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The map label for this gene is hisF2 [H]

Identifier: 85375494

GI number: 85375494

Start: 2687064

End: 2687858

Strand: Reverse

Name: hisF2 [H]

Synonym: ELI_13335

Alternate gene names: 85375494

Gene position: 2687858-2687064 (Counterclockwise)

Preceding gene: 85375495

Following gene: 85375493

Centisome position: 88.06

GC content: 61.13

Gene sequence:

>795_bases
ATGCGCCGCCTTCGAATTATTCCGGTCCTGTTGCTTGATGGAACAAGCGCAGTAAAGACCCACCAGTTCAAACCTGCGCG
CTATATCGGCGACATCGTGAACGCTGCGCAGATCTTCAACAGCAAGATGGCCGACGAGCTGATCGTAGTCGATATCGGAT
ACCACCAAGACAATCCATCGCGTCCGGAAGTTATCCGGCACTTGGCTGAAGAATGCTTTATGCCACTGGCCTTTGGTGGT
GGCATTTCCAGCGTCGATGAAGCCGCCGCCCTGTTCAAGGACGGCATTGAGAAGGTTTCCATCAATACTGCCTTCGTCGA
AGGCGGAGCGCTTGTCGAAGAGGCGGCCAGGCAGTTCGGCAGCCAGAGCGTCGTCGCCTCGATCGACGCCCGGCGCGAGG
CCGATGGCAGCTACCAGGTCTACAGTCGCGGCGGCACCCGCGCTCTCGGGATCGATCCTGTCGAAATGGCCAGGCGAGCG
GAGGCCCAGGGGGCCGGCGAAATTCTCATCCAGTCGATCGACCGGGATGGTATGCGTAAAGGATATGACAGGAACCTGGT
GCGATCGATCGTCGACGCGACGACGCTGCCGGTCGTGGCCCTGGGCGGCGCCGACACGGTCGACGATCTCGCTCAGGTCA
TCCTGCGGGACGGCGCGTCCGCAGCAGCAGCCGGGTCCATGTTCGCATTTTACGGACGGCTGCAGGCGGTGCTCATCAAC
TATCCGCGTCCGGAAGAGCGCGATGCTGCAGCAGCACGCGCGAATATCGGCGAAGAGACGATGGCCGAGGCATGA

Upstream 100 bases:

>100_bases
TGGCCGGATGCGCGCGCGACAACGTCATGGGGGTGCAGTTCCATCCGGAGAAAAGCCACATGTTCGGTGTGGAAATACTT
ACCAATTTCGCGGGGATTTG

Downstream 100 bases:

>100_bases
CAAGCGAGTACCGGATGTGCAGCCGGACGGTCATGGATACGACCGACCCAGCCATCACTTTCGACGCCGATGGCGTCAGC
AACCACTTCTGGCACGCACA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF2; ImGP synthase subunit hisF2; IGPS subunit hisF2 [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRRLRIIPVLLLDGTSAVKTHQFKPARYIGDIVNAAQIFNSKMADELIVVDIGYHQDNPSRPEVIRHLAEECFMPLAFGG
GISSVDEAAALFKDGIEKVSINTAFVEGGALVEEAARQFGSQSVVASIDARREADGSYQVYSRGGTRALGIDPVEMARRA
EAQGAGEILIQSIDRDGMRKGYDRNLVRSIVDATTLPVVALGGADTVDDLAQVILRDGASAAAAGSMFAFYGRLQAVLIN
YPRPEERDAAAARANIGEETMAEA

Sequences:

>Translated_264_residues
MRRLRIIPVLLLDGTSAVKTHQFKPARYIGDIVNAAQIFNSKMADELIVVDIGYHQDNPSRPEVIRHLAEECFMPLAFGG
GISSVDEAAALFKDGIEKVSINTAFVEGGALVEEAARQFGSQSVVASIDARREADGSYQVYSRGGTRALGIDPVEMARRA
EAQGAGEILIQSIDRDGMRKGYDRNLVRSIVDATTLPVVALGGADTVDDLAQVILRDGASAAAAGSMFAFYGRLQAVLIN
YPRPEERDAAAARANIGEETMAEA
>Mature_264_residues
MRRLRIIPVLLLDGTSAVKTHQFKPARYIGDIVNAAQIFNSKMADELIVVDIGYHQDNPSRPEVIRHLAEECFMPLAFGG
GISSVDEAAALFKDGIEKVSINTAFVEGGALVEEAARQFGSQSVVASIDARREADGSYQVYSRGGTRALGIDPVEMARRA
EAQGAGEILIQSIDRDGMRKGYDRNLVRSIVDATTLPVVALGGADTVDDLAQVILRDGASAAAAGSMFAFYGRLQAVLIN
YPRPEERDAAAARANIGEETMAEA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=230, Percent_Identity=31.304347826087, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI87082028, Length=214, Percent_Identity=28.5046728971963, Blast_Score=67, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319725, Length=258, Percent_Identity=30.2325581395349, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR020021
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28297; Mature: 28297

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLRIIPVLLLDGTSAVKTHQFKPARYIGDIVNAAQIFNSKMADELIVVDIGYHQDNPS
CCCEEEEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
RPEVIRHLAEECFMPLAFGGGISSVDEAAALFKDGIEKVSINTAFVEGGALVEEAARQFG
HHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHCHHEEEEEHEEECCCHHHHHHHHHHC
SQSVVASIDARREADGSYQVYSRGGTRALGIDPVEMARRAEAQGAGEILIQSIDRDGMRK
CCHHHHHHHHHHCCCCCEEEEECCCCEEECCCHHHHHHHHHCCCHHHHHHHHHCHHHHHC
GYDRNLVRSIVDATTLPVVALGGADTVDDLAQVILRDGASAAAAGSMFAFYGRLQAVLIN
CCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEE
YPRPEERDAAAARANIGEETMAEA
CCCCCCHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MRRLRIIPVLLLDGTSAVKTHQFKPARYIGDIVNAAQIFNSKMADELIVVDIGYHQDNPS
CCCEEEEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
RPEVIRHLAEECFMPLAFGGGISSVDEAAALFKDGIEKVSINTAFVEGGALVEEAARQFG
HHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHCHHEEEEEHEEECCCHHHHHHHHHHC
SQSVVASIDARREADGSYQVYSRGGTRALGIDPVEMARRAEAQGAGEILIQSIDRDGMRK
CCHHHHHHHHHHCCCCCEEEEECCCCEEECCCHHHHHHHHHCCCHHHHHHHHHCHHHHHC
GYDRNLVRSIVDATTLPVVALGGADTVDDLAQVILRDGASAAAAGSMFAFYGRLQAVLIN
CCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEE
YPRPEERDAAAARANIGEETMAEA
CCCCCCHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8939432; 12057956; 10984043 [H]