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The map label for this gene is mrdA [H]

Identifier: 85858470

GI number: 85858470

Start: 625641

End: 627533

Strand: Direct

Name: mrdA [H]

Synonym: SYN_00551

Alternate gene names: 85858470

Gene position: 625641-627533 (Clockwise)

Preceding gene: 85858469

Following gene: 85858471

Centisome position: 19.68

GC content: 50.82

Gene sequence:

>1893_bases
ATGTTGGGGCTGAAAGGTCGGATAAAAGGGCACGAAACCGCTGATTACCGGCAGCGGTTCAAGATTGCTTTTGCAGTCGT
TCTGGTTGCTCTGTCCCTGCTTCTTGTGAGACTGTGGTACCTTCAGGTGATCAAGGGACCTGAGCTCCGGCAGCGTTCTG
AAAACAACAGTGTTCGTCTCCGGAAGATCAATTCTTTACGGGGTATGATTCTGGACACCTCCGGGAATATCCTTGTGGAC
AACCAGACCTCCTTTGATCTGATTTTTATTCCCAATCGTCCGGAAGATAAAAAATATGCGGGTCCGGCATTGTCCGCCCT
TTATGCGTCAAGATCCTGGGAGCTGCCGGAAGGGCTATCCATGATAGAAAAGGCCCATCCTTTCATCCCTTCGAAAATTG
ATAAAAATATCAGTCGGGAAAAACTGGCGATGATAGAAACCCACACTCTTGAACTACCAGGTGTGGCGGTTGAGGTTGTA
CCGATTCGTAAATATCTGGCCGGTGAGATGATTGCCCATATTGTGGGATATGTCAATGAGGTCAGTCAGGAAGAACTGGA
AAAATCAAACGGAATGTACAGCCCCGGCGATATGGTTGGCAAGTACGGCATTGAAAAATATTACGATGCCTATCTCCGGG
GAAAAAATGGGGCAGAGCAGGTTGAAGTGAACGCTCTTGGAAAAGCTGTCCGGGTTCTCGGAAAAATTGATCCCACTCCA
GGTTATAATCTTGTTTTGACGATTGACGCCAACCTCCAGAAGGTTGCCTGGGAGGCCATGAAAGGTAAATCGGGTGCGGT
AGTGGCCATGGACCCCCGGGACGGTTCCATTTACGCAATGGTCAGCTCCCCCGGATTTGATCCCAATCTGTTTAATGGGG
GGATTTCAACAGCCAATTGGAACGCTCTTGCTCGAGATCCGAATCACCCGATGGAACAGCGGGCGATTTCAGGACAGTAT
CCGCCCGGTTCGACTTACAAATTAATGGTGGCCGCCGCGGCTCTGGAAGAGGGGCTGATATCGCCTTCTACATCTTTTCT
GTGCACCGGTTCTTTTGAACTGGGAAACCGGTCTTATCGCTGCTGGCAGAAACATGGACATGGTATGGTCAATCTCCATC
GGGCTATCGTTGAATCCTGCGATGTTTATTTTTACAGGATCGGGAAAATGCTCGGAGTGGATACAATTGCCTGGTATGCG
AAAAAATTCGGTTTCGGCGCCTTGACAGGTGTTGATCTGCCCCGGGAAAAGGCAGGGATGATCCCCACCAAAGAATGGAA
AATGACAAAATTCAAAAAACCCTGGATGATGGGAGAAACCATCTCAGCGTCGATCGGCCAGGGGTTCAATACGGTCACTC
CCCTGCAATTGGCCAGCGCATACAGTGCCATGGCCAATGGTGGAACCCTCTATCGTCCACACATTGTCAAGAGAATCGAA
TCGCCCGATGGTCGGATAGTAAAGGTAATGAAACCGGAAAAGAAAGGCTCTCTGCCGGTCAGCCTGCAAACCCTGGAGTC
GCTTAAAGGTGGATTATGGGGGGTTGTCAATGAGAAAAACGGCACCGGAGGTGCTTTACGGCGTCCGGAGCAGGATGTCT
GCGGCAAGACGGGGACTTCCCAGGTCATCGGTCTGCCTGCCGGCGAGAAATCGCGGCGTTCCCTGCAATCGATCAGAAAG
TATCAGGATCATGCCCTGTTTACCTGTTTTGCTCCATGCCGGAATCCGGAGATCGTCGTGGCGGTGATTGTCGAACATGG
CGGTCATGGAGGTTCGGCAGCGGCGCCCGTTGCCCGGAAGATATTGGATGCTTACTATGACAGCAAGAAATATGGCGGCA
GCATTCCTGAAAAGAAAACGGACAATGAGGGGCATCCTGCAAAACAACCGTAA

Upstream 100 bases:

>100_bases
TTTCATAAATTTATCAGCGTTTTTCTTCCGCAGGTGTTGGTGGTCAGCCTTATAAGTCCGGCATGTTTTAACACCTTCAG
ACGCTTTGGAGACTTATTGA

Downstream 100 bases:

>100_bases
AAAGGGATGAGGGCAGGATTGAAAAAGATGAAATCGCGAAATAATAAAAATATCGGCAAATTGAATTTTTCAGTGGGCGC
GGCATGAGGTTTGACCGTCG

Product: cell elongation specific D,D-transpeptidase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MLGLKGRIKGHETADYRQRFKIAFAVVLVALSLLLVRLWYLQVIKGPELRQRSENNSVRLRKINSLRGMILDTSGNILVD
NQTSFDLIFIPNRPEDKKYAGPALSALYASRSWELPEGLSMIEKAHPFIPSKIDKNISREKLAMIETHTLELPGVAVEVV
PIRKYLAGEMIAHIVGYVNEVSQEELEKSNGMYSPGDMVGKYGIEKYYDAYLRGKNGAEQVEVNALGKAVRVLGKIDPTP
GYNLVLTIDANLQKVAWEAMKGKSGAVVAMDPRDGSIYAMVSSPGFDPNLFNGGISTANWNALARDPNHPMEQRAISGQY
PPGSTYKLMVAAAALEEGLISPSTSFLCTGSFELGNRSYRCWQKHGHGMVNLHRAIVESCDVYFYRIGKMLGVDTIAWYA
KKFGFGALTGVDLPREKAGMIPTKEWKMTKFKKPWMMGETISASIGQGFNTVTPLQLASAYSAMANGGTLYRPHIVKRIE
SPDGRIVKVMKPEKKGSLPVSLQTLESLKGGLWGVVNEKNGTGGALRRPEQDVCGKTGTSQVIGLPAGEKSRRSLQSIRK
YQDHALFTCFAPCRNPEIVVAVIVEHGGHGGSAAAPVARKILDAYYDSKKYGGSIPEKKTDNEGHPAKQP

Sequences:

>Translated_630_residues
MLGLKGRIKGHETADYRQRFKIAFAVVLVALSLLLVRLWYLQVIKGPELRQRSENNSVRLRKINSLRGMILDTSGNILVD
NQTSFDLIFIPNRPEDKKYAGPALSALYASRSWELPEGLSMIEKAHPFIPSKIDKNISREKLAMIETHTLELPGVAVEVV
PIRKYLAGEMIAHIVGYVNEVSQEELEKSNGMYSPGDMVGKYGIEKYYDAYLRGKNGAEQVEVNALGKAVRVLGKIDPTP
GYNLVLTIDANLQKVAWEAMKGKSGAVVAMDPRDGSIYAMVSSPGFDPNLFNGGISTANWNALARDPNHPMEQRAISGQY
PPGSTYKLMVAAAALEEGLISPSTSFLCTGSFELGNRSYRCWQKHGHGMVNLHRAIVESCDVYFYRIGKMLGVDTIAWYA
KKFGFGALTGVDLPREKAGMIPTKEWKMTKFKKPWMMGETISASIGQGFNTVTPLQLASAYSAMANGGTLYRPHIVKRIE
SPDGRIVKVMKPEKKGSLPVSLQTLESLKGGLWGVVNEKNGTGGALRRPEQDVCGKTGTSQVIGLPAGEKSRRSLQSIRK
YQDHALFTCFAPCRNPEIVVAVIVEHGGHGGSAAAPVARKILDAYYDSKKYGGSIPEKKTDNEGHPAKQP
>Mature_630_residues
MLGLKGRIKGHETADYRQRFKIAFAVVLVALSLLLVRLWYLQVIKGPELRQRSENNSVRLRKINSLRGMILDTSGNILVD
NQTSFDLIFIPNRPEDKKYAGPALSALYASRSWELPEGLSMIEKAHPFIPSKIDKNISREKLAMIETHTLELPGVAVEVV
PIRKYLAGEMIAHIVGYVNEVSQEELEKSNGMYSPGDMVGKYGIEKYYDAYLRGKNGAEQVEVNALGKAVRVLGKIDPTP
GYNLVLTIDANLQKVAWEAMKGKSGAVVAMDPRDGSIYAMVSSPGFDPNLFNGGISTANWNALARDPNHPMEQRAISGQY
PPGSTYKLMVAAAALEEGLISPSTSFLCTGSFELGNRSYRCWQKHGHGMVNLHRAIVESCDVYFYRIGKMLGVDTIAWYA
KKFGFGALTGVDLPREKAGMIPTKEWKMTKFKKPWMMGETISASIGQGFNTVTPLQLASAYSAMANGGTLYRPHIVKRIE
SPDGRIVKVMKPEKKGSLPVSLQTLESLKGGLWGVVNEKNGTGGALRRPEQDVCGKTGTSQVIGLPAGEKSRRSLQSIRK
YQDHALFTCFAPCRNPEIVVAVIVEHGGHGGSAAAPVARKILDAYYDSKKYGGSIPEKKTDNEGHPAKQP

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=600, Percent_Identity=34.6666666666667, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1786272, Length=602, Percent_Identity=26.4119601328904, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 69119; Mature: 69119

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLKGRIKGHETADYRQRFKIAFAVVLVALSLLLVRLWYLQVIKGPELRQRSENNSVRL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEE
RKINSLRGMILDTSGNILVDNQTSFDLIFIPNRPEDKKYAGPALSALYASRSWELPEGLS
EEECCCCCEEEECCCCEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCHHHH
MIEKAHPFIPSKIDKNISREKLAMIETHTLELPGVAVEVVPIRKYLAGEMIAHIVGYVNE
HHHHHCCCCCCHHHCCCCHHHHHHEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHH
VSQEELEKSNGMYSPGDMVGKYGIEKYYDAYLRGKNGAEQVEVNALGKAVRVLGKIDPTP
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCC
GYNLVLTIDANLQKVAWEAMKGKSGAVVAMDPRDGSIYAMVSSPGFDPNLFNGGISTANW
CCEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCC
NALARDPNHPMEQRAISGQYPPGSTYKLMVAAAALEEGLISPSTSFLCTGSFELGNRSYR
CEECCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHCCCCCHH
CWQKHGHGMVNLHRAIVESCDVYFYRIGKMLGVDTIAWYAKKFGFGALTGVDLPREKAGM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHCCC
IPTKEWKMTKFKKPWMMGETISASIGQGFNTVTPLQLASAYSAMANGGTLYRPHIVKRIE
CCCCCCCHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEECCHHHHHHC
SPDGRIVKVMKPEKKGSLPVSLQTLESLKGGLWGVVNEKNGTGGALRRPEQDVCGKTGTS
CCCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHCCCCCCC
QVIGLPAGEKSRRSLQSIRKYQDHALFTCFAPCRNPEIVVAVIVEHGGHGGSAAAPVARK
CEEECCCCCHHHHHHHHHHHHHHCEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHH
ILDAYYDSKKYGGSIPEKKTDNEGHPAKQP
HHHHHHCCHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLGLKGRIKGHETADYRQRFKIAFAVVLVALSLLLVRLWYLQVIKGPELRQRSENNSVRL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEE
RKINSLRGMILDTSGNILVDNQTSFDLIFIPNRPEDKKYAGPALSALYASRSWELPEGLS
EEECCCCCEEEECCCCEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCHHHH
MIEKAHPFIPSKIDKNISREKLAMIETHTLELPGVAVEVVPIRKYLAGEMIAHIVGYVNE
HHHHHCCCCCCHHHCCCCHHHHHHEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHH
VSQEELEKSNGMYSPGDMVGKYGIEKYYDAYLRGKNGAEQVEVNALGKAVRVLGKIDPTP
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCC
GYNLVLTIDANLQKVAWEAMKGKSGAVVAMDPRDGSIYAMVSSPGFDPNLFNGGISTANW
CCEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCC
NALARDPNHPMEQRAISGQYPPGSTYKLMVAAAALEEGLISPSTSFLCTGSFELGNRSYR
CEECCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHCCCCCHH
CWQKHGHGMVNLHRAIVESCDVYFYRIGKMLGVDTIAWYAKKFGFGALTGVDLPREKAGM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHCCC
IPTKEWKMTKFKKPWMMGETISASIGQGFNTVTPLQLASAYSAMANGGTLYRPHIVKRIE
CCCCCCCHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEECCHHHHHHC
SPDGRIVKVMKPEKKGSLPVSLQTLESLKGGLWGVVNEKNGTGGALRRPEQDVCGKTGTS
CCCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHCCCCCCC
QVIGLPAGEKSRRSLQSIRKYQDHALFTCFAPCRNPEIVVAVIVEHGGHGGSAAAPVARK
CEEECCCCCHHHHHHHHHHHHHHCEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHH
ILDAYYDSKKYGGSIPEKKTDNEGHPAKQP
HHHHHHCCHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]