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The map label for this gene is nuoF2 [H]

Identifier: 85857933

GI number: 85857933

Start: 88072

End: 89985

Strand: Direct

Name: nuoF2 [H]

Synonym: SYN_00152

Alternate gene names: 85857933

Gene position: 88072-89985 (Clockwise)

Preceding gene: 85857932

Following gene: 85857934

Centisome position: 2.77

GC content: 56.17

Gene sequence:

>1914_bases
GTGAGCACGATAAATACATGGGATGATTTGAACAAAGCTGCGAAGCGAGGCCTGGCGCGTCTTCTGCCGGCAAAGGTGAA
GATCGTTGTCGGTATGGCTACGAGCGGTATCGCTTCGGGAGCCCAGGCAGTGTATGACGCCTTGGCAAAAGAAATTGCGA
ATCGCGGTCTGGATATCGTCCTCAGCAAGGCGGGCAGTATGGGGATGGACTGCATCGAACCCATCGTCGACGTCATCGCG
CCGGGCAAGCCGCGCCTCTCCTTCAGCAAGATGACGGCGAAGATGGTCCCCATCCTTATTGACCGGGTGGTCAATGGTGA
TCTGAATGGGATACAGCCGGCATACCGTATGGATGAGATGGAGCTTCTCCTTCAGGGAGAGAAAAAGGCCTATTTCTCTG
GTTCCGTGCCGGTCGATTGCTCCGGAATTCCTCAGATCAACGAGCTGCCCTTCTACCGCAAACAAGTTAAGATTGCCTTG
CGCAACTGCGGTATGATCGATCCTGAAAATATGGATGAATATATCGCCAGGGGTGGTTACACCGCCCTGCATAAAGCCCT
CACGACCATGTCGCCGGAGGATGTTATCCTCGAGGTGAAGACTTCAGGCCTTCGAGGGCGCGGCGGCGGCGGCTTCCCGA
CGGGTACGAAATGGGAAAGCTGCCGGCGGGCTGAGGGGGAAATCAAATACGTCATCTGCAACGGTGATGAAGGAGATCCC
GGGGCTTACATGGATCGGAGCCTTATGGAAGGCGATCCCCACAGCGTTCTTGAGGGGATGATCATCGGAGCTTATGCTAT
AGGCGCCCATGAAGGGTACATTTACGTACGGAACGAGTACCCTCTGGCCGTGGCAAATCTTCAGCATGCCATCGGGCAGG
CAAGAGAAGCCGGCCTTCTGGGGAAAAATATCCTCGGCACGGGTTTTGAGTTCGACATCAAGATCGCCAAAGGTGCGGGC
GCCTTTGTCTGTGGAGAATCGACGGCCCTGATGGCTTCCCTGGAAGGGAAAGCGGGCGAGCCTCGGGCCAAGTACATACA
TACGGTGGAACAGGGGCTTTGGAACCGGCCGTCCAACTTGAATAACGTGGAGACATGGGCGAATGTTCCCGTCATTATCG
CCCGGGGCGGCCAATGGTTCTCCGGCATCGGTGTGAAGCACAGCACGGGGACAAAGGTTTTTTCCCTGGTGGGTAAGATC
AACAACATCGGCCTCGTGGAAGTGCCTATGGGCATACCGCTTAAAGACATCATCTACGAGATCGGCGGCGGCATCCCCGG
CGGCAGGAAGTTCAAGGCCGTGCAGACGGGAGGCCCTTCCGGCGGATGCATCCCGGACACGATGATCGATCTGCCCGTTG
ATTTCGATTCCCTCTGGGAAGCCGGCTCCATGATGGGATCCGGGGGGATGATCGTCATGGACGATAAGACCTGCATGGTT
GACATTGCCCGCTACTTTATCGAATTTCTCGTGTCCGAATCCTGCGGGAAGTGCGTGCCCTGCCGGGAGGGAATCTACCG
GATGAACGAGATTCTCCAGGACATCTGTGCGGGAAAGGGCAGAAGGGGTGACCTGGAGCTTCTGGGACGAATGGCAGAAG
CCATCAAGGACGGGTCCCTTTGTGCTCTCGGTGGCAGCGCCCCGAATCCTGTACTGAGCACAATCCGTTATTTCCGGAGC
GAATATGACGCCCACATCCGGAGCAAGAAGTGCCCCGCCGGTGTTTGCAAGGCCCTCATCCAATATAACATCGACAAGGA
GAAGTGTACCGGCTGCATGGCCTGTGCGAAGAAGTGTCCCGTTGAGGCAATCAGTGGCGAGCGGAAAAAGGCCCACGAGA
TTGACCAGGCGAAATGCATCAAGTGCGGAGTTTGCATGGAAACGTGCAAGTTTGATGCGATAACCTTATCATAA

Upstream 100 bases:

>100_bases
TTGCCCCGGTGGTACGGATTGACGGGAACATTCATCCTTACCTCACCCAGGATGAGATTCCCGGGATTCTGAAAAACTAT
CGCAAAGCAGGGGTGAAAAA

Downstream 100 bases:

>100_bases
AGGTAGGCGAGATGGTGAATTTAGTCATAGATGGGAAGAAGATCGAGGCAGAGACCGGCACAACAATCCTTTCCGCTGCC
CGGGACAACGGGATCGTCAT

Product: NADH:ubiquinone oxidoreductase, NADH-binding subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MSTINTWDDLNKAAKRGLARLLPAKVKIVVGMATSGIASGAQAVYDALAKEIANRGLDIVLSKAGSMGMDCIEPIVDVIA
PGKPRLSFSKMTAKMVPILIDRVVNGDLNGIQPAYRMDEMELLLQGEKKAYFSGSVPVDCSGIPQINELPFYRKQVKIAL
RNCGMIDPENMDEYIARGGYTALHKALTTMSPEDVILEVKTSGLRGRGGGGFPTGTKWESCRRAEGEIKYVICNGDEGDP
GAYMDRSLMEGDPHSVLEGMIIGAYAIGAHEGYIYVRNEYPLAVANLQHAIGQAREAGLLGKNILGTGFEFDIKIAKGAG
AFVCGESTALMASLEGKAGEPRAKYIHTVEQGLWNRPSNLNNVETWANVPVIIARGGQWFSGIGVKHSTGTKVFSLVGKI
NNIGLVEVPMGIPLKDIIYEIGGGIPGGRKFKAVQTGGPSGGCIPDTMIDLPVDFDSLWEAGSMMGSGGMIVMDDKTCMV
DIARYFIEFLVSESCGKCVPCREGIYRMNEILQDICAGKGRRGDLELLGRMAEAIKDGSLCALGGSAPNPVLSTIRYFRS
EYDAHIRSKKCPAGVCKALIQYNIDKEKCTGCMACAKKCPVEAISGERKKAHEIDQAKCIKCGVCMETCKFDAITLS

Sequences:

>Translated_637_residues
MSTINTWDDLNKAAKRGLARLLPAKVKIVVGMATSGIASGAQAVYDALAKEIANRGLDIVLSKAGSMGMDCIEPIVDVIA
PGKPRLSFSKMTAKMVPILIDRVVNGDLNGIQPAYRMDEMELLLQGEKKAYFSGSVPVDCSGIPQINELPFYRKQVKIAL
RNCGMIDPENMDEYIARGGYTALHKALTTMSPEDVILEVKTSGLRGRGGGGFPTGTKWESCRRAEGEIKYVICNGDEGDP
GAYMDRSLMEGDPHSVLEGMIIGAYAIGAHEGYIYVRNEYPLAVANLQHAIGQAREAGLLGKNILGTGFEFDIKIAKGAG
AFVCGESTALMASLEGKAGEPRAKYIHTVEQGLWNRPSNLNNVETWANVPVIIARGGQWFSGIGVKHSTGTKVFSLVGKI
NNIGLVEVPMGIPLKDIIYEIGGGIPGGRKFKAVQTGGPSGGCIPDTMIDLPVDFDSLWEAGSMMGSGGMIVMDDKTCMV
DIARYFIEFLVSESCGKCVPCREGIYRMNEILQDICAGKGRRGDLELLGRMAEAIKDGSLCALGGSAPNPVLSTIRYFRS
EYDAHIRSKKCPAGVCKALIQYNIDKEKCTGCMACAKKCPVEAISGERKKAHEIDQAKCIKCGVCMETCKFDAITLS
>Mature_636_residues
STINTWDDLNKAAKRGLARLLPAKVKIVVGMATSGIASGAQAVYDALAKEIANRGLDIVLSKAGSMGMDCIEPIVDVIAP
GKPRLSFSKMTAKMVPILIDRVVNGDLNGIQPAYRMDEMELLLQGEKKAYFSGSVPVDCSGIPQINELPFYRKQVKIALR
NCGMIDPENMDEYIARGGYTALHKALTTMSPEDVILEVKTSGLRGRGGGGFPTGTKWESCRRAEGEIKYVICNGDEGDPG
AYMDRSLMEGDPHSVLEGMIIGAYAIGAHEGYIYVRNEYPLAVANLQHAIGQAREAGLLGKNILGTGFEFDIKIAKGAGA
FVCGESTALMASLEGKAGEPRAKYIHTVEQGLWNRPSNLNNVETWANVPVIIARGGQWFSGIGVKHSTGTKVFSLVGKIN
NIGLVEVPMGIPLKDIIYEIGGGIPGGRKFKAVQTGGPSGGCIPDTMIDLPVDFDSLWEAGSMMGSGGMIVMDDKTCMVD
IARYFIEFLVSESCGKCVPCREGIYRMNEILQDICAGKGRRGDLELLGRMAEAIKDGSLCALGGSAPNPVLSTIRYFRSE
YDAHIRSKKCPAGVCKALIQYNIDKEKCTGCMACAKKCPVEAISGERKKAHEIDQAKCIKCGVCMETCKFDAITLS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=396, Percent_Identity=41.9191919191919, Blast_Score=318, Evalue=9e-87,
Organism=Homo sapiens, GI20149568, Length=396, Percent_Identity=41.9191919191919, Blast_Score=318, Evalue=1e-86,
Organism=Escherichia coli, GI1788620, Length=424, Percent_Identity=42.2169811320755, Blast_Score=353, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17531727, Length=395, Percent_Identity=41.2658227848101, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI19920794, Length=376, Percent_Identity=42.5531914893617, Blast_Score=308, Evalue=9e-84,
Organism=Drosophila melanogaster, GI45550452, Length=378, Percent_Identity=40.2116402116402, Blast_Score=296, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24653819, Length=385, Percent_Identity=36.1038961038961, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=34.6478873239437, Blast_Score=213, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 68446; Mature: 68315

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTINTWDDLNKAAKRGLARLLPAKVKIVVGMATSGIASGAQAVYDALAKEIANRGLDIV
CCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEE
LSKAGSMGMDCIEPIVDVIAPGKPRLSFSKMTAKMVPILIDRVVNGDLNGIQPAYRMDEM
EECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ELLLQGEKKAYFSGSVPVDCSGIPQINELPFYRKQVKIALRNCGMIDPENMDEYIARGGY
HHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCH
TALHKALTTMSPEDVILEVKTSGLRGRGGGGFPTGTKWESCRRAEGEIKYVICNGDEGDP
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCC
GAYMDRSLMEGDPHSVLEGMIIGAYAIGAHEGYIYVRNEYPLAVANLQHAIGQAREAGLL
CHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
GKNILGTGFEFDIKIAKGAGAFVCGESTALMASLEGKAGEPRAKYIHTVEQGLWNRPSNL
CCCCCCCCCEEEEEEECCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCC
NNVETWANVPVIIARGGQWFSGIGVKHSTGTKVFSLVGKINNIGLVEVPMGIPLKDIIYE
CCCHHHCCCCEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
IGGGIPGGRKFKAVQTGGPSGGCIPDTMIDLPVDFDSLWEAGSMMGSGGMIVMDDKTCMV
HCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCCEEEECCCHHHH
DIARYFIEFLVSESCGKCVPCREGIYRMNEILQDICAGKGRRGDLELLGRMAEAIKDGSL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCE
CALGGSAPNPVLSTIRYFRSEYDAHIRSKKCPAGVCKALIQYNIDKEKCTGCMACAKKCP
EEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
VEAISGERKKAHEIDQAKCIKCGVCMETCKFDAITLS
HHHHCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
STINTWDDLNKAAKRGLARLLPAKVKIVVGMATSGIASGAQAVYDALAKEIANRGLDIV
CCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEE
LSKAGSMGMDCIEPIVDVIAPGKPRLSFSKMTAKMVPILIDRVVNGDLNGIQPAYRMDEM
EECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ELLLQGEKKAYFSGSVPVDCSGIPQINELPFYRKQVKIALRNCGMIDPENMDEYIARGGY
HHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCH
TALHKALTTMSPEDVILEVKTSGLRGRGGGGFPTGTKWESCRRAEGEIKYVICNGDEGDP
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCC
GAYMDRSLMEGDPHSVLEGMIIGAYAIGAHEGYIYVRNEYPLAVANLQHAIGQAREAGLL
CHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
GKNILGTGFEFDIKIAKGAGAFVCGESTALMASLEGKAGEPRAKYIHTVEQGLWNRPSNL
CCCCCCCCCEEEEEEECCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCC
NNVETWANVPVIIARGGQWFSGIGVKHSTGTKVFSLVGKINNIGLVEVPMGIPLKDIIYE
CCCHHHCCCCEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
IGGGIPGGRKFKAVQTGGPSGGCIPDTMIDLPVDFDSLWEAGSMMGSGGMIVMDDKTCMV
HCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCCEEEECCCHHHH
DIARYFIEFLVSESCGKCVPCREGIYRMNEILQDICAGKGRRGDLELLGRMAEAIKDGSL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCE
CALGGSAPNPVLSTIRYFRSEYDAHIRSKKCPAGVCKALIQYNIDKEKCTGCMACAKKCP
EEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
VEAISGERKKAHEIDQAKCIKCGVCMETCKFDAITLS
HHHHCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]