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The map label for this gene is thiC

Identifier: 85859112

GI number: 85859112

Start: 1289093

End: 1290376

Strand: Direct

Name: thiC

Synonym: SYN_01520

Alternate gene names: 85859112

Gene position: 1289093-1290376 (Clockwise)

Preceding gene: 85859111

Following gene: 85859113

Centisome position: 40.55

GC content: 62.15

Gene sequence:

>1284_bases
ATGACGCAGCTGGAGATGGCCGGACAGGGCCGTGTTACCGAGGAAATGAAATTATGCGCCGAGAAGGAAGGCGTGTCGCC
GGAATTTATCCGCCGGGGGGTGGAAGAGGGCAGTATCGTGGTCGTCCGCAACAACCGCCACACAGGCATTGCACCCCTCG
CCATAGGAAAGGGGCTGCGGACCAAGGTCAACGCCAATCTCGGGACATCCCGGGATCATGTGGATCTGGAAATGGAACTG
GAAAAAGTGCGGATCTGCACAAAAGCGGGCGCCGATGCCCTGATGGATCTGTCCACGGGAGGCGAAATCCGGGCGATCCG
GCAGGCCATTGTGAACGCTTCCACCATGGCGGTAGGCACCGTCCCCATTTATGCCGCCGCCGCCGAGGCCCTGAACAGCC
GGAAATCCATCATGGAAATGACGGCGGATGAAATGTTCCAGGCCATCGAGGAAAACGGCGAGGACGGCGTGGATTTCATC
ACCGTCCACTGCGGAGTGACCCGCGAGAGCGTGGGCCGCATCGCCGCCCAGGGACGCCTCCTGGGGATCGTCAGCCGGGG
CGGCTCCATTACGGCCCGATGGATGGATTACAATGATGCGGAAAATCCCCTCTACGCGCAGTATGACCGACTCCTGGAAA
TCGCCCGGCGCTACGACATGGTCCTCAGCCTCGGTGACGGCCTGCGGCCCGGGTGTCTTGCCGACGCGACGGACCGGGGC
CAGGTTCAGGAACTCATTATTCTCGGTGAACTGTGCCGGCGGGCGCGCGCGCGGGATGTCCAGGTCATGATCGAAGGTCC
GGGTCACGTTCCCTATCCCCAGATCGAGGCCAACATCGCCCTGCAGAAGAGCCTCTGCGAAGGCGCGCCCTTTTATGTCC
TGGGGCCGCTCCCCACGGACATCGCCCCCGGCTATGACCACATCACGGCGGCAATCGGCGGCGCGCTGGCTGGAGCCGCC
GGAGCGGACTTTCTCTGCTATGTGACGCCGTCGGAACATCTTTGTCTGCCCAACCTCGATGACGTGCATGAGGGGATTGT
GGCCTCCCGGATCGCCGCCCACATTGCGGACATCGCCAAGGGATATCCCGGGGCGATGGAAAAGGATATCCTGATGGCGA
AATACCGCAGTGAATTCAACTGGAATGGGCAGATCGACCTGTCCGTCGATCCGGACCGGAGCCGGGCACGGCTGGAGCGC
AGCGAAACGGCCAAAGAGGAAGGCTGTACCATGTGCGGCGATCTGTGCGCCATCAAGCTCGGGAAAAAGAAAGCCCCCTG
TTAG

Upstream 100 bases:

>100_bases
GGGGCCGTCAGGAAGCTGACGGCAAAGACGCTTTGATATGAAACAGCAATGATATATAAGATTCTGCGATGCAATAACCA
GGGAGGATGGTAGAAACGAA

Downstream 100 bases:

>100_bases
ACGGATGGCCTGCCCCATGACGATCCGCGATCCGGGAGAACGTACCGAATTGGTCTCGCGCCGGGATATCGCCGCCGGGA
TCGTCCGCCGTTTGAAGGAA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTQLEMAGQGRVTEEMKLCAEKEGVSPEFIRRGVEEGSIVVVRNNRHTGIAPLAIGKGLRTKVNANLGTSRDHVDLEMEL
EKVRICTKAGADALMDLSTGGEIRAIRQAIVNASTMAVGTVPIYAAAAEALNSRKSIMEMTADEMFQAIEENGEDGVDFI
TVHCGVTRESVGRIAAQGRLLGIVSRGGSITARWMDYNDAENPLYAQYDRLLEIARRYDMVLSLGDGLRPGCLADATDRG
QVQELIILGELCRRARARDVQVMIEGPGHVPYPQIEANIALQKSLCEGAPFYVLGPLPTDIAPGYDHITAAIGGALAGAA
GADFLCYVTPSEHLCLPNLDDVHEGIVASRIAAHIADIAKGYPGAMEKDILMAKYRSEFNWNGQIDLSVDPDRSRARLER
SETAKEEGCTMCGDLCAIKLGKKKAPC

Sequences:

>Translated_427_residues
MTQLEMAGQGRVTEEMKLCAEKEGVSPEFIRRGVEEGSIVVVRNNRHTGIAPLAIGKGLRTKVNANLGTSRDHVDLEMEL
EKVRICTKAGADALMDLSTGGEIRAIRQAIVNASTMAVGTVPIYAAAAEALNSRKSIMEMTADEMFQAIEENGEDGVDFI
TVHCGVTRESVGRIAAQGRLLGIVSRGGSITARWMDYNDAENPLYAQYDRLLEIARRYDMVLSLGDGLRPGCLADATDRG
QVQELIILGELCRRARARDVQVMIEGPGHVPYPQIEANIALQKSLCEGAPFYVLGPLPTDIAPGYDHITAAIGGALAGAA
GADFLCYVTPSEHLCLPNLDDVHEGIVASRIAAHIADIAKGYPGAMEKDILMAKYRSEFNWNGQIDLSVDPDRSRARLER
SETAKEEGCTMCGDLCAIKLGKKKAPC
>Mature_426_residues
TQLEMAGQGRVTEEMKLCAEKEGVSPEFIRRGVEEGSIVVVRNNRHTGIAPLAIGKGLRTKVNANLGTSRDHVDLEMELE
KVRICTKAGADALMDLSTGGEIRAIRQAIVNASTMAVGTVPIYAAAAEALNSRKSIMEMTADEMFQAIEENGEDGVDFIT
VHCGVTRESVGRIAAQGRLLGIVSRGGSITARWMDYNDAENPLYAQYDRLLEIARRYDMVLSLGDGLRPGCLADATDRGQ
VQELIILGELCRRARARDVQVMIEGPGHVPYPQIEANIALQKSLCEGAPFYVLGPLPTDIAPGYDHITAAIGGALAGAAG
ADFLCYVTPSEHLCLPNLDDVHEGIVASRIAAHIADIAKGYPGAMEKDILMAKYRSEFNWNGQIDLSVDPDRSRARLERS
ETAKEEGCTMCGDLCAIKLGKKKAPC

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=458, Percent_Identity=43.4497816593886, Blast_Score=348, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_SYNAS (Q2LSU0)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461314.1
- STRING:   Q2LSU0
- GeneID:   3883192
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_01520
- NMPDR:   fig|56780.10.peg.1252
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- PhylomeDB:   Q2LSU0
- ProtClustDB:   PRK13352
- BioCyc:   SACI56780:SYN_01520-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 46086; Mature: 45955

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 95-95 BINDING 124-124 BINDING 163-163 BINDING 265-265 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLEMAGQGRVTEEMKLCAEKEGVSPEFIRRGVEEGSIVVVRNNRHTGIAPLAIGKGLR
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCCCC
TKVNANLGTSRDHVDLEMELEKVRICTKAGADALMDLSTGGEIRAIRQAIVNASTMAVGT
EEECCCCCCCCCCEEEEEEHHHHHHHHHCCCHHEEECCCCCHHHHHHHHHHCCHHEEEEC
VPIYAAAAEALNSRKSIMEMTADEMFQAIEENGEDGVDFITVHCGVTRESVGRIAAQGRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCEE
LGIVSRGGSITARWMDYNDAENPLYAQYDRLLEIARRYDMVLSLGDGLRPGCLADATDRG
EEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
QVQELIILGELCRRARARDVQVMIEGPGHVPYPQIEANIALQKSLCEGAPFYVLGPLPTD
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCC
IAPGYDHITAAIGGALAGAAGADFLCYVTPSEHLCLPNLDDVHEGIVASRIAAHIADIAK
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GYPGAMEKDILMAKYRSEFNWNGQIDLSVDPDRSRARLERSETAKEEGCTMCGDLCAIKL
CCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHEEE
GKKKAPC
CCCCCCC
>Mature Secondary Structure 
TQLEMAGQGRVTEEMKLCAEKEGVSPEFIRRGVEEGSIVVVRNNRHTGIAPLAIGKGLR
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCCCC
TKVNANLGTSRDHVDLEMELEKVRICTKAGADALMDLSTGGEIRAIRQAIVNASTMAVGT
EEECCCCCCCCCCEEEEEEHHHHHHHHHCCCHHEEECCCCCHHHHHHHHHHCCHHEEEEC
VPIYAAAAEALNSRKSIMEMTADEMFQAIEENGEDGVDFITVHCGVTRESVGRIAAQGRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCEE
LGIVSRGGSITARWMDYNDAENPLYAQYDRLLEIARRYDMVLSLGDGLRPGCLADATDRG
EEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
QVQELIILGELCRRARARDVQVMIEGPGHVPYPQIEANIALQKSLCEGAPFYVLGPLPTD
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCC
IAPGYDHITAAIGGALAGAAGADFLCYVTPSEHLCLPNLDDVHEGIVASRIAAHIADIAK
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GYPGAMEKDILMAKYRSEFNWNGQIDLSVDPDRSRARLERSETAKEEGCTMCGDLCAIKL
CCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHEEE
GKKKAPC
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA