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The map label for this gene is lpsJ [H]

Identifier: 85375687

GI number: 85375687

Start: 2872901

End: 2873524

Strand: Reverse

Name: lpsJ [H]

Synonym: ELI_14300

Alternate gene names: 85375687

Gene position: 2873524-2872901 (Counterclockwise)

Preceding gene: 85375688

Following gene: 85375686

Centisome position: 94.14

GC content: 62.02

Gene sequence:

>624_bases
ATGGCCGCCCGCGCTGCGCAGGAGCTGGAGGATGGCTATTACGTCAATCTCGGCATCGGCATCCCGACGCTGGTCGCCAA
CCACATCCCGGACGGCATGCAGGTCACCTTGCAGAGCGAGAACGGCATGCTCGGCATCGGGCCCTTTCCTTATGATGACG
AAGTCGATCCCGACCTTATCAATGCCGGGAAGCAGACCATCAGCGAGCTGGCCCACAGCGCCTATTTCGACAGCGCCACC
AGCTTCGGCATGATCCGCGGCGGCCATATCGACCTGACCGTACTCGGGGCGATGGAGGTAGCGGAGAACGGCGACATCGC
CAACTGGATGATCCCCGGCAAGATGATCAAGGGCATGGGCGGCGCGATGGACCTGGTCGCCGGGGTGAAGAAAATCATCG
TCGTGATGGATCACACCAGCAAGGCGGGCGATCCCAAGTTCATCCCGGAATGCACGCTGCCGCTGACCGGCACCAATGTC
GTCGACATGATCATCACCAATCTGGGCGTGTTTCACCGGGTCGATCACGACAGCCCCTTCCGCCTGATCGAACTGGCGGA
AGGCGTGACGGCAGACGAAGTTGCCGCCAGCACGACCGCGAAATACGAGGTCGCGCTCGGCTGA

Upstream 100 bases:

>100_bases
CCGACGCTGCGTTCGGGCTCGATGAAATGCTCGGCCAGGGCTGGTTCCTGGCCCCGATTCCCGCAGAAGGACAAACACAA
TGAGCTGGACCCGTGACCAA

Downstream 100 bases:

>100_bases
TCCGCCATGTGGCTGACCGAACCGCGCAATGCCCATTCTCCGCTCGCCGGGCTCAAGGTGCTCGAGCTGGCGCGCGTTTT
GGCGGGGCCGTTCTGCGGAC

Product: 3-oxoadipate CoA-transferase, beta subunit

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MAARAAQELEDGYYVNLGIGIPTLVANHIPDGMQVTLQSENGMLGIGPFPYDDEVDPDLINAGKQTISELAHSAYFDSAT
SFGMIRGGHIDLTVLGAMEVAENGDIANWMIPGKMIKGMGGAMDLVAGVKKIIVVMDHTSKAGDPKFIPECTLPLTGTNV
VDMIITNLGVFHRVDHDSPFRLIELAEGVTADEVAASTTAKYEVALG

Sequences:

>Translated_207_residues
MAARAAQELEDGYYVNLGIGIPTLVANHIPDGMQVTLQSENGMLGIGPFPYDDEVDPDLINAGKQTISELAHSAYFDSAT
SFGMIRGGHIDLTVLGAMEVAENGDIANWMIPGKMIKGMGGAMDLVAGVKKIIVVMDHTSKAGDPKFIPECTLPLTGTNV
VDMIITNLGVFHRVDHDSPFRLIELAEGVTADEVAASTTAKYEVALG
>Mature_206_residues
AARAAQELEDGYYVNLGIGIPTLVANHIPDGMQVTLQSENGMLGIGPFPYDDEVDPDLINAGKQTISELAHSAYFDSATS
FGMIRGGHIDLTVLGAMEVAENGDIANWMIPGKMIKGMGGAMDLVAGVKKIIVVMDHTSKAGDPKFIPECTLPLTGTNVV
DMIITNLGVFHRVDHDSPFRLIELAEGVTADEVAASTTAKYEVALG

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=203, Percent_Identity=56.1576354679803, Blast_Score=229, Evalue=1e-60,
Organism=Homo sapiens, GI11545841, Length=203, Percent_Identity=56.1576354679803, Blast_Score=228, Evalue=4e-60,
Organism=Escherichia coli, GI1788552, Length=205, Percent_Identity=51.7073170731707, Blast_Score=216, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17531517, Length=206, Percent_Identity=52.9126213592233, Blast_Score=222, Evalue=1e-58,
Organism=Drosophila melanogaster, GI21358325, Length=206, Percent_Identity=57.7669902912621, Blast_Score=247, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24655794, Length=206, Percent_Identity=57.7669902912621, Blast_Score=246, Evalue=8e-66,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 21945; Mature: 21814

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARAAQELEDGYYVNLGIGIPTLVANHIPDGMQVTLQSENGMLGIGPFPYDDEVDPDLI
CCCCHHHHHCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCCHHHH
NAGKQTISELAHSAYFDSATSFGMIRGGHIDLTVLGAMEVAENGDIANWMIPGKMIKGMG
HHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEEEEEEEECCCCCCEEEEEECHHHHHCCC
GAMDLVAGVKKIIVVMDHTSKAGDPKFIPECTLPLTGTNVVDMIITNLGVFHRVDHDSPF
CHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCH
RLIELAEGVTADEVAASTTAKYEVALG
HHHHHHCCCCHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
AARAAQELEDGYYVNLGIGIPTLVANHIPDGMQVTLQSENGMLGIGPFPYDDEVDPDLI
CCCHHHHHCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCCHHHH
NAGKQTISELAHSAYFDSATSFGMIRGGHIDLTVLGAMEVAENGDIANWMIPGKMIKGMG
HHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEEEEEEEECCCCCCEEEEEECHHHHHCCC
GAMDLVAGVKKIIVVMDHTSKAGDPKFIPECTLPLTGTNVVDMIITNLGVFHRVDHDSPF
CHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCH
RLIELAEGVTADEVAASTTAKYEVALG
HHHHHHCCCCHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]