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The map label for this gene is pilT [H]

Identifier: 85858697

GI number: 85858697

Start: 878372

End: 879586

Strand: Direct

Name: pilT [H]

Synonym: SYN_00126

Alternate gene names: 85858697

Gene position: 878372-879586 (Clockwise)

Preceding gene: 85858696

Following gene: 85858698

Centisome position: 27.63

GC content: 55.47

Gene sequence:

>1215_bases
TTGGAGAGCGTTCTCCCTACCCGTTTTTCAGGAGGCGTGAACATGCGAAGGCAGGAACTGGATTATATTTTGACGAAAAT
GCTGGATGCATACGACAATGTGTCCGATTTGAATATGACCGTGGGCAAACCCTTTCAGGTGGAGAGTTCCGGGCAGTTGA
CCGCTGTGGATTTTGATCCGCCCTGTGAAAAACTGACCCCCTTTCAGACGGAGACCATCGCCCTGAATCTGATCAACGGG
GACCGCCGCCTGACGGAAACTCTCGTTGCCGAGGGTTCCTGCGATTCCTCCTATACTCTGCAGGGTAAGGCCCGTTTCCG
GGTCAATATTTTTTCCCAGCGGGGAAATTATTCCATTGTCCTGCGGAAGCTGGAAACCCGCATTCCAACCCTTCAGGATC
TGAATCTGCCGGAGGCCTTCGAGAAAATGGCCCAGGAAAAAAACGGCATCATTCTCGTGACCGGGGCGACGGGAAGCGGA
AAGTCCACATCCCTGGCCGCGATGCTCAACGAGATCAACGAACGGAAGTCGGTCCATGTAGTGACCCTGGAAGACCCCGT
GGAATTCGTTCATCCACACAAGCAGGCGACCTTCAATCAACGGGAAATGGGGACGGATTTTGACACCTTTGCCAACGGCC
TCCGGGCGGCTCTCCGTCAGGCCCCCAAGGTGATCCTCGTCGGGGAAATGAGGGATCGGGAGACTGTTGAAATTGCCCTG
TCCGCTGCGGAGACCGGACATCTTGTCGTCAGCACCCTGCACACTGTGGATGCGGGGCAGACCATCAACCGCATTTTAGG
AATGTTTTCCATTGAGGAGGAGCGCCAGATCCGCATCCGTCTGGCCGATACGGTGCGCTGGATTGTATGCCAGCGGCTGC
TGCCCAAGGAGGGTGGGGGCCGGGTGGCGGCTTTTGAAGTGATGGGGAACAATCTGCGCGTGAAGGACACAATTCTGAAC
GGCGAATCGGAAGGCAAAACCTTTTATGAAATCATTCAGGCCGGCAAGGCCTTCGGGATGGTCACGTTCGATGATTACAT
CGTGGAGTTGTACCAGCGCGGCCTGGTCAGTGAAGAGACGGCCAAGGCCTATGCCTCCAACAAGGGCGTTGTGGGTCGAG
GGATCGATTCGGTCAAGAGCGCCAAGGGTGAGGCGACGACCAGCCTGGGTAAACTGGAGGTCGATACAAGCTATGGAAAA
CAGAAGAGGTTTTAA

Upstream 100 bases:

>100_bases
CAAATTCCGGCCGTTGTTGAAAAACCCGCCGACAGATTTTACGGAGGTCTAACCCTGGCGGTTATCCGTATGAACCGCGG
CACAAGAGATTCTTTCGGGA

Downstream 100 bases:

>100_bases
AAGTTCCAGCCGGCCGGATAAAGTTATTCTCATGGCATGTTCAGAGAATTCAGTCTGTTTACAACTGCACGACTTTGTCC
AAAATTCCGGCAAACAGGAC

Product: pili retraction protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MESVLPTRFSGGVNMRRQELDYILTKMLDAYDNVSDLNMTVGKPFQVESSGQLTAVDFDPPCEKLTPFQTETIALNLING
DRRLTETLVAEGSCDSSYTLQGKARFRVNIFSQRGNYSIVLRKLETRIPTLQDLNLPEAFEKMAQEKNGIILVTGATGSG
KSTSLAAMLNEINERKSVHVVTLEDPVEFVHPHKQATFNQREMGTDFDTFANGLRAALRQAPKVILVGEMRDRETVEIAL
SAAETGHLVVSTLHTVDAGQTINRILGMFSIEEERQIRIRLADTVRWIVCQRLLPKEGGGRVAAFEVMGNNLRVKDTILN
GESEGKTFYEIIQAGKAFGMVTFDDYIVELYQRGLVSEETAKAYASNKGVVGRGIDSVKSAKGEATTSLGKLEVDTSYGK
QKRF

Sequences:

>Translated_404_residues
MESVLPTRFSGGVNMRRQELDYILTKMLDAYDNVSDLNMTVGKPFQVESSGQLTAVDFDPPCEKLTPFQTETIALNLING
DRRLTETLVAEGSCDSSYTLQGKARFRVNIFSQRGNYSIVLRKLETRIPTLQDLNLPEAFEKMAQEKNGIILVTGATGSG
KSTSLAAMLNEINERKSVHVVTLEDPVEFVHPHKQATFNQREMGTDFDTFANGLRAALRQAPKVILVGEMRDRETVEIAL
SAAETGHLVVSTLHTVDAGQTINRILGMFSIEEERQIRIRLADTVRWIVCQRLLPKEGGGRVAAFEVMGNNLRVKDTILN
GESEGKTFYEIIQAGKAFGMVTFDDYIVELYQRGLVSEETAKAYASNKGVVGRGIDSVKSAKGEATTSLGKLEVDTSYGK
QKRF
>Mature_404_residues
MESVLPTRFSGGVNMRRQELDYILTKMLDAYDNVSDLNMTVGKPFQVESSGQLTAVDFDPPCEKLTPFQTETIALNLING
DRRLTETLVAEGSCDSSYTLQGKARFRVNIFSQRGNYSIVLRKLETRIPTLQDLNLPEAFEKMAQEKNGIILVTGATGSG
KSTSLAAMLNEINERKSVHVVTLEDPVEFVHPHKQATFNQREMGTDFDTFANGLRAALRQAPKVILVGEMRDRETVEIAL
SAAETGHLVVSTLHTVDAGQTINRILGMFSIEEERQIRIRLADTVRWIVCQRLLPKEGGGRVAAFEVMGNNLRVKDTILN
GESEGKTFYEIIQAGKAFGMVTFDDYIVELYQRGLVSEETAKAYASNKGVVGRGIDSVKSAKGEATTSLGKLEVDTSYGK
QKRF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=318, Percent_Identity=40.5660377358491, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789723, Length=156, Percent_Identity=44.2307692307692, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786296, Length=213, Percent_Identity=37.5586854460094, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 44731; Mature: 44731

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESVLPTRFSGGVNMRRQELDYILTKMLDAYDNVSDLNMTVGKPFQVESSGQLTAVDFDP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCEEEEECCC
PCEKLTPFQTETIALNLINGDRRLTETLVAEGSCDSSYTLQGKARFRVNIFSQRGNYSIV
CHHHCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCEEEECCEEEEEEEEECCCCEEEE
LRKLETRIPTLQDLNLPEAFEKMAQEKNGIILVTGATGSGKSTSLAAMLNEINERKSVHV
EEEHHHHCCCHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
VTLEDPVEFVHPHKQATFNQREMGTDFDTFANGLRAALRQAPKVILVGEMRDRETVEIAL
EEECCCHHHHCCCHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEE
SAAETGHLVVSTLHTVDAGQTINRILGMFSIEEERQIRIRLADTVRWIVCQRLLPKEGGG
ECCCCCCEEEEEHHHCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCC
RVAAFEVMGNNLRVKDTILNGESEGKTFYEIIQAGKAFGMVTFDDYIVELYQRGLVSEET
EEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCCHHH
AKAYASNKGVVGRGIDSVKSAKGEATTSLGKLEVDTSYGKQKRF
HHHHHCCCCCEECCCHHHHHCCCCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MESVLPTRFSGGVNMRRQELDYILTKMLDAYDNVSDLNMTVGKPFQVESSGQLTAVDFDP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCEEEEECCC
PCEKLTPFQTETIALNLINGDRRLTETLVAEGSCDSSYTLQGKARFRVNIFSQRGNYSIV
CHHHCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCEEEECCEEEEEEEEECCCCEEEE
LRKLETRIPTLQDLNLPEAFEKMAQEKNGIILVTGATGSGKSTSLAAMLNEINERKSVHV
EEEHHHHCCCHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
VTLEDPVEFVHPHKQATFNQREMGTDFDTFANGLRAALRQAPKVILVGEMRDRETVEIAL
EEECCCHHHHCCCHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEE
SAAETGHLVVSTLHTVDAGQTINRILGMFSIEEERQIRIRLADTVRWIVCQRLLPKEGGG
ECCCCCCEEEEEHHHCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCC
RVAAFEVMGNNLRVKDTILNGESEGKTFYEIIQAGKAFGMVTFDDYIVELYQRGLVSEET
EEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCCHHH
AKAYASNKGVVGRGIDSVKSAKGEATTSLGKLEVDTSYGKQKRF
HHHHHCCCCCEECCCHHHHHCCCCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]