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The map label for this gene is pvuIIM [H]

Identifier: 85857855

GI number: 85857855

Start: 12576

End: 13454

Strand: Direct

Name: pvuIIM [H]

Synonym: SYN_02042

Alternate gene names: 85857855

Gene position: 12576-13454 (Clockwise)

Preceding gene: 85857854

Following gene: 85857856

Centisome position: 0.4

GC content: 39.82

Gene sequence:

>879_bases
ATGGAAGTAACTACAGATATATATCTTGGGGATAGCAGGGAACAACTAAAACTGCTTCCAGATGATTCTGTCGACCTGAT
AGTGACGTCTCCGCCTTACGCAGATCAGCGTAAAAATACATATGGCGGCATCCATCCTGATAAATACGTTAATTGGTTCT
TGCCTATTTCAGTACAGCTTTTACGTGTTCTCAAGCCGACGGGAACCTTTATTTTAAACATCAAAGAAAAAGTAGTCGAG
GGCGAGCGCAGCACTTACGTAATGGAGCTCATATTAGAAATGCGCAAACAGGGATGGCTATGGACGGAAGAGTTTATTTG
GCATAAGAAAAATTGCTACCCAGGAAAATGGCCTAATCGTTTTCGCGATGCCTGGGAGAGACTTCTTCAGTTTAATAAAA
ATCGAAAATTTCATATGTATCAGGAAGAAGTAATGGTTCAAATGGGCGAATGGGCAAACTCAAGACTGAAAAACCTTTCT
GAGACAGACAAAATCCGTGACAACTCAAAAGTAGGAAGCGGATTTGGTAAAAATATTTCAAACTGGCTTGAAAGAGATAA
GGCATATCCCACAAATGTTTTGCACTTGGCTACCGAATGTAACAACAAAAACCATAGCGCGGCTTTTCCTGAAGGGTTGC
CTGAATGGTTTATTAAACTTTTTACAAAAGAAAAAGATACGGTTCTTGATCCCTTTATGGGTTCAGGGACAACACTGATT
GTCGCTAACAGAATGAGGCGAAATTCAATAGGAATCGACATCGTTCCAGAGTATTGTGAAATGGTAAAAAAGCAATTGAA
AGCGGTAGAGTTATATCTATTGGAACCAAAGGCGGAATATGAAAAAACTAGGGTTGAAAAGCGTATCACAATACGTTGA

Upstream 100 bases:

>100_bases
CATAACGAGACGCCAGAGCCGATCGCAGCCCGCGGGACTGCTCCGGCTCAGCTTTTCATTAGACGTATGGTGAAAACACT
TTTATATTAAGCGAGATCAA

Downstream 100 bases:

>100_bases
ACAAAACATAGGGACTTTTCATCAAAAGCGCATTCAAAGCCTTGATGGATTAAAGTTGTCGAATGTTCTTAAACGAAAAA
ATCCATACTTATTTAAAGCA

Product: DNA modification methylase

Products: NA

Alternate protein names: M.PvuII; N-4 cytosine-specific methyltransferase PvuII [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MEVTTDIYLGDSREQLKLLPDDSVDLIVTSPPYADQRKNTYGGIHPDKYVNWFLPISVQLLRVLKPTGTFILNIKEKVVE
GERSTYVMELILEMRKQGWLWTEEFIWHKKNCYPGKWPNRFRDAWERLLQFNKNRKFHMYQEEVMVQMGEWANSRLKNLS
ETDKIRDNSKVGSGFGKNISNWLERDKAYPTNVLHLATECNNKNHSAAFPEGLPEWFIKLFTKEKDTVLDPFMGSGTTLI
VANRMRRNSIGIDIVPEYCEMVKKQLKAVELYLLEPKAEYEKTRVEKRITIR

Sequences:

>Translated_292_residues
MEVTTDIYLGDSREQLKLLPDDSVDLIVTSPPYADQRKNTYGGIHPDKYVNWFLPISVQLLRVLKPTGTFILNIKEKVVE
GERSTYVMELILEMRKQGWLWTEEFIWHKKNCYPGKWPNRFRDAWERLLQFNKNRKFHMYQEEVMVQMGEWANSRLKNLS
ETDKIRDNSKVGSGFGKNISNWLERDKAYPTNVLHLATECNNKNHSAAFPEGLPEWFIKLFTKEKDTVLDPFMGSGTTLI
VANRMRRNSIGIDIVPEYCEMVKKQLKAVELYLLEPKAEYEKTRVEKRITIR
>Mature_292_residues
MEVTTDIYLGDSREQLKLLPDDSVDLIVTSPPYADQRKNTYGGIHPDKYVNWFLPISVQLLRVLKPTGTFILNIKEKVVE
GERSTYVMELILEMRKQGWLWTEEFIWHKKNCYPGKWPNRFRDAWERLLQFNKNRKFHMYQEEVMVQMGEWANSRLKNLS
ETDKIRDNSKVGSGFGKNISNWLERDKAYPTNVLHLATECNNKNHSAAFPEGLPEWFIKLFTKEKDTVLDPFMGSGTTLI
VANRMRRNSIGIDIVPEYCEMVKKQLKAVELYLLEPKAEYEKTRVEKRITIR

Specific function: This methylase recognizes the double-stranded sequence CAGCTG, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the PvuII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 34356; Mature: 34356

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVTTDIYLGDSREQLKLLPDDSVDLIVTSPPYADQRKNTYGGIHPDKYVNWFLPISVQL
CCEEEEEEECCCHHHEEECCCCCCEEEEECCCCCCCCCCCCCCCCCHHHEEEEEHHHHHH
LRVLKPTGTFILNIKEKVVEGERSTYVMELILEMRKQGWLWTEEFIWHKKNCYPGKWPNR
HHHHCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCCCCCCHH
FRDAWERLLQFNKNRKFHMYQEEVMVQMGEWANSRLKNLSETDKIRDNSKVGSGFGKNIS
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHH
NWLERDKAYPTNVLHLATECNNKNHSAAFPEGLPEWFIKLFTKEKDTVLDPFMGSGTTLI
HHHHHHCCCCCHHEEEEHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEE
VANRMRRNSIGIDIVPEYCEMVKKQLKAVELYLLEPKAEYEKTRVEKRITIR
EEECHHHCCCCCEECHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEVTTDIYLGDSREQLKLLPDDSVDLIVTSPPYADQRKNTYGGIHPDKYVNWFLPISVQL
CCEEEEEEECCCHHHEEECCCCCCEEEEECCCCCCCCCCCCCCCCCHHHEEEEEHHHHHH
LRVLKPTGTFILNIKEKVVEGERSTYVMELILEMRKQGWLWTEEFIWHKKNCYPGKWPNR
HHHHCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCCCCCCHH
FRDAWERLLQFNKNRKFHMYQEEVMVQMGEWANSRLKNLSETDKIRDNSKVGSGFGKNIS
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHH
NWLERDKAYPTNVLHLATECNNKNHSAAFPEGLPEWFIKLFTKEKDTVLDPFMGSGTTLI
HHHHHHCCCCCHHEEEEHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEE
VANRMRRNSIGIDIVPEYCEMVKKQLKAVELYLLEPKAEYEKTRVEKRITIR
EEECHHHCCCCCEECHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2662138; 7607491; 9207015 [H]