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The map label for this gene is nrgA [H]

Identifier: 85857960

GI number: 85857960

Start: 116164

End: 117396

Strand: Direct

Name: nrgA [H]

Synonym: SYN_02211

Alternate gene names: 85857960

Gene position: 116164-117396 (Clockwise)

Preceding gene: 85857959

Following gene: 85857961

Centisome position: 3.65

GC content: 59.77

Gene sequence:

>1233_bases
ATGAATGCGGGAGATACAGCTTGGGTGCTTATATCCACCGTACTGGTTTTTGTCATGACGCCCGGCCTGGCTTTTTTCTA
TGGCGGCCTGGTGCGTCGGAAAAATGTTCTGTCCATTCTCATGCAGTGCTTCATCATCATGTGCGTGATCAGTCTGCAGT
GGGTCCTGTACGGATACTCGCTGGCCTTCGGCCCGGATACGGGTTGGGGGCTCATCGGCGGTCTTTCGTGGGTGGGGCTC
TCCGGCGTGGGAGGTTCGCCCAACGGCGATTATGCGTCGACCATTCCCCACCTCGCTTTCATGATGTTTCAGGCGATGTT
CGCCATTATCACCCCCGCGCTGATCATCGGCGCCTTCGCGGAAAGGGTCAGGTTCTCGGCTTTTCTCGTGATGACGGTTC
TCTGGGCCACCCTGGTCTACGATCCGCTGGCTCACTGGGTCTGGGGCAGTGGAGGCTGGATGAGAAACCTGGGAGCGCTC
GATTTCGCGGGAGGAATCGTGGTTCATGTCAGTTCGGGGATTTCCGCCCTGGTCATGGCGATTCTGCTGGGGAAACGGAT
CGGCTATGAAAAAGAGCCTTTCCGGCCGCACAATCTGCCCTTTACGGTCCTGGGAGGCGCGCTGCTGTGGTTCGGCTGGT
TCGGCTTCAATGCGGGAAGCGCTCTCGGGGCGAATGAACTGGCGGCCAATGCCTTTGTGACGACCAATACCGCAACAGCG
GCCGCCGGCCTGACCTGGGCACTGATCGAATGGCGGCTTTCCGGCGCCCCGACCGTCCTGGGGGCCGTGACCGGCGCCGT
GGCCGGTCTGGTAGCCGTCACGCCGGCCTGTGGATTTGTAACCCCGGTCAACGCCATTTTTATCGGGGTGAGCGTCAGTG
TGATCTGCTATGCCGCCATCGCCGTCGTCAAGGGCCGGCTGGGTTATGACGATTCCCTGGATGCTTTCGGTGTTCACGGA
GTCGGCGGGGCCTGGGGAACGATTGCCACCGGACTTCTCGCGGAAAAGGCGGTCAATGCCGCCGGGGCGGACGGCCTGTT
TTTCGGCGGTCTGCATCAGTTTCTTGTTCAGTTGATGCTGGTGGGCGTTACCGTTCTGTATGCCGGGGTCGTTACCTTCA
TCCTCTTCAAGGTTGTGGATGCCCTTCTCGGCATGAAAGTGGACAGGAAAAGTGAAATCCTGGGGCTGGATCTCAGCCAG
CAATGCGAATCGGCCTACACGGTCGTCGATTAG

Upstream 100 bases:

>100_bases
TAAGGCGTCAAGTTTACCGGAAAAGCTTTTCGCCGGCCGATCGTAAGCCGCGGGCTTTTCATGATCGGTCTTCCGGGAAA
TTGTTAAGGAGGAATGTATC

Downstream 100 bases:

>100_bases
AGAGGCGGAATATTATGAAACTGATTGTTGCCATCATCCAGCCCCACAAACTGGGGGAAGTGAAGGAGGCCCTCGAGGCG
GTGGACGTGAATCTGATGAC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNAGDTAWVLISTVLVFVMTPGLAFFYGGLVRRKNVLSILMQCFIIMCVISLQWVLYGYSLAFGPDTGWGLIGGLSWVGL
SGVGGSPNGDYASTIPHLAFMMFQAMFAIITPALIIGAFAERVRFSAFLVMTVLWATLVYDPLAHWVWGSGGWMRNLGAL
DFAGGIVVHVSSGISALVMAILLGKRIGYEKEPFRPHNLPFTVLGGALLWFGWFGFNAGSALGANELAANAFVTTNTATA
AAGLTWALIEWRLSGAPTVLGAVTGAVAGLVAVTPACGFVTPVNAIFIGVSVSVICYAAIAVVKGRLGYDDSLDAFGVHG
VGGAWGTIATGLLAEKAVNAAGADGLFFGGLHQFLVQLMLVGVTVLYAGVVTFILFKVVDALLGMKVDRKSEILGLDLSQ
QCESAYTVVD

Sequences:

>Translated_410_residues
MNAGDTAWVLISTVLVFVMTPGLAFFYGGLVRRKNVLSILMQCFIIMCVISLQWVLYGYSLAFGPDTGWGLIGGLSWVGL
SGVGGSPNGDYASTIPHLAFMMFQAMFAIITPALIIGAFAERVRFSAFLVMTVLWATLVYDPLAHWVWGSGGWMRNLGAL
DFAGGIVVHVSSGISALVMAILLGKRIGYEKEPFRPHNLPFTVLGGALLWFGWFGFNAGSALGANELAANAFVTTNTATA
AAGLTWALIEWRLSGAPTVLGAVTGAVAGLVAVTPACGFVTPVNAIFIGVSVSVICYAAIAVVKGRLGYDDSLDAFGVHG
VGGAWGTIATGLLAEKAVNAAGADGLFFGGLHQFLVQLMLVGVTVLYAGVVTFILFKVVDALLGMKVDRKSEILGLDLSQ
QCESAYTVVD
>Mature_410_residues
MNAGDTAWVLISTVLVFVMTPGLAFFYGGLVRRKNVLSILMQCFIIMCVISLQWVLYGYSLAFGPDTGWGLIGGLSWVGL
SGVGGSPNGDYASTIPHLAFMMFQAMFAIITPALIIGAFAERVRFSAFLVMTVLWATLVYDPLAHWVWGSGGWMRNLGAL
DFAGGIVVHVSSGISALVMAILLGKRIGYEKEPFRPHNLPFTVLGGALLWFGWFGFNAGSALGANELAANAFVTTNTATA
AAGLTWALIEWRLSGAPTVLGAVTGAVAGLVAVTPACGFVTPVNAIFIGVSVSVICYAAIAVVKGRLGYDDSLDAFGVHG
VGGAWGTIATGLLAEKAVNAAGADGLFFGGLHQFLVQLMLVGVTVLYAGVVTFILFKVVDALLGMKVDRKSEILGLDLSQ
QCESAYTVVD

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=408, Percent_Identity=48.0392156862745, Blast_Score=340, Evalue=7e-95,
Organism=Caenorhabditis elegans, GI17550182, Length=445, Percent_Identity=30.1123595505618, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17550180, Length=426, Percent_Identity=30.0469483568075, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI133931100, Length=434, Percent_Identity=27.6497695852535, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564042, Length=417, Percent_Identity=27.3381294964029, Blast_Score=128, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6324187, Length=417, Percent_Identity=38.1294964028777, Blast_Score=249, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6325396, Length=417, Percent_Identity=35.4916067146283, Blast_Score=236, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6321559, Length=414, Percent_Identity=34.0579710144928, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI161078314, Length=413, Percent_Identity=33.6561743341404, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43024; Mature: 43024

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAGDTAWVLISTVLVFVMTPGLAFFYGGLVRRKNVLSILMQCFIIMCVISLQWVLYGYS
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFGPDTGWGLIGGLSWVGLSGVGGSPNGDYASTIPHLAFMMFQAMFAIITPALIIGAFA
EEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERVRFSAFLVMTVLWATLVYDPLAHWVWGSGGWMRNLGALDFAGGIVVHVSSGISALVMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHCCCEEEEECCCHHHHHHH
ILLGKRIGYEKEPFRPHNLPFTVLGGALLWFGWFGFNAGSALGANELAANAFVTTNTATA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEECCCHHH
AAGLTWALIEWRLSGAPTVLGAVTGAVAGLVAVTPACGFVTPVNAIFIGVSVSVICYAAI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVVKGRLGYDDSLDAFGVHGVGGAWGTIATGLLAEKAVNAAGADGLFFGGLHQFLVQLML
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
VGVTVLYAGVVTFILFKVVDALLGMKVDRKSEILGLDLSQQCESAYTVVD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MNAGDTAWVLISTVLVFVMTPGLAFFYGGLVRRKNVLSILMQCFIIMCVISLQWVLYGYS
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFGPDTGWGLIGGLSWVGLSGVGGSPNGDYASTIPHLAFMMFQAMFAIITPALIIGAFA
EEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERVRFSAFLVMTVLWATLVYDPLAHWVWGSGGWMRNLGALDFAGGIVVHVSSGISALVMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHCCCEEEEECCCHHHHHHH
ILLGKRIGYEKEPFRPHNLPFTVLGGALLWFGWFGFNAGSALGANELAANAFVTTNTATA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEECCCHHH
AAGLTWALIEWRLSGAPTVLGAVTGAVAGLVAVTPACGFVTPVNAIFIGVSVSVICYAAI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVVKGRLGYDDSLDAFGVHGVGGAWGTIATGLLAEKAVNAAGADGLFFGGLHQFLVQLML
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
VGVTVLYAGVVTFILFKVVDALLGMKVDRKSEILGLDLSQQCESAYTVVD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]