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The map label for this gene is galE [C]

Identifier: 85858957

GI number: 85858957

Start: 1137975

End: 1139018

Strand: Direct

Name: galE [C]

Synonym: SYN_00564

Alternate gene names: 85858957

Gene position: 1137975-1139018 (Clockwise)

Preceding gene: 85858953

Following gene: 85858958

Centisome position: 35.79

GC content: 58.43

Gene sequence:

>1044_bases
TTGATGAAGACCTTGTCTCCGGATTATCAAAATCGGAAAACGCGGTCAGCGTGGTGTTGCATGATAAAAAGAATACTGGT
TACCGGCGCCACGGGTCAGATCGGGTCTGAATTGGCCCTCGCTCTGAGACAGGCCTACGGTGAAGCCAAAGTCGTCGCTG
CAGGACACAGGCGCCGGCCTGATGCGGAGCTCCTGGAAAGCGGTCCTTACTGCAGTTTCGATGTCCGGGACGGTGAAACA
CTGCAGCGTATTGTGCAGGAGTACCGGATCGACACGATTTTTCATCTGGCATCGCTCCTGTCAGCTGCTGCGGAGAAGAA
CCCGCAATCTGCCTGGGAGATCAACATGAAGGGGCTGACCAATGTTCTGGAGACGGCCCGCATTTCCGGCTGCGCCGTTT
TCTTCCCCAGCTCCATCGGCGCCTTCGGTCCCGGCACTCCACTGGAAAACACACCCCAGCTGACCATTCAACGTCCTGCT
ACGCTCTACGGTATTACCAAACTGGCCGGCGAGCTGCTGTGCGACTATTATTTCCACCACTTCGGCGTCGACGCCCGCGG
TCTGCGCTTTCCGGGCCTTATTTCCTACAAGACGCCGCCCGGCGGCGGCACCACCGACTATGCCGTGGAGATCTTCACCG
CAGCCCTGACGGAAGGCCGCTACACCTGTTTTCTCAGGCCCGATACCCGGCTTGACATGATGTACATGCCGGACGCCATC
GGCGCGGCGATGATCCTGATGGAGGCGGACGGTCAAAGGCTCATTCACCGAAATGCCTATAACGTGACGGCCATGAACTT
CACGCCGGAGGAACTGGCGGTTCAGATTCTCCGGTTTGTGCCCGGTTTCGATATCCGCTATGATGTGGACCCGATCCGCC
AGGCCATTGCCGATTCCTGGCCCCGGCACATGGATGACAGCGCGGCGCGTACGGAATGGGGCTGGCAGCCCCGTTACGAC
CTTGCCGCCACGACATCGGAAATGATTGAACATCTTGCATTCAAACTGGGAAAAGCGGGGGAGGATCGACATGGCGCAGG
ATAA

Upstream 100 bases:

>100_bases
GCCTGCGCCCGGGACACGTCCCCTTATACCGTGTCTGTTGCGGTTATTATTTGATTGACACATCATAATGCTTTCCTTAT
ATCTTTCCGGTGTATAAAAG

Downstream 100 bases:

>100_bases
ACTGAAACGCCTGCTGGTGGAGAAGCTGGATGCGATGGAACGAAACGGCGTCCGCAAGGGGAAAGAAAGAATCATCACCG
GCCGCCGGCCGCCCCGCGAC

Product: L-threonine 3-dehydrogenase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MMKTLSPDYQNRKTRSAWCCMIKRILVTGATGQIGSELALALRQAYGEAKVVAAGHRRRPDAELLESGPYCSFDVRDGET
LQRIVQEYRIDTIFHLASLLSAAAEKNPQSAWEINMKGLTNVLETARISGCAVFFPSSIGAFGPGTPLENTPQLTIQRPA
TLYGITKLAGELLCDYYFHHFGVDARGLRFPGLISYKTPPGGGTTDYAVEIFTAALTEGRYTCFLRPDTRLDMMYMPDAI
GAAMILMEADGQRLIHRNAYNVTAMNFTPEELAVQILRFVPGFDIRYDVDPIRQAIADSWPRHMDDSAARTEWGWQPRYD
LAATTSEMIEHLAFKLGKAGEDRHGAG

Sequences:

>Translated_347_residues
MMKTLSPDYQNRKTRSAWCCMIKRILVTGATGQIGSELALALRQAYGEAKVVAAGHRRRPDAELLESGPYCSFDVRDGET
LQRIVQEYRIDTIFHLASLLSAAAEKNPQSAWEINMKGLTNVLETARISGCAVFFPSSIGAFGPGTPLENTPQLTIQRPA
TLYGITKLAGELLCDYYFHHFGVDARGLRFPGLISYKTPPGGGTTDYAVEIFTAALTEGRYTCFLRPDTRLDMMYMPDAI
GAAMILMEADGQRLIHRNAYNVTAMNFTPEELAVQILRFVPGFDIRYDVDPIRQAIADSWPRHMDDSAARTEWGWQPRYD
LAATTSEMIEHLAFKLGKAGEDRHGAG
>Mature_347_residues
MMKTLSPDYQNRKTRSAWCCMIKRILVTGATGQIGSELALALRQAYGEAKVVAAGHRRRPDAELLESGPYCSFDVRDGET
LQRIVQEYRIDTIFHLASLLSAAAEKNPQSAWEINMKGLTNVLETARISGCAVFFPSSIGAFGPGTPLENTPQLTIQRPA
TLYGITKLAGELLCDYYFHHFGVDARGLRFPGLISYKTPPGGGTTDYAVEIFTAALTEGRYTCFLRPDTRLDMMYMPDAI
GAAMILMEADGQRLIHRNAYNVTAMNFTPEELAVQILRFVPGFDIRYDVDPIRQAIADSWPRHMDDSAARTEWGWQPRYD
LAATTSEMIEHLAFKLGKAGEDRHGAG

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI32566934, Length=320, Percent_Identity=47.8125, Blast_Score=300, Evalue=6e-82,
Organism=Drosophila melanogaster, GI24667531, Length=307, Percent_Identity=49.185667752443, Blast_Score=303, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 38540; Mature: 38540

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKTLSPDYQNRKTRSAWCCMIKRILVTGATGQIGSELALALRQAYGEAKVVAAGHRRRP
CCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
DAELLESGPYCSFDVRDGETLQRIVQEYRIDTIFHLASLLSAAAEKNPQSAWEINMKGLT
CHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHH
NVLETARISGCAVFFPSSIGAFGPGTPLENTPQLTIQRPATLYGITKLAGELLCDYYFHH
HHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
FGVDARGLRFPGLISYKTPPGGGTTDYAVEIFTAALTEGRYTCFLRPDTRLDMMYMPDAI
HCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCHHH
GAAMILMEADGQRLIHRNAYNVTAMNFTPEELAVQILRFVPGFDIRYDVDPIRQAIADSW
HHEEEEEECCCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCC
PRHMDDSAARTEWGWQPRYDLAATTSEMIEHLAFKLGKAGEDRHGAG
CCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MMKTLSPDYQNRKTRSAWCCMIKRILVTGATGQIGSELALALRQAYGEAKVVAAGHRRRP
CCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
DAELLESGPYCSFDVRDGETLQRIVQEYRIDTIFHLASLLSAAAEKNPQSAWEINMKGLT
CHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHH
NVLETARISGCAVFFPSSIGAFGPGTPLENTPQLTIQRPATLYGITKLAGELLCDYYFHH
HHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
FGVDARGLRFPGLISYKTPPGGGTTDYAVEIFTAALTEGRYTCFLRPDTRLDMMYMPDAI
HCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCHHH
GAAMILMEADGQRLIHRNAYNVTAMNFTPEELAVQILRFVPGFDIRYDVDPIRQAIADSW
HHEEEEEECCCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCC
PRHMDDSAARTEWGWQPRYDLAATTSEMIEHLAFKLGKAGEDRHGAG
CCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA