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The map label for this gene is hisH2 [H]

Identifier: 85375495

GI number: 85375495

Start: 2687858

End: 2688532

Strand: Reverse

Name: hisH2 [H]

Synonym: ELI_13340

Alternate gene names: 85375495

Gene position: 2688532-2687858 (Counterclockwise)

Preceding gene: 85375500

Following gene: 85375494

Centisome position: 88.08

GC content: 57.48

Gene sequence:

>675_bases
GTGAAACAAGAAGACGTGCTCGACAAGGACGCGGTGCTTGAAACCGAGGCCAATCACATCGGCATTATCGACTACGGCAT
CTGCAATGTCGGTTCGGTCCGCAACATGTTGCGTCGGTCCGGTATCCTCGCCAGCTGTATTTCGTCGCCCGATGAAATGG
ACGCCTGCGACCGGATCATACTTCCAGGGGTGGGCCATTTCAGCGCTGGCATGGCAAACCTAGAAAAAGGTGGCTGGATA
GACCCGATCCGGCATGCTGCGCTCAAACAGCGCAAGCCGATCCTGGGCATTTGCCTCGGGATGCAATTGCTTGCCCACCA
CAGCGAGGAAGGCGATTGCGAAGGCCTTGGCCTGCTGGATATGGATGTGAAACACTTCGACCGGTCGCGGCTCGGGCCCA
GATTGCCGATCCCGCATATGGGCTGGTCGGAAGTGGATGCAGACCATGATCATCCGCTCACGCACAGCGAATTGCCTGAA
AAGCAGCGCTTCTATTTCGTGCACAGCCTGCATGTGGTGCCGCGCAGCGAGAAGGTGCAAGCCCTGTTCTGGTGCAACTA
CGGCTATCCCTTCGTGGCCGGATGCGCGCGCGACAACGTCATGGGGGTGCAGTTCCATCCGGAGAAAAGCCACATGTTCG
GTGTGGAAATACTTACCAATTTCGCGGGGATTTGA

Upstream 100 bases:

>100_bases
ACGCCCCATGACCGCCCATTGGGCTGGCGACGGGATAATGCCCCTTGAGATTAAATCAATAGCAGGTATTGGCCGAGCGC
CGCTGCAGTAGGGGATTCAG

Downstream 100 bases:

>100_bases
TGCGCCGCCTTCGAATTATTCCGGTCCTGTTGCTTGATGGAACAAGCGCAGTAAAGACCCACCAGTTCAAACCTGCGCGC
TATATCGGCGACATCGTGAA

Product: glutamine amidotransferase

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 2; IGP synthase subunit hisH 2; ImGP synthase subunit hisH 2; IGPS subunit hisH 2 [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKQEDVLDKDAVLETEANHIGIIDYGICNVGSVRNMLRRSGILASCISSPDEMDACDRIILPGVGHFSAGMANLEKGGWI
DPIRHAALKQRKPILGICLGMQLLAHHSEEGDCEGLGLLDMDVKHFDRSRLGPRLPIPHMGWSEVDADHDHPLTHSELPE
KQRFYFVHSLHVVPRSEKVQALFWCNYGYPFVAGCARDNVMGVQFHPEKSHMFGVEILTNFAGI

Sequences:

>Translated_224_residues
MKQEDVLDKDAVLETEANHIGIIDYGICNVGSVRNMLRRSGILASCISSPDEMDACDRIILPGVGHFSAGMANLEKGGWI
DPIRHAALKQRKPILGICLGMQLLAHHSEEGDCEGLGLLDMDVKHFDRSRLGPRLPIPHMGWSEVDADHDHPLTHSELPE
KQRFYFVHSLHVVPRSEKVQALFWCNYGYPFVAGCARDNVMGVQFHPEKSHMFGVEILTNFAGI
>Mature_224_residues
MKQEDVLDKDAVLETEANHIGIIDYGICNVGSVRNMLRRSGILASCISSPDEMDACDRIILPGVGHFSAGMANLEKGGWI
DPIRHAALKQRKPILGICLGMQLLAHHSEEGDCEGLGLLDMDVKHFDRSRLGPRLPIPHMGWSEVDADHDHPLTHSELPE
KQRFYFVHSLHVVPRSEKVQALFWCNYGYPFVAGCARDNVMGVQFHPEKSHMFGVEILTNFAGI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=204, Percent_Identity=38.2352941176471, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=211, Percent_Identity=32.7014218009479, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 24976; Mature: 24976

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQEDVLDKDAVLETEANHIGIIDYGICNVGSVRNMLRRSGILASCISSPDEMDACDRII
CCCCHHCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHCEEE
LPGVGHFSAGMANLEKGGWIDPIRHAALKQRKPILGICLGMQLLAHHSEEGDCEGLGLLD
CCCCCCHHHCHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEE
MDVKHFDRSRLGPRLPIPHMGWSEVDADHDHPLTHSELPEKQRFYFVHSLHVVPRSEKVQ
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEEEECCCCCCEE
ALFWCNYGYPFVAGCARDNVMGVQFHPEKSHMFGVEILTNFAGI
EEEEECCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQEDVLDKDAVLETEANHIGIIDYGICNVGSVRNMLRRSGILASCISSPDEMDACDRII
CCCCHHCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHCEEE
LPGVGHFSAGMANLEKGGWIDPIRHAALKQRKPILGICLGMQLLAHHSEEGDCEGLGLLD
CCCCCCHHHCHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEE
MDVKHFDRSRLGPRLPIPHMGWSEVDADHDHPLTHSELPEKQRFYFVHSLHVVPRSEKVQ
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEEEECCCCCCEE
ALFWCNYGYPFVAGCARDNVMGVQFHPEKSHMFGVEILTNFAGI
EEEEECCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8939432; 12057956; 10984043 [H]