTGTGTTCTGATTTTG
ACATCGGAAGGCCTCTATGCCGCGAGAGACAAATATGGCCGATTTCCTCTGGCATTGGGAAGGGAAAGAGATGGAGACGG
GTACGCCGTAGCGACGGAATCCAGCTCGTTTCCCAATCTGAACTATGAACTGGTGAAATTTATCGGTCCGGGAGAAATTG
TTCTGATGACCGCCGACGGCTACAGGACCTTGCGGCCTGAGAATCCGGAAAAGAAAGTCTGCGCGTTCCTCTGGATTTAT
ACCGGCACGCCTTCCTCCTCTTATGAAGGAATTTCAGTGGAAAACGCAAGGGAACACTGTGGGCGGGCTCTGGCTAGAGA
GGACAGCATTGCGGCTGACCTGGTCTGCGGCATACCGGATTCAGGCGTCGGGCATGCCCTGGGATATGCAGCGGAATCAC
GAATTCCCTATCGCCGACCTCTGGTCAAATACACGCCCGGCTATGGTCGGAGTTATACGCCTCCCACTCAGGATATCCGT
GATCTTGTGGCCACCATGAAACTGAGTGCCATTCGTGATGTCATCAAAGACAACCGGATGATCGTCTGCGATGATTCGAT
TGTACGAGGCACGCAGTTGAAAAATTACACCATAAAGAAACTGTGGGATAACGGCGCTCGAGAGATTCATATTCGGGCCG
CCTGTCCGCCACTCATGTTTACATGCCCTTATGGATCATCAACACGCTCTCTTTCGGAGCTGGCTGCCAGACGCGCCATC
CGGGCCATCGAGGGAAAGGATCCTGATGACGTTGCACCGTATCTTGATTGCCGTTCGGCGGAATATGCCAGAATGGTCGA
ATGGATCCGCAAAGATTTGAACGTGACGACTTTGAAATACCTCAATATTGAATCCATGATCGAAGCCATCGGCCTGTCGA
AAGATCAGCTGTGCCTTCACTGCTGGCGGGGGCACTGA

Upstream 100 bases:

>100_bases
GGGCTGGAACGAATACGGATGGCCTTGAAACGGTTAAGTTGACCATAAATTTGAATGATTAAGTTTAAAAAAATTATAAA
ATTTTACAGGTAAACATGCC

Downstream 100 bases:

>100_bases
TCTTTGCTCTACAGAAGGCCTTCCACAGAACCCTTCATTCATGGCAAGAAAAGGCATGTCCTGCGCTCCTGTTGAATGGA
AACCCTCTGAGTCAACCTGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGGLFGVVSKGNCIKPLFYGTDYHSHLGTENGGLAVIGEKSFHKAIHSISQAQFKSKFIDHYRQMRGTAGIGAIDDESPQ
PLIIRSKFGTFAIAASGLVTNKDRLAHELLQEGTSFSEVAGGAVNTVDLVAKLISRSTSLVEGIVQMQEVIEGSICVLIL
TSEGLYAARDKYGRFPLALGRERDGDGYAVATESSSFPNLNYELVKFIGPGEIVLMTADGYRTLRPENPEKKVCAFLWIY
TGTPSSSYEGISVENAREHCGRALAREDSIAADLVCGIPDSGVGHALGYAAESRIPYRRPLVKYTPGYGRSYTPPTQDIR
DLVATMKLSAIRDVIKDNRMIVCDDSIVRGTQLKNYTIKKLWDNGAREIHIRAACPPLMFTCPYGSSTRSLSELAARRAI
RAIEGKDPDDVAPYLDCRSAEYARMVEWIRKDLNVTTLKYLNIESMIEAIGLSKDQLCLHCWRGH

Sequences:

>Translated_465_residues
MGGLFGVVSKGNCIKPLFYGTDYHSHLGTENGGLAVIGEKSFHKAIHSISQAQFKSKFIDHYRQMRGTAGIGAIDDESPQ
PLIIRSKFGTFAIAASGLVTNKDRLAHELLQEGTSFSEVAGGAVNTVDLVAKLISRSTSLVEGIVQMQEVIEGSICVLIL
TSEGLYAARDKYGRFPLALGRERDGDGYAVATESSSFPNLNYELVKFIGPGEIVLMTADGYRTLRPENPEKKVCAFLWIY
TGTPSSSYEGISVENAREHCGRALAREDSIAADLVCGIPDSGVGHALGYAAESRIPYRRPLVKYTPGYGRSYTPPTQDIR
DLVATMKLSAIRDVIKDNRMIVCDDSIVRGTQLKNYTIKKLWDNGAREIHIRAACPPLMFTCPYGSSTRSLSELAARRAI
RAIEGKDPDDVAPYLDCRSAEYARMVEWIRKDLNVTTLKYLNIESMIEAIGLSKDQLCLHCWRGH
>Mature_464_residues
GGLFGVVSKGNCIKPLFYGTDYHSHLGTENGGLAVIGEKSFHKAIHSISQAQFKSKFIDHYRQMRGTAGIGAIDDESPQP
LIIRSKFGTFAIAASGLVTNKDRLAHELLQEGTSFSEVAGGAVNTVDLVAKLISRSTSLVEGIVQMQEVIEGSICVLILT
SEGLYAARDKYGRFPLALGRERDGDGYAVATESSSFPNLNYELVKFIGPGEIVLMTADGYRTLRPENPEKKVCAFLWIYT
GTPSSSYEGISVENAREHCGRALAREDSIAADLVCGIPDSGVGHALGYAAESRIPYRRPLVKYTPGYGRSYTPPTQDIRD
LVATMKLSAIRDVIKDNRMIVCDDSIVRGTQLKNYTIKKLWDNGAREIHIRAACPPLMFTCPYGSSTRSLSELAARRAIR
AIEGKDPDDVAPYLDCRSAEYARMVEWIRKDLNVTTLKYLNIESMIEAIGLSKDQLCLHCWRGH

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=460, Percent_Identity=28.0434782608696, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1788651, Length=488, Percent_Identity=27.2540983606557, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17554892, Length=441, Percent_Identity=30.6122448979592, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323958, Length=472, Percent_Identity=23.9406779661017, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24659604, Length=481, Percent_Identity=28.4823284823285, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24659598, Length=481, Percent_Identity=28.2744282744283, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI28573187, Length=510, Percent_Identity=28.4313725490196, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51102; Mature: 50971

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGLFGVVSKGNCIKPLFYGTDYHSHLGTENGGLAVIGEKSFHKAIHSISQAQFKSKFID
CCCCEEECCCCCEECEEEECCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
HYRQMRGTAGIGAIDDESPQPLIIRSKFGTFAIAASGLVTNKDRLAHELLQEGTSFSEVA
HHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHC
GGAVNTVDLVAKLISRSTSLVEGIVQMQEVIEGSICVLILTSEGLYAARDKYGRFPLALG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHCCCCCEECC
RERDGDGYAVATESSSFPNLNYELVKFIGPGEIVLMTADGYRTLRPENPEKKVCAFLWIY
CCCCCCCEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEEEE
TGTPSSSYEGISVENAREHCGRALAREDSIAADLVCGIPDSGVGHALGYAAESRIPYRRP
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEECCCCCCCCHHHHHHHHCCCCCCCC
LVKYTPGYGRSYTPPTQDIRDLVATMKLSAIRDVIKDNRMIVCDDSIVRGTQLKNYTIKK
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHH
LWDNGAREIHIRAACPPLMFTCPYGSSTRSLSELAARRAIRAIEGKDPDDVAPYLDCRSA
HHCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCH
EYARMVEWIRKDLNVTTLKYLNIESMIEAIGLSKDQLCLHCWRGH
HHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCHHHHHEECCC
>Mature Secondary Structure 
GGLFGVVSKGNCIKPLFYGTDYHSHLGTENGGLAVIGEKSFHKAIHSISQAQFKSKFID
CCCEEECCCCCEECEEEECCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
HYRQMRGTAGIGAIDDESPQPLIIRSKFGTFAIAASGLVTNKDRLAHELLQEGTSFSEVA
HHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHC
GGAVNTVDLVAKLISRSTSLVEGIVQMQEVIEGSICVLILTSEGLYAARDKYGRFPLALG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHCCCCCEECC
RERDGDGYAVATESSSFPNLNYELVKFIGPGEIVLMTADGYRTLRPENPEKKVCAFLWIY
CCCCCCCEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEEEE
TGTPSSSYEGISVENAREHCGRALAREDSIAADLVCGIPDSGVGHALGYAAESRIPYRRP
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEECCCCCCCCHHHHHHHHCCCCCCCC
LVKYTPGYGRSYTPPTQDIRDLVATMKLSAIRDVIKDNRMIVCDDSIVRGTQLKNYTIKK
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHH
LWDNGAREIHIRAACPPLMFTCPYGSSTRSLSELAARRAIRAIEGKDPDDVAPYLDCRSA
HHCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCH
EYARMVEWIRKDLNVTTLKYLNIESMIEAIGLSKDQLCLHCWRGH
HHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]