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The map label for this gene is thiN [H]

Identifier: 85859110

GI number: 85859110

Start: 1287391

End: 1288098

Strand: Direct

Name: thiN [H]

Synonym: SYN_01522

Alternate gene names: 85859110

Gene position: 1287391-1288098 (Clockwise)

Preceding gene: 85859109

Following gene: 85859111

Centisome position: 40.49

GC content: 59.32

Gene sequence:

>708_bases
ATGGGTATGGCCCTTTCCTGTCCGGAGGAGGGCGTCATGAAAGAAAAAGCCGTTTTAGTCTGCGGCGGAGAGATCCGGGA
TTATTCCTGGCTGCGGACGAGGATCGCCGCGCTGGATCCCTGCAGGATCATCTGTGCCGACGTCGGCGCCTGTCACTGCC
TGAACATCGATGTCTCCCCGGAAGTGGTTATCGGCGATTTCGATTCCCTGAATCCGTCGCTGGCGGAATCCCTTGCCCGG
CGCGGGACGCGAATCTCAGGCTACCCCCCGGAAAAGGATCAGACGGACACCCAGCTTGCGCTGGAGTATGCCCTTTCCCT
GGAGCCGGAGGAAATACGGATCTTCGGAGCTCTGGGTGGACGGATCGATCATACCCTGGCCAACATGTCCATCCTGAAGA
TCGCCCTCGATCAGGGCGTGCCGGCCCGGCTTATCGATGAATGGAGCGAGATCCTGCTCACCCGCGACCGCTGCGTCCTG
GAAGGCGAGGAAGGCCAGACCGTTTCCCTGTTTCCCTTCACCACATCCGTGACGGGCCTGACGCTTCAGGGATTTGCCTA
TCCCCTGGAAAACGACGTTATGGAAGTCGGGCGGCCTTATGGGATCAGCAATGTCCTCACGGGAAAACAGGGCGTCATCT
CCCTTTCCGACGGCATTCTGCTGGTCATTCATTACTATCGCAAGGGACATTTTCCCCAGGGGGCCTGA

Upstream 100 bases:

>100_bases
TGAGGAACTCGCGGAAATAACATCGCGAAATGCCAGTGAAATATTTTCGATTAAACTATAAGTCAGGACGGGAAAACGGA
GGAAAGGGCCATATCCCGGA

Downstream 100 bases:

>100_bases
TCCCGATGAACAGAACACCGGCGCGCGTCCTGACGATTGCCGGATCGGATTCCGGAGGCGGAGCGGGGATTCAGGCCGAT
CTGAAAACGATCACCGTGCT

Product: thiamin pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MGMALSCPEEGVMKEKAVLVCGGEIRDYSWLRTRIAALDPCRIICADVGACHCLNIDVSPEVVIGDFDSLNPSLAESLAR
RGTRISGYPPEKDQTDTQLALEYALSLEPEEIRIFGALGGRIDHTLANMSILKIALDQGVPARLIDEWSEILLTRDRCVL
EGEEGQTVSLFPFTTSVTGLTLQGFAYPLENDVMEVGRPYGISNVLTGKQGVISLSDGILLVIHYYRKGHFPQGA

Sequences:

>Translated_235_residues
MGMALSCPEEGVMKEKAVLVCGGEIRDYSWLRTRIAALDPCRIICADVGACHCLNIDVSPEVVIGDFDSLNPSLAESLAR
RGTRISGYPPEKDQTDTQLALEYALSLEPEEIRIFGALGGRIDHTLANMSILKIALDQGVPARLIDEWSEILLTRDRCVL
EGEEGQTVSLFPFTTSVTGLTLQGFAYPLENDVMEVGRPYGISNVLTGKQGVISLSDGILLVIHYYRKGHFPQGA
>Mature_234_residues
GMALSCPEEGVMKEKAVLVCGGEIRDYSWLRTRIAALDPCRIICADVGACHCLNIDVSPEVVIGDFDSLNPSLAESLARR
GTRISGYPPEKDQTDTQLALEYALSLEPEEIRIFGALGGRIDHTLANMSILKIALDQGVPARLIDEWSEILLTRDRCVLE
GEEGQTVSLFPFTTSVTGLTLQGFAYPLENDVMEVGRPYGISNVLTGKQGVISLSDGILLVIHYYRKGHFPQGA

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 25627; Mature: 25496

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMALSCPEEGVMKEKAVLVCGGEIRDYSWLRTRIAALDPCRIICADVGACHCLNIDVSP
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHEEEECCCCEEEEECCCCC
EVVIGDFDSLNPSLAESLARRGTRISGYPPEKDQTDTQLALEYALSLEPEEIRIFGALGG
CEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCC
RIDHTLANMSILKIALDQGVPARLIDEWSEILLTRDRCVLEGEEGQTVSLFPFTTSVTGL
CHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCE
TLQGFAYPLENDVMEVGRPYGISNVLTGKQGVISLSDGILLVIHYYRKGHFPQGA
EEECEECCCHHHHHHCCCCCCCCHHCCCCCCEEEECCCEEEEEEEHHCCCCCCCC
>Mature Secondary Structure 
GMALSCPEEGVMKEKAVLVCGGEIRDYSWLRTRIAALDPCRIICADVGACHCLNIDVSP
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHEEEECCCCEEEEECCCCC
EVVIGDFDSLNPSLAESLARRGTRISGYPPEKDQTDTQLALEYALSLEPEEIRIFGALGG
CEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCC
RIDHTLANMSILKIALDQGVPARLIDEWSEILLTRDRCVLEGEEGQTVSLFPFTTSVTGL
CHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCE
TLQGFAYPLENDVMEVGRPYGISNVLTGKQGVISLSDGILLVIHYYRKGHFPQGA
EEECEECCCHHHHHHCCCCCCCCHHCCCCCCEEEECCCEEEEEEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]