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The map label for this gene is pilC [H]

Identifier: 85858125

GI number: 85858125

Start: 292375

End: 293586

Strand: Direct

Name: pilC [H]

Synonym: SYN_01284

Alternate gene names: 85858125

Gene position: 292375-293586 (Clockwise)

Preceding gene: 85858124

Following gene: 85858127

Centisome position: 9.2

GC content: 50.91

Gene sequence:

>1212_bases
ATGCCGGAGTTCCTGTGGGAAGCAACCACCCGGAGGGGTGAAGTCAAAAAGGGGGAAATGAAGGTTGCGGATGAAGCCGC
TTTACGAACGCTGCTTCGCCGGCAGGGTTTAAAATCGATCACGATCAGGAAAAAACCCAAGGATCTCCTCGAATATCTAC
CCTTCCTCAAACAGAAGGTCAAAGAGAAGCAGATCGTCGTCTTCGCCCGAATCTTTTCGACGATGATCAATTCCGGCCTC
CCGTTGATTCAGTGTCTCGATCTCCTGGCCCAGCAGGAAACCAACAAAACCTTTGCCAAGGCCATCACGGCCATAAAAGA
AGATATTGAAGGCGGTTCATCTTTGACCGATGCCTTGAAAAAGTATCCGGAAATTTTCGATGAACTGTTTGTAAATCTTA
TTGCGGCGGGGGAGAGTGGCGGCATTCTGGACGTCATCCTGGGACGTCTTTCCACGTACATGGAAAAAGCCATGAAACTC
AAACGCAAGGTAAAGGGAGCCCTGACCTACCCGATCAGCGTTCTGGTGATTTCCATCGGTGTGGTGACTCTGCTGCTGTT
GAAGGTCATTCCCGTATTCCAGAAGCTGTTTGCCGACATGGGAGGGGCGTTGCCGGCGCCGACTCAGTTTCTGGTCAATG
CCAGCGCGTTCATGCAGAACTATTTTGTCTATATGGTTGCCGGCGTCATTGCCGCTTTCTTTGCGTTCAAACAATTTTAC
CGCACCGAAAAGGGAACTGTTCTGGTGGACAGTATGCTGCTGAAAATGCCTGTCTTCGGTCCGCTCCTTAAAAAGGTAGC
AGTAGCCAAATTTACCCGAACCCTTTCCACGATGATGAGCAGTGGTGTCCCGATCCTCGATGGTCTTGCCATTGTGAGCA
AGACATCAGGAAACAGGATTGTGGAAGAGGCTCTGATGAATACCAGACGGAGCATCAGTGAAGGAAAAACAATCGCGGAA
CCGCTTGAAGCATCGGGACTTTTTCCTCCCATGGTCGTTCAGATGATTGCCGTGGGCGAACATACCGGCGCCCTGGATGC
CATGCTGGCTAAAATAGCGGATTTTTATGATGATGAAGTGGATGCCGCGGTAAGCGCCATGACCGCTCTGCTGGAACCTT
TCATGATGGTGTTTCTGGGAGGCGTTGTCGGCGGAATGATTATTGCGATGTACCTGCCAATCTTCCAGATGGCTTCCGTG
GTGGGGGGATAA

Upstream 100 bases:

>100_bases
TGCCGTTGTCTTGAGCGAAGGGGCACTTTCCCGGGATGCCTGGATCCAGGGAAGGGCAGAGGTTTTTGCCGGCCAGTCAT
GACCGGAAGGAGTTGTTGCC

Downstream 100 bases:

>100_bases
AAATGGTCGGGATGGCGCGATTTGAACGCGCGACTCCTGCGTCCCGAACGCAGTGCCCTACCAAGCTGGGCCACATCCCG
ACAGGGGGAGTTCATATAAA

Product: general secretion pathway protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MPEFLWEATTRRGEVKKGEMKVADEAALRTLLRRQGLKSITIRKKPKDLLEYLPFLKQKVKEKQIVVFARIFSTMINSGL
PLIQCLDLLAQQETNKTFAKAITAIKEDIEGGSSLTDALKKYPEIFDELFVNLIAAGESGGILDVILGRLSTYMEKAMKL
KRKVKGALTYPISVLVISIGVVTLLLLKVIPVFQKLFADMGGALPAPTQFLVNASAFMQNYFVYMVAGVIAAFFAFKQFY
RTEKGTVLVDSMLLKMPVFGPLLKKVAVAKFTRTLSTMMSSGVPILDGLAIVSKTSGNRIVEEALMNTRRSISEGKTIAE
PLEASGLFPPMVVQMIAVGEHTGALDAMLAKIADFYDDEVDAAVSAMTALLEPFMMVFLGGVVGGMIIAMYLPIFQMASV
VGG

Sequences:

>Translated_403_residues
MPEFLWEATTRRGEVKKGEMKVADEAALRTLLRRQGLKSITIRKKPKDLLEYLPFLKQKVKEKQIVVFARIFSTMINSGL
PLIQCLDLLAQQETNKTFAKAITAIKEDIEGGSSLTDALKKYPEIFDELFVNLIAAGESGGILDVILGRLSTYMEKAMKL
KRKVKGALTYPISVLVISIGVVTLLLLKVIPVFQKLFADMGGALPAPTQFLVNASAFMQNYFVYMVAGVIAAFFAFKQFY
RTEKGTVLVDSMLLKMPVFGPLLKKVAVAKFTRTLSTMMSSGVPILDGLAIVSKTSGNRIVEEALMNTRRSISEGKTIAE
PLEASGLFPPMVVQMIAVGEHTGALDAMLAKIADFYDDEVDAAVSAMTALLEPFMMVFLGGVVGGMIIAMYLPIFQMASV
VGG
>Mature_402_residues
PEFLWEATTRRGEVKKGEMKVADEAALRTLLRRQGLKSITIRKKPKDLLEYLPFLKQKVKEKQIVVFARIFSTMINSGLP
LIQCLDLLAQQETNKTFAKAITAIKEDIEGGSSLTDALKKYPEIFDELFVNLIAAGESGGILDVILGRLSTYMEKAMKLK
RKVKGALTYPISVLVISIGVVTLLLLKVIPVFQKLFADMGGALPAPTQFLVNASAFMQNYFVYMVAGVIAAFFAFKQFYR
TEKGTVLVDSMLLKMPVFGPLLKKVAVAKFTRTLSTMMSSGVPILDGLAIVSKTSGNRIVEEALMNTRRSISEGKTIAEP
LEASGLFPPMVVQMIAVGEHTGALDAMLAKIADFYDDEVDAAVSAMTALLEPFMMVFLGGVVGGMIIAMYLPIFQMASVV
GG

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=400, Percent_Identity=28, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786295, Length=401, Percent_Identity=26.9326683291771, Blast_Score=155, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44034; Mature: 43903

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEFLWEATTRRGEVKKGEMKVADEAALRTLLRRQGLKSITIRKKPKDLLEYLPFLKQKV
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KEKQIVVFARIFSTMINSGLPLIQCLDLLAQQETNKTFAKAITAIKEDIEGGSSLTDALK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KYPEIFDELFVNLIAAGESGGILDVILGRLSTYMEKAMKLKRKVKGALTYPISVLVISIG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVTLLLLKVIPVFQKLFADMGGALPAPTQFLVNASAFMQNYFVYMVAGVIAAFFAFKQFY
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTEKGTVLVDSMLLKMPVFGPLLKKVAVAKFTRTLSTMMSSGVPILDGLAIVSKTSGNRI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
VEEALMNTRRSISEGKTIAEPLEASGLFPPMVVQMIAVGEHTGALDAMLAKIADFYDDEV
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHH
DAAVSAMTALLEPFMMVFLGGVVGGMIIAMYLPIFQMASVVGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PEFLWEATTRRGEVKKGEMKVADEAALRTLLRRQGLKSITIRKKPKDLLEYLPFLKQKV
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]