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The map label for this gene is pycB [H]

Identifier: 85859419

GI number: 85859419

Start: 1625292

End: 1627253

Strand: Direct

Name: pycB [H]

Synonym: SYN_01040

Alternate gene names: 85859419

Gene position: 1625292-1627253 (Clockwise)

Preceding gene: 85859418

Following gene: 85859420

Centisome position: 51.12

GC content: 50.41

Gene sequence:

>1962_bases
ATGAAAATTAAAGTATATCCTATGAAGATATCCGAACATCTCCTTGAAGAACGAATTTCACCCCAAGACTTAAAGAAAAA
AGGCGTTTCCTTTATTGTTGATAAAATCCGGCGATCCGAGGGGTATTACGTGACCAACACGGAGCGGGACCTCTCCCAGT
CCGATTTCAAGAACCGGGTCATGCCCCATACACAGCTTCTCGTAGCCAGGGAACGAAATGAAGCAGGCTTTTTTTCCATT
GAGATTACAGGCGGCGCCTCGATTCACGTTGACATGCTGCGCAAACAGATTAATCCCCTGGAAAAGCTGGAAGTATTAAG
CGCCAACATGCCGGATACGCTTTTTCAGACCCTGTGCCGGGGGATAAACATGTTTGGATACCGGCCCTATCCAGAAAACG
TGATCCGTTTGACGGTTCGATCCTTTGCCCGATACGTCCATATATGGAGGGTGTTCGATTTCCTGAACCACGTTTCGAAC
ATGATCCCTGTGTTTGAAGAAGTGAGGGCGTCGGGCTGCCTTCTGGAGCCGGCCATCTGCTTTTCCACGGGTCCCGAACA
CACGGACGCCTATTATGTAAAAAAAGTCGGAGAGATCCTCGATGTTACCGGACCCGATATCCTGTTGGCCATCAAAAACC
ATGGAGGACTGGGGACACCCAAACGGATCGGCGAACTGGTGCGATCCATATTGAATGCCTACCCCGATCTGATTATCCAT
TATCACGGACACAACACGGATGGCGCGGATGTCGGCAGGATTGTAGAGGCTGTGCGGAACGGCGCAAAGATCGTCGATGC
CGCGGACCATGCTTTTACCGGTTTTTACGGACCGCCTCCGCTGCTGACCGTTGTGGATGTCCTGCAGGAATATGGATACC
GGCCATCCGGACTGAATCGTCAGGCAGTTATGGATACCTCAAATAAACTTCGACCGGAACGTGAATACTATAAGGACTTT
GAGTCTCAGTTTCTTGGGTTTGATTCCACGGTCCAGATTCACAAACTGCCCGGGGGGGCAACCGGTTCGAGCTTTGAGCA
GGCCGTGAAGGGGGGATTCCTGAACCGCATGCCGGAGATCCTGCAGAACGAACTGCCCCGCGTTCATGTTGAACTGGGGA
ACTGGTGGAGCGTCACGCCCGGGTCTCAAATTCTCTGGACCACCGCCGTCAATAATGTCCTTAAAGGGCAACGGTATAAG
GAAGTCAATGACGACCTGAAAAATCTCATGCTGGGGCGTTACGGAGAACTTCCTTTTTATCGCCCTTCGGATGAAATTTA
TTGTTCGGTCTTTGGACCTGAATGGAAAAAAATAGTTGAGCAGGAATACGGCTTTCAGAAGGTGGAGGATATCGACATCG
AAGTCGAGAAAAAGGTGCTGGAACACCGGTTGGGACGCGAAGCGACGGAAGACGAACTCGTCCTTTACCTGCAGCATCCC
AATGACGCGGTGGATTTTTTCAAATTCGAAGCCAAATACGGCAAAACCTGGGTGCTGCCGCCAAAGATATGGTTTAAAAA
GGGCGGCTTCAATCTGGGTGAAAAGTTCGAAATTCTCGATGCATTCGGAAAACTGCATATCATTGAAATCGGAACCCAGC
GGAAAACAAAGACCGGTGACGCGATTACTTACATGCTCATCGATCACCATTCACAGCCCATCCTGACCGAATTGGAAGGT
GAGGGACCGTCAGCGGCAAGAAAGATCCAGTTGACAGCCAAAGAGATCGACGCCCTGGCTCTGTCCGGCGACATCCGTTC
ACACATTACCGGTACAGTGAGCGAAATTCCTGTTTCGGAGGGAGATGAAGTGTCCGCGGGCCAGATCCTCATCATCCTTG
AGGCGATGAAGATGTTGAACAATGTTGTTTCTGAAGTCAACGGCCAGGTTTCGGAAATTTTTGTTTCCCCTGGAGACAGG
GTTGAAGTCGGCGCGCCGCTGCTGATGATAAAAAAAGCATAA

Upstream 100 bases:

>100_bases
AAATCGTCCGGCCGCCCGCCGATGACGCTTCGCAGAAAATTCAAGCCATTCCCGGCATTTTCTCTCTGATGGCGAGGAGG
GGCTTTAACCTAAACTTAAA

Downstream 100 bases:

>100_bases
TGCTTTCAGGTTAATCGCTTCTTTATCGAATGGGAAAAAGAAGAGGAGCGGGAACCCGGTTTGTTCAGATTATGATATTA
AGATTATGATGTTAAGTACA

Product: pyruvate carboxylase carboxyltransferase subunit

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 653; Mature: 653

Protein sequence:

>653_residues
MKIKVYPMKISEHLLEERISPQDLKKKGVSFIVDKIRRSEGYYVTNTERDLSQSDFKNRVMPHTQLLVARERNEAGFFSI
EITGGASIHVDMLRKQINPLEKLEVLSANMPDTLFQTLCRGINMFGYRPYPENVIRLTVRSFARYVHIWRVFDFLNHVSN
MIPVFEEVRASGCLLEPAICFSTGPEHTDAYYVKKVGEILDVTGPDILLAIKNHGGLGTPKRIGELVRSILNAYPDLIIH
YHGHNTDGADVGRIVEAVRNGAKIVDAADHAFTGFYGPPPLLTVVDVLQEYGYRPSGLNRQAVMDTSNKLRPEREYYKDF
ESQFLGFDSTVQIHKLPGGATGSSFEQAVKGGFLNRMPEILQNELPRVHVELGNWWSVTPGSQILWTTAVNNVLKGQRYK
EVNDDLKNLMLGRYGELPFYRPSDEIYCSVFGPEWKKIVEQEYGFQKVEDIDIEVEKKVLEHRLGREATEDELVLYLQHP
NDAVDFFKFEAKYGKTWVLPPKIWFKKGGFNLGEKFEILDAFGKLHIIEIGTQRKTKTGDAITYMLIDHHSQPILTELEG
EGPSAARKIQLTAKEIDALALSGDIRSHITGTVSEIPVSEGDEVSAGQILIILEAMKMLNNVVSEVNGQVSEIFVSPGDR
VEVGAPLLMIKKA

Sequences:

>Translated_653_residues
MKIKVYPMKISEHLLEERISPQDLKKKGVSFIVDKIRRSEGYYVTNTERDLSQSDFKNRVMPHTQLLVARERNEAGFFSI
EITGGASIHVDMLRKQINPLEKLEVLSANMPDTLFQTLCRGINMFGYRPYPENVIRLTVRSFARYVHIWRVFDFLNHVSN
MIPVFEEVRASGCLLEPAICFSTGPEHTDAYYVKKVGEILDVTGPDILLAIKNHGGLGTPKRIGELVRSILNAYPDLIIH
YHGHNTDGADVGRIVEAVRNGAKIVDAADHAFTGFYGPPPLLTVVDVLQEYGYRPSGLNRQAVMDTSNKLRPEREYYKDF
ESQFLGFDSTVQIHKLPGGATGSSFEQAVKGGFLNRMPEILQNELPRVHVELGNWWSVTPGSQILWTTAVNNVLKGQRYK
EVNDDLKNLMLGRYGELPFYRPSDEIYCSVFGPEWKKIVEQEYGFQKVEDIDIEVEKKVLEHRLGREATEDELVLYLQHP
NDAVDFFKFEAKYGKTWVLPPKIWFKKGGFNLGEKFEILDAFGKLHIIEIGTQRKTKTGDAITYMLIDHHSQPILTELEG
EGPSAARKIQLTAKEIDALALSGDIRSHITGTVSEIPVSEGDEVSAGQILIILEAMKMLNNVVSEVNGQVSEIFVSPGDR
VEVGAPLLMIKKA
>Mature_653_residues
MKIKVYPMKISEHLLEERISPQDLKKKGVSFIVDKIRRSEGYYVTNTERDLSQSDFKNRVMPHTQLLVARERNEAGFFSI
EITGGASIHVDMLRKQINPLEKLEVLSANMPDTLFQTLCRGINMFGYRPYPENVIRLTVRSFARYVHIWRVFDFLNHVSN
MIPVFEEVRASGCLLEPAICFSTGPEHTDAYYVKKVGEILDVTGPDILLAIKNHGGLGTPKRIGELVRSILNAYPDLIIH
YHGHNTDGADVGRIVEAVRNGAKIVDAADHAFTGFYGPPPLLTVVDVLQEYGYRPSGLNRQAVMDTSNKLRPEREYYKDF
ESQFLGFDSTVQIHKLPGGATGSSFEQAVKGGFLNRMPEILQNELPRVHVELGNWWSVTPGSQILWTTAVNNVLKGQRYK
EVNDDLKNLMLGRYGELPFYRPSDEIYCSVFGPEWKKIVEQEYGFQKVEDIDIEVEKKVLEHRLGREATEDELVLYLQHP
NDAVDFFKFEAKYGKTWVLPPKIWFKKGGFNLGEKFEILDAFGKLHIIEIGTQRKTKTGDAITYMLIDHHSQPILTELEG
EGPSAARKIQLTAKEIDALALSGDIRSHITGTVSEIPVSEGDEVSAGQILIILEAMKMLNNVVSEVNGQVSEIFVSPGDR
VEVGAPLLMIKKA

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG1038

COG function: function code C; Pyruvate carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=641, Percent_Identity=25.7410296411856, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI106049295, Length=641, Percent_Identity=25.7410296411856, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI106049292, Length=641, Percent_Identity=25.7410296411856, Blast_Score=158, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17562816, Length=643, Percent_Identity=27.5272161741835, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321376, Length=651, Percent_Identity=27.0353302611367, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6319695, Length=652, Percent_Identity=27.3006134969325, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24652212, Length=641, Percent_Identity=26.8330733229329, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652210, Length=641, Percent_Identity=26.8330733229329, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652214, Length=641, Percent_Identity=26.8330733229329, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19921944, Length=641, Percent_Identity=26.8330733229329, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652216, Length=641, Percent_Identity=26.8330733229329, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI281363050, Length=641, Percent_Identity=26.8330733229329, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652224, Length=641, Percent_Identity=26.8330733229329, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652222, Length=641, Percent_Identity=26.8330733229329, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652220, Length=641, Percent_Identity=26.8330733229329, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652218, Length=641, Percent_Identity=26.8330733229329, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 73663; Mature: 73663

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKVYPMKISEHLLEERISPQDLKKKGVSFIVDKIRRSEGYYVTNTERDLSQSDFKNRV
CEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHCC
MPHTQLLVARERNEAGFFSIEITGGASIHVDMLRKQINPLEKLEVLSANMPDTLFQTLCR
CCCHHEEEEEECCCCCEEEEEECCCCEEHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHH
GINMFGYRPYPENVIRLTVRSFARYVHIWRVFDFLNHVSNMIPVFEEVRASGCLLEPAIC
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHH
FSTGPEHTDAYYVKKVGEILDVTGPDILLAIKNHGGLGTPKRIGELVRSILNAYPDLIIH
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
YHGHNTDGADVGRIVEAVRNGAKIVDAADHAFTGFYGPPPLLTVVDVLQEYGYRPSGLNR
ECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
QAVMDTSNKLRPEREYYKDFESQFLGFDSTVQIHKLPGGATGSSFEQAVKGGFLNRMPEI
HHEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHH
LQNELPRVHVELGNWWSVTPGSQILWTTAVNNVLKGQRYKEVNDDLKNLMLGRYGELPFY
HHCCCCEEEEEECCEEEECCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
RPSDEIYCSVFGPEWKKIVEQEYGFQKVEDIDIEVEKKVLEHRLGREATEDELVLYLQHP
CCCCCEEEEEECHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEECC
NDAVDFFKFEAKYGKTWVLPPKIWFKKGGFNLGEKFEILDAFGKLHIIEIGTQRKTKTGD
CCHHHHEEEECCCCCEEECCCHHHEECCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCC
AITYMLIDHHSQPILTELEGEGPSAARKIQLTAKEIDALALSGDIRSHITGTVSEIPVSE
EEEEEEEECCCCCCEEEECCCCCCCCEEEEEEHHHHHHHEECCCHHHHHCCCHHHCCCCC
GDEVSAGQILIILEAMKMLNNVVSEVNGQVSEIFVSPGDRVEVGAPLLMIKKA
CCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCEEEEECC
>Mature Secondary Structure
MKIKVYPMKISEHLLEERISPQDLKKKGVSFIVDKIRRSEGYYVTNTERDLSQSDFKNRV
CEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHCC
MPHTQLLVARERNEAGFFSIEITGGASIHVDMLRKQINPLEKLEVLSANMPDTLFQTLCR
CCCHHEEEEEECCCCCEEEEEECCCCEEHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHH
GINMFGYRPYPENVIRLTVRSFARYVHIWRVFDFLNHVSNMIPVFEEVRASGCLLEPAIC
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHH
FSTGPEHTDAYYVKKVGEILDVTGPDILLAIKNHGGLGTPKRIGELVRSILNAYPDLIIH
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
YHGHNTDGADVGRIVEAVRNGAKIVDAADHAFTGFYGPPPLLTVVDVLQEYGYRPSGLNR
ECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
QAVMDTSNKLRPEREYYKDFESQFLGFDSTVQIHKLPGGATGSSFEQAVKGGFLNRMPEI
HHEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHH
LQNELPRVHVELGNWWSVTPGSQILWTTAVNNVLKGQRYKEVNDDLKNLMLGRYGELPFY
HHCCCCEEEEEECCEEEECCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
RPSDEIYCSVFGPEWKKIVEQEYGFQKVEDIDIEVEKKVLEHRLGREATEDELVLYLQHP
CCCCCEEEEEECHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEECC
NDAVDFFKFEAKYGKTWVLPPKIWFKKGGFNLGEKFEILDAFGKLHIIEIGTQRKTKTGD
CCHHHHEEEECCCCCEEECCCHHHEECCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCC
AITYMLIDHHSQPILTELEGEGPSAARKIQLTAKEIDALALSGDIRSHITGTVSEIPVSE
EEEEEEEECCCCCCEEEECCCCCCCCEEEEEEHHHHHHHEECCCHHHHHCCCHHHCCCCC
GDEVSAGQILIILEAMKMLNNVVSEVNGQVSEIFVSPGDRVEVGAPLLMIKKA
CCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]