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The map label for this gene is thiDE [H]

Identifier: 85859111

GI number: 85859111

Start: 1288104

End: 1288985

Strand: Direct

Name: thiDE [H]

Synonym: SYN_01521

Alternate gene names: 85859111

Gene position: 1288104-1288985 (Clockwise)

Preceding gene: 85859110

Following gene: 85859112

Centisome position: 40.52

GC content: 62.13

Gene sequence:

>882_bases
ATGAACAGAACACCGGCGCGCGTCCTGACGATTGCCGGATCGGATTCCGGAGGCGGAGCGGGGATTCAGGCCGATCTGAA
AACGATCACCGTGCTGGGCGGTTTCGGGATGAGTGTGATCACCGCCCTGACGGCCCAGAATACCCTGGGGGTCCAGGGCA
TCTTCGAGGTGCCGATTTCCTTCATCGAACAGCAGTTCGATGCCGTAGCCACCGATATCGGTGTGGACGCTGCCAAGACG
GGCATGCTGGTCAGTGCCGCCGTTGTGCGCGCCGTGGCGGCAAAAATCCGGCAGCACCGCATCGAAAAGCTGGTGGTCGA
TCCGGTTCTGACGGCCAAGGACGGTGTCCGGCTGATCGAAGATGAAGCGATGGAAACCCTGGTTTCGGACCTGCTCCCTC
TGGCCTTCGTCGTGACGCCCAATGTGCCGGAGGCAGAAGTCATGGCGGAGATGACCATCTCATCCGTCGCCGATATGAAG
GAAGCGGCGCGCAGGATCCGACGGTTCGGCGTTCCCAACGTAATCGTCAAGGGCGGTCATCTGCCTGGGGAGGCGCTGGA
TATTCTTTACGACGGCAGCACATATCACGAATTTGCGGCCGAGCGGATCGAGACGAAGGACACCCACGGGACGGGATGCA
CCTTTTCGGCTGCGCTTGCCACCGGCCTCGCCCTGGGAAAGCCTCTCCACGACTCGGTCCGGGAGGCGAAGTCCTACATT
ACGGAAGTCATCCGCTACGCCTGGCGAATCGGCGGGGGGCACGGCCCGACGAATCATGCGGCCCCGCTTTTCAACGCCCT
TGACCGCTGCAGGCGTCATTCCGATGGCGGATCAAATAAGAACCCGGAGCGGCGGGACGAGAGCGACTCCGGCATTCGGT
GA

Upstream 100 bases:

>100_bases
TCCTCACGGGAAAACAGGGCGTCATCTCCCTTTCCGACGGCATTCTGCTGGTCATTCATTACTATCGCAAGGGACATTTT
CCCCAGGGGGCCTGATCCCG

Downstream 100 bases:

>100_bases
CGGCCCCGGGGCCGTCAGGAAGCTGACGGCAAAGACGCTTTGATATGAAACAGCAATGATATATAAGATTCTGCGATGCA
ATAACCAGGGAGGATGGTAG

Product: phosphomethylpyrimidine kinase / hydroxymethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNRTPARVLTIAGSDSGGGAGIQADLKTITVLGGFGMSVITALTAQNTLGVQGIFEVPISFIEQQFDAVATDIGVDAAKT
GMLVSAAVVRAVAAKIRQHRIEKLVVDPVLTAKDGVRLIEDEAMETLVSDLLPLAFVVTPNVPEAEVMAEMTISSVADMK
EAARRIRRFGVPNVIVKGGHLPGEALDILYDGSTYHEFAAERIETKDTHGTGCTFSAALATGLALGKPLHDSVREAKSYI
TEVIRYAWRIGGGHGPTNHAAPLFNALDRCRRHSDGGSNKNPERRDESDSGIR

Sequences:

>Translated_293_residues
MNRTPARVLTIAGSDSGGGAGIQADLKTITVLGGFGMSVITALTAQNTLGVQGIFEVPISFIEQQFDAVATDIGVDAAKT
GMLVSAAVVRAVAAKIRQHRIEKLVVDPVLTAKDGVRLIEDEAMETLVSDLLPLAFVVTPNVPEAEVMAEMTISSVADMK
EAARRIRRFGVPNVIVKGGHLPGEALDILYDGSTYHEFAAERIETKDTHGTGCTFSAALATGLALGKPLHDSVREAKSYI
TEVIRYAWRIGGGHGPTNHAAPLFNALDRCRRHSDGGSNKNPERRDESDSGIR
>Mature_293_residues
MNRTPARVLTIAGSDSGGGAGIQADLKTITVLGGFGMSVITALTAQNTLGVQGIFEVPISFIEQQFDAVATDIGVDAAKT
GMLVSAAVVRAVAAKIRQHRIEKLVVDPVLTAKDGVRLIEDEAMETLVSDLLPLAFVVTPNVPEAEVMAEMTISSVADMK
EAARRIRRFGVPNVIVKGGHLPGEALDILYDGSTYHEFAAERIETKDTHGTGCTFSAALATGLALGKPLHDSVREAKSYI
TEVIRYAWRIGGGHGPTNHAAPLFNALDRCRRHSDGGSNKNPERRDESDSGIR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=259, Percent_Identity=44.4015444015444, Blast_Score=198, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6325378, Length=300, Percent_Identity=32.6666666666667, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324997, Length=301, Percent_Identity=35.2159468438538, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324517, Length=296, Percent_Identity=33.445945945946, Blast_Score=119, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 31169; Mature: 31169

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTPARVLTIAGSDSGGGAGIQADLKTITVLGGFGMSVITALTAQNTLGVQGIFEVPIS
CCCCCCEEEEEECCCCCCCCCEEEHHHEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHH
FIEQQFDAVATDIGVDAAKTGMLVSAAVVRAVAAKIRQHRIEKLVVDPVLTAKDGVRLIE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHH
DEAMETLVSDLLPLAFVVTPNVPEAEVMAEMTISSVADMKEAARRIRRFGVPNVIVKGGH
HHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
LPGEALDILYDGSTYHEFAAERIETKDTHGTGCTFSAALATGLALGKPLHDSVREAKSYI
CCCHHEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
TEVIRYAWRIGGGHGPTNHAAPLFNALDRCRRHSDGGSNKNPERRDESDSGIR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRTPARVLTIAGSDSGGGAGIQADLKTITVLGGFGMSVITALTAQNTLGVQGIFEVPIS
CCCCCCEEEEEECCCCCCCCCEEEHHHEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHH
FIEQQFDAVATDIGVDAAKTGMLVSAAVVRAVAAKIRQHRIEKLVVDPVLTAKDGVRLIE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHH
DEAMETLVSDLLPLAFVVTPNVPEAEVMAEMTISSVADMKEAARRIRRFGVPNVIVKGGH
HHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
LPGEALDILYDGSTYHEFAAERIETKDTHGTGCTFSAALATGLALGKPLHDSVREAKSYI
CCCHHEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
TEVIRYAWRIGGGHGPTNHAAPLFNALDRCRRHSDGGSNKNPERRDESDSGIR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA