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The map label for this gene is pyc [H]

Identifier: 85859418

GI number: 85859418

Start: 1623723

End: 1625183

Strand: Direct

Name: pyc [H]

Synonym: SYN_01041

Alternate gene names: 85859418

Gene position: 1623723-1625183 (Clockwise)

Preceding gene: 85859417

Following gene: 85859419

Centisome position: 51.07

GC content: 50.92

Gene sequence:

>1461_bases
ATGGCTGTTACAAAGAAAAAGATAAAGAAGGTTCTGATTGCCAACAGAGGTGAGATTGCTCTGCGAATCCTCCGGACAGT
CAAGGAACTTTCCATGGATAGTGTCGTGATCTATGAAAAGCCGGACAGCGAAGCGTATTATATCCGTCTTGCCGACGACG
CTATCATGATTGGTGATGGACCACGGAAGGATTATCTGGATATCGATAAGATCATCTGGGCAGCCCGGAAAAGCGGTGCG
GATGCCATTCATCCCGGCTATGGGTTTCTGTCGGAAATTCCGGAATTTTCCGCGGAATGTGCGCGGGCCGGGATTGTTTT
TATTGGTCCGCCTCCCGATGTCATCCGCAATCTGGGTAATAAAGTCGTAGCCCGGGATATAATCGAGAAGGCGAACCTTC
CCTTTATTCCGGGAACAAAAGATTTATTCCGCGGTGATGCCGGACTTCGGGAAGCTATTGCCTTTGGACGCCGTTATGGT
TACCCCGTGATGCTGAAGGCTTCTTCAGGGGGTGGTGGCCGTGGGATCAGGAAGGTCATCAACGAGGCCGACCTCGAGAT
TCAGCTGCCCCAGGCGAGAGCGGAGGCACTTTCCGCGTTCGGCGACGAAAACGTCTATGTGGAAAAATGCATCGAAGCAC
CGCGGCATGTGGAAATACAGATCCTTGCCGACTCTTACGGTAATGTCATTCACCTGGGCTCGCGGGACTGTTCCATCCAG
AGACGTCATCAGAAACTCCTGGAAATCGCACCGGCAGATCTTCCCCCTGACGTGCTCAATGCCATGTACGGCGCCGCTAT
CGAAGCAGCCAGAGTCGCGGGATATGTCAATGCCGGAACGGTCGAGTTTCTTGTCGATTCCCGAACGAATGAATTCTGGT
TCATGGAGATGAATACCCGCCTTCAGGTTGAGCATACGGTGACGGAAGAATTGACCGGCGTGGATATCGTCCGGGAACAG
ATCCGGATTGCCGAAGGTGAGCATCTGAGTATTCCCCTGGAAAGAATTCATTTAATGGGTAAGGCTGTTCAGGTCCGGAT
TAATGCTGAAGATCCCAAGAACAATTTCATGCCGGAAGGGGGAAAAAGGGTGGAAGTTTATCAGTCCGCCGGCGGTCCGG
GAGTGCGACTTGATGGCCTGGTTTATCAGGGATATAAAATTCCCACGGAATACGATTCGCTGATGGTGAAAATGACCGTT
CGCGGATTCAACTGGGAGCAGACTATTCAAAGACTGAAAAGATCCCTCGAGGGGTTTCTCATTGTCGGGCTCAAAAGCAC
GGTGCCATTTTATCTTGCGATCTGTGACGAACCCGATTTCCAGGCGGGGAAATTCGATACCAGTTACATCGAAACGCACC
CTGAAATTTTCAATTACCCTGAAGCAGAGCGGGAAATTGCAAAACTTTCAAGGCTTATCGCCGAAACGCACGCAAAGAAG
ATCAATCCTTATGCCTATTAG

Upstream 100 bases:

>100_bases
CAATTTTGGAAAGTGATTTCAGAAATAACTCAACGTGCAGGTATTCCGGTAATTCCGCACAGATGAGATAGGTGAAGAGT
GTACGAGAGGAAGAGATAAT

Downstream 100 bases:

>100_bases
CCTGCGGCAAATCGTCCGGCCGCCCGCCGATGACGCTTCGCAGAAAATTCAAGCCATTCCCGGCATTTTCTCTCTGATGG
CGAGGAGGGGCTTTAACCTA

Product: pyruvate carboxylase biotin carboxylase subunit

Products: NA

Alternate protein names: Pyruvic carboxylase; PYC [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MAVTKKKIKKVLIANRGEIALRILRTVKELSMDSVVIYEKPDSEAYYIRLADDAIMIGDGPRKDYLDIDKIIWAARKSGA
DAIHPGYGFLSEIPEFSAECARAGIVFIGPPPDVIRNLGNKVVARDIIEKANLPFIPGTKDLFRGDAGLREAIAFGRRYG
YPVMLKASSGGGGRGIRKVINEADLEIQLPQARAEALSAFGDENVYVEKCIEAPRHVEIQILADSYGNVIHLGSRDCSIQ
RRHQKLLEIAPADLPPDVLNAMYGAAIEAARVAGYVNAGTVEFLVDSRTNEFWFMEMNTRLQVEHTVTEELTGVDIVREQ
IRIAEGEHLSIPLERIHLMGKAVQVRINAEDPKNNFMPEGGKRVEVYQSAGGPGVRLDGLVYQGYKIPTEYDSLMVKMTV
RGFNWEQTIQRLKRSLEGFLIVGLKSTVPFYLAICDEPDFQAGKFDTSYIETHPEIFNYPEAEREIAKLSRLIAETHAKK
INPYAY

Sequences:

>Translated_486_residues
MAVTKKKIKKVLIANRGEIALRILRTVKELSMDSVVIYEKPDSEAYYIRLADDAIMIGDGPRKDYLDIDKIIWAARKSGA
DAIHPGYGFLSEIPEFSAECARAGIVFIGPPPDVIRNLGNKVVARDIIEKANLPFIPGTKDLFRGDAGLREAIAFGRRYG
YPVMLKASSGGGGRGIRKVINEADLEIQLPQARAEALSAFGDENVYVEKCIEAPRHVEIQILADSYGNVIHLGSRDCSIQ
RRHQKLLEIAPADLPPDVLNAMYGAAIEAARVAGYVNAGTVEFLVDSRTNEFWFMEMNTRLQVEHTVTEELTGVDIVREQ
IRIAEGEHLSIPLERIHLMGKAVQVRINAEDPKNNFMPEGGKRVEVYQSAGGPGVRLDGLVYQGYKIPTEYDSLMVKMTV
RGFNWEQTIQRLKRSLEGFLIVGLKSTVPFYLAICDEPDFQAGKFDTSYIETHPEIFNYPEAEREIAKLSRLIAETHAKK
INPYAY
>Mature_485_residues
AVTKKKIKKVLIANRGEIALRILRTVKELSMDSVVIYEKPDSEAYYIRLADDAIMIGDGPRKDYLDIDKIIWAARKSGAD
AIHPGYGFLSEIPEFSAECARAGIVFIGPPPDVIRNLGNKVVARDIIEKANLPFIPGTKDLFRGDAGLREAIAFGRRYGY
PVMLKASSGGGGRGIRKVINEADLEIQLPQARAEALSAFGDENVYVEKCIEAPRHVEIQILADSYGNVIHLGSRDCSIQR
RHQKLLEIAPADLPPDVLNAMYGAAIEAARVAGYVNAGTVEFLVDSRTNEFWFMEMNTRLQVEHTVTEELTGVDIVREQI
RIAEGEHLSIPLERIHLMGKAVQVRINAEDPKNNFMPEGGKRVEVYQSAGGPGVRLDGLVYQGYKIPTEYDSLMVKMTVR
GFNWEQTIQRLKRSLEGFLIVGLKSTVPFYLAICDEPDFQAGKFDTSYIETHPEIFNYPEAEREIAKLSRLIAETHAKKI
NPYAY

Specific function: Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second, leading to oxaloacetate production. Fulfills an anaplerotic functi

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI116805327, Length=452, Percent_Identity=44.9115044247788, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI106049528, Length=471, Percent_Identity=45.6475583864119, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI106049295, Length=471, Percent_Identity=45.6475583864119, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI106049292, Length=471, Percent_Identity=45.6475583864119, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI189095269, Length=458, Percent_Identity=39.9563318777293, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI65506442, Length=458, Percent_Identity=39.7379912663755, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI295821183, Length=458, Percent_Identity=39.7379912663755, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI38679974, Length=516, Percent_Identity=30.8139534883721, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI38679971, Length=516, Percent_Identity=30.8139534883721, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI38679960, Length=516, Percent_Identity=30.8139534883721, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI38679977, Length=516, Percent_Identity=30.8139534883721, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI38679967, Length=516, Percent_Identity=30.8139534883721, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI134142062, Length=513, Percent_Identity=30.4093567251462, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI1789654, Length=446, Percent_Identity=46.8609865470852, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17562816, Length=480, Percent_Identity=45.8333333333333, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71987519, Length=467, Percent_Identity=43.254817987152, Blast_Score=349, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17567343, Length=457, Percent_Identity=40.9190371991247, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI133931226, Length=494, Percent_Identity=31.9838056680162, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71997168, Length=514, Percent_Identity=27.0428015564202, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71997163, Length=514, Percent_Identity=27.0428015564202, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319695, Length=476, Percent_Identity=44.9579831932773, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321376, Length=476, Percent_Identity=45.7983193277311, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6319685, Length=445, Percent_Identity=39.7752808988764, Blast_Score=320, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6323863, Length=477, Percent_Identity=32.0754716981132, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6324343, Length=529, Percent_Identity=29.6786389413989, Blast_Score=213, Evalue=4e-56,
Organism=Drosophila melanogaster, GI281363050, Length=480, Percent_Identity=43.5416666666667, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652224, Length=480, Percent_Identity=43.5416666666667, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652222, Length=480, Percent_Identity=43.5416666666667, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652220, Length=480, Percent_Identity=43.5416666666667, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652218, Length=480, Percent_Identity=43.5416666666667, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652212, Length=480, Percent_Identity=43.5416666666667, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652210, Length=480, Percent_Identity=43.5416666666667, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652214, Length=480, Percent_Identity=43.5416666666667, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI19921944, Length=480, Percent_Identity=43.5416666666667, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652216, Length=480, Percent_Identity=43.5416666666667, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24651757, Length=449, Percent_Identity=41.2026726057906, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24651759, Length=408, Percent_Identity=41.1764705882353, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI161076407, Length=507, Percent_Identity=30.7692307692308, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24586460, Length=507, Percent_Identity=30.7692307692308, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI161076409, Length=507, Percent_Identity=30.7692307692308, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24586458, Length=507, Percent_Identity=30.7692307692308, Blast_Score=207, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR000089
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR003379
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR000891
- InterPro:   IPR005930
- InterPro:   IPR011054
- InterPro:   IPR011053 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00364 Biotin_lipoyl; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 54270; Mature: 54139

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTKKKIKKVLIANRGEIALRILRTVKELSMDSVVIYEKPDSEAYYIRLADDAIMIGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCEEEECCC
PRKDYLDIDKIIWAARKSGADAIHPGYGFLSEIPEFSAECARAGIVFIGPPPDVIRNLGN
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCC
KVVARDIIEKANLPFIPGTKDLFRGDAGLREAIAFGRRYGYPVMLKASSGGGGRGIRKVI
HHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
NEADLEIQLPQARAEALSAFGDENVYVEKCIEAPRHVEIQILADSYGNVIHLGSRDCSIQ
CCCCCEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCHHH
RRHQKLLEIAPADLPPDVLNAMYGAAIEAARVAGYVNAGTVEFLVDSRTNEFWFMEMNTR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCE
LQVEHTVTEELTGVDIVREQIRIAEGEHLSIPLERIHLMGKAVQVRINAEDPKNNFMPEG
EEEEEHHHHHHCCHHHHHHHHHHCCCCEEECCHHHHHHCCEEEEEEEECCCCCCCCCCCC
GKRVEVYQSAGGPGVRLDGLVYQGYKIPTEYDSLMVKMTVRGFNWEQTIQRLKRSLEGFL
CCEEEEEECCCCCCEEECCEEEECEECCCCHHCEEEEEEECCCCHHHHHHHHHHHCCCEE
IVGLKSTVPFYLAICDEPDFQAGKFDTSYIETHPEIFNYPEAEREIAKLSRLIAETHAKK
EEEECCCCCEEEEEECCCCCCCCCCCCHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHC
INPYAY
CCCCCC
>Mature Secondary Structure 
AVTKKKIKKVLIANRGEIALRILRTVKELSMDSVVIYEKPDSEAYYIRLADDAIMIGDG
CCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCEEEECCC
PRKDYLDIDKIIWAARKSGADAIHPGYGFLSEIPEFSAECARAGIVFIGPPPDVIRNLGN
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCC
KVVARDIIEKANLPFIPGTKDLFRGDAGLREAIAFGRRYGYPVMLKASSGGGGRGIRKVI
HHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
NEADLEIQLPQARAEALSAFGDENVYVEKCIEAPRHVEIQILADSYGNVIHLGSRDCSIQ
CCCCCEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCHHH
RRHQKLLEIAPADLPPDVLNAMYGAAIEAARVAGYVNAGTVEFLVDSRTNEFWFMEMNTR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCE
LQVEHTVTEELTGVDIVREQIRIAEGEHLSIPLERIHLMGKAVQVRINAEDPKNNFMPEG
EEEEEHHHHHHCCHHHHHHHHHHCCCCEEECCHHHHHHCCEEEEEEEECCCCCCCCCCCC
GKRVEVYQSAGGPGVRLDGLVYQGYKIPTEYDSLMVKMTVRGFNWEQTIQRLKRSLEGFL
CCEEEEEECCCCCCEEECCEEEECEECCCCHHCEEEEEEECCCCHHHHHHHHHHHCCCEE
IVGLKSTVPFYLAICDEPDFQAGKFDTSYIETHPEIFNYPEAEREIAKLSRLIAETHAKK
EEEECCCCCEEEEEECCCCCCCCCCCCHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHC
INPYAY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]