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The map label for this gene is pilT [H]

Identifier: 85858696

GI number: 85858696

Start: 877241

End: 878323

Strand: Direct

Name: pilT [H]

Synonym: SYN_00125

Alternate gene names: 85858696

Gene position: 877241-878323 (Clockwise)

Preceding gene: 85858695

Following gene: 85858697

Centisome position: 27.59

GC content: 55.03

Gene sequence:

>1083_bases
ATGGCCAAAATCGATGCTTTTTTCCAGTTGATGCACGAACAGGGCGCATCGGACCTGCATCTCGTTTCCGGACAGCAGCC
TGCCATCCGGATGCGCGGTGAACTGGAGCGGGTGAAATATAATATTCTGGATAACGATGAGCTGAAGGCGATGCTCTATG
AAATCACGCCGGAACACAAAATCAAGCAGTTCGAGGAAACGGGGGATGTCGATTTTGCCTATGAAATTCCTGGTCTGGCC
CGTTACCGCGCCAATTTTTTCGAACAGAAGTTCGGTGTGGCAGCCGTTTTCCGGGAAATTCCCAGCCAGATCCTGACCTG
TGAGCAACTGGGACTGCCGCCCGTAGTTTCCAAACTGGCCACGCTGCCCCGGGGGCTGGTTCTGGTGACCGGACCAACAG
GCAGTGGAAAGTCAACGACCCTGGCAGCCATCATCGATCAAGCCAACCGCCTGCGGAAGGATCACATCATCACCGTCGAA
GATCCCATTGAATTCGTTCATCAGAGCCAGAGCTGCATCGTCAACCATCGGGAAGTCGGCATTCATACAAAGAGCTTTTC
CGCGGCCCTGAGAGGCGCCCTGCGTGAAGACCCGGACATCATACTGGTCGGGGAAATGCGGGATCTGGAAACCATCTCCC
TGGCTGTGGAAGCCGCCTCCACCGGCCATCTCGTTTTCGGGACCCTTCATACCACGAGTGCGGCGAAAACAGTTGACCGG
ATCATCGAGGTGTTTCCTTCCAGTGAGCAGGCCCAGATCCGATCCACCCTGGCAGACGGTCTGAGGGCGATCATCGCCCA
GGTGCTCTTCAAGAGGATCGACAGAAAGGGCCGATGCGCTGCTCTGGAAATCCTCATAGCCAATGCGGCCGTGCGCAATC
TGATCCGCGAGTCAAAGACCTTCCAGATTCCATCCATGATCCAGACGGGCAAGAAGTACGGGATGCAGCTTCTCGATGAC
GCCATCATGGAACTGTTCAATAAGGGATGGATCAGCGCCGATGAAGCCTACATGAAGTGCAACGACAAGACCAAATTCCG
GCCGTTGTTGAAAAACCCGCCGACAGATTTTACGGAGGTCTAA

Upstream 100 bases:

>100_bases
TTTCGTCCTTACCCGGGAAGAAAGGCCGTTTTTTACGAAAAGTATGTTGTGATATAAGAGTGGAAATCAGATCAATTTCA
ACTGAACAAGGAGACTGTGT

Downstream 100 bases:

>100_bases
CCCTGGCGGTTATCCGTATGAACCGCGGCACAAGAGATTCTTTCGGGATTGGAGAGCGTTCTCCCTACCCGTTTTTCAGG
AGGCGTGAACATGCGAAGGC

Product: pili retraction protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MAKIDAFFQLMHEQGASDLHLVSGQQPAIRMRGELERVKYNILDNDELKAMLYEITPEHKIKQFEETGDVDFAYEIPGLA
RYRANFFEQKFGVAAVFREIPSQILTCEQLGLPPVVSKLATLPRGLVLVTGPTGSGKSTTLAAIIDQANRLRKDHIITVE
DPIEFVHQSQSCIVNHREVGIHTKSFSAALRGALREDPDIILVGEMRDLETISLAVEAASTGHLVFGTLHTTSAAKTVDR
IIEVFPSSEQAQIRSTLADGLRAIIAQVLFKRIDRKGRCAALEILIANAAVRNLIRESKTFQIPSMIQTGKKYGMQLLDD
AIMELFNKGWISADEAYMKCNDKTKFRPLLKNPPTDFTEV

Sequences:

>Translated_360_residues
MAKIDAFFQLMHEQGASDLHLVSGQQPAIRMRGELERVKYNILDNDELKAMLYEITPEHKIKQFEETGDVDFAYEIPGLA
RYRANFFEQKFGVAAVFREIPSQILTCEQLGLPPVVSKLATLPRGLVLVTGPTGSGKSTTLAAIIDQANRLRKDHIITVE
DPIEFVHQSQSCIVNHREVGIHTKSFSAALRGALREDPDIILVGEMRDLETISLAVEAASTGHLVFGTLHTTSAAKTVDR
IIEVFPSSEQAQIRSTLADGLRAIIAQVLFKRIDRKGRCAALEILIANAAVRNLIRESKTFQIPSMIQTGKKYGMQLLDD
AIMELFNKGWISADEAYMKCNDKTKFRPLLKNPPTDFTEV
>Mature_359_residues
AKIDAFFQLMHEQGASDLHLVSGQQPAIRMRGELERVKYNILDNDELKAMLYEITPEHKIKQFEETGDVDFAYEIPGLAR
YRANFFEQKFGVAAVFREIPSQILTCEQLGLPPVVSKLATLPRGLVLVTGPTGSGKSTTLAAIIDQANRLRKDHIITVED
PIEFVHQSQSCIVNHREVGIHTKSFSAALRGALREDPDIILVGEMRDLETISLAVEAASTGHLVFGTLHTTSAAKTVDRI
IEVFPSSEQAQIRSTLADGLRAIIAQVLFKRIDRKGRCAALEILIANAAVRNLIRESKTFQIPSMIQTGKKYGMQLLDDA
IMELFNKGWISADEAYMKCNDKTKFRPLLKNPPTDFTEV

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=300, Percent_Identity=46, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI1789723, Length=297, Percent_Identity=35.016835016835, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786296, Length=282, Percent_Identity=33.6879432624113, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40120; Mature: 39989

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIDAFFQLMHEQGASDLHLVSGQQPAIRMRGELERVKYNILDNDELKAMLYEITPEHK
CCHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCCHHHHEEECCCCHHHHHHHHHCCCHHH
IKQFEETGDVDFAYEIPGLARYRANFFEQKFGVAAVFREIPSQILTCEQLGLPPVVSKLA
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TLPRGLVLVTGPTGSGKSTTLAAIIDQANRLRKDHIITVEDPIEFVHQSQSCIVNHREVG
HCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEECHHHHHHCCHHHHCCHHHHC
IHTKSFSAALRGALREDPDIILVGEMRDLETISLAVEAASTGHLVFGTLHTTSAAKTVDR
CCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHEEEEECCCCCEEEEEEHHHHHHHHHHH
IIEVFPSSEQAQIRSTLADGLRAIIAQVLFKRIDRKGRCAALEILIANAAVRNLIRESKT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
FQIPSMIQTGKKYGMQLLDDAIMELFNKGWISADEAYMKCNDKTKFRPLLKNPPTDFTEV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
AKIDAFFQLMHEQGASDLHLVSGQQPAIRMRGELERVKYNILDNDELKAMLYEITPEHK
CHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCCHHHHEEECCCCHHHHHHHHHCCCHHH
IKQFEETGDVDFAYEIPGLARYRANFFEQKFGVAAVFREIPSQILTCEQLGLPPVVSKLA
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TLPRGLVLVTGPTGSGKSTTLAAIIDQANRLRKDHIITVEDPIEFVHQSQSCIVNHREVG
HCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEECHHHHHHCCHHHHCCHHHHC
IHTKSFSAALRGALREDPDIILVGEMRDLETISLAVEAASTGHLVFGTLHTTSAAKTVDR
CCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHEEEEECCCCCEEEEEEHHHHHHHHHHH
IIEVFPSSEQAQIRSTLADGLRAIIAQVLFKRIDRKGRCAALEILIANAAVRNLIRESKT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
FQIPSMIQTGKKYGMQLLDDAIMELFNKGWISADEAYMKCNDKTKFRPLLKNPPTDFTEV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]