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The map label for this gene is nuoE [H]

Identifier: 85857932

GI number: 85857932

Start: 87554

End: 88075

Strand: Direct

Name: nuoE [H]

Synonym: SYN_00153

Alternate gene names: 85857932

Gene position: 87554-88075 (Clockwise)

Preceding gene: 85857931

Following gene: 85857933

Centisome position: 2.75

GC content: 51.34

Gene sequence:

>522_bases
GTGGCGGGAAATGTAACCGAAAAAATCAAGGGGAAAATAATGAACAAAGAGGTAGACAGGGGGAAAATCAGGGAGATTAT
CAGCAAGTACGATGGAGAAAAAAGTGCTGCCGTCGCCGTATTGCAGGATCTCCAGGAGGAATTCCGATACCTGCCGAAGG
AGGCCTTAACCATCGTGAGTGATGAGCTTCAGGTGCCCTTGAGCAGGATCTATGAGATTGCGACCTTCTACAATGTCTTC
AGCCTGAAGCCGCGGGGTAAATATCTTATTGAAGTGTGCGCCGGTACGGCTTGCCATGTTCAGGGAGGCTTCAACCTCAT
GAACCGGCTGGAGAGGAATCTCAATATCTCCTGCGGAGAGACGACGGAGGATGCCATGTTCACCCTTGAGGAGGTGCGGT
GTCTCGGCTGCTGCAGTCTTGCCCCGGTGGTACGGATTGACGGGAACATTCATCCTTACCTCACCCAGGATGAGATTCCC
GGGATTCTGAAAAACTATCGCAAAGCAGGGGTGAAAAAGTGA

Upstream 100 bases:

>100_bases
GCCGCTTCCGGCATCTGACTCCTGATGAGATCGGAATGTTTCAGAAGAGGGTGAAGCTGGAGTACAATAAACTCATGAAC
AAGATGAAGCATTCTCGCTC

Downstream 100 bases:

>100_bases
GCACGATAAATACATGGGATGATTTGAACAAAGCTGCGAAGCGAGGCCTGGCGCGTCTTCTGCCGGCAAAGGTGAAGATC
GTTGTCGGTATGGCTACGAG

Product: NADH:ubiquinone oxidoreductase 24 kD subunit NuoE

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MAGNVTEKIKGKIMNKEVDRGKIREIISKYDGEKSAAVAVLQDLQEEFRYLPKEALTIVSDELQVPLSRIYEIATFYNVF
SLKPRGKYLIEVCAGTACHVQGGFNLMNRLERNLNISCGETTEDAMFTLEEVRCLGCCSLAPVVRIDGNIHPYLTQDEIP
GILKNYRKAGVKK

Sequences:

>Translated_173_residues
MAGNVTEKIKGKIMNKEVDRGKIREIISKYDGEKSAAVAVLQDLQEEFRYLPKEALTIVSDELQVPLSRIYEIATFYNVF
SLKPRGKYLIEVCAGTACHVQGGFNLMNRLERNLNISCGETTEDAMFTLEEVRCLGCCSLAPVVRIDGNIHPYLTQDEIP
GILKNYRKAGVKK
>Mature_172_residues
AGNVTEKIKGKIMNKEVDRGKIREIISKYDGEKSAAVAVLQDLQEEFRYLPKEALTIVSDELQVPLSRIYEIATFYNVFS
LKPRGKYLIEVCAGTACHVQGGFNLMNRLERNLNISCGETTEDAMFTLEEVRCLGCCSLAPVVRIDGNIHPYLTQDEIPG
ILKNYRKAGVKK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=154, Percent_Identity=40.2597402597403, Blast_Score=116, Evalue=8e-27,
Organism=Escherichia coli, GI1788621, Length=150, Percent_Identity=32.6666666666667, Blast_Score=101, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17561328, Length=171, Percent_Identity=39.766081871345, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI18859877, Length=151, Percent_Identity=38.4105960264901, Blast_Score=113, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21355353, Length=148, Percent_Identity=30.4054054054054, Blast_Score=93, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19418; Mature: 19287

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNVTEKIKGKIMNKEVDRGKIREIISKYDGEKSAAVAVLQDLQEEFRYLPKEALTIVS
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
DELQVPLSRIYEIATFYNVFSLKPRGKYLIEVCAGTACHVQGGFNLMNRLERNLNISCGE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCC
TTEDAMFTLEEVRCLGCCSLAPVVRIDGNIHPYLTQDEIPGILKNYRKAGVKK
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
AGNVTEKIKGKIMNKEVDRGKIREIISKYDGEKSAAVAVLQDLQEEFRYLPKEALTIVS
CCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
DELQVPLSRIYEIATFYNVFSLKPRGKYLIEVCAGTACHVQGGFNLMNRLERNLNISCGE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCC
TTEDAMFTLEEVRCLGCCSLAPVVRIDGNIHPYLTQDEIPGILKNYRKAGVKK
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]