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The map label for this gene is rluB [H]

Identifier: 85859158

GI number: 85859158

Start: 1333300

End: 1334055

Strand: Direct

Name: rluB [H]

Synonym: SYN_02856

Alternate gene names: 85859158

Gene position: 1333300-1334055 (Clockwise)

Preceding gene: 85859157

Following gene: 85859159

Centisome position: 41.94

GC content: 58.73

Gene sequence:

>756_bases
ATGGAAGAGCGGCTGCAGAAAATTCTCGCCCGATCGGGGGTGGCGTCCCGGCGTAAAGCGGAAGAGATGATCCGCGAAGG
GCGGGTTAGCGTCAATTCGGAAATCGTCCGGGAGCTGGGCTCAAAGGCCGACATCGAGCGGGACGAGATCCGTGTGGACG
GCAAGCTGATCTGCGCGGAAGAAACGAAGGTCTATGTAATGCTGAACAAGCCTCGAGGGTATGTGACGGCGCTCAGCGAT
CCGCAGAAACGGCCCCTGGTGATTCACCTCCTGCCGGAGTTGGGAGTCCGGCTTTTTCCCGTCGGCAGGCTGGATTACGA
TTCCGAAGGTCTGCTCCTGCTGACTAATGACGGCGGCTTCGCCCAGCGCGTCCAGCATCCCCGCTATCGTATTTCCAAGA
CCTATCGGGTCAAGATCTCCGGCAGACTGAATGCCGCGGAATTCAACCGCCTGCAGAAGGGGCTGGAGCTGGAAGACGGG
AAGTTTGCTCCCGATGAGGTGGCGCTCGAAGAGATCAATCCAAAGAGCAGCTGGTTGACCATGAAGATCAGTGAAGGGCG
CAACCGGGTTATCCGCCGGGCCTTCGAGCAACTGGGGCACGAGGTCCTGAGGCTGATCCGGACGGCTGTTGCGGATCTGC
ATCTGGGCAATCTTCGGGAAGGAGATTACCGGCTTCTCAAGGGCTGGGAGGTTCAGCAGCTTCTGACGCCGCGAAAGAGG
AGCGGATCGAAGATTCTGCCGGAAAAGGAAAAATAA

Upstream 100 bases:

>100_bases
CAGGCGGACGCAAGGCGATCTTGTGGAACCGGCGTCGGGCAGAAAGCGAAGAACGCGCTTGAATCTGGAATCGGAATGAA
AGGAAGGGCAGGACATCATC

Downstream 100 bases:

>100_bases
GAATGAGGACAATTCTCTGCTTCAGGATATGGCCGGCGGCAATGGTTCTGATCTGTCTGATTGTCCTGTGCGGCAGGGCG
GAGGCGACGCCGGTGGCAGC

Product: RNA pseudouridylate synthase S4 domain-containing protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MEERLQKILARSGVASRRKAEEMIREGRVSVNSEIVRELGSKADIERDEIRVDGKLICAEETKVYVMLNKPRGYVTALSD
PQKRPLVIHLLPELGVRLFPVGRLDYDSEGLLLLTNDGGFAQRVQHPRYRISKTYRVKISGRLNAAEFNRLQKGLELEDG
KFAPDEVALEEINPKSSWLTMKISEGRNRVIRRAFEQLGHEVLRLIRTAVADLHLGNLREGDYRLLKGWEVQQLLTPRKR
SGSKILPEKEK

Sequences:

>Translated_251_residues
MEERLQKILARSGVASRRKAEEMIREGRVSVNSEIVRELGSKADIERDEIRVDGKLICAEETKVYVMLNKPRGYVTALSD
PQKRPLVIHLLPELGVRLFPVGRLDYDSEGLLLLTNDGGFAQRVQHPRYRISKTYRVKISGRLNAAEFNRLQKGLELEDG
KFAPDEVALEEINPKSSWLTMKISEGRNRVIRRAFEQLGHEVLRLIRTAVADLHLGNLREGDYRLLKGWEVQQLLTPRKR
SGSKILPEKEK
>Mature_251_residues
MEERLQKILARSGVASRRKAEEMIREGRVSVNSEIVRELGSKADIERDEIRVDGKLICAEETKVYVMLNKPRGYVTALSD
PQKRPLVIHLLPELGVRLFPVGRLDYDSEGLLLLTNDGGFAQRVQHPRYRISKTYRVKISGRLNAAEFNRLQKGLELEDG
KFAPDEVALEEINPKSSWLTMKISEGRNRVIRRAFEQLGHEVLRLIRTAVADLHLGNLREGDYRLLKGWEVQQLLTPRKR
SGSKILPEKEK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=221, Percent_Identity=39.3665158371041, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=35.4700854700855, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI87081838, Length=174, Percent_Identity=36.7816091954023, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790453, Length=249, Percent_Identity=29.3172690763052, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28760; Mature: 28760

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERLQKILARSGVASRRKAEEMIREGRVSVNSEIVRELGSKADIERDEIRVDGKLICAE
CHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHEEECCEEEEEC
ETKVYVMLNKPRGYVTALSDPQKRPLVIHLLPELGVRLFPVGRLDYDSEGLLLLTNDGGF
CCEEEEEEECCCCEEEECCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCEEEEECCCCH
AQRVQHPRYRISKTYRVKISGRLNAAEFNRLQKGLELEDGKFAPDEVALEEINPKSSWLT
HHHHCCCCCEECEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEE
MKISEGRNRVIRRAFEQLGHEVLRLIRTAVADLHLGNLREGDYRLLKGWEVQQLLTPRKR
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHCCCCC
SGSKILPEKEK
CCCCCCCCCCC
>Mature Secondary Structure
MEERLQKILARSGVASRRKAEEMIREGRVSVNSEIVRELGSKADIERDEIRVDGKLICAE
CHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHEEECCEEEEEC
ETKVYVMLNKPRGYVTALSDPQKRPLVIHLLPELGVRLFPVGRLDYDSEGLLLLTNDGGF
CCEEEEEEECCCCEEEECCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCEEEEECCCCH
AQRVQHPRYRISKTYRVKISGRLNAAEFNRLQKGLELEDGKFAPDEVALEEINPKSSWLT
HHHHCCCCCEECEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEE
MKISEGRNRVIRRAFEQLGHEVLRLIRTAVADLHLGNLREGDYRLLKGWEVQQLLTPRKR
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHCCCCC
SGSKILPEKEK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]