Click here to switch to the map view.

The map label for this gene is ptsI [H]

Identifier: 85858509

GI number: 85858509

Start: 662274

End: 664058

Strand: Direct

Name: ptsI [H]

Synonym: SYN_01729

Alternate gene names: 85858509

Gene position: 662274-664058 (Clockwise)

Preceding gene: 85858508

Following gene: 85858511

Centisome position: 20.83

GC content: 48.35

Gene sequence:

>1785_bases
ATGAATACAACGGATGAAATCAAAAAAACTTTCGTATTGAAGGGTGTTGGTGTTTCGCCGGGTATCGCCTCTGGCAAGGC
TTTTCTTTTCGATCCTCTGGGGAGTGAGGTCTCGTTTTACAAGTTGAGTGATAAGAGTCTCATTACCAAGGAGATCAGAC
GTTTCAAGGATGCCCTGAAGGAATCGGAGCGGCAGCTTCTTGAAATAAAAAATGGAATTCATGGAGTTATCGGTCCGGAA
CCTCTGTATATCATTGATGTCCATATTATGCTTTTACGGGATAAGGCCTTTATCAGGCACACCGTTGAGCATATCCGGCA
ATCGTGCGTCAATGCCGAATGGGCGGTTCAAATGACCACGGAAAAATACCGTGAATTGTTTGACCGCGTGGAGGATGAAT
ATATCCGGAGTCGCTTCAGTGATATCCAGTATGTGGGGAAAATGATTCTCCGCAATCTTGCCGGAAAAAAAAGGGGGCCC
TTCTCGGAAATCAGCGAAGAGGTCATTGTGGTATCTTCTGATCTTTCTCCCGCGGATACGGCCCAGATGATGGTTCACAA
GGTCCTGGGTTTTGCTACGGATATGGGCGGTAAAACATCGCATACCGCCATCGTAGCCCGCTCGATTGCAATTCCTGCCG
TTGTGGGTTTGAAAAACATTACCCGCGTCGTTAAAACCAATGACGATTTGATCATTGACGGAGCCTCCGGTTTGATTATC
GTCAATCCCGACCCGGAAGTTGCCCGACGGTATCAGGAGAAAAAGAGACAATATGAAACCATACAGGAGGATTTTCTGAA
ATATGCCCATCTTCCTGCCATGACGAAAGATCACTACCGGGTTGATATCGGCAGCAATATCGAATTTATCGAAGAAATCC
CGTCGGCGGTTCTTCACGGCGCTGAAGGCATCGGTCTTTATCGGACGGAATTCATTTATATCAATCGAGATCAGTTGCCG
ACGGAGGAGGAACATTTTGCACATTACCATCAGGTGGTCGGAGTCGAGGGGTTAAGATGGTCGACGATTCGAACCTTCGA
TCTGGGAGGCGACAAGTTTACCTCCGATCCGAAGCTGGCAAAAGAAATGAACCCTCAGTTGGGATTGCGGGCGATTCGTT
TCTGCCTGAAGGAAGTGGAGCAGTTCAAGGTTCAATTGCGCGCGATCATACGCGCAAGCGCTTATGGTAAAACCCGCATC
ATGTTTCCCATGATTTCAGGTATCGAAGAAATCCGCGAAGCCAAACGAATTTTCAACGATGTCATGCGCGATCTGATTGA
AGAAGACATCCCCGTAGGCCGTGATATTGAAATCGGGGTCATGATTGAAGTGCCGTCCGCAGGCATTGTTGCGGATGAGT
TGGCGAAAGAAGTGGATTTCTTCAGCATTGGCACCAACGATCTCATTCAGTATGTTCTGGCGATCGACCGGATCAATGAA
CGTGTCGGTTATCTATACGAGCCCCTCCATCCCGCCGTATTGAGATTGATCCAGCAAATTGTCAGCGCTGGTCACCGAAA
TGGCATCCGGGTAGAGATGTGCGGCGAGATGGCTGGAGAACCCCTCTATGCACTGATTCTTCTCGGTCTTGAACTGGATG
AACTGAGTATGAATCCCCTGGCGATTCCTCGAATGAAAAAGATCATCCAGAATTCCACGTTGAAGGAATCCCGCGAACTG
CTGGATAAAGTCCTGATATTGCACTCCGCGACGGAGATCCGCGACTACGTGGAACATTATATGAGGCAGCGATTTCCGGA
TGAGTTTCCTGAGAATGGACCATAG

Upstream 100 bases:

>100_bases
GCCCTCAGGGGAGCCGGGTAACTGTACGGGTGGAGGGTGCCGATGCTCTTCAGGCTATGGATAATCTGGAGCAGCTTTTC
GAGAGTAAATTTGGTGAAAC

Downstream 100 bases:

>100_bases
AACCAGCATGCTTGTCCGGGAATATCGGCTTGTTTTAAATTGAACAGATAGTAACCGGTCCTGCAAGGAAGAGGACTGTT
TTCATCTGCCTGCCTGTAAT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MNTTDEIKKTFVLKGVGVSPGIASGKAFLFDPLGSEVSFYKLSDKSLITKEIRRFKDALKESERQLLEIKNGIHGVIGPE
PLYIIDVHIMLLRDKAFIRHTVEHIRQSCVNAEWAVQMTTEKYRELFDRVEDEYIRSRFSDIQYVGKMILRNLAGKKRGP
FSEISEEVIVVSSDLSPADTAQMMVHKVLGFATDMGGKTSHTAIVARSIAIPAVVGLKNITRVVKTNDDLIIDGASGLII
VNPDPEVARRYQEKKRQYETIQEDFLKYAHLPAMTKDHYRVDIGSNIEFIEEIPSAVLHGAEGIGLYRTEFIYINRDQLP
TEEEHFAHYHQVVGVEGLRWSTIRTFDLGGDKFTSDPKLAKEMNPQLGLRAIRFCLKEVEQFKVQLRAIIRASAYGKTRI
MFPMISGIEEIREAKRIFNDVMRDLIEEDIPVGRDIEIGVMIEVPSAGIVADELAKEVDFFSIGTNDLIQYVLAIDRINE
RVGYLYEPLHPAVLRLIQQIVSAGHRNGIRVEMCGEMAGEPLYALILLGLELDELSMNPLAIPRMKKIIQNSTLKESREL
LDKVLILHSATEIRDYVEHYMRQRFPDEFPENGP

Sequences:

>Translated_594_residues
MNTTDEIKKTFVLKGVGVSPGIASGKAFLFDPLGSEVSFYKLSDKSLITKEIRRFKDALKESERQLLEIKNGIHGVIGPE
PLYIIDVHIMLLRDKAFIRHTVEHIRQSCVNAEWAVQMTTEKYRELFDRVEDEYIRSRFSDIQYVGKMILRNLAGKKRGP
FSEISEEVIVVSSDLSPADTAQMMVHKVLGFATDMGGKTSHTAIVARSIAIPAVVGLKNITRVVKTNDDLIIDGASGLII
VNPDPEVARRYQEKKRQYETIQEDFLKYAHLPAMTKDHYRVDIGSNIEFIEEIPSAVLHGAEGIGLYRTEFIYINRDQLP
TEEEHFAHYHQVVGVEGLRWSTIRTFDLGGDKFTSDPKLAKEMNPQLGLRAIRFCLKEVEQFKVQLRAIIRASAYGKTRI
MFPMISGIEEIREAKRIFNDVMRDLIEEDIPVGRDIEIGVMIEVPSAGIVADELAKEVDFFSIGTNDLIQYVLAIDRINE
RVGYLYEPLHPAVLRLIQQIVSAGHRNGIRVEMCGEMAGEPLYALILLGLELDELSMNPLAIPRMKKIIQNSTLKESREL
LDKVLILHSATEIRDYVEHYMRQRFPDEFPENGP
>Mature_594_residues
MNTTDEIKKTFVLKGVGVSPGIASGKAFLFDPLGSEVSFYKLSDKSLITKEIRRFKDALKESERQLLEIKNGIHGVIGPE
PLYIIDVHIMLLRDKAFIRHTVEHIRQSCVNAEWAVQMTTEKYRELFDRVEDEYIRSRFSDIQYVGKMILRNLAGKKRGP
FSEISEEVIVVSSDLSPADTAQMMVHKVLGFATDMGGKTSHTAIVARSIAIPAVVGLKNITRVVKTNDDLIIDGASGLII
VNPDPEVARRYQEKKRQYETIQEDFLKYAHLPAMTKDHYRVDIGSNIEFIEEIPSAVLHGAEGIGLYRTEFIYINRDQLP
TEEEHFAHYHQVVGVEGLRWSTIRTFDLGGDKFTSDPKLAKEMNPQLGLRAIRFCLKEVEQFKVQLRAIIRASAYGKTRI
MFPMISGIEEIREAKRIFNDVMRDLIEEDIPVGRDIEIGVMIEVPSAGIVADELAKEVDFFSIGTNDLIQYVLAIDRINE
RVGYLYEPLHPAVLRLIQQIVSAGHRNGIRVEMCGEMAGEPLYALILLGLELDELSMNPLAIPRMKKIIQNSTLKESREL
LDKVLILHSATEIRDYVEHYMRQRFPDEFPENGP

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=573, Percent_Identity=41.1867364746946, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI48994992, Length=501, Percent_Identity=35.1297405189621, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789193, Length=581, Percent_Identity=33.2185886402754, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788726, Length=492, Percent_Identity=34.349593495935, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1787994, Length=418, Percent_Identity=24.8803827751196, Blast_Score=92, Evalue=9e-20,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 67484; Mature: 67484

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTDEIKKTFVLKGVGVSPGIASGKAFLFDPLGSEVSFYKLSDKSLITKEIRRFKDALK
CCCHHHHHHHHHEECCCCCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHH
ESERQLLEIKNGIHGVIGPEPLYIIDVHIMLLRDKAFIRHTVEHIRQSCVNAEWAVQMTT
HHHHHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEHH
EKYRELFDRVEDEYIRSRFSDIQYVGKMILRNLAGKKRGPFSEISEEVIVVSSDLSPADT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCHHH
AQMMVHKVLGFATDMGGKTSHTAIVARSIAIPAVVGLKNITRVVKTNDDLIIDGASGLII
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEE
VNPDPEVARRYQEKKRQYETIQEDFLKYAHLPAMTKDHYRVDIGSNIEFIEEIPSAVLHG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCC
AEGIGLYRTEFIYINRDQLPTEEEHFAHYHQVVGVEGLRWSTIRTFDLGGDKFTSDPKLA
CCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEECEEEEEECCCCCCCCCCHHH
KEMNPQLGLRAIRFCLKEVEQFKVQLRAIIRASAYGKTRIMFPMISGIEEIREAKRIFND
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
VMRDLIEEDIPVGRDIEIGVMIEVPSAGIVADELAKEVDFFSIGTNDLIQYVLAIDRINE
HHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHCCEEECCHHHHHHHHHHHHHHHH
RVGYLYEPLHPAVLRLIQQIVSAGHRNGIRVEMCGEMAGEPLYALILLGLELDELSMNPL
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHHHHHHHCCCHHHCCCCCC
AIPRMKKIIQNSTLKESRELLDKVLILHSATEIRDYVEHYMRQRFPDEFPENGP
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNTTDEIKKTFVLKGVGVSPGIASGKAFLFDPLGSEVSFYKLSDKSLITKEIRRFKDALK
CCCHHHHHHHHHEECCCCCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHH
ESERQLLEIKNGIHGVIGPEPLYIIDVHIMLLRDKAFIRHTVEHIRQSCVNAEWAVQMTT
HHHHHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEHH
EKYRELFDRVEDEYIRSRFSDIQYVGKMILRNLAGKKRGPFSEISEEVIVVSSDLSPADT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCHHH
AQMMVHKVLGFATDMGGKTSHTAIVARSIAIPAVVGLKNITRVVKTNDDLIIDGASGLII
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEE
VNPDPEVARRYQEKKRQYETIQEDFLKYAHLPAMTKDHYRVDIGSNIEFIEEIPSAVLHG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCC
AEGIGLYRTEFIYINRDQLPTEEEHFAHYHQVVGVEGLRWSTIRTFDLGGDKFTSDPKLA
CCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEECEEEEEECCCCCCCCCCHHH
KEMNPQLGLRAIRFCLKEVEQFKVQLRAIIRASAYGKTRIMFPMISGIEEIREAKRIFND
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
VMRDLIEEDIPVGRDIEIGVMIEVPSAGIVADELAKEVDFFSIGTNDLIQYVLAIDRINE
HHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHCCEEECCHHHHHHHHHHHHHHHH
RVGYLYEPLHPAVLRLIQQIVSAGHRNGIRVEMCGEMAGEPLYALILLGLELDELSMNPL
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHHHHHHHCCCHHHCCCCCC
AIPRMKKIIQNSTLKESRELLDKVLILHSATEIRDYVEHYMRQRFPDEFPENGP
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]