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The map label for this gene is metK [H]

Identifier: 85859083

GI number: 85859083

Start: 1257789

End: 1258967

Strand: Direct

Name: metK [H]

Synonym: SYN_00417

Alternate gene names: 85859083

Gene position: 1257789-1258967 (Clockwise)

Preceding gene: 85859082

Following gene: 85859084

Centisome position: 39.56

GC content: 50.04

Gene sequence:

>1179_bases
ATGCAGATTACGTCAGAAAGTGTCAAAGTGGGTCATCCGGATATCGTTGCGGATTCCATCGCTGCCAACATCATCGCTGC
CATTCTGGATGAAGAAAAGAAGATCGGATTGACCGTGGACAACATGCCGCATTGCGGTATCGAGGTCTTTCTGGGCAAGG
GACTCTGTATTGTCGGAGGCGAAGTCGCCACCAGGATTTATGTGGACATCGAACGATGCGTCCGGGATACGGTGTTGAAA
CTCGGTTATTGTGATTATGAACTCGGTCTGAATGGAAATTCCATGGGAATACTCAATACGATTATTCCTCAATCACCCGA
CATCAACATCGGAACCCGGGCTGATCTGGGAAAGTACAAGGAGATCGGCGCGGGAGATCAAGGCATTATTTACGGATTCG
CCTGTGATGAAACTCCGGAACTGCTGCCTCTGCCTTATGTATTGGCCACTAAGATGATGCGTGTTTTTGAAGAAGCCAAA
AATCCGGTCTTCGCTCCTGATGGAAAAGGGCAGATCACGGTAGAGTATAATGACGAAGGGAAACCGGTTCGGGTCGCAAC
GGTGCTGATGTCCAATGCCATTGATTATCGCCATGTGCCGGAGGGATTCCGGAACAGCATAGAACCTCTGGCCAAGCAGA
TGGCCATGAATTGTTTCCGTGAATGGGTGGATGAGCAGACCGATTTTCTCTTTAATCCGACAGGCGAATGGCAGTCCGTT
AATTCCTGCAGCGCGGCGGATTCCGGTGTAACAGGAAGAAAACTCGTTGTTCAGCTTTATGGCGGTTATCCCGGAGCCCA
ACTGGGCGGAGGGTCTATTGTCAACAAGACGCCGGAAAAGGTCGATTGTTCCGCGGCACTTGGAGCCCGTTACGTAGCAA
AGAATATCGTTGCCGCGGGATTAGCCGGCAAGTGTTCCATTCAACTGTCCTATGCCATCGGTATCGCACGTCCGATCTCA
ATTTATGTGCATACCTTCGGGACAGGGGTCATATCCGATAACAAGATAGGAACGATCGTCAGAGAATACTTTGATCTGTC
ACCGCGGGGCATGATTGAGCGATTCAATCTCCTGGATGGGGAGAAGTACAGGAAACTGCCGAAGACGCTTTTCCTTGATG
ACTACCCCTGGGAAAAAACGGACATGGTGGATTCACTCAAAAAGGCCGCTGACATATAA

Upstream 100 bases:

>100_bases
CTTGGGAGTGATGGGTGCTTCCCACGTTTTCATGGTGTTGCTTTGTAGATAACTTACATGAAATTATTAAAGAAAGAGAC
AGGATAAAGGAGAAAGGTAA

Downstream 100 bases:

>100_bases
GCGCGGCCAGGCCAATGCGAAGGAGAAATACATGGAATTTCTGGAGATTGACGGTTTACTGCCCTATCGGGTAGCCGACC
TTTCCCTGGCGGAATGGGGA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MQITSESVKVGHPDIVADSIAANIIAAILDEEKKIGLTVDNMPHCGIEVFLGKGLCIVGGEVATRIYVDIERCVRDTVLK
LGYCDYELGLNGNSMGILNTIIPQSPDINIGTRADLGKYKEIGAGDQGIIYGFACDETPELLPLPYVLATKMMRVFEEAK
NPVFAPDGKGQITVEYNDEGKPVRVATVLMSNAIDYRHVPEGFRNSIEPLAKQMAMNCFREWVDEQTDFLFNPTGEWQSV
NSCSAADSGVTGRKLVVQLYGGYPGAQLGGGSIVNKTPEKVDCSAALGARYVAKNIVAAGLAGKCSIQLSYAIGIARPIS
IYVHTFGTGVISDNKIGTIVREYFDLSPRGMIERFNLLDGEKYRKLPKTLFLDDYPWEKTDMVDSLKKAADI

Sequences:

>Translated_392_residues
MQITSESVKVGHPDIVADSIAANIIAAILDEEKKIGLTVDNMPHCGIEVFLGKGLCIVGGEVATRIYVDIERCVRDTVLK
LGYCDYELGLNGNSMGILNTIIPQSPDINIGTRADLGKYKEIGAGDQGIIYGFACDETPELLPLPYVLATKMMRVFEEAK
NPVFAPDGKGQITVEYNDEGKPVRVATVLMSNAIDYRHVPEGFRNSIEPLAKQMAMNCFREWVDEQTDFLFNPTGEWQSV
NSCSAADSGVTGRKLVVQLYGGYPGAQLGGGSIVNKTPEKVDCSAALGARYVAKNIVAAGLAGKCSIQLSYAIGIARPIS
IYVHTFGTGVISDNKIGTIVREYFDLSPRGMIERFNLLDGEKYRKLPKTLFLDDYPWEKTDMVDSLKKAADI
>Mature_392_residues
MQITSESVKVGHPDIVADSIAANIIAAILDEEKKIGLTVDNMPHCGIEVFLGKGLCIVGGEVATRIYVDIERCVRDTVLK
LGYCDYELGLNGNSMGILNTIIPQSPDINIGTRADLGKYKEIGAGDQGIIYGFACDETPELLPLPYVLATKMMRVFEEAK
NPVFAPDGKGQITVEYNDEGKPVRVATVLMSNAIDYRHVPEGFRNSIEPLAKQMAMNCFREWVDEQTDFLFNPTGEWQSV
NSCSAADSGVTGRKLVVQLYGGYPGAQLGGGSIVNKTPEKVDCSAALGARYVAKNIVAAGLAGKCSIQLSYAIGIARPIS
IYVHTFGTGVISDNKIGTIVREYFDLSPRGMIERFNLLDGEKYRKLPKTLFLDDYPWEKTDMVDSLKKAADI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=384, Percent_Identity=34.375, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI4557737, Length=385, Percent_Identity=34.2857142857143, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1789311, Length=395, Percent_Identity=41.2658227848101, Blast_Score=271, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI17538498, Length=405, Percent_Identity=34.5679012345679, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=34.6938775510204, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=35.2185089974293, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17538494, Length=393, Percent_Identity=34.3511450381679, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI32565911, Length=351, Percent_Identity=35.042735042735, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI25145635, Length=338, Percent_Identity=35.207100591716, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI32565903, Length=331, Percent_Identity=35.3474320241692, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI32565905, Length=391, Percent_Identity=31.4578005115089, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6323209, Length=395, Percent_Identity=37.4683544303797, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6320710, Length=367, Percent_Identity=37.3297002724796, Blast_Score=209, Evalue=7e-55,
Organism=Drosophila melanogaster, GI45552159, Length=362, Percent_Identity=35.9116022099448, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24580529, Length=362, Percent_Identity=35.9116022099448, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24580537, Length=362, Percent_Identity=35.9116022099448, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24580533, Length=362, Percent_Identity=35.9116022099448, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24580535, Length=362, Percent_Identity=35.9116022099448, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24580531, Length=362, Percent_Identity=35.9116022099448, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24580527, Length=362, Percent_Identity=35.6353591160221, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24580525, Length=362, Percent_Identity=35.6353591160221, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24580541, Length=347, Percent_Identity=35.1585014409222, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42724; Mature: 42724

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00376 ADOMET_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITSESVKVGHPDIVADSIAANIIAAILDEEKKIGLTVDNMPHCGIEVFLGKGLCIVGG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEECCCEEEECC
EVATRIYVDIERCVRDTVLKLGYCDYELGLNGNSMGILNTIIPQSPDINIGTRADLGKYK
HHHEEHEEEHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHH
EIGAGDQGIIYGFACDETPELLPLPYVLATKMMRVFEEAKNPVFAPDGKGQITVEYNDEG
HCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCEEEEEECCCC
KPVRVATVLMSNAIDYRHVPEGFRNSIEPLAKQMAMNCFREWVDEQTDFLFNPTGEWQSV
CCEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
NSCSAADSGVTGRKLVVQLYGGYPGAQLGGGSIVNKTPEKVDCSAALGARYVAKNIVAAG
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
LAGKCSIQLSYAIGIARPISIYVHTFGTGVISDNKIGTIVREYFDLSPRGMIERFNLLDG
CCCCEEEEEEEEECCCCEEEEEEEECCCCEECCCHHHHHHHHHHCCCCCHHHHHHCCCCC
EKYRKLPKTLFLDDYPWEKTDMVDSLKKAADI
HHHHHCCHHEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQITSESVKVGHPDIVADSIAANIIAAILDEEKKIGLTVDNMPHCGIEVFLGKGLCIVGG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEECCCEEEECC
EVATRIYVDIERCVRDTVLKLGYCDYELGLNGNSMGILNTIIPQSPDINIGTRADLGKYK
HHHEEHEEEHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHH
EIGAGDQGIIYGFACDETPELLPLPYVLATKMMRVFEEAKNPVFAPDGKGQITVEYNDEG
HCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCEEEEEECCCC
KPVRVATVLMSNAIDYRHVPEGFRNSIEPLAKQMAMNCFREWVDEQTDFLFNPTGEWQSV
CCEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
NSCSAADSGVTGRKLVVQLYGGYPGAQLGGGSIVNKTPEKVDCSAALGARYVAKNIVAAG
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
LAGKCSIQLSYAIGIARPISIYVHTFGTGVISDNKIGTIVREYFDLSPRGMIERFNLLDG
CCCCEEEEEEEEECCCCEEEEEEEECCCCEECCCHHHHHHHHHHCCCCCHHHHHHCCCCC
EKYRKLPKTLFLDDYPWEKTDMVDSLKKAADI
HHHHHCCHHEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA