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The map label for this gene is nreB [H]

Identifier: 85858633

GI number: 85858633

Start: 804483

End: 806042

Strand: Direct

Name: nreB [H]

Synonym: SYN_01954

Alternate gene names: 85858633

Gene position: 804483-806042 (Clockwise)

Preceding gene: 85858632

Following gene: 85858634

Centisome position: 25.3

GC content: 55.58

Gene sequence:

>1560_bases
TTGATTGAAGAAGGGGATAAAGCGGAGGAAAGGGACATGGAATATCCGGACAGCAACTCTGAAATGTCGGAAGTACAGCT
TCTGCAAACCCTTTCCGATCTTCGGGAAAAGATGGCCGCCCTGGAAAAGGAGCGCAGCGAACATCTCCTCGAACAGGACC
TTTACAAGACACTGGCCAACAGTTCGCAGGTGGGCGTCTATATCCTCCAGGATCGGAAGTTTCAGTTCGTCAACCCCCAT
ATTGCCGAATACGCCGGGTATCGGGAAGAAGAAATGGTAGGAATGGAATCCCTCAGCCTCATCCATCCCGATGACCGTCT
GACGGCCCGGAAAAACGCCATCCGCATGCTGAAGGGGCAGCGCTTTTCGCCGTACGAATTCCGCATCATTACCGGTGATG
GCCGGATCAAATGGATCATGGAAACTTCGACGCCCATCTATTACAGGGGTAAACGCGCCGTTCTGGGAAATTCCATGAAC
ATCACCGGGCAGAAAGAAGCCCGCAACCGGCTGGAGGAACTGGAAGCGCTGGAGTCTTCTATCCTGGACGCCATCCCGCA
TGCGGTGGTCGGTCTGCACAACCGCCGGTTCATCTTTGCCAACAACGCCGTTCAGGATGTGTTCGGCTGGCTGCCGGAAG
AGCTGATCGGCAGGAGCGTCCGGCTGATCTATCGCAATGACGCGGACCATGATGAAATCGCGCGGCGTTTTTACGACAGC
CTGGAAAAACAGCGGACATACAGCACGGAATTTCCCTGCCGGCACAAGGATGGTCATGAGATCATCTGTTTGATGAGGGC
TTCCCGCATCGGGGAACGGCTGACCGAACGGCGGATTGTCGTCACTTACGAGGACATTACGGAACGGAAAAAGGCGGAAA
AGGAGCTGGAGGAGTCGCGGGAAAAAATGCGCAACCTTTCCATTCACCTTCAGTCTGTCCGTGAGGAAGAACGGACACGG
ATCGCCCGGGAGATACACGATGAACTGGGGCAGTCGCTGACCGCCTTTAAAATGGATCTGTCCTGGCTGGGGAAAAGGAT
GACCTCGGAGAAACTGCTGCACGATAAGATCAAAGCCATGTCGGGACTCGTGGATCGGACCATTGAATCGGTCCATCGGA
TTTCGGCGGACCTCAGGCCCGGCCTGCTGGATGACCTGGGGCTTGTGGCGGCGATGGAATGGCAGGCCAAGGAGTTTTCC
GCTCGTTCCGGCATTGCCTGCGAGGCGGATCTGGAAGCGGAAGATGTCCCTCTGGATAAGGAACCGGCCACGGCCGTTTT
CCGCATATTTCAGGAAACACTGACCAATGTTGCCCGTCACTCCAATGCCACAAAGGTATTTGTGCGTCTGGAGACGAAGG
GCGACAAGGTGATCCTGGAGGTGACGGACAATGGCCGGGGCATCACGCAGAAACAGATCAACGATCCGAAATCGTTCGGA
ATTATGGGAATGCGGGAACGTGCCCTGCTCTGGGGAGGAGACGTTCAGGTGATCGGCAGCCGGTCCAGAGGAACAACGGT
GAAGGTCAGCATTCCCCTGAAGTCAGGAGAGAAGGAATGA

Upstream 100 bases:

>100_bases
GCGGCGAAGCCTCGGGCTGTGATCCCCTGCAGAGCGTTCTGGGGATGACTGTTCTGCTGCCTGATGTCACGACGCCGTCG
ATCCCGGCGCCGAAGCCGAA

Downstream 100 bases:

>100_bases
TTAAAATCCTGATTGCCGATGATCATACCATCGTCAGGGAAGGGTTGAAACAGATTGTGGGAGACGTCGGCGACATGATG
GTGGCCGATGAGGCCGGCAA

Product: signal transduction histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MIEEGDKAEERDMEYPDSNSEMSEVQLLQTLSDLREKMAALEKERSEHLLEQDLYKTLANSSQVGVYILQDRKFQFVNPH
IAEYAGYREEEMVGMESLSLIHPDDRLTARKNAIRMLKGQRFSPYEFRIITGDGRIKWIMETSTPIYYRGKRAVLGNSMN
ITGQKEARNRLEELEALESSILDAIPHAVVGLHNRRFIFANNAVQDVFGWLPEELIGRSVRLIYRNDADHDEIARRFYDS
LEKQRTYSTEFPCRHKDGHEIICLMRASRIGERLTERRIVVTYEDITERKKAEKELEESREKMRNLSIHLQSVREEERTR
IAREIHDELGQSLTAFKMDLSWLGKRMTSEKLLHDKIKAMSGLVDRTIESVHRISADLRPGLLDDLGLVAAMEWQAKEFS
ARSGIACEADLEAEDVPLDKEPATAVFRIFQETLTNVARHSNATKVFVRLETKGDKVILEVTDNGRGITQKQINDPKSFG
IMGMRERALLWGGDVQVIGSRSRGTTVKVSIPLKSGEKE

Sequences:

>Translated_519_residues
MIEEGDKAEERDMEYPDSNSEMSEVQLLQTLSDLREKMAALEKERSEHLLEQDLYKTLANSSQVGVYILQDRKFQFVNPH
IAEYAGYREEEMVGMESLSLIHPDDRLTARKNAIRMLKGQRFSPYEFRIITGDGRIKWIMETSTPIYYRGKRAVLGNSMN
ITGQKEARNRLEELEALESSILDAIPHAVVGLHNRRFIFANNAVQDVFGWLPEELIGRSVRLIYRNDADHDEIARRFYDS
LEKQRTYSTEFPCRHKDGHEIICLMRASRIGERLTERRIVVTYEDITERKKAEKELEESREKMRNLSIHLQSVREEERTR
IAREIHDELGQSLTAFKMDLSWLGKRMTSEKLLHDKIKAMSGLVDRTIESVHRISADLRPGLLDDLGLVAAMEWQAKEFS
ARSGIACEADLEAEDVPLDKEPATAVFRIFQETLTNVARHSNATKVFVRLETKGDKVILEVTDNGRGITQKQINDPKSFG
IMGMRERALLWGGDVQVIGSRSRGTTVKVSIPLKSGEKE
>Mature_519_residues
MIEEGDKAEERDMEYPDSNSEMSEVQLLQTLSDLREKMAALEKERSEHLLEQDLYKTLANSSQVGVYILQDRKFQFVNPH
IAEYAGYREEEMVGMESLSLIHPDDRLTARKNAIRMLKGQRFSPYEFRIITGDGRIKWIMETSTPIYYRGKRAVLGNSMN
ITGQKEARNRLEELEALESSILDAIPHAVVGLHNRRFIFANNAVQDVFGWLPEELIGRSVRLIYRNDADHDEIARRFYDS
LEKQRTYSTEFPCRHKDGHEIICLMRASRIGERLTERRIVVTYEDITERKKAEKELEESREKMRNLSIHLQSVREEERTR
IAREIHDELGQSLTAFKMDLSWLGKRMTSEKLLHDKIKAMSGLVDRTIESVHRISADLRPGLLDDLGLVAAMEWQAKEFS
ARSGIACEADLEAEDVPLDKEPATAVFRIFQETLTNVARHSNATKVFVRLETKGDKVILEVTDNGRGITQKQINDPKSFG
IMGMRERALLWGGDVQVIGSRSRGTTVKVSIPLKSGEKE

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=239, Percent_Identity=30.5439330543933, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082311, Length=232, Percent_Identity=26.7241379310345, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788812, Length=213, Percent_Identity=26.2910798122066, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59614; Mature: 59614

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEEGDKAEERDMEYPDSNSEMSEVQLLQTLSDLREKMAALEKERSEHLLEQDLYKTLAN
CCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SSQVGVYILQDRKFQFVNPHIAEYAGYREEEMVGMESLSLIHPDDRLTARKNAIRMLKGQ
CCCCEEEEEECCCEEECCCHHHHHCCCCHHHHHCHHHHEEECCCHHHHHHHHHHHHHCCC
RFSPYEFRIITGDGRIKWIMETSTPIYYRGKRAVLGNSMNITGQKEARNRLEELEALESS
CCCCEEEEEEECCCEEEEEEECCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
ILDAIPHAVVGLHNRRFIFANNAVQDVFGWLPEELIGRSVRLIYRNDADHDEIARRFYDS
HHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCHHHCCCCEEEEEECCCCHHHHHHHHHHH
LEKQRTYSTEFPCRHKDGHEIICLMRASRIGERLTERRIVVTYEDITERKKAEKELEESR
HHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHH
EKMRNLSIHLQSVREEERTRIAREIHDELGQSLTAFKMDLSWLGKRMTSEKLLHDKIKAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLVDRTIESVHRISADLRPGLLDDLGLVAAMEWQAKEFSARSGIACEADLEAEDVPLDK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
EPATAVFRIFQETLTNVARHSNATKVFVRLETKGDKVILEVTDNGRGITQKQINDPKSFG
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHCCCCHHCC
IMGMRERALLWGGDVQVIGSRSRGTTVKVSIPLKSGEKE
CCCCCCCEEEECCCEEEEECCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MIEEGDKAEERDMEYPDSNSEMSEVQLLQTLSDLREKMAALEKERSEHLLEQDLYKTLAN
CCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SSQVGVYILQDRKFQFVNPHIAEYAGYREEEMVGMESLSLIHPDDRLTARKNAIRMLKGQ
CCCCEEEEEECCCEEECCCHHHHHCCCCHHHHHCHHHHEEECCCHHHHHHHHHHHHHCCC
RFSPYEFRIITGDGRIKWIMETSTPIYYRGKRAVLGNSMNITGQKEARNRLEELEALESS
CCCCEEEEEEECCCEEEEEEECCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
ILDAIPHAVVGLHNRRFIFANNAVQDVFGWLPEELIGRSVRLIYRNDADHDEIARRFYDS
HHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCHHHCCCCEEEEEECCCCHHHHHHHHHHH
LEKQRTYSTEFPCRHKDGHEIICLMRASRIGERLTERRIVVTYEDITERKKAEKELEESR
HHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHH
EKMRNLSIHLQSVREEERTRIAREIHDELGQSLTAFKMDLSWLGKRMTSEKLLHDKIKAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLVDRTIESVHRISADLRPGLLDDLGLVAAMEWQAKEFSARSGIACEADLEAEDVPLDK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
EPATAVFRIFQETLTNVARHSNATKVFVRLETKGDKVILEVTDNGRGITQKQINDPKSFG
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHCCCCHHCC
IMGMRERALLWGGDVQVIGSRSRGTTVKVSIPLKSGEKE
CCCCCCCEEEECCCEEEEECCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]