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The map label for this gene is dnaA [H]

Identifier: 85857846

GI number: 85857846

Start: 155

End: 1528

Strand: Direct

Name: dnaA [H]

Synonym: SYN_02051

Alternate gene names: 85857846

Gene position: 155-1528 (Clockwise)

Preceding gene: NA

Following gene: 85857847

Centisome position: 0.0

GC content: 47.23

Gene sequence:

>1374_bases
GTGGACAGGTATTGTATAGTGGATAATCTCTGGGAAGAAAGTATTAAAATCATTAAGGAAAAAATTAGCCCACAGAATTT
TGAAACCTGGATTCGTCCGCTTAAAGTGGTCTCTCTCGAAGGGAATCATGCCAGCCTGTCGGTACCGAACAAGTTTTTTA
AAGACTGGTTGACTGAAAACTATCGGGATGTCATTTCCGACGCCGTATCCTCATCGGCTGGAAAAGACTATGCGATTGAT
TTTGTTATCCTTCAGGAATCTGAAAAAAATTCCCGCTCCGCTCACCCGGCGAGAAAGAAAAAAATCATCGAGCAGATCCC
CCAAGAAAAAATTCGTCCCAAAATTCATCCCACGTTAAACCCAAATTACAGTTTTGAACGTTTCGTTGTGGGCTCATCCA
ATCAGTTCGCCCATGCGGCAGCGGTTGCCGTTGCCGAACAGCCGGCCAAAAATTACAATCCTCTGTTCATCTACGGAGGC
GTGGGGCTGGGTAAGACGCATCTCCTGAATGCCATCGGACTGCTGAGCATGTCCATCTATCCCGATATGAACGTGGTCTA
CGTCTCCGCGGAAGAGTTCATGAACGAACTGATTCTCTCCATCCGCTACGACAAGATGCCTCAGTTCCGGGAAAAGTTCC
GCAACATCGACTGCCTTTTGATGGATGATATTCAGTTTATTGCCGGAAAGGAGCGAACACAGGAAGAATTCTTTCACACC
TTCAACACGCTTCATGATTCCGGAAAACAGATCGTCGTCACAAGCGACAAATTTCCCAAGGACATCCCGAACCTGGAAGG
CCGCCTCCGTTCCCGTTTTGAGTGGGGTCTGATCGCCGATATCCAGCCGCCGGAAATTGAAACCAAAATTGCCATCCTGG
AAAAAAAGGCCCAGGAAAACAATATTCATATCCCGACCAATGTGGCCTATTACATCTCTTCCCATGCCGAATCCAACATC
CGCGAGCTGGAAGGTTTTCTCGTCCGTATTGCCGCCTATTCCTCGGTTACGGGAAGAAACATCGATCTGGATCTGGTCAA
GGAAGTTCTGAAAGACATCATCAAGCATCGGGAATCCGACGAAATTTCCTTCGAAGAAATTCTCAAGGCGGTCGCTGCCC
GATATAACCTGAAAATCGCTGATATCAAGTCGCAGAGCAAAAACAAGAACATCGCCATGGCGAGGCAGGTAACGATGTAC
ATGGCCCGGAAACTGACCGGAAGCTCTTTTCCGGACATCGGAGAAAAAATCGGGGGAAGGGATCATTCCACAGTTATCTA
CGCCAACAATAAAATCCGTAAACTTCTGGAGACAGATTCCAGGTTGAAAGCCACGCTCCAGGAAATCGAAGATCAACTCC
TCAAGAAAAACTAA

Upstream 100 bases:

>100_bases
CGCCTGAAGAATCCCGATGAAGGCAGCATTTGGTACTTCATCTTTTCAATCAATAGATGGTTAATTTTTTCAATAGTTAT
TAACAATTGTTGATAACCTT

Downstream 100 bases:

>100_bases
CAGAGCAACAAGGGACTTATCAACAGCCTGTTTTTGTGGTAACATATTAAAAATTTAAAGAATTTATGTTTATAAAGACT
TATCCACAGGTCTTATTACT

Product: chromosomal replication initiator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MDRYCIVDNLWEESIKIIKEKISPQNFETWIRPLKVVSLEGNHASLSVPNKFFKDWLTENYRDVISDAVSSSAGKDYAID
FVILQESEKNSRSAHPARKKKIIEQIPQEKIRPKIHPTLNPNYSFERFVVGSSNQFAHAAAVAVAEQPAKNYNPLFIYGG
VGLGKTHLLNAIGLLSMSIYPDMNVVYVSAEEFMNELILSIRYDKMPQFREKFRNIDCLLMDDIQFIAGKERTQEEFFHT
FNTLHDSGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLIADIQPPEIETKIAILEKKAQENNIHIPTNVAYYISSHAESNI
RELEGFLVRIAAYSSVTGRNIDLDLVKEVLKDIIKHRESDEISFEEILKAVAARYNLKIADIKSQSKNKNIAMARQVTMY
MARKLTGSSFPDIGEKIGGRDHSTVIYANNKIRKLLETDSRLKATLQEIEDQLLKKN

Sequences:

>Translated_457_residues
MDRYCIVDNLWEESIKIIKEKISPQNFETWIRPLKVVSLEGNHASLSVPNKFFKDWLTENYRDVISDAVSSSAGKDYAID
FVILQESEKNSRSAHPARKKKIIEQIPQEKIRPKIHPTLNPNYSFERFVVGSSNQFAHAAAVAVAEQPAKNYNPLFIYGG
VGLGKTHLLNAIGLLSMSIYPDMNVVYVSAEEFMNELILSIRYDKMPQFREKFRNIDCLLMDDIQFIAGKERTQEEFFHT
FNTLHDSGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLIADIQPPEIETKIAILEKKAQENNIHIPTNVAYYISSHAESNI
RELEGFLVRIAAYSSVTGRNIDLDLVKEVLKDIIKHRESDEISFEEILKAVAARYNLKIADIKSQSKNKNIAMARQVTMY
MARKLTGSSFPDIGEKIGGRDHSTVIYANNKIRKLLETDSRLKATLQEIEDQLLKKN
>Mature_457_residues
MDRYCIVDNLWEESIKIIKEKISPQNFETWIRPLKVVSLEGNHASLSVPNKFFKDWLTENYRDVISDAVSSSAGKDYAID
FVILQESEKNSRSAHPARKKKIIEQIPQEKIRPKIHPTLNPNYSFERFVVGSSNQFAHAAAVAVAEQPAKNYNPLFIYGG
VGLGKTHLLNAIGLLSMSIYPDMNVVYVSAEEFMNELILSIRYDKMPQFREKFRNIDCLLMDDIQFIAGKERTQEEFFHT
FNTLHDSGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLIADIQPPEIETKIAILEKKAQENNIHIPTNVAYYISSHAESNI
RELEGFLVRIAAYSSVTGRNIDLDLVKEVLKDIIKHRESDEISFEEILKAVAARYNLKIADIKSQSKNKNIAMARQVTMY
MARKLTGSSFPDIGEKIGGRDHSTVIYANNKIRKLLETDSRLKATLQEIEDQLLKKN

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=454, Percent_Identity=39.2070484581498, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI226510964, Length=229, Percent_Identity=25.764192139738, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52282; Mature: 52282

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRYCIVDNLWEESIKIIKEKISPQNFETWIRPLKVVSLEGNHASLSVPNKFFKDWLTEN
CCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEECCCCCEECCCHHHHHHHHHHH
YRDVISDAVSSSAGKDYAIDFVILQESEKNSRSAHPARKKKIIEQIPQEKIRPKIHPTLN
HHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCC
PNYSFERFVVGSSNQFAHAAAVAVAEQPAKNYNPLFIYGGVGLGKTHLLNAIGLLSMSIY
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHEEC
PDMNVVYVSAEEFMNELILSIRYDKMPQFREKFRNIDCLLMDDIQFIAGKERTQEEFFHT
CCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCHHHHHCCCHHHHHHHHH
FNTLHDSGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLIADIQPPEIETKIAILEKKAQEN
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCC
NIHIPTNVAYYISSHAESNIRELEGFLVRIAAYSSVTGRNIDLDLVKEVLKDIIKHRESD
CEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
EISFEEILKAVAARYNLKIADIKSQSKNKNIAMARQVTMYMARKLTGSSFPDIGEKIGGR
CCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCC
DHSTVIYANNKIRKLLETDSRLKATLQEIEDQLLKKN
CCCEEEEECHHHHHHHHCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRYCIVDNLWEESIKIIKEKISPQNFETWIRPLKVVSLEGNHASLSVPNKFFKDWLTEN
CCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEECCCCCEECCCHHHHHHHHHHH
YRDVISDAVSSSAGKDYAIDFVILQESEKNSRSAHPARKKKIIEQIPQEKIRPKIHPTLN
HHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCC
PNYSFERFVVGSSNQFAHAAAVAVAEQPAKNYNPLFIYGGVGLGKTHLLNAIGLLSMSIY
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHEEC
PDMNVVYVSAEEFMNELILSIRYDKMPQFREKFRNIDCLLMDDIQFIAGKERTQEEFFHT
CCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCHHHHHCCCHHHHHHHHH
FNTLHDSGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLIADIQPPEIETKIAILEKKAQEN
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCC
NIHIPTNVAYYISSHAESNIRELEGFLVRIAAYSSVTGRNIDLDLVKEVLKDIIKHRESD
CEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
EISFEEILKAVAARYNLKIADIKSQSKNKNIAMARQVTMYMARKLTGSSFPDIGEKIGGR
CCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCC
DHSTVIYANNKIRKLLETDSRLKATLQEIEDQLLKKN
CCCEEEEECHHHHHHHHCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA