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The map label for this gene is glnB [H]

Identifier: 85857961

GI number: 85857961

Start: 117366

End: 117752

Strand: Direct

Name: glnB [H]

Synonym: SYN_02212

Alternate gene names: 85857961

Gene position: 117366-117752 (Clockwise)

Preceding gene: 85857960

Following gene: 85857965

Centisome position: 3.69

GC content: 60.21

Gene sequence:

>387_bases
ATGCGAATCGGCCTACACGGTCGTCGATTAGAGAGGCGGAATATTATGAAACTGATTGTTGCCATCATCCAGCCCCACAA
ACTGGGGGAAGTGAAGGAGGCCCTCGAGGCGGTGGACGTGAATCTGATGACCGTTTCCAACGTTCTCGGGCAGGGCCGGC
AGAAAGGGCATACGGAAATCTATCGGGGAACGCGCGAGGCGGGGGGGCTGTTGCGGAAGGTGCGGCTGGACATCGCCGTC
AACGAGGATTTCGTGGAACGGACGATCGAGGCCATTGTCAGGGGCGCCCACACCGGCGAGATCGGCGACGGCAAGATCTT
CGTTCTGGACCTTAAGGAGTGCGTCCGGATCGGTACGGGCGAGCACGGATCCAGGGCCATCGGGTAA

Upstream 100 bases:

>100_bases
CCGGGGTCGTTACCTTCATCCTCTTCAAGGTTGTGGATGCCCTTCTCGGCATGAAAGTGGACAGGAAAAGTGAAATCCTG
GGGCTGGATCTCAGCCAGCA

Downstream 100 bases:

>100_bases
AACCAGGGATTAAATGAATGCAGAGGAATAACCGGCGGCCTTTTTGACAAGTCATCGAGGGACCCTCAGTCCGGCTTCGG
CTGGTCGGATTTCAGGGTTC

Product: nitrogen regulatory protein PII

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MRIGLHGRRLERRNIMKLIVAIIQPHKLGEVKEALEAVDVNLMTVSNVLGQGRQKGHTEIYRGTREAGGLLRKVRLDIAV
NEDFVERTIEAIVRGAHTGEIGDGKIFVLDLKECVRIGTGEHGSRAIG

Sequences:

>Translated_128_residues
MRIGLHGRRLERRNIMKLIVAIIQPHKLGEVKEALEAVDVNLMTVSNVLGQGRQKGHTEIYRGTREAGGLLRKVRLDIAV
NEDFVERTIEAIVRGAHTGEIGDGKIFVLDLKECVRIGTGEHGSRAIG
>Mature_128_residues
MRIGLHGRRLERRNIMKLIVAIIQPHKLGEVKEALEAVDVNLMTVSNVLGQGRQKGHTEIYRGTREAGGLLRKVRLDIAV
NEDFVERTIEAIVRGAHTGEIGDGKIFVLDLKECVRIGTGEHGSRAIG

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=51.7857142857143, Blast_Score=111, Evalue=1e-26,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=45.5357142857143, Blast_Score=107, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 14143; Mature: 14143

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGLHGRRLERRNIMKLIVAIIQPHKLGEVKEALEAVDVNLMTVSNVLGQGRQKGHTEI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHH
YRGTREAGGLLRKVRLDIAVNEDFVERTIEAIVRGAHTGEIGDGKIFVLDLKECVRIGTG
HHCCHHHHHHHHHHHHHHEECHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHCCCC
EHGSRAIG
CCCCCCCC
>Mature Secondary Structure
MRIGLHGRRLERRNIMKLIVAIIQPHKLGEVKEALEAVDVNLMTVSNVLGQGRQKGHTEI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHH
YRGTREAGGLLRKVRLDIAVNEDFVERTIEAIVRGAHTGEIGDGKIFVLDLKECVRIGTG
HHCCHHHHHHHHHHHHHHEECHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHCCCC
EHGSRAIG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA