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The map label for this gene is gacA [H]

Identifier: 85858634

GI number: 85858634

Start: 806039

End: 806671

Strand: Direct

Name: gacA [H]

Synonym: SYN_01953

Alternate gene names: 85858634

Gene position: 806039-806671 (Clockwise)

Preceding gene: 85858633

Following gene: 85858637

Centisome position: 25.35

GC content: 55.45

Gene sequence:

>633_bases
ATGATTAAAATCCTGATTGCCGATGATCATACCATCGTCAGGGAAGGGTTGAAACAGATTGTGGGAGACGTCGGCGACAT
GATGGTGGCCGATGAGGCCGGCAACGGACAGGAAGCACTGCAGAAAATTCGGGAAAAGGATTATGATGTCGTCCTGCTGG
ATATTTCCATGCCGGGGCGCAGCGGCCTGGAAGTTCTGAAGGATATACGGGCGGAGCGGCCGAAACTGCCCGTCCTGATC
CTGAGCATGCATTCCGAAGAACAGTATGCCGTCCGGGCGCTCCGGGCCGGCGCGTCGGGCTATCTGACGAAGGCGAGCGC
TCCCGATGAGCTGATCGGGGCGATCCGCAAGGTATCCCGGGGGCGCAAATACGTAACGGCATCGCTGGCGGAAAAACTGG
CTCTGGAACTGGATGCCGATATCCGGAAACCGCCCCACGAGATACTTTCCGATCGGGAATATCAGGTCATGCTCATGCTG
GCTTCAGGGAAAACCGTCACGGAAATCGCCGAAGAGCTGTGTCTCAGCGTAAAGACCATCAGCACGTATCGCTCCCGGAT
TCTGGAAAAAATGAACATGAAAAAAAACGCGGAGCTGACACTTTACGCGGTTCAGAATCACCTTGTGGACTGA

Upstream 100 bases:

>100_bases
AACGTGCCCTGCTCTGGGGAGGAGACGTTCAGGTGATCGGCAGCCGGTCCAGAGGAACAACGGTGAAGGTCAGCATTCCC
CTGAAGTCAGGAGAGAAGGA

Downstream 100 bases:

>100_bases
TGCTCCGCCGACCGCTTCAGCGAAATTCGCCGTTTGCGCCATCCGCGCATGTGGCGTGATTGCGCGACGTGTTTCTGCTT
TTTCTCTATCCCGTTCAGTG

Product: response regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIKILIADDHTIVREGLKQIVGDVGDMMVADEAGNGQEALQKIREKDYDVVLLDISMPGRSGLEVLKDIRAERPKLPVLI
LSMHSEEQYAVRALRAGASGYLTKASAPDELIGAIRKVSRGRKYVTASLAEKLALELDADIRKPPHEILSDREYQVMLML
ASGKTVTEIAEELCLSVKTISTYRSRILEKMNMKKNAELTLYAVQNHLVD

Sequences:

>Translated_210_residues
MIKILIADDHTIVREGLKQIVGDVGDMMVADEAGNGQEALQKIREKDYDVVLLDISMPGRSGLEVLKDIRAERPKLPVLI
LSMHSEEQYAVRALRAGASGYLTKASAPDELIGAIRKVSRGRKYVTASLAEKLALELDADIRKPPHEILSDREYQVMLML
ASGKTVTEIAEELCLSVKTISTYRSRILEKMNMKKNAELTLYAVQNHLVD
>Mature_210_residues
MIKILIADDHTIVREGLKQIVGDVGDMMVADEAGNGQEALQKIREKDYDVVLLDISMPGRSGLEVLKDIRAERPKLPVLI
LSMHSEEQYAVRALRAGASGYLTKASAPDELIGAIRKVSRGRKYVTASLAEKLALELDADIRKPPHEILSDREYQVMLML
ASGKTVTEIAEELCLSVKTISTYRSRILEKMNMKKNAELTLYAVQNHLVD

Specific function: Forms part of a two-component regulatory system gacA/gacA(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL) [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=38.3886255924171, Blast_Score=171, Evalue=3e-44,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=41, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=31.7073170731707, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=29.4117647058824, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1786747, Length=200, Percent_Identity=27.5, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=31.3186813186813, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788546, Length=207, Percent_Identity=28.5024154589372, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1790299, Length=114, Percent_Identity=35.9649122807018, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788191, Length=105, Percent_Identity=36.1904761904762, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1790437, Length=85, Percent_Identity=40, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=37.6146788990826, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI87082012, Length=181, Percent_Identity=28.1767955801105, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786911, Length=127, Percent_Identity=33.0708661417323, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=30.2521008403361, Blast_Score=60, Evalue=7e-11,
Organism=Escherichia coli, GI1787375, Length=156, Percent_Identity=26.9230769230769, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23360; Mature: 23360

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKILIADDHTIVREGLKQIVGDVGDMMVADEAGNGQEALQKIREKDYDVVLLDISMPGR
CEEEEEECCHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCC
SGLEVLKDIRAERPKLPVLILSMHSEEQYAVRALRAGASGYLTKASAPDELIGAIRKVSR
CHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHC
GRKYVTASLAEKLALELDADIRKPPHEILSDREYQVMLMLASGKTVTEIAEELCLSVKTI
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
STYRSRILEKMNMKKNAELTLYAVQNHLVD
HHHHHHHHHHHCCCCCCCEEEEEECHHCCC
>Mature Secondary Structure
MIKILIADDHTIVREGLKQIVGDVGDMMVADEAGNGQEALQKIREKDYDVVLLDISMPGR
CEEEEEECCHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCC
SGLEVLKDIRAERPKLPVLILSMHSEEQYAVRALRAGASGYLTKASAPDELIGAIRKVSR
CHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHC
GRKYVTASLAEKLALELDADIRKPPHEILSDREYQVMLMLASGKTVTEIAEELCLSVKTI
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
STYRSRILEKMNMKKNAELTLYAVQNHLVD
HHHHHHHHHHHCCCCCCCEEEEEECHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002569 [H]