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The map label for this gene is pyrF

Identifier: 85858123

GI number: 85858123

Start: 290582

End: 291337

Strand: Direct

Name: pyrF

Synonym: SYN_01281

Alternate gene names: 85858123

Gene position: 290582-291337 (Clockwise)

Preceding gene: 85858122

Following gene: 85858124

Centisome position: 9.14

GC content: 58.47

Gene sequence:

>756_bases
GTGACGAAGACTATGTCGAATAAGGAGTCATTGGAGAAGCTTATTTTCGCCCTGGATATGGGGGGCGGACTGGATGATGT
CATGTCCTGGGTGCGGCGTCTTGCGGGCCATGTCGGGGTATTCAAGGTCGGCAAGGAGGCCTTTACCCGTTTCGGCCCAC
AACTTGTTCAGAGTATTCAGGAAACAGGATCGCGGGTTTTCCTGGATCTCAAGTTTCATGATATCCCCAATACGGTAGCC
AGGGCCGCGGAAGGGGCAGTGGAGCTGGGGGTCGCCATGTTCAACGTCCATGCTCTGGGCGGGATGAGCATGATGACCGA
GACTGTCGAATCCGTGAAGAAGATGGCTGGGCGCCGGGAACTGCCCATGCCCCTGATTCTCGGGGTTACGGTTTTGACCA
GCCTCAATGATGAGGATCTTCAGCGGCTTGGTTTTACCTGTACAACGGGAGAGCTGGTCCTGAGACTGGCGCGCATGGCG
CAGGATGCCGGCCTGTCCGGCGTTGTGGCTTCGGCGCAGGATGTTGAAGCCATCAGAGCGGCCTGCGGCAAAGACTTTGT
GATCGTAACCCCCGGAATCCGTGGACTGGCCCGGGTTGCCGGTGATGATCAGAAACGCGTCCTCACGGCTGAAGAAGCTG
TCCGGCGGGGATCGGATTATCTGGTGATCGGCAGGCCCATCCGTACAGCGGAAGACCCTGTGGCTGCGGCGGATGACTTC
TGCCGGGAAATCGCACGGGGGCTGAGTGCAAGATAA

Upstream 100 bases:

>100_bases
GAAATTGCGGATAACAAGGTTTATTTCTCTCATCCGGAATATCTGATGGGGGCCAAGGAAGATTTCAAGCTCATCGCGGA
CGATACGACGGAATTTATCG

Downstream 100 bases:

>100_bases
CCCCATGATACCTGAGCGACAGAGACGGCCATGGAGGTGATTTACGGCGTCAATCCTTTAAGAGAAGCGCTGATCAGCGG
TGGAGAAGGAGTGACGAGAA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTKTMSNKESLEKLIFALDMGGGLDDVMSWVRRLAGHVGVFKVGKEAFTRFGPQLVQSIQETGSRVFLDLKFHDIPNTVA
RAAEGAVELGVAMFNVHALGGMSMMTETVESVKKMAGRRELPMPLILGVTVLTSLNDEDLQRLGFTCTTGELVLRLARMA
QDAGLSGVVASAQDVEAIRAACGKDFVIVTPGIRGLARVAGDDQKRVLTAEEAVRRGSDYLVIGRPIRTAEDPVAAADDF
CREIARGLSAR

Sequences:

>Translated_251_residues
MTKTMSNKESLEKLIFALDMGGGLDDVMSWVRRLAGHVGVFKVGKEAFTRFGPQLVQSIQETGSRVFLDLKFHDIPNTVA
RAAEGAVELGVAMFNVHALGGMSMMTETVESVKKMAGRRELPMPLILGVTVLTSLNDEDLQRLGFTCTTGELVLRLARMA
QDAGLSGVVASAQDVEAIRAACGKDFVIVTPGIRGLARVAGDDQKRVLTAEEAVRRGSDYLVIGRPIRTAEDPVAAADDF
CREIARGLSAR
>Mature_250_residues
TKTMSNKESLEKLIFALDMGGGLDDVMSWVRRLAGHVGVFKVGKEAFTRFGPQLVQSIQETGSRVFLDLKFHDIPNTVAR
AAEGAVELGVAMFNVHALGGMSMMTETVESVKKMAGRRELPMPLILGVTVLTSLNDEDLQRLGFTCTTGELVLRLARMAQ
DAGLSGVVASAQDVEAIRAACGKDFVIVTPGIRGLARVAGDDQKRVLTAEEAVRRGSDYLVIGRPIRTAEDPVAAADDFC
REIARGLSAR

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=220, Percent_Identity=45.4545454545455, Blast_Score=174, Evalue=5e-45,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_SYNAS (Q2LQ82)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_460325.1
- ProteinModelPortal:   Q2LQ82
- STRING:   Q2LQ82
- GeneID:   3884670
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_01281
- NMPDR:   fig|56780.10.peg.279
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q2LQ82
- BioCyc:   SACI56780:SYN_01281-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 27022; Mature: 26890

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 71-71 BINDING 19-19 BINDING 42-42 BINDING 133-133 BINDING 194-194 BINDING 204-204 BINDING 224-224 BINDING 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTMSNKESLEKLIFALDMGGGLDDVMSWVRRLAGHVGVFKVGKEAFTRFGPQLVQSIQ
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHH
ETGSRVFLDLKFHDIPNTVARAAEGAVELGVAMFNVHALGGMSMMTETVESVKKMAGRRE
HCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
LPMPLILGVTVLTSLNDEDLQRLGFTCTTGELVLRLARMAQDAGLSGVVASAQDVEAIRA
CCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ACGKDFVIVTPGIRGLARVAGDDQKRVLTAEEAVRRGSDYLVIGRPIRTAEDPVAAADDF
HHCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHH
CREIARGLSAR
HHHHHHHCCCC
>Mature Secondary Structure 
TKTMSNKESLEKLIFALDMGGGLDDVMSWVRRLAGHVGVFKVGKEAFTRFGPQLVQSIQ
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHH
ETGSRVFLDLKFHDIPNTVARAAEGAVELGVAMFNVHALGGMSMMTETVESVKKMAGRRE
HCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
LPMPLILGVTVLTSLNDEDLQRLGFTCTTGELVLRLARMAQDAGLSGVVASAQDVEAIRA
CCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ACGKDFVIVTPGIRGLARVAGDDQKRVLTAEEAVRRGSDYLVIGRPIRTAEDPVAAADDF
HHCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHH
CREIARGLSAR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA